Grade 11 Forensic Science Core

Unit 6 Formative Test

DNA Profiling

Name: _____________________________________________

Section 1: Multiple choice questions

Circle or highlight the correct answer.

1 The main purpose of agarose gel electrophoresis is for:

A. DNA extraction
B. DNA fingerprinting
C. Separation of DNA fragments
D. Detection of DNA

2 DNA profiling is used in …

A. Forensic science
B. Paternal disputes
C. Research work
D. All of the above

3 A special protein that targets a specific base sequence and cuts DNA into smaller
fragments is a(n)

A. Gene
B. DNA Probe
C. Exon
D. Restriction Enzyme

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4 Gel electrophoresis is used to separate DNA fragments on the basis of which property?

A. Mass/size
B. Color
C. Functions
D. Chromosomes

5 Why do the fragments of DNA in gel electrophoresis travel away from the negative
electrode?

A. DNA is negatively charged so attracted to the positive end of the unit

B. DNA is positively charged to attracted to the negative end of the unit
C. the agarose gel in negatively charged
D. the agarose gel is positively charged

6 DNA stands for

A. DeoxyriboNucleic Acid
B. DunderNucleic Acid
C. DopamineNucleic Acid
D. DeciduousNucleic Acid

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7 Which DNA fragment bands move faster and further in a gel electrophoresis?

A. there is no way to tell

B. the largest ones
C. the smallest ones
D. none of these

Bob was found dead in his home. Crime scene analysis showed signs of a struggle.
Blood was collected from the crime scene, the victim, and three suspects. Which
suspect likely committed the crime?

A. Sue
B. Lisa
C. John
D. None of the above

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Which male is the father of this child?

A. Male 1
B. Male 2
C. Neither could be the father
D. Either could be the father

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Section 2: Structured questions
Question 1:
Mr. Chan’s family consists of mom, dad, and four kids. The parents have one daughter and one
son together, another daughter is from the mom’s previous marriage, and the other son is
adopted. Here are the DNA analysis results:

a. Which child is adopted? Why? (2)

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b. Which child is from the mom’s previous marriage? Why? (2)

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c. Who are the two biological children of Mr. and Mrs. Chan? (2)

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[6]

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Question 2:
Mr. Russ is investigating a murder scene. The felon was scratched by his victim and some of his
skin cells were found under the victim’s fingernails. A DNA test was performed.

a. Which of the suspects is the murderer? Why?

__________________________________________________________________

________________________________________________________________

[2]

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Question 3:

The following paper cup of coffee was found at a murder crime scene and
gathered as evidence.

a. Name the most likely bodily fluid that can be found on this coffee cup that can (1)
contain DNA.

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b. Tick the correct box below. The DNA evidence found on the coffee cup would be (1)
classified as:
Individual evidence
Class evidence

The DNA extracted from this piece of evidence will be prepared to undergo gel
electrophoresis. The set-up is shown below:

c. Label parts (i) and (ii) (2)

d. Will the DNA fragments with the greatest mass be at point A or B after the (1)
process?

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e. There’s one hidden mistake in this sketch… Identify it. (1)

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TOTAL: 23 MARKS