TH2019PESC2044

Contribution à la modélisation d’interface biologique par
spectroscopie d’impédance : application au suivi de

l’épithélium pigmenté de la rétine durant sa croissance
et face à diverses perturbations
Jocelyn Boutzen
To cite this version:

Jocelyn Boutzen. Contribution à la modélisation d’interface biologique par spectroscopie d’impédance :
application au suivi de l’épithélium pigmenté de la rétine durant sa croissance et face à diverses
perturbations. Optique / photonique. Université Paris-Est, 2019. Français. �NNT : 2019PESC2044�.
�tel-02482728v2�
HAL Id: tel-02482728

https://theses.hal.science/tel-02482728v2
Submitted on 18 Feb 2020
HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est

archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents
entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non,
lished or not. The documents may come from émanant des établissements d’enseignement et de
teaching and research institutions in France or recherche français ou étrangers, des laboratoires
abroad, or from public or private research centers. publics ou privés.
(𝑯𝟐𝑶𝟐

𝑆
10−13
𝑚²
𝑅𝑇 𝐶𝑖𝑛𝑡
𝑉𝑚𝑒𝑚𝑏 = 𝑉𝑖𝑛𝑡 − 𝑉𝑒𝑥𝑡 = − ln ( )
𝑧𝐹 𝐶𝑒𝑥𝑡
𝐶𝑖𝑛𝑡
𝐶𝑒𝑥𝑡
𝑅𝑇 ∑𝑐 𝑝𝑐 𝐶𝑐,𝑒𝑥𝑡 + ∑𝑎 𝑝𝑎 𝐶𝑎,𝑖𝑛𝑡
𝑉𝑚𝑒𝑚𝑏 = 𝑉𝑖𝑛𝑡 − 𝑉𝑒𝑥𝑡 = − ln ( )
𝐹 ∑𝑐 𝑝𝑐 𝐶𝑐,𝑖𝑛𝑡 + ∑𝑎 𝑝𝑎 𝐶𝑎,𝑒𝑥𝑡
𝑝𝑐/𝑎
𝐶𝑐/𝑎,𝑖𝑛𝑡/𝑒𝑥𝑡
𝑚3 )


𝑔𝑚𝑒𝑚𝑏
𝜌 𝐶=
10−2 𝐹/𝑚², 𝑔𝑚𝑒𝑚𝑏 = 5 𝑆/𝑚² 𝑒𝑡 𝜌 = 1 Ω𝑚



𝑑
𝜃 = arctan( )
𝑓





𝑜𝑂𝑥 + 𝑧𝑒 − = 𝑟𝑅𝑒𝑑
𝑜
𝑜 𝑅𝑇 𝑎𝑂𝑥
𝐸 = 𝐸𝑂𝑥/𝑅𝑒𝑑 + ln ( 𝑟 )
𝑧𝐹 𝑎𝑅𝑒𝑑
𝑎𝑂𝑥 /𝑎𝑅𝑒𝑑
𝑜
𝐸𝑂𝑥/𝑅𝑒𝑑
𝐴𝑔𝐶𝑙(𝑠) + 𝑒 − = 𝐴𝑔(𝑠) + 𝐶𝑙 −
𝑜 𝑅𝑇
𝐸 = 𝐸𝐴𝑔𝐶𝑙 ⁄𝐶𝑙 − − ln([𝐶𝑙 − ])
𝐹
𝑜
𝐸𝐴𝑔𝐶𝑙 ⁄𝐶𝑙 − vs ESH = 0.222 𝑉
𝐻+

𝑉𝑅𝐸/𝑀
𝑉𝑅𝐸/𝑀 = −𝑉𝑒
𝑉𝐸𝑇/𝑀 = 0
𝑉𝐸𝑇/𝑅𝐸 = 𝑉𝐸𝑇/𝑀 − 𝑉𝑅𝐸/𝑀 = 𝑉𝑒 .
𝑉𝐸𝑇/𝑅𝐸 = 𝑉𝑒
𝑉𝐶𝐸/𝑀 = −𝑉𝑒 𝑉𝐸𝑇/𝑀 = 0
𝑉𝐸𝑇/(𝐶𝐸=𝑅𝐸) = 𝑉𝑒
𝑉𝑒
𝑉𝑚




𝑟 𝜎
1
𝑅𝑒 =
4𝜎𝑟

𝜀0 𝜀
𝐶𝑠 = 𝑒
𝜀0 𝜀
e = 1 nm, 𝜀0 = 8.85. 10−12 𝐹/𝑚 𝜀 = 78.5
1
𝑍𝐶 = 𝑗𝐶𝜔 𝜔
1
𝑍𝐶𝑃𝐸 = (𝑗𝜔)𝛼 𝑄
1
𝑍𝐶𝑃𝐸 = 0<𝛽<1
(𝑗𝜔)1−𝛽𝑄
−𝛼90°
–𝛼 𝛼
𝐹. 𝑠 𝛼−1 0<𝛼<1
𝛼=1
𝛼 𝛼 = 0.99
𝛼
𝜀𝑠 − 𝜀∞
𝜀(𝜔) = 𝜀𝑖𝑛𝑓 +
1 + 𝑗𝜔𝜏0
𝜏0 𝜀∞
1
𝜀𝑠
𝜏0
𝜀𝑠 − 𝜀∞
𝜀(𝜔) = 𝜀 ′ + 𝑗𝜀 ′′ = 𝜀∞ + ; 0<𝛼<1
1 + (𝑗𝜔𝜏0 )𝛼
1 sinh 𝛼𝑥
𝑂ù 𝜀 ′ − 𝜀∞ = (𝜀𝑠 − 𝜀∞ ) (1 − )
2 1
cosh(𝛼𝑥) + cos ( (1 − 𝛼)𝜋)
2
1
1 cos 2 (1 − 𝛼)𝜋
′′
𝐸𝑡 𝜀 = (𝜀𝑠 − 𝜀∞ ) ( )
2 1
cosh(𝛼𝑥) + sin (2 (1 − 𝛼)𝜋)
𝑥 = ln(𝜔𝜏0 )
𝜏0 𝛼=1
𝜶
𝜶
∞
𝐺(𝜏)𝑑𝜏
𝜀(𝜔) − 𝜀𝑖𝑛𝑓 = (𝜀𝑠 − 𝜀∞ ) ∫
0 1 + 𝑗𝜔𝜏
1 sin(1−𝛼)𝜋
𝐹(𝑠) =
2𝜋 cosh 𝛼𝑠−cos(1−𝛼)𝜋
∞ ∞ 𝜏
∫0 𝐺(𝜏)𝑑𝜏 = ∫−∞ 𝐹(𝑠)𝑑𝑠 = 1 ; 𝑠 = ln( 𝜏0 )2
𝜏𝐺(𝜏) = 𝐹(𝑠)
𝛼 → 1, 𝐹(𝑠) ≡ 𝛿(𝜏 − 𝜏0 ) 𝛿
𝜏0
𝛼<1
𝜀 ′′ 𝜀′
𝜏
𝑠 = ln( )
𝜏0
1 (𝑗𝜔𝜏0 )𝛼
𝑌=
𝑅𝑒 1 + (𝑗𝜔𝜏0 )𝛼
1
𝑍=
𝑌
𝑅𝑒 𝜏0 = 𝑅𝑒 𝐶𝑒𝑓𝑓 𝐶𝑒𝑓𝑓
𝜏0
𝑄 = 𝑅𝑒 𝛼−1 𝐶𝑒𝑓𝑓 𝛼
1 1 𝑄(𝑗𝜔)𝛼
𝑌= = =
𝑍 𝑍𝐶𝑃𝐸 + 𝑅𝑒 1 + 𝑅𝑒 𝑄(𝑗𝜔)𝛼
1 1
𝑌 = 𝑅 (1 − 𝑅 𝑅
𝑒 1+ 𝑒 +(1+ 𝑒 )1−𝛼 (𝑗𝜔𝜏0 )𝛼
𝑅𝐶𝑇 𝑅𝐶𝑇
1 1
𝑌= (1 − )
𝑅𝑒 𝑅
1 + 𝑅 𝑒 + 𝑅𝑒 𝑄(𝑗𝜔)𝛼
𝑐𝑡
) 𝐶𝑒𝑓𝑓 𝛼
−1 1−𝛼
𝑄 = (𝑅𝑒−1 + 𝑅𝐶𝑇
𝑅𝐶𝑇
𝑃 = 𝑈𝐼cos(𝛼)
𝛼<1
 𝛼
𝛼 𝛼
𝛼
α
𝛼

𝑅𝐶𝑇

𝑓𝑐
𝑍0 − 𝑍∞
𝑍 = 𝑍∞ +
𝑓
1 + (𝑗 )1−𝑛
𝑓𝑐
𝑍0 𝑍∞
2𝜋
𝑓𝑐 = 𝜏0
1−𝑛=𝛼
𝐸2

𝐶𝑚𝑒𝑚

𝑹𝒃𝒖𝒍𝒌
𝑹𝒄𝒍 𝑪𝒄𝒍
𝑪𝒆𝒍
𝑄 𝛼




𝛼
𝛾
𝐶𝑂2
𝐶𝑂2 𝐶𝑂2
𝐶𝑂2
𝐻+ 𝐻𝐶𝑂3−
𝐶𝑂2 + 𝐻2 𝑂 = 𝐻2 𝐶𝑂3
𝐻2 𝐶𝑂3 = 𝐻 + + 𝐻𝐶𝑂3−







2. 1013 Ω
4.5 pF
𝜀𝑟
𝜀𝑟 𝜀0 𝑆
𝐶= ; 𝑒 = 1 𝑛𝑚, 𝜀𝑟 = 78.5, 𝜀0 = 8.85. 10−12 , 𝑆 = 𝜋𝑟²
𝑒
100 Ω
1 𝑘Ω

1/𝑓 𝑥

𝑄 𝛼 𝑅𝑒 𝑄
𝑅𝑒
𝑄 𝑅𝑒
𝑸 𝑹𝒆
1⁄
2𝜋𝑅𝐶
𝛼
𝜔 = 2𝜋𝑓
𝑅𝑄(𝑗𝜔)𝛼 + 1
𝑍=
𝑄(𝑗𝜔)𝛼
1
𝑍𝐶𝑃𝐸 =
𝑄(𝑗𝜔)𝛼
𝜋 𝜋
𝑅𝑄𝜔𝛼 𝑗𝑠𝑖𝑛(𝛼 2 ) + 𝑅𝑄𝜔𝛼 𝑐𝑜𝑠(𝛼 2 ) + 1
𝑍=
𝑄(𝑗𝜔)𝛼
𝜋
𝑅𝑄𝜔𝑐𝛼 𝑠𝑖𝑛(𝛼 2 ) 𝜋 𝜋
−1
tan ( 𝜋 ) − 𝛼 = −𝛼
𝑅𝑄𝜔𝑐𝛼 𝑐𝑜𝑠(𝛼 2 ) + 1 2 4
𝜋
tan 4 𝛼 1
𝜔𝑐 = ( 𝜋 𝜋 𝜋 )
𝛼
𝑅𝑄𝑠𝑖𝑛 (𝛼 2 ) − 𝑅𝑄𝑐𝑜𝑠 (𝛼 2 ) tan 4 𝛼
1
𝐾𝛼


80
𝛼= = 0.89
90
1
90.89 = 11.81




𝑹𝒎𝒆 𝑹𝒋𝒖𝒏𝒄
𝑹𝒆𝒙𝒕
𝑹𝒊𝒏𝒕

𝑅𝑖𝑛𝑡 𝑅𝑒𝑥𝑡
𝑅𝑖𝑛𝑡 𝑅𝑒𝑥𝑡
𝑅𝑒𝑥𝑡 𝑅𝑚𝑒𝑑
𝑅𝑚𝑒𝑑 = 𝑅𝑒𝑥𝑡 .
𝑅𝑒
𝑅𝑗𝑢𝑛𝑐
𝑹𝒎𝒆𝒅
Ω. 𝑚𝑚²
𝑅𝑐𝑒𝑙𝑙
1 𝑅𝑐𝑒𝑙𝑙
𝑍= + + 𝑅𝑚𝑒𝑑
𝑄𝑒𝑙𝑒𝑐𝑡𝑟𝑜𝑑𝑒 (𝑗𝜔) 𝑒𝑙𝑒𝑐𝑡𝑟𝑜𝑑𝑒 1 + 𝑅𝑐𝑒𝑙𝑙 𝑄𝑐𝑒𝑙𝑙 (𝑗𝜔)𝛼𝑐𝑒𝑙𝑙
𝛼
𝒁𝒑𝒂𝒓
𝑹𝒑𝒂𝒓 = 𝟏𝟎 𝑴𝛀, 𝑪𝒑𝒂𝒓 = 𝟐𝟐 𝒑𝑭 𝑳𝒑𝒂𝒓 = 𝟏𝟎 µ𝑯



1⁄
𝑄𝑐𝑒𝑙𝑙
1⁄ 𝑅𝑐𝑒𝑙𝑙
𝟏⁄
𝑸𝒄𝒆𝒍𝒍
𝟏⁄
 𝛼𝑐𝑒𝑙𝑙
𝛼𝑐𝑒𝑙𝑙
𝛼é𝑙𝑒𝑐𝑡𝑟𝑜𝑑𝑒

𝑅𝑐𝑡
𝑅𝑒
𝑄 𝛼
𝑄 𝛼
𝑄 𝛼
1 1
𝑌= (1 − −1 )
𝑅𝑒 1 + 𝑅𝑒 𝑅𝑐𝑡 + 𝑅𝑒 𝑄(𝑖𝜔)𝛼
𝜔𝑌′′𝑚𝑎𝑥 = (𝑄 −1 (𝑅𝑒−1 + 𝑅𝑐𝑡

−1 1/𝛼
))
𝑄 = 𝐶𝑒𝑓𝑓 𝛼=1
−1
𝐶𝑒𝑓𝑓 = 𝜔𝑌′′𝑚𝑎𝑥 (𝑅𝑒−1 + 𝑅𝑐𝑡
−1
)
1
𝛼= [log(𝑅𝑒−1 + 𝑅𝑐𝑡
−1 )
− log 𝑄]
log 𝜔𝑌′′𝑚𝑎𝑥
1
𝛼= −1 [log(𝑅𝑒−1 + 𝑅𝑐𝑡
−1 )
− log 𝑄]
log[𝐶𝑒𝑓𝑓 (𝑅𝑒−1 −1
+ 𝑅𝑐𝑡 )]
𝐶𝑒𝑓𝑓 𝑅𝑒 𝑅𝑐𝑡
𝐶𝑒𝑓𝑓 𝑅𝑒
𝑅𝑐𝑡
𝜶
𝑅𝑒 𝑅𝑐𝑡
𝑅𝑒 = 𝑅𝑚𝑒𝑑 𝑅𝑐𝑡 = 𝑅𝑐𝑒𝑙𝑙
𝜔𝑌′′𝑚𝑎𝑥 𝐶𝑒𝑓𝑓
1
𝑌= 𝑍
1⁄ 𝑄𝑐𝑒𝑙𝑙
1 −1 −1
1
𝛼𝑐𝑒𝑙𝑙 = [log(𝑅𝑚𝑒𝑑 + 𝑅𝑐𝑒𝑙𝑙 ) + log ]
log 𝜔𝑌′′𝑚𝑎𝑥 𝑄𝑐𝑒𝑙𝑙
𝜶𝒄𝒆𝒍𝒍 𝟏⁄
𝜶𝒄𝒆𝒍𝒍
𝛼𝑐𝑒𝑙𝑙 1⁄
𝐶𝑒𝑓𝑓
𝑄𝑐𝑒𝑙𝑙 𝛼𝑐𝑒𝑙𝑙 𝑅𝑐𝑒𝑙𝑙 𝑅𝑚𝑒𝑑
1 𝛼−1
𝛼 −1 −1
𝐶𝑒𝑓𝑓 = 𝑄𝑐𝑒𝑙𝑙 (𝑅𝑚𝑒𝑑 + 𝑅𝑐𝑒𝑙𝑙 )𝛼
1⁄
𝐶𝑒𝑓𝑓
𝟏⁄
𝑪𝒆𝒇𝒇
1⁄
1⁄
𝐶𝑒𝑓𝑓
1⁄

1⁄
𝐶𝑒𝑓𝑓
𝟏⁄
𝑪𝒆𝒇𝒇
1⁄ 𝛼
1⁄
1⁄
𝐶𝑒𝑓𝑓
1⁄ 𝛼 𝑅𝑐𝑒𝑙𝑙 1⁄
𝑄𝑐𝑒𝑙𝑙 𝐶𝑒𝑓𝑓

𝑅𝑐𝑒𝑙𝑙 𝛼𝑐𝑒𝑙𝑙
1⁄
𝟏⁄ 𝜶𝒄𝒆𝒍𝒍
𝑹𝒄𝒆𝒍𝒍
1⁄
𝐶𝑒𝑓𝑓
1⁄
𝐶𝑒𝑓𝑓
1⁄
1⁄
1⁄
𝟏⁄
𝑪𝒆𝒇𝒇

1⁄ 𝛼𝑐𝑒𝑙𝑙
1⁄
𝐶𝑒𝑓𝑓
1⁄

𝑌 4−
𝑌 4− + 𝐶𝑎2+ = 𝐶𝑎𝑌 2−
𝐾𝑓,𝑝𝐻=7 = 𝛼𝑌 4−,𝑝𝐻=7 𝐾𝑓,𝑝𝐻>13 𝛼𝑌 4−,𝑝𝐻=7 = 4.8 10−4
𝐾𝑓,𝑝𝐻>13 𝐾𝑓,𝐶𝑎2+,𝑝𝐻=7 =
2.6 107 𝐾𝑓,𝑀𝑔2+,𝑝𝐻=7 = 3.0 105



𝑹𝒄𝒆𝒍𝒍 𝟏⁄
1⁄
1 𝑅𝑐𝑒𝑙𝑙
𝑍= + + 𝑅𝑚𝑒𝑑
𝑄𝑒𝑙𝑒𝑐𝑡𝑟𝑜𝑑𝑒 (𝑗𝜔) 𝑒𝑙𝑒𝑐𝑡𝑟𝑜𝑑𝑒 1 + 𝑅𝑐𝑒𝑙𝑙 𝑄𝑐𝑒𝑙𝑙 (𝑗𝜔)𝛼𝑐𝑒𝑙𝑙
𝛼
1⁄
1⁄
1⁄
 1⁄
𝐶𝑒𝑓𝑓
1⁄
𝐶𝑒𝑓𝑓
1⁄
1⁄ 1⁄
1⁄
𝑪𝒆𝒇𝒇
𝑪𝒆𝒇𝒇
𝑪𝒆𝒇𝒇
𝑪𝒆𝒇𝒇
𝑅𝑐𝑒𝑙𝑙 1⁄
(𝑯𝟐 𝑶𝟐
(𝐻2 𝑂2
𝐻2 𝑂2
𝐻2 𝑂2
𝐻2 𝑂2
𝐻2 𝑂2
𝐻2 𝑂2
𝐻2 𝑂2
𝐻2 𝑂2
𝐻2 𝑂2
𝐻2 𝑂2 1⁄
𝐻2 𝑂2
𝑯𝟐 𝑶𝟐
𝜶𝒄𝒆𝒍𝒍 𝟏⁄
𝑯𝟐 𝑶𝟐
𝑯𝟐 𝑶𝟐
𝑅𝑐𝑒𝑙𝑙 1⁄ 1⁄
𝐶𝑒𝑓𝑓 𝑄𝑐𝑒𝑙𝑙
𝐶𝑒𝑓𝑓
𝐻2 𝑂2
1⁄
𝐻2 𝑂2
𝑪𝒆𝒇𝒇
𝑯𝟐 𝑶𝟐
𝐻2 𝑂2
1⁄
1⁄
1⁄
𝐻2 𝑂2
𝑹𝟎∎
𝟐
𝐻2 𝑂2
𝑂𝐻 ∎ 𝐻2 𝑂2
𝐹𝑒 2+ + 𝐻2 𝑂2 = 𝐹𝑒 3+ + 𝑂𝐻 − + 𝑂𝐻 ∎
𝐹𝑒 3+ + 𝐻2 𝑂2 = 𝐹𝑒 2+ + 𝐻 + + 𝐻𝑂2∎
(k =
0.01 M −1 s−1 contre 51 M −1 s −1 )
1038
𝟏⁄
𝑪𝒆𝒇𝒇




𝑅𝑐𝑒𝑙𝑙 1⁄ 𝑅𝑐𝑒𝑙𝑙
1⁄
𝐶𝑒𝑓𝑓 .
𝟏⁄ 𝑹𝒄𝒆𝒍𝒍
𝟏⁄
𝑪𝒆𝒇𝒇
𝑪𝒆𝒇𝒇

𝐶𝑒𝑓𝑓
1⁄ 1⁄
1⁄
1⁄
Vcm−1


Vcm−1
𝟏⁄
1⁄

𝑪𝒆𝒇𝒇
𝑪𝒆𝒇𝒇
1⁄
𝐶𝑒𝑓𝑓
1⁄
𝐶𝑒𝑓𝑓
1⁄
𝐶𝑒𝑓𝑓
1⁄
𝐶𝑒𝑓𝑓

𝑪𝒆𝒇𝒇
1⁄
1⁄ 𝛼𝑐𝑒𝑙𝑙 𝑅𝑐𝑒𝑙𝑙
1⁄
𝑄𝑐𝑒𝑙𝑙 , 𝛼𝑐𝑒𝑙𝑙
1⁄
𝐶𝑒𝑓𝑓
1⁄
1⁄
𝐻2 𝑂2


1
𝑓=
𝛿
𝑇0 𝑃0
𝐸 𝐸 𝑒𝑞
𝐸 𝑒𝑞 𝐸 𝑒𝑞
𝐸 𝑒𝑞 = 𝐸 0
−𝐹(𝐸 − 𝐸 0 )
−(Δ𝐺𝑎,𝑟𝑒𝑑 +𝛾𝐹(𝐸−𝐸 0 ))⁄
𝑗𝑟𝑒𝑑 (𝐸) = 𝐴𝑟𝑒𝑑 𝐶𝑟𝑒𝑑 (𝑥 = 0, 𝑡)𝑒 𝑅𝑇
−(Δ𝐺𝑎,𝑜𝑥 −(1−𝛾)𝐹(𝐸−𝐸 0 ))⁄

𝑗𝑜𝑥 (𝐸) = 𝐴𝑜𝑥 𝐶𝑜𝑥 (𝑥 = 0, 𝑡)𝑒 𝑅𝑇
𝑗𝑡𝑜𝑡 (𝐸) = 𝑗𝑟𝑒𝑑 (𝐸) − 𝑗𝑜𝑥 (𝐸)
𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥 = 0, 𝑡)
Δ𝐺𝑎,𝑜𝑥/𝑟𝑒𝑑
𝛾 0<𝛾<1
𝐸 = 𝐸0
−Δ𝐺𝑎,𝑟𝑒𝑑 −Δ𝐺𝑎,𝑜𝑥
⁄𝑅𝑇 ⁄𝑅𝑇
𝐴𝑟𝑒𝑑 𝑒 = 𝐴𝑜𝑥 𝑒 = 𝑘0
𝛼
𝛾
−𝛾𝐹(𝐸−𝐸 0 )⁄ (1−𝛾)𝐹(𝐸−𝐸 0 )
⁄
𝑖(𝐸) = −𝑛𝑆𝐹𝑘 0 (𝐶𝑟𝑒𝑑 (𝑥 = 0, 𝑡)𝑒 𝑅𝑇 − 𝐶𝑜𝑥 (𝑥 = 0, 𝑡)𝑒 𝑅𝑇 )
1 𝜕𝑖
= (𝐸)
𝑅𝐶𝑇 (𝐸) 𝜕𝐸
𝑛2 𝑆𝐹²𝑘 0 −𝛾𝐹(𝐸−𝐸 0 )⁄ (1−𝛾)𝐹(𝐸−𝐸 0 )
⁄
= (𝛾𝐶𝑟𝑒𝑑 (𝑥 = 0, 𝑡)𝑒 𝑅𝑇 + (1 − 𝛾)𝐶𝑜𝑥 (𝑥 = 0, 𝑡)𝑒 𝑅𝑇 )
𝑅𝑇
2.5. 10−8 𝑚/𝑠
𝐻 + /𝐻2 1. 10−14 𝑚/𝑠 𝑂2 /𝐻2 𝑂
10−7 Ω−1 10−13 Ω−1
𝐸 − 𝐸0
𝑅𝐶𝑇 (𝐸~𝐸 0 )
𝑅𝐶𝑇 (𝐸, 𝑡 = 0)
𝑅𝐶𝑇 (𝐸)
𝜕𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥, 𝑡) 𝜕²𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥, 𝑡)

= 𝐷𝑜𝑥/𝑟𝑒𝑑
𝜕𝑡 𝜕𝑥²
𝐷𝑜𝑥/𝑟𝑒𝑑
𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥, 𝑡) = 𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (𝑥, 𝑡) + ∆𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥)𝑒 𝑗𝜔𝑡

𝛿𝑁
𝛿𝑁 𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠
𝑥
𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (𝑥) = 𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (0) + (𝐶𝑜𝑥/𝑟𝑒𝑑,𝑒𝑙 − 𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (0))
𝛿𝑁
𝑝𝑜𝑢𝑟 𝑥 < 𝛿𝑁 ; 𝐶𝑜𝑥/𝑟𝑒𝑑,𝑒𝑙 𝑝𝑜𝑢𝑟 𝑥 ≥ 𝛿𝑁 (𝑑𝑎𝑛𝑠 𝑙 ′ é𝑙𝑒𝑐𝑡𝑟𝑜𝑙𝑦𝑡𝑒)
𝑒 𝑗𝜔𝑡
𝑗𝜔
−𝛿𝑁 √
𝐷𝑜𝑥/𝑟𝑒𝑑 𝑗𝜔
∆𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥) = −2𝑁𝑜𝑥/𝑟𝑒𝑑 𝑒 sinh((𝑥 − 𝛿𝑁 )√ )
𝐷𝑜𝑥/𝑟𝑒𝑑
𝑝𝑜𝑢𝑟 𝑥 < 𝛿𝑁 ; 0 𝑝𝑜𝑢𝑟 𝑥 ≥ 𝛿𝑁
∆𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥 = 𝛿𝑁 ) = 0
𝛿𝑁
∆𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥 = ∞) = 0
𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (𝑥) = 𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (0)
𝑗𝜔 𝑗𝜔
−𝑥√ −𝑥√
𝐷𝑜𝑥/𝑟𝑒𝑑 𝐷𝑜𝑥/𝑟𝑒𝑑
∆𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥) = 𝑁𝑜𝑥/𝑟𝑒𝑑 𝑒 = ∆𝐶𝑜𝑥/𝑟𝑒𝑑 (0)𝑒
𝑒 𝑗𝜔𝑡
𝜕𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥, 𝑡) 𝑗𝜔
𝑖𝐷𝑜𝑥/𝑟𝑒𝑑 = −𝑛𝐹𝑆𝐷𝑜𝑥/𝑟𝑒𝑑 (𝑥 = 0) = 𝑛𝐹𝑆𝐷𝑜𝑥/𝑟𝑒𝑑 √ ∆𝐶 (0)
𝜕𝑥 𝐷𝑜𝑥/𝑟𝑒𝑑 𝑜𝑥/𝑟𝑒𝑑
𝑖𝐷𝑜𝑥/𝑟𝑒𝑑 = 𝑖
𝜕𝑖 𝜕𝑖 𝜕𝑖 𝜕𝑖 𝜕𝑖
𝛿𝑖 = 𝛿𝐸 + 𝛿𝐶 = 𝛿𝑖 = 𝛿𝐸 + 𝛿𝐶𝑟𝑒𝑑 − 𝛿𝐶
𝜕𝐸 𝜕𝐶 𝜕𝐸 𝜕𝐶𝑟𝑒𝑑 𝜕𝐶𝑜𝑥 𝑜𝑥
1 −𝛾𝐹(𝐸−𝐸 0 )⁄ (1−𝛾)𝐹(𝐸−𝐸 0 )
⁄
𝛿𝑖 = 𝛿𝐸 − 𝑛𝑆𝐹𝑘 0 (𝑒 𝑅𝑇 + 𝑒 𝑅𝑇 )𝛿𝐶
𝑅𝐶𝑇
𝛿𝐶 = ∆𝐶𝑜𝑥/𝑟𝑒𝑑 (0)
1 −𝛾𝐹(𝐸−𝐸 0 )⁄ (1−𝛾)𝐹(𝐸−𝐸 0 )
⁄ 𝛿𝑖
𝛿𝑖 = 𝛿𝐸 − 𝑘 0 (𝑒 𝑅𝑇 + 𝑒 𝑅𝑇 )
𝑅𝐶𝑇 √𝑗𝜔𝐷
𝛿𝐸
𝛿𝑖
−𝛾𝐹(𝐸−𝐸 0 )⁄ (1−𝛾)𝐹(𝐸−𝐸 0 )
⁄
𝑘 0 (𝑒 𝑅𝑇 +𝑒 𝑅𝑇 )
𝑍𝐶𝑇 = 𝑅𝐶𝑇 1 + = 𝑅𝐶𝑇 + 𝑊

√𝑗𝜔𝐷
( )
1/√𝑗𝜔 = (𝑗𝜔)−1/2
1⁄
𝐶𝑒𝑓𝑓
1⁄
𝑄𝑐𝑒𝑙𝑙 , 𝛼𝑐𝑒𝑙𝑙
1⁄
𝑄𝑐𝑒𝑙𝑙 𝛼𝑐𝑒𝑙𝑙
1⁄
𝑄𝑐𝑒𝑙𝑙 𝛼𝑐𝑒𝑙𝑙
𝑅𝑐𝑒𝑙𝑙 1⁄ 1⁄
𝑄𝑐𝑒𝑙𝑙 𝛼𝑐𝑒𝑙𝑙 𝐶𝑒𝑓𝑓
α

TH2019PESC2044

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

TH2019PESC2044

Uploaded by

Copyright:

Available Formats

Contribution à la modélisation d’interface biologique par

spectroscopie d’impédance : application au suivi de

To cite this version:

HAL Id: tel-02482728

HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est

10−2 𝐹/𝑚², 𝑔𝑚𝑒𝑚𝑏 = 5 𝑆/𝑚² 𝑒𝑡 𝜌 = 1 Ω𝑚

𝑉𝐸𝑇/𝑅𝐸 = 𝑉𝐸𝑇/𝑀 − 𝑉𝑅𝐸/𝑀 = 𝑉𝑒 .

𝑉𝐶𝐸/𝑀 = −𝑉𝑒 𝑉𝐸𝑇/𝑀 = 0

e = 1 nm, 𝜀0 = 8.85. 10−12 𝐹/𝑚 𝜀 = 78.5

𝜔𝑌′′𝑚𝑎𝑥 = (𝑄 −1 (𝑅𝑒−1 + 𝑅𝑐𝑡

𝑅𝑒 = 𝑅𝑚𝑒𝑑 𝑅𝑐𝑡 = 𝑅𝑐𝑒𝑙𝑙

𝑄𝑐𝑒𝑙𝑙 𝛼𝑐𝑒𝑙𝑙 𝑅𝑐𝑒𝑙𝑙 𝑅𝑚𝑒𝑑

𝐾𝑓,𝑝𝐻=7 = 𝛼𝑌 4−,𝑝𝐻=7 𝐾𝑓,𝑝𝐻>13 𝛼𝑌 4−,𝑝𝐻=7 = 4.8 10−4

2.6 107 𝐾𝑓,𝑀𝑔2+,𝑝𝐻=7 = 3.0 105

0.01 M −1 s−1 contre 51 M −1 s −1 )

−(Δ𝐺𝑎,𝑜𝑥 −(1−𝛾)𝐹(𝐸−𝐸 0 ))⁄

𝑗𝑡𝑜𝑡 (𝐸) = 𝑗𝑟𝑒𝑑 (𝐸) − 𝑗𝑜𝑥 (𝐸)

2.5. 10−8 𝑚/𝑠

𝐻 + /𝐻2 1. 10−14 𝑚/𝑠 𝑂2 /𝐻2 𝑂

10−7 Ω−1 10−13 Ω−1

𝜕𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥, 𝑡) 𝜕²𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥, 𝑡)

𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥, 𝑡) = 𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (𝑥, 𝑡) + ∆𝐶𝑜𝑥/𝑟𝑒𝑑 (𝑥)𝑒 𝑗𝜔𝑡

𝑝𝑜𝑢𝑟 𝑥 < 𝛿𝑁 ; 0 𝑝𝑜𝑢𝑟 𝑥 ≥ 𝛿𝑁

𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (𝑥) = 𝐶𝑜𝑥/𝑟𝑒𝑑,𝑏𝑖𝑎𝑖𝑠 (0)

𝑍𝐶𝑇 = 𝑅𝐶𝑇 1 + = 𝑅𝐶𝑇 + 𝑊

You might also like