NAME- SAGAR DAS

CLASS - XI
SEC - A
ROLL - 26
SUBJECT - BIOLOGY
SESSION -2019-2020
 CER
TIFI
CAT
This is to certify that the investigation work
entitled:

E
“STUDY OF EFFECTS OF ANTIBIOTICS ON MICRO-
ORGANISMS”
Has been conducted by SAGAR DAS, student
of TIGPS, Garia.
He had submitted this project during the
academic year 2019-20 towards partial
fulfilment of requirement of CBSE Board.

Teacher’s signature Head of the Institution

Signature
 ACKNOWLEDGEMENT

I would like to express my special thanks of

gratitude to my teacher, SRIJANI NAG as well as
our Principal, SUJATA GHATAK who gave me the
golden opportunity to do this wonderful project
on the topic “STUDY OF EFFECTS OF ANTIBIOTICS
ON MICRO-ORGANISMS”, which also helped me
in doing a lot of Research and I came to know
about so many new things. I am really thankful to
them.

Secondly, I would also like to thank my parents

and friends who helped me a lot in finalizing this
project within the limited time frame.

STUDENT’S SIGNAUTURE
 INDEX
Page
SL. No. Topic
No.

1 Introduction 5

Aim, materials required and

2 6
Experimental Procedure

3 Diagram 8

3 Observation 9

Precautions, Conclusion and

4 10
Bibliography
 INTRODUCTION
Antibiotics are the organic secretion produced by
micro – organisms, which in low concentrations
are antagonistic to the growth of other micro-
organisms (mostly pathogens). Antibiotics have
proved very useful in combating several bacterial
diseases in man and animal. Antibiotics are
commonly obtained from Actinomycetes and
some eubacteria. Some of the important
antibiotics are Streptomycin, Aureomycin,
Terramycin, Chloromycetin, Erythromycin,
Neomycin etc.

Soil is a natural medium that harbours several

types of micro-organism. These micro-organisms
can be grown on culture media. The effect of
different types of antibiotics can be studied on the
growth of micro-organism growing in culture
medium. This is an important subject, therefore,
the study of effect of antibiotics on micro-
organism has been taken for the present project.
AIM
To study the effect of antibiotics on micro-
organisms.

MATERIALS REQUIRED
Potato, agar, dextrose, distilled water, four
different types of antibiotics (such as penicillin,
streptomycin, aureomycin, terramycin), syringe,
oven sterilized petridish, flasks, beakers, pipettes,
garden soil, glass, marker pen etc.

EXPERIMENTAL PROCEDURE
A. Preparation of culture Medium
Potato Dextrose Agar (PDA) Medium: 200 g of
peeled potato chips taken. They are then
boiled with 500 ml of water in a beaker for 15
minutes. The squeezed potato pulp thus
obtained through a muslin cloth and kept it in
a flask. 20 g of agar is taken in a beaker and
warmed with 500 ml of water. Both the
solution of potato and agar mixed and 20 g
dextrose is added to it. Thus one litre of PDA
medium is prepared.
 Autoclave the medium at 15 pounds pressure
for 15 minutes.

B. Effects of antibiotics on soil micro-

organisms
i) 2 g of soil is taken and dissolved in 10 ml of
water in a beaker. Let the soil particle settle
down.
ii) 5 oven sterilized petridishes is taken and 1 ml
of soil suspension is poured in each of the
plates. Now 1ml of the four antibiotics is
poured separately in four petridishes with the
help of syringe, and marked with marker pen.
The fifth petridish is left without antibiotic to
serve as control.

iii) PDA is poured in each of the petridishes and

the suspension is mixed by rotating the
petridishes. The petridishes are left
undisturbed at a warm place for a week.
Culture Medium in Petridishes for Culturing
Soil Micro-organisms.
OBSERVATION
Colonies of micro-organisms appeared on the
culture medium, the colonies in each petridishes
is counted and presented my observation in the
following table:

S.No No. of colonies in a

Antibiotics
. petridish
1. Penicillin NIL

2. Streptomycin 2-3

3. Terramycin NIL

4. Chloromycetin 1-2

5. Control 30-40

The effect of different antibiotics on the micro-

organisms were assessed by counting the number
and size of the colonies growing in the
petridishes.
PRECAUTION
1.Did not expose the culture of petridish to the
atmosphere.
2.Sterilise the petridishes properly in the oven
before use.
3.Use proper kind of stains for different type
micro-organisms.

CONCLUSION
Pencillin and Terramycin were the most effective
antibiotic against micro-organism in soil.

BIBLIOGRAPHY
 Comprehensive Laboratory Manuals in Biology.
https://www.1000sciencefairprojects.com/Biology/a
ids-and-hiv.php
 https://www.google.co.in/

 https://www.wikipedia.org