DNA PROFILING - UNIT REVISION WORKSHEET

First, review from your unit notes. Then, attempt to complete this worksheet to test your
knowledge.

Multiple choice questions

1. Which of the following best describes a DNA molecule?

A. double helix
B. contains ribose
C. contains Uracil
D. made of amino acids

2. Why is DNA important?

A. because we need it every day for energy

B. it serves as the blueprint for traits of all living things
C. it's in everything
D. it is very small and very complicated

3. Which sequence of DNA bases would pair with this partial strand ATG TGA CAG

A. ATG TGA CAG

B. TAC ACT GTC
C. CAT TCA CTG
D. GTA AGT GAC

4. Which of the following units are repeatedly joined together to form a strand of DNA?

A. fatty acids
B. amino acids
C. Nucleotides
D. polysaccharides

5. In a molecule of double-stranded DNA, the amount of Adenine present

is always equal to the amount of

A. Cytosine
B. Uracil
C. Guanine
D. thymine

6. Adenine bonds with ______________.

A. Cytosine
B. Adenine
C. Thymine
D. Guanine
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7. Guanine bonds with ______________.

A. Adenine
B. Thymine
C. Guanine
D. Cytosine

8. What determines the code, or information, of a DNA molecule?

A. the order (sequence) of the nitrogen bases

B. the shape (structure) of the nitrogen bases
C. the frequency (number) of nitrogen bases
D. the colour of the nitrogen bases

9. Which organelle is DNA usually found in?

A. Vacuole
B. cell membrane
C. Nucleus
D. chloroplast

10. Nitrogenous bases are joined by which type of bond?

A. hydrogen bonds
B. polar bonds
C. ionic bonds
D. covalent bonds

11. Replicate the following strand of DNA: AATCATGGA

A. TTAGTACCT
B. UUAGUACCU
C. AATCATGGA
D. GGATAUCUA

12. A process in which DNA has the unique ability to make an exact copy of itself.

A. Replication
B. Translation
C. Transcription

13. What is the correct complimentary strand for the following DNA? ATCGAG

A. TAGCTC
B. TACGAG
C. TAGCTG
D. ATCGAG

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14. The drawing shows the structure of what molecule?

A. A carbohydrate molecule
B. A lipid molecule
C. A protein molecule
D. A DNA molecule

15. What are the 4 nitrogen bases?

A. adenine, thymine cytosine, and guanine

B. adenine, thymine, cytosine, and glycerol
C. adenine, thymine, cytosine, and glucose
D. adenine, thymine, cytoplasm, and guanine

16. What are the three components of a nucleotide?

A. sugar, phosphate, nitrogen base

B. sugar, oxygen, nitrogen base
C. sugar, phosphate, protein
D. sugar, hydrogen, nitrogen base

17. The process of DNA Fingerprinting is based on the fact that

A. everyone has the exact same DNA.

B. no 2 people, except identical twins, have exactly the same DNA as someone else.
C. most genes are dominant.
D. None of the other answer choices are correct.

18. (GAAT) (GAAT) (GAAT) (GAAT) is an example of:

a. restriction fragments.
b. DNA fingerprinting.
c. PCR
d. STR

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19. The total amount of DNA in a cell, which is contained in the cell's nucleus (nuclear DNA) and
mitochondria (mtDNA), is called the human:

A. Genome.
B. Gene
C. Allele
D. RNA.

20. Which method is used to identify different STR markers on the agarose gel after it has been
separated by means of electrophoresis?

A. x-raying DNA samples

B. adding fluorescent dyes to make them visible
C. calculating the allele frequency

21. What material is used to isolate and cut DNA?

A. separating enzymes
B. restriction enzymes
C. STR
D. base pairs

22. Name a method of separating molecules, such as DNA, based on their size

A. Electrophoresis
B. restriction enzymes
C. STR

23. If guanine makes up 19% of the nucleotides in a sample of DNA from an organism, then
cytosine would make up _____ percent of the bases.

A. 19%
B. 62%
C. 31%

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24. Based on these results, whose blood was found in the blood stain at the crime scene?

A. Bob
B. Sue
C. John
D. Lisa

25. Which two bear species are most closely related?

A. Bear 1 and Bear 2

B. Bear 1 and Bear 3
C. Bear 2 and Bear 3
D. There’s no way to tell

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26. Which man is the father of this baby?

A. Father 1
B. Father 2
C. Father 3
D. None of them

27. Why do the fragments of DNA in gel electrophoresis travel away from the negative
electrode?

A. DNA is negatively charged so attracted to the positive end of the unit

B. DNA is positively charged to attracted to the negative end of the unit
C. the agarose gel is negatively charged
D. the agarose gel is positively charged

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Structured questions

Question 1:

Mr. Jones was killed in a car accident. Afterwards, a former girlfriend filed a paternity suit
claiming that Mr. Jones was the father of her child, and thus she was entitled to the man’s
estate. Which sample of DNA would have to be from Mr. Jones if he were the child’s father? (1)

Question 2:

Compare the profiles of three men with that of a mother and child to determine the biological
father.

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Question 3:
The following image is a set-up of gel electrophoresis.

Label the following parts on the image using letters. Use a ruler to draw your lines!

A. Positive anode
B. Wells
C. Agarose gel
D. DNA bands that has been separated