Angeles University Foundation

Integrated School
Academic Year 2023-2024

Laboratory Activity:
Extraction of Deoxyribonucleic Acid (DNA)

Complete Name Specific Contribution Signature

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Objectives & Learning Competencies

At the end of the laboratory activity, the learners shall be able to:
✔ Explain how DNA can be extracted from a living sample or source; and
✔ demonstrate or execute proper method of DNA extraction.

Introduction
All organisms contain DNA--the information storage molecule. In fact, all organisms
share at least 500 of the same genes. Changes in DNA lead to biological evolution. In
this activity, you will extract DNA and see what it looks like. Scientists use variations of
this method to isolate DNA from all kinds of organisms.

Materials
Laboratory Gown or Gloves Isopropyl Alcohol
Coat Hair Net or Cap Blender
Printed Laboratory Mung Beans Barbeque Stick
Worksheet Disposable Plastic Cup Meat Tenderizer
Writing Materials: Spoon Strainer or Cheesecloth
Ballpen and Pencil Clear Dishwashing
Cellphone Liquid
Methodology/Procedures and Illustrations

Step 1:
-Soak Mung beans in H2O overnight and then remove hulls.
-Put the Mung beans without hulls into the blender and blend for 15 seconds.

Paste picture here. (5 points)

Step 2:
-Pour the soup through a strainer or cheesecloth into the beaker.
-Add two tablespoons of dishwashing liquid (about 30ml) and swirl to mix.
-Let the mixture sit for five to 10 minutes.
-Carefully pour the mixture into the test tube.

Paste picture here. (5 points)

Step 3:
Add a pinch of meat tenderizer into the test tube and stir gently. Be careful! If you
stir too hard, you'll break up the DNA, making it harder to see.

Paste picture here. (5 points)

Step 4:
Tilt your test tube and slowly pour alcohol into the test tube down the side so that
it forms a layer on top of the Mung bean mixture.

Paste picture here. (5 points)

Illustration of DNA
Paste picture here. (5 points)

Guide Questions
1. What did the DNA look like? How was the DNA similar to and different from the
way you thought it would be? (5 points)

2. Explain the role and importance of the following substances or materials in the
extraction of DNA. (5 points each)
(1) Blender
(2) Dishwashing Soap
(3) Meat Tenderizer
(4) Alcohol

Conclusion
Reference
Bayquen, A., Pavico, J., Ramos, A., Ramos, J., & Silverio, A. (2023). Exploring Life
Through Science Series: Laboratory Manual (Second Edition). Phoenix
Publishing House, Inc.

Javier, Mark Archei O. (2017). General Biology 2. DIWA Learning Systems, Inc.

Genetic Science Learning Center. (2018, October 23). How To Extract DNA From
Anything Living. Retrieved September 23, 2022, from
https://learn.genetics.utah.edu/content/labs/extraction/howto/

Prepared by:

Jezrel Given D. Cortez, LPT

JHS Instructor