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Basics of MRS I
Basics of MRS I
Cho
Cr NAA
Lactate
ppm
Lecture Outline Part I: Chemical Shift J-coupling Localization Parameters Part II Spectroscopic Imaging Processing Quantification
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Basics of MRS
MRS looks at the chemical composition of the tissue of interest and displays it in a spectrum
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Larmor Frequency
f0 = * x B0
B0
( * = ) 2
Lamor frequency:
1.5 T
1H 31P
Basics of MR Spectroscopy
What is necessary to obtain an MR signal? magnetic moment 0 high gyromagnetic ratio => enough sensitivity natural abundance of the isotope concentration mmol/l in the body
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H C N 0
1.00 1.8 10
-4
13 14
17 19 23
Na P
31 39 43
Ca
9.3 10-6
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metabolites: Problems:
1-11 mmol/l in the body, high sensitivity water suppression (110 mol/l !!), fat suppression overlapping of metabolite peaks
31P
~ 10 mmol/l, important for studying energy metabolism Problems: low sensitivity (100* lower than 1H MRS) basic atom in organic molecules Problems: only 1% natural abundance of 13C => very low sensitivity
13C
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1.5 T
63.86 MHz 25.85 MHz
3T
127.73 MHz 51.7 MHz
RF Excitation pulse
FT
f0
FT
ms
Hz / ppm
Time domain
Frequency domain
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FT
ms
Hz / ppm
Time domain
Frequency domain
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Fourier Transform
NAA
Spectrum FID
Cre t Cho Cho Cre NAA f
Time domain FT
Frequency domain
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MR Spectroscopy
H2 O
MRS Experiment
MR Spectroscopy
BUT:
CH3CH2OH
Looking more closely there are more peaksrevealing the real nature of the drink
CH2 CH3
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1H
MR Spectroscopy in vivo
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Chemical Shift
H2O O H H
Different chemical environment of 1H nuclei => slightly different local magnetic field => slightly different Larmor frequency f Resonance frequency shift is called chemical shift Causes of chemical shift: shielding/deshielding of the electron shell J-coupling/spin-spin coupling (effect of neighbour)
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Chemical Shift
Chemical environment shielding
water
fat
O H
ion bonding H deprived from electron weak shielding => sense higher B0-field
H H
C H
covalent bonding shared electrons strong shielding => sense lower B0-field
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Chemical shift
[Hz] = f - fref
H2O unit: ppm (parts per million = 10-6)
[ppm] =
f - fref fref
106
1.5 T: = 177 Hz 3 T: = 354 Hz
NAA
= 2.7 ppm
1H: 31P:
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Chemical shift
IMPORTANT: The chemical shift expressed in ppm is independent of the magnetic field strength ! - e.g. NAA is always at 2 ppm The value of 1ppm in Hz is different at different field strength and for different nuclei:
1.5 T 1H : 31P : 1ppm = 63.87 Hz 1ppm = 25.86 Hz 3T 1ppm = 127.74 Hz 1ppm = 51.72 Hz
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Hz Scaling
3T
-100
-200
-300
-400
-500
Hz
1.5T
-100
-200
-300 Frequency
-400
-500
Hz
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ppm Scaling
1.5T 3T
NAA
Cho tCr
NAA
Glx Gln
NAA
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J-coupling
Line splitting due to influence of neighboring nuclei:
Example: Lactate
or
H C-C-CH3
OH
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J-coupling
Fine structure
1H SPECTRUM OF LACTATE O H C-C-CH3 CH3 O OH
OH rest CH
3 1
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J-coupling
Fine structure
1H SPECTRUM OF LACTATE O H C-C-CH3 CH3 O OH
OH rest CH
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J-coupling
Fine structure
1H SPECTRUM OF LACTATE O H C-C-CH3 CH3 O OH
OH rest CH
1:1
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J-coupling
Fine structure
1H SPECTRUM OF LACTATE O H C-C-CH3 CH3 O OH
OH rest CH
1:1
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J-coupling
Fine structure
1H SPECTRUM OF LACTATE O H C-C-CH3 CH3 O OH
OH rest CH
1:1 1:3:3:1
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J-coupling
Fine structure
1H SPECTRUM OF LACTATE
H C-C-CH3
OH
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J-coupling
Fine structure
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J-coupling
Fine structure
down at TE = n/J
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~ 6-12 mM
CH3 CH2 CH
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Function:
1. Neuronal marker i.e. concentration correlates with neuronal density 2. Neuronal function, i.e. synthesis is energy-dependent, therefore concentration correlates with neuronal function
Tumor, Stroke, Epilepsy, Hyp-/Anoxia, Inflammation, Dementia, Trauma Brain Development and Maturation Canavans Disease (aspartoacylase deficiency)
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Example: Epilepsy
Right hemisphere
Left hemisphere
4.0
3.0
2.0
1.0
4.0
3.0
2.0
1.0
NAA
Courtesy: Dept. of Radiology, University of Bonn, Germany
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~ 6-12 mM
H3C-N-CH2-COOC=NH2+ NH2
CH3 CH2
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Function:
1. Energy Buffer: H + PCr + ADP ATP + Cr 2. Energy shuttle: Energy transport from production (mitochondria) to energy utilizing sites
The CRE peak is often stable and therefore may serve as an internal reference
Exceptions: Cr/PCr Decrease: Acute and subacute stroke, Brain tumor, Brain metastasis, Abscesses, Inborn errors of Creatine synthesis
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Example: Abcess
TE 35 ms
Elevated lactate Elevated amino acids around lactate Near absence of metabolites
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~ 2 mM
CH3 CH2
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Function:
Involved in pathways of phospholipid synthesis and degradation. => reflecting membrane synthesis and degradation
Cho Increase:
Brain Tumors, MS-Plaques, Stroke, Inflammation, White Matter Diseases Hepatic Encephalopathy, Necrosis
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Cho Decrease:
Lactate
TE = 144 ms = 1/J
~ 1 mM
O H C-C-CH3 O OH
TE = 288 ms = 2/J
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Lactate = Lac
Function:
Sign of impaired energy metabolism, impaired oxygen delivery (anaerobic glycolysis). Not or hardly detectable in normal brain tissue (~1 mM)
Lac Increase:
Stroke, An-/Hypoxia, Mitochondrial diseases, Tumors (Brain and Metastases), Epileptic discharges, Abscesses/Infection, Prolonged neuronal activation (?)
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Lactate Cr NAA
ppm 4
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J (Lac) = 6.933 Hz => multiplet looks up at down at TE = n/J TE = n/J with even n (288 ms, 576 ms, ) with odd n (144 ms, 432 ms, )
Glioblastoma Multiforme
Cho
TE 35 ms
mI Cr NAA
Lactate/Lipid
ppm 4
Glioblastoma Multiforme
Cho
TE 288 ms
Lactate Cr NAA
ppm 4
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f = 1 / T acq
f = BW / N
Tacq
Bandwidth
Spectral resolution f = 1 / T acq Spectral resolution f = BW / samples => acquisition time depends on spectral resolution!
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Localization
brain (hippocampus)
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Subacute
Normal
Acute
Normal
FLAIR - ROIs
Courtesy: Howard Rowley
2D CSI
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Cho
NAA Lac
Cre
Localization
prostate
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Localization by gradients
in each direction a gradient field defines one slice => the intersection of these slices is the selected volume of interest
These methods can also be used in addition to a localization with a surface coil to profit from high SNR.
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Multivoxel techniques:
Spectroscopic Imaging / Chemical Shift Imaging (CSI) Hadamard encoding
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Localization: PRESS
PRESS = Point RESolved Spectroscopy double spin echo sequence: 90 - 180 - 180 - echo acquisition
Gx
Gy
Gz
90
o
180o
180 o
Rf
time
TE
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Localization: PRESS
PRESS = Point RESolved Spectroscopy
RF INPUT
RF OUTPUT
FID
G 3
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Localization: PRESS
PRESS = Point RESolved Spectroscopy
TE1/2
RF INPUT
T2
RF OUTPUT
FID
first echo
3
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Localization: PRESS
PRESS = Point RESolved Spectroscopy
TE1/2 (TE1+TE2)/2 TE1/2
RF INPUT
T2
RF OUTPUT
FID
first echo second echo
3
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Disadvantages:
minimal TE: ~ 19 ms => unsuitable for 31P spectroscopy
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Localization: STEAM
STEAM = Stimulated Echo Acquisition Mode 3 slice selective 90 pulses => stimulated echo after TE + TM
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Localization: STEAM
RF INPUT RF OUTPUT
FID
G3
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Localization: STEAM
RF INPUT RF OUTPUT
FID
G3
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Localization: STEAM
RF INPUT
T2
RF OUTPUT
FID
second echo
G3
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Localization: STEAM
Advantages:
allows shorter echo times than PRESS (~10 ms) better water suppression than PRESS less chemical shift artefacts (no 180 refocusing pulses)
Disadvantages:
signal intensities are only half of those obtained with PRESS
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finally
intensity arbitrary units
NAA
NAA
Glx Gln
Chemical shift
ppm
Shimming Purpose of shimming: Reduce inhomogeneities => longer T2* => smaller linewidth and higher amplitudes This improves
signal to noise resolution
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Shimming
T2 *
t f
FT
f =
1 T2 *
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Shimming
FT
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Shimming
FT
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Shimming
FT
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Effect of Shimming
Example: prostate spectrum
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Effect of Shimming
Example: prostate spectrum
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Effect of Shimming
Example: prostate spectrum
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Effect of Shimming
Example: prostate spectrum
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Thank you!
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