ANITA ROBINSON_CSECBIO2024 GROUP A

Candidates Name: Robinson Anita, Patricia

Practical#: 19
Date
Title: Sugar Test {Reducing and Non-Reducing Sugars}
Problem Statement: Supermarkets offer a choice amongst apple juice, apple drinks and
apple soda. Plan and design an experiment to determine the sugar
content of apple juice, apple drinks and apple soda.
Hypothesis: There is a higher sugar content in apple soda than in apple drinks and
apple juice.
Aim: To determine the sugar content level in apple soda, apple drinks and
apple juice.
Apparatus: Glucose Solution, Benedict’s Solution, Sucrose Solution, Hydrochloric
Acid, Sodium Bi-carbonate, test tube, distilled water, dropper,
measuring cylinder, beaker, apple juice, apple drink, apple soda
Method: Testing for Reducing Sugar
1. Pour 2cm of glucose solution into a test tube labelled A containing
apple soda.
2. Add 1cm of benedict’s solution to the test tube and shake gently.
3. Place the test tube in a water bath.
4. Repeat steps 1-3 for test tube B containing apple drink and test tube
C containing apple juice respectively.
Testing for Non-reducing Sugar
1. Pour 2cm of sucrose solution into each test tube A, B and C.
2. Test the contents of test tube A for non-reducing sugar.
3. Add 1cm of hydrochloric acid to the test tube and place in a water
bath for 5 minutes.
4. Remove the test tube from the water bath and add sodium
bicarbonate drop by drop until frizzing stops.
5. Add 2cm of benedict’s solution and gently shake the test tube.
6. Place the test tube in a water bath for 5 minutes.
7. Repeat steps three to seven for test tube B containing apple drink
and C containing apple juice respectively.
ANITA ROBINSON_CSECBIO2024 GROUP A

Expected Results:
TABLE SHOWING OBSERVATION TAKEN FROM TESTING FOR REDUCING
AND NON-REDUCING SUGAR IN APPLE JUICE, APPLE SODA AND APPLE
DRINKS

TESTS APPLE SODA APPLE DRINK APPLE JUICE

REDUCING
SUGARS
NON-REDUCING
SUGARS

Interpretation of Results: The results after testing apple soda, apple drink and apple juice
for reducing sugar turns a bright orange in colour indicating
that the test is positive. The results for testing for non-reducing
sugar were negative therefore the colour of the benedict’s
solution didn’t change.
Limitations: 1. Stopwatch malfunction
2. Over pouring of chemicals
3. Contamination of chemicals
Source of Error: The water bath temperature was too high, which equated in a
rapid colour change in all the apple solutions causing the results
to be difficult to determine.
Precautions: 1. Ensure that the stopwatch is fully functional
2. Ensure that accurate measurements of chemicals are taken
3. Ensure that the equipment is thoroughly cleaned