Name: Lab 2 – STAINS AND MICROSCOPY

LAB 2 RESULTS AND DISCUSSION QUESTIONS

ACTIVITY 2.1

Sketch 2-3 representative cells of each organism you observed in class or on D2L.

Mixed prokaryote & Yeast, whole mount Yogurt bacteria smear Klebsiella pneumoniae
eukaryote
Total magnification
of scope:
400x 1000x 1000x 1000x

Sketch of field of view

https://www.nih.gov/news-events/nih-research-matters/genome-sequencing-tracks-bacterial-outbreak
Spike Walker, Wellcome Images.
QUESTIONS

1. In the mixed prokaryote and eukaryote slide, which cells are cheek cells and which cells are bacteria? What is
the morphology and arrangement of the bacteria in this slide?

2. Are yeast cells prokaryotes or eukaryotes?

3. Based on the Gram reaction you observed for Yogurt bacteria, describe the cell wall of these cells.

4. What staining procedure was likely used to make the slide of Klebsiella? Why was this stain used?
Name: Lab 2 – STAINS AND MICROSCOPY

ACTIVITY 2.2 and 2.3

Bacillus subtilis Escherichia coli Hafnia alvei S. epidermidis

Gm stain colour:

String test + or -:

Is this cell Gm+ or Gm-?

Total magnification:

Sketch of field of view

Cell shape:

Cell arrangement:

QUESTIONS:

1. Compare the cell wall and membrane structures of B. subtilis and E. coli.

2. Complete the table by naming the step, the reagent used and drawing in a simple diagram of Gm+ and Gm-
cells showing their cell wall/membrane structure and the appearance of the dyes at each step.

Step Reagent Gram + Gram -