Natural Sanitizing Ingredient

Product Name: MLE-T

Natural Sanitizing Ingredient (MLE-T)
1. Fast and broad anti-bacterial and anti-fungal properties
2. Provides optimal cleanness yet safe for skin
3. Environmental-friendly

4. Possible applications
1. Baby (feeding) bottle cleaner (Prevents the infection from bacteria)
2. Hand sanitizer (Alcohol free-possible moisturizing effect)
3. Hand cleaner (Moisturizing and no irritation)
4. Wet tissue (No irritation and prevents the infection from bacteria)
5. Food grade sanitizer/cleaner (Natural ingredients and Food grade)
 Ingredients

Licorice
Common name Licorice
GLYCYRRHIZA GLABRA ROOT
INCI NAME
EXTRACT
Scientific Name GLYCYRRHIZA GLABRA
The compound glycyrrhizic acid is routinely used for the treatment
and control of chronic viral hepatitis. Recent studies indicate that
glycyrrhizic acid disrupts latent Kapsi’s saarcoma (as also demonstr
ated with other herpesvirus infections in the active stage), exhibiting
a strong anti-viral effect. The primary antioxidant and anti-inflamma
tory compounds found in licorice root are the glycosides - glycyrrhi
zin and glycyrrizinic acid, flavonoids, and saponins

Anti-viral, anti-aging, anti-oxidant,

anti-inflammation
 Ingredients

Green tea
Common name Green tea
INCI NAME Camellia Sinensis Leaf Extract
Scientific Name Camellia sinensis L.

Green tea inhibits the growth of bacteria, and contains the

ingredients which prevent and treat acnes. Green tea polyphenols
have the effects of astringency, pores contraction, and anti-aging.
It contains a lot of vitamin C which reduce the liver spots, freckles
and whiten skin. Vitamin A, B2, C, E in green tea remove the harmful
acid and enhance the elasticity of the skin. Catechin of green tea
helps control the production of sebum to make clear T-zone and
cheek.

Antimicrobial, anti-aging, elasticity

 Ingredients

Lactobacillus
Common name Lactobacillus
INCI NAME Lactobacillus Ferment
Scientific Name Lactobacillus

Lactobacillus is a genus of Gram-positive facultative anaerobic or

micro-aerophilic rod-shaped bacteria. They are a major part of the
lactic acid bacteria group, named as such because most of its
members convert lactose and other sugars to lactic acid
They possess potential therapeutic properties including anti-
inflammatory and anti-cancer activities, as well as other features of
interest.

Antimicrobial, anti inflammation,

prevent skin trouble
 Fermentation
Increases organic acid Increases anti-
Fermentation
and decrease pH microbial activity
 Bioconversion

Before Bioconversion After Bioconversion

Bioassay Bioassay

Silica gel column (4.6mmx25cm) , Water:MeOH:0.1NHCl = 94:5:1

17
 MLE-T

Indicated substance
Glycyrrhetinic acid
MLE-T
Challenge test – Wet Tissue

- Method
1. Each 0.3% and 0.5% solution of MLE-T , Benzoate , phenoxyethanol
was used in this experiment.
2. Add 5g of each solution to the wet tissues.
3. Inoculate 100ul of S.aureus, and E.coli (cell concentration 106cfu/ml) to
the wet tissues.
4. Place each sample onto the PCA medium.
5. Incubate at 35℃ for days.
5. Count the cell number of each sample.
 Wet Tissue

1 2

1. Spray the sample onto the tissue. 2. Inoculate the cells.

3 4

3. Incubate the tissue at RT for 7 days. 4. Check the microbes after diluting the
samples with autoclaved water
- Result

S.aureus E.coli
Control 106 cfu/ml 106 cfu/ml
MLE-T 0.3% >99.999% >99.999%
MLE-T 0.5% >99.999% >99.999%
Benzoate 0.3% 99.98% 98.8%
Benzoate 0.5% 99.98% 99.77%
phenoxyethanol 0.3% 99.98% 99.98%
phenoxyethanol 0.5% 99.87% >99.999%
Challenge test - detergent
- Method
1. Prepare 1% stock solution of MLE-T.
Dilute 1:10 in natural surfactant to make 0.1% of MLE-T.
Prepare MLE-TC.
2. Inoculate S.aureus, and E.coli to 0.1% MLE-T and MLE-TC.
* cell number (106cfu/ml)
3. Incubate at room temperature for 8 hours and count the cell number.
•MLE-TC : BST’s natural preservative for detergent. You can make
MLE-TC by mixing it with natural surfactant.
(MLE-T : natural surfactant =0.02:19.98)
- Result S.aureus E.coli
Control 106 cfu/ml 106 cfu/ml
MLE-T 99.999% 99.999%
MLE-TC >99.999% >99.999%
 Anti-bacterial efficacy MLE-T

Control 1min 5min 24hour

-Sample : 10% and 5%

S.aureus solution of MLE-T
-Test Time : 5min

- 5% or more of MLE-T can

<E. coli>
inhibit the microbes in 5
E. coli minutes.

<S. typhiurium>

S. typhiurium

Sample Antibacterial efficiency

S. aureus E. coli S. typhiurium
5% MLE-T 99.999% 99.999% 99.999%

10% MLE-T 99.999% 99.999% 99.999%

 Disinfection of fungi

<GSE 1%> <MLE-T 1%>

(After 7days)

<70% EtOH%> <Not-treated>

 Gauze was soaked in each sample and let it dry. The dried gauze was put on the PDA and 200 μl
of A.niger (104cfu/m) was inoculated onto the gauze.
The plate was incubated at 30 o C for 7 days and checked if the fungi appeared.
 물티슈 challenge test MLE-T
1. 부직포 3*3cm2 면적으로 잘라 멸균
2. 0.5, 0.8, 1.0% MLE-T (10㎖) → 부직포에 점적
3. Bacteria+Yeast & Mold 각각 106cfu/ml 접종 → 30℃/7일간 보관
MLE-T (%)
Medium
0.5 0.8 1.0

PCA

PDA
Thank You

B208, Wollimlions’ s Valley II B-Line,

14 Sagimakgol-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do,
462-120 Korea
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