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(Download PDF) Microbiology Principles and Explorations 7th Edition Black Test Bank Full Chapter
(Download PDF) Microbiology Principles and Explorations 7th Edition Black Test Bank Full Chapter
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Import Settings:
Base Settings: Brownstone Default
Information Field: Difficulty
Highest Answer Letter: D
Multiple Keywords in Same Paragraph: No
Multiple Choice
1. A linear sequence of DNA nucleotides which provides the genetic information for a single
characteristic is a
A) chromosome
B) plasmid
C) gene
D) base pair
Ans: C
Difficulty: Easy
Feedback: 7.1
Ans: D
Difficulty: Easy
Feedback: 7.1
3. A plasmid is a/an:
A) extrachromosomal piece of DNA
B) chromosomal site to which genetic activity can be traced
C) body found in the cytoplasm that directs protein synthesis
D) molecule that carries the genetic message of the chromosomal DNA
Ans: A
Difficulty: Easy
Feedback: 7.1
Ans: A
Difficulty: Easy
Feedback: 7.1
Ans: C
Difficulty: Easy
Feedback: 7.1
6. In DNA, the base adenine always pairs with what other base?
A) guanine
B) adenine
C) uracil
D) thymine
Ans: D
Difficulty: Medium
Feedback: 7.1
Ans: A
Difficulty: Medium
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Ans: B
Difficulty: Medium
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Ans: C
Difficulty: Easy
Feedback: 7.1
10. Reverse transcription occurs when
A) RNA is used as a template to produce DNA
B) DNA is used as a template to produce RNA
C) Protein is used as a template to produce RNA
D) RNA is used as a template to produce protein
Ans: A
Difficulty: Medium
Feedback: 7.1
Ans: B
Difficulty: Easy
Feedback: 7.1
Ans: C
Difficulty: Medium
Feedback: 7.1
13. Mutations
A) when occurring on a particular Okazaki fragment can lead to different loci
B) often change a person's blood from type A to type AB
C) are responsible for heritable variations seen in progeny
D) occur only when the DNA is transmitted to a daughter cell
Ans: C
Difficulty: Hard
Feedback: 7.1
Ans: C
Difficulty: Medium
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Ans: A
Difficulty: Medium
Feedback: 7.1
Ans: A
Difficulty: Medium
Feedback: 7.2
Ans: C
Difficulty: Medium
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Ans: C
Difficulty: Medium
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19. After DNA replication of a bacterial chromosome, the new DNA contains
A) 2 newly synthesized strands of DNA copied from the original parent strands
B) one strand of parent DNA along with one newly synthesized strand
C) small segments of parent DNA interspersed with newly synthesized segments of DNA
D) two of the above
Ans: B
Difficulty: Medium
Feedback: 7.2
Ans: C
Difficulty: Easy
Feedback: 7.3
Ans: C
Difficulty: Medium
Feedback: 7.3
Ans: A
Difficulty: Medium
Feedback: 7.3
24. The information carried by DNA is used to create a ________ which then moves to the
ribosome for protein synthesis.
A) tRNA
B) rRNA
C) siRNA
D) mRNA
Ans: D
Difficulty: Medium
Feedback: 7.3
25. A mRNA molecule can be broken down into three nucleotide units called
A) amino acids
B) bases
C) codons
D) anticodons
Ans: C
Difficulty: Medium
Feedback: 7.3
26. Amino acids move from the cytoplasm to the ribosome with the help of
A) mRNA's
B) tRNA's
C) siRNA's
D) rRNA's
Ans: B
Difficulty: Medium
Feedback: 7.3
Ans: C
Difficulty: Medium
Feedback: 7.3
29. Which of the following statements about the genetic code is true?
A) the first position of a codon determines the amino acid
B) an amino acid can be specified for by more than one codon
C) nonsense codons contain no information
D) because DNA only contains three letters there are very few possible genes that can exist
Ans: B
Difficulty: Hard
Feedback: 7.3
Ans: D
Difficulty: Hard
Feedback: 7.3
31. In eukaryotes, transcription takes place in the ________ while translation takes place in the
________.
A) nucleus, nucleus
B) nucleus, cytoplasm
C) cytoplasm, nucleus
D) cytoplasm, cytoplasm
Ans: B
Difficulty: Medium
Feedback: 7.3
Ans: B
Difficulty: Easy
Feedback: 7.3
Ans: C
Difficulty: Easy
Feedback: 7.3
Ans: B
Difficulty: Medium
Feedback: 7.4
Ans: C
Difficulty: Easy
Feedback: 7.4
37. When a cell contains high levels of lactose, the lactose will bind with the
A) repressor
B) operator
C) promoter
D) RNA polymerase
Ans: A
Difficulty: Medium
Feedback: 7.4
38. In the lactose operon, which of the following is a protein?
A) repressor
B) operator
C) regulator
D) promoter
Ans: A
Difficulty: Hard
Feedback: 7.4
39. With regard to the lactose operon, which of the following is false under conditions of low
(or no) lactose?
A) the RNA polymerase cannot transcribe structural genes
B) the repressor is bound to the operator
C) lactose is not bound to the repressor
D) RNA polymerase can bind to the promoter
Ans: D
Difficulty: Hard
Feedback: 7.4
Ans: A
Difficulty: Easy
Feedback: 7.4
41. The tryptophan (trp) operon is turned on (i.e. gene expression occurs) when
A) there is a great deal of tryptophan in the cell
B) there is a lack of tryptophan in the cell
C) the trp operon is always turned on
D) the trp operon is always turned off
Ans: B
Difficulty: Hard
Feedback: 7.4
Ans: C
Difficulty: Easy
Feedback: 7.5
Ans: D
Difficulty: Hard
Feedback: 7.4
Ans: A
Difficulty: Medium
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45. A DNA mutation that results in no change in protein product produced is termed a
A) missense mutation
B) nonsense mutation
C) silent mutation
D) frameshift mutation
Ans: C
Difficulty: Medium
Feedback: 7.5
46. Organisms that have lost the ability to synthesize a particular enzyme and which require
certain nutrients to be added to their medium to maintain growth are termed
A) phototrophs
B) auxotrophs
C) prototrophs
D) autotrophs
Ans: B
Difficulty: Hard
Feedback: 7.5
Ans: C
Difficulty: Medium
Feedback: 7.5
Ans: C
Difficulty: Hard
Feedback: 7.5
50. Photoreactivation
A) repairs dimers in DNA using an endonuclease
B) uses light to activate repair enzymes
C) removes alkylating agents from bases
D) occurs when UV light causes mutations in DNA
Ans: B
Difficulty: Medium
Feedback: 7.5
51. The fluctuation tests done by Luria and Delbruck showed that
A) antibiotics induce the development of resistance in bacteria
B) the growth of bacteria fluctuates based on the concentration of antibiotics in the media
C) the concentration of antibiotics fluctuates in response to the number of bacteria in a sample
D) resistance to antibiotics occurs spontaneously in bacteria
Ans: D
Difficulty: Hard
Feedback: 7.5
52. The Ames test is used to determine if a chemical
A) increases the rate at which a bacterial cell divides
B) decreases the number of cells in a culture
C) induces mutations in a cell's DNA
D) decreases the ability of a cell to photosynthesize
Ans: C
Difficulty: Medium
Feedback: 7.5
Ans: C
Difficulty: Medium
Feedback: 7.5
Ans: D
Difficulty: Hard
Feedback: 7.5
Essay
55. RNA plays several important roles in the processes that translate genetic information from
DNA into protein products. Illustrate the events of transcription in a bacterial cell and indicate
where RNA plays a role. Also indicate what role RNA plays in translation in bacterial cells.
Ans: The following should be illustrated: Transcription transfers the genetic information from
DNA to mRNA. It occurs with RNA polymerase (protein) binding to a DNA strand and pairing
RNA nucleosides with the DNA. The binding RNA nucleosides are made into a chain termed
mRNA and synthesized in the 3' to 5' direction of the DNA molecule. RNA plays a role in
transcription as the end product is mRNA.
During translation RNA acts as the source of genetic information to construct the proteins in the
form of mRNA. In addition RNA forms vital portions of ribosomes, which are the protein
synthesis complexes and brings the amino acids to the ribosomes and matches the codons to
amino acids in the form of tRNA.
Difficulty: Easy
Feedback: 7.3
56. Using the lac and trp operons as examples, describe the two control mechanisms for protein
synthesis: one based on enzyme induction and the other based on enzyme repression.
Ans: In the case of the lac operon, when the inducer substance (lactose) is absent, a repressor
protein binds to the operator preventing transcription of the gene coding for enzymes used to
metabolize lactose. When the inducer substance is present it binds to the repressor and
inactivates it, allowing for the transcription of the genes responsible for metabolizing lactose
(structural genes).
Enzyme repression occurs when an adequate supply of the products of enzyme activity leads to
repressing the transcription of the operon. In the trp operon, when tryptophan is available it
binds to an inactive repressor which activates a repressor protein that represses the synthesis of
the enzymes needed to metabolize lactose.
Difficulty: Medium
Feedback: 7.4
57. Microbial evolutionary biologists have provided evidence against Lamarckian evolution, by
demonstrating that genetic mutations arise in the absence of selection, rather than being a
response to selection. Describe one method for distinguishing between spontaneous and induced
mutations (be sure to include the expected results)
Ans: Spontaneous and induced mutations can be distinguished by either the fluctuation test or
replica plating. The fluctuation test involves inoculating a small number of bacteria into
separate cultures. If the mutation to acquire antibiotic resistance is spontaneous than the
replicate culture will have a highly variable number of mutants. If however the resistance is
induced by the presence of the antibiotic, all of the replicate cultures experiencing the antibiotic
should have the same number of mutants induced by the antibiotic. Replica plating occurs when
cultures are transferred from a master dish to a selective plate while maintaining the original
spatial pattern of colonies. Any colonies observed on a penicillin plate (selective) came from
bacteria that would have had resistance to penicillin without ever having been exposed to it
(spontaneous). If penicillin was inducing mutations then a constant fraction of bacteria
transferred should grow on the penicillin plate and a constant fraction of the colonies would be
present.
Difficulty: Medium
Feedback: 7.5
Another random document with
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further development; (3) a shell surrounding the germ cell and
vitelline cells, and provided at one end with a cap or operculum. The
eggs escape from the uterus of the adult through the vulva, are
carried to the intestine of the host with the bile, then pass through
the intestines with the contents of the latter, and are expelled from
the host with the fæcal matter. Many of them become dried, and then
undergo no further development; but others are naturally dropped in
the water in marshes, or, being dropped on dry ground, they are
washed into water by the rain, or are carried to a more favourable
position by the feet of animals pasturing or passing through the
fields. After a longer or shorter period of incubation, which varies
with the temperature, a ciliated embryo (miracidium) is developed.
At a temperature of 20° to 26° C. the miracidium may be formed in
ten days to three weeks; at a temperature of 16° C. the development
takes two to three months; at 38° C. it ceases entirely. Experiments
have shown that as long as these eggs remain in the dark the
miracidium will not escape from the egg-shell; accordingly it will not
escape during the night. When exposed to the light, however, or
when suddenly brought into contact with cold water, the organism
bursts the cap from the egg-shell, crawls through the opening, and
becomes a—
Fig. 148.—Embryo of the Fig. 149.—Sporocyst of the
common liver fluke common liver fluke which has
(Fasciola hepatica), developed from the embryo, and
boring into a snail. × 370. contains germinal cells. × 200.
(After Thomas, 1883, p. (After Leuckart, 1889, p. 109, Fig.
285, Fig. 4.) 67 B.)
Fig. 150.—Sporocyst of the
common liver fluke, somewhat
older than that of Fig. 149, in
which the germinal cells are
giving rise to rediæ. × 200.
(After Leuckart, 1889, p. 109,
Fig. 67 C.)
From the above we see that this parasite runs through three
generations, namely:
(1.) Ovum, miracidium, and sporocyst ... first generation.
(2.) Redia ... second generation.
(3.) Cercaria and adult ... third generation.
During this curious development, which lasts about ten to twelve
weeks, there is a constant potential increase in the number of
individuals, for each sporocyst may give rise to several (five to eight)
rediæ, each redia to a larger number (twelve to twenty) cercariæ, and
each adult to an enormous number (37,000 to 45,000) of eggs. This
unusual fertility of the parasite is necessary because of the
complicated life history and the comparatively small chance any one
egg has of completing the entire cycle.
Hosts. An interesting and, from an agricultural standpoint, an
important matter connected with this fluke is that it is found in a
large number (about twenty-five) of domesticated and wild animals,
and this fact probably explains to some degree the wide geographical
distribution of the parasite.