1.

8 Factors affection enzyme action

AQA Biology Follow up sheet

To investigate the effect of varying pH on the

rate of a reaction catalysed by trypsin

Specification references
 3.1.4.2

Learning objectives
After completing the worksheet you should be able to:
 carry out enzyme reactions using trypsin and different pH levels
 draw a graph of the results of the reactions
 use the results of the reactions to make conclusions about the rate of
reaction and pH level.

Recap
You have carried out an enzyme reaction using trypsin and different pH levels. An
enzyme reaction is one of the required practicals that you have to do as part of your
Biology A Level. You may be asked questions about this practical in your exams.
This follow-up sheet aims to prepare you for possible exam questions you may be
asked about this practical.

Questions
1 This graph shows the rate of reaction of pepsin at different pH levels.

a Describe the pattern of the graph. (2 marks)

b Explain the pattern of the graph. (2 marks)

© Oxford University Press 2015 www.oxfordsecondary.co.uk/acknowledgements

This resource sheet may have been changed from the original 1
 1.8 Factors affection enzyme action
AQA Biology Follow up sheet

2 Five wells were pressed into an agar plate containing starch. Each well contains
amylase in a different pH. The grey areas show where the agar has been digested.

Explain the results shown on the agar plate. (3 marks)

© Oxford University Press 2015 www.oxfordsecondary.co.uk/acknowledgements

This resource sheet may have been changed from the original 2