Modified date: 2023-12-08

Alcohol in solid sample

Standard and chemical:

Standard: Ethanol
IS: n-butanol
10000ppm standard stock solution: weight 1ml ethanol into 100ml v. flask.
10000ppm Internal standard solution (IS): pipette 1ml n-butanol into 100ml v. flask.
Standard Conc. IS conc Stock solution vol. IS vol. Make up vol.
(mg/L) (mg/L) (ml) (ml) (ml)
10 100 0.01 0.1 10
20 100 0.02 0.1 10
50 100 0.05 0.1 10
100 100 0.1 0.1 10
500 100 0.5 0.1 10

Sample preparation:
1. Weigh 10.0g sample in digestion tube.
2. Distillate and collect about 220 mL solution into 250ml measuring cylinder (~around 8mins). (No
need to add alkaline)
3. Transfer to 250mL v. flask and make up to mark with water.
4. Dilute sample solution if necessary.
5. Filter diluted sample solution by 0.45um Nylon.

Program & GC Condition:

Column: *Wax (First priority) /Wax plus /624
250.0 um x 30.0 m x 0.25 um
Injection vol.: 2uL
Wash bottle: A (D.I. water) B (Acetone)
3 times 3 times
Front Inlet: Mode: Split
Heater: 240oc
Split Ratio: 50:1
Split flow: 50ml/min
Oven program: Flow Rate ( c/min) Temperature (oc)
o
Hold time (min)
50 6
20 130 5
Run time: 15 mins
Equilibration time: 1 min
Detector: FID
Heater: 220oc
 Modified date: 2023-12-08

QC:

Method blank, duplicate, spike

Spike: 10000ppm 0.5ml into sample, 2 trials. (by sample 400ppm, by solution 20ppm)

Remarks:

Distillation unit: DON’T use Ethanol to rinse /wash the system, use alkaline.

GC method name:

1. FD_alcohol2020.M (GC-FID-002)