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Yakeen 2.0 2023

Biotechnology : Principles and Processes DPP-01

1. A limitation associated with traditional hybridisation 6. Hybridoma technology has been successfully used in
procedures used in plant and animal breeding (1) Production of somatic hybrids
overcome by genetic engineering is (2) Synthesis of monoclonal antibodies
(1) Traditional hybridisation is difficult to practice (3) Synthesis of haemoglobin
(2) Traditional hybridisation is labour and cost (4) Production of alcohol in bulk
intensive
(3) Traditional hybridisation may lead to inclusion 7. Which of the following is a biotechnological process?
and multiplication of non desirable genes (1) IVF leading to ‘test tube baby’
(4) The progeny generated by traditional (2) Developing a DNA vaccine
hybridisation have lower reproductive potential (3) Synthesising a gene and using it
(4) All of the above
2. The scientists who cloned DNA from the Salmonella
typhimurium streptomycin resistance plasmid into 8. Which of the following organelles is associated with
the Escherichia coli plasmid, observed tolerance to genetic engineering?
streptomycin among the transformants and thus (1) Plasmids (2) Plastids
laying the foundations of genetic engineering were (3) Chloroplast (4) Mitochondria
(1) Cohen and Boyer
(2) Millstein and Kohler 9. Which of the following is the most accepted definition of
(3) Bolivar and Rodriguez biotechnology by European Federation of
(4) Fire and Mello Biotechnology?
(1) Maintenance of sterile ambience for enabling
3. Production of transgenic plants is easier than
growth of desired microbe/eukaryotic cell in large
production of transgenic animals mainly because
quantities
(1) Plant cells can grow in cell culture
(2) Technique of using live organism or enzyme from
(2) Plant cells have a lower number of potentially
organisms to produce products and processes
lethal genes
useful to animals
(3) Plant cells are totipotent
(3) Process which use genetically engineered animals
(4) Plants do have cancers
only on a large scale for benefit of mankind
(4) The integration of natural science and organisms
4. Which of the following is not a feature of the
cells, parts thereof and molecular analogues for
plasmid?
products and services
(1) Single stranded
(2) Independent replication
10. The transfer of genetic material from one bacterium to
(3) Circular structure
another through the mediation of a vector like virus is
(4) Small, circular double-stranded
termed as
(1) Transfection
5. During ‘gene cloning’, which is called as gene taxi?
(2) Transduction
(1) Vaccine (2) Plasmid
(3) Bacterium (4) Protozoa (3) Translation
(4) Transformation
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11. Genetic engineering is possible because 18. First recombinant was constructed in the year
(1) DNA can be cut at specific sites by (1) 1963 (2) 1974
endonucleases like DNAase (3) 1918 (4) 1972
(2) Restriction endonucleases purified from bacteria
can be used invitro 19. The cutting of DNA at specific locations became
(3) The phenomenon of transduction in bacteria is possible with the discovery of_____
well understood (1) Restriction endonuclease enzymes
(4) DNA can be seen by electron microscope (2) Probes
(3) Selectable markers
12. Modern biotechnology consists (4) Ligases
(1) Genetic engineering
(2) Tissue culture 20. Term ‘Restriction’ in restriction endonuclease
(3) Microbiology enzyme refers to
(4) All of the above (1) Cleaving of phosphodiester bond in DNA by
the enzyme
13. Plasmid is a (2) Prevention of bacteriophage multiplication in
(1) Fungus bacteria
(2) Protozoans (3) Cutting of DNA at specific position only
(3) Part of plasma membrane (4) Cutting each of the two strands of DNA at
(4) Extra chromosomal DNA in bacterial cell specific points in sugar phosphate backbone

14. Two microbes found to be very useful in genetic 21. Restriction endonucleases are enzymes which
engineering are (1) Remove nucleotides from the ends of the DNA
(1) Escherichia coli and Agrobacterium molecule
tumefaciens (2) Make cuts at specific positions within the DNA
(2) Vibrio cholerae and tailed bacteriophage molecule
(3) Diplococcus sp. and Pseudomonas sp. (3) Recognise a specific nucleotide sequence for
(4) None of the above binding of DNA ligase
(4) Restrict the action of the enzyme DNA
15. Transgenic plants are developed by polymerase
(1) Introduction of foreign gene
(2) Clone and genetically modified genes 22. Identify the plasmid
(3) Purified genes (1) pBR 322 (2) Hind II
(4) None of the above (3) EcoR I (4) AIU I

16. The EFB stands for

23. Consider the following statements
(1) European Forum of Biotechnology
I. Asexual reproduction preserves genetic information
(2) European Foundation of Biotechnology
while sexual reproduction permits variations
(3) European Function of Biotechnology
II. Traditional hybridisation often leads to the
(4) European Federation of Biotechnology
inclusion and multiplication of undesirable genes
along with the desired genes
17. cDNA is
III. rDNA technology allows us to isolate and introduce
(1) Circular DNA
only one or a set of desirable genes without
(2) Cloned DNA
introducing undesirable genes in the target
(3) DNA produced from reverse transcription of
organism
RNA
Which of the above statements are true?
(4) Cytoplasmic DNA
(1) I & II only (2) I & III only
(3) II & III only (4) I, II, & III
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24. The enzyme that seals 5' PO4 and 3' OH polynucleotide 25. Which of the following is a palindromic sequence?
ends while creating a recombinant DNA molecule is (1) 5'-CGTATG-3’
(1) Alkaline phosphatase 3'-CGAATG-5’
(2) DNA ligase (2) 5'-CGAATG-3’
(3) DNAse 3'-GCATAC-5’
(4) Restriction endonuclease (3) 5'-GAATTC-3’
3'-CTTAAG-5’
(4) 5'-GACTAC-3’
3'-CTTAAG-5'
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Note: Kindly find the Video Solution of DPPs Questions in the DPPs Section.

Answer Key
1. (3) 14. (1)
2. (1) 15. (1)
3. (3) 16. (4)
4. (1) 17. (3)
5. (2) 18. (4)
6. (2) 19. (1)
7. (4) 20. (2)
8. (1) 21. (2)
9. (4) 22. (1)
10. (2) 23. (4)
11. (2) 24. (2)
12. (4) 25. (3)
13. (4)

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