BD CytoRich™

non-gyn

BD CytoRich™ non-gyn – one solution for all your non-gynecologic

cytology needs. The easy to use process consistently provides
better slide quality, and for you, a higher degree of confidence.
BD CytoRich™ non-gyn process combines the fixative properties
of BD CytoRich™ preservatives and the discrete batch processing
capabilities of BD PrepStain™ Slide Processor, to provide you with
one solution for all your non-gynecologic cytology needs.

THE BD CYTORICH™ NON-GYN PROCESS:

a Consistently provides clear slides that are free of background debris1,2,3,4. This allows for better
evaluation of cytologic features and interpretation of specimens1

a Eliminates the necessity to create multiple slides for each specimen. One slide per specimen means
quicker processing and screening times2

a Compatible with specimens from any non-gynecologic body site

a Allows to perform adjunctive tests like cell blocks, molecular tests, immunocytology, flow cytometry, etc.2

Bronchial Washing Fine Needle Aspirate (Pancreas) Body Fluid (Peritoneal)

Body Fluid (Peritoneal) Body Fluid (Pleural) Urine

 General Non-Gyn Procedure
Including Urines, Body Cavity Fluids, FNAs and Respiratory Specimens

STEP 1: CONCENTRATE
4 Mix to homogenize sample
4 Assess for and remove clots,* particles* and mucus**
4 Transfer to 50 ml centrifuge tube
4 Centrifuge for 10 minutes @ 600 g
4 Pour off supernatant fluid
* Can be used for cell block procedures
** Follow standard laboratory procedures to homogenize and/or emulsify mucus

STEP 2: FIX
(This step may be omitted for prefixed samples, vortex samples for 15 +
_ 5 seconds
and proceed to the next step)

4 Add 5 – 10 ml of BD CytoRich™ Red or Blue Preservatives

4 Vortex and let sit for a minimum of 30 minutes
4 Centrifuge for 10 minutes @ 600 g
4 Decant supernatant and vortex

STEP 3: WASH
4 If no visible or small pellet is identified, add 6 mL of buffered DI H2O,
vortex and transfer entire contents to 12 mL tube
4 If moderate to large pellet is identified, transfer a representative
sample (1-5 drops) to 12 ml tube and add 10 ml water
4 Centrifuge for 5 minutes @ 600 g
4 Decant and vortex to homogenize sample

STEP 4: PROCESS
4 Load the labeled 12 ml centrifuge tubes onto BD PrepStain™ Slide Processor
for processing
4 Follow ‘Non-Gyn Slide Processing’ instructions in BD PrepStain™
Slide Processor Operators manual

For detailed procedure, please contact your local BD Diagnostics – TriPath Representative or refer to BD CytoRich™ non-gyn procedure manual.
BD CytoRich™ preservatives help in providing
overall clear slides that are easy to interpret

BD CytoRich™ Red BD CytoRich™ Blue

Lyses RBC’s and Preserves RBC’s
solubilizes protein and proteins

The BD PrepStain™ Slide Processor improves workflow in your lab

by providing discrete batch processing and staining capabilities.
a Consistently prepares thin-layer slides that display better
slide quality than conventional slides1,2,3,4

a The discrete processing and staining capabilities eliminates

the necessity to triage high-risk specimens and reduces
cross contamination issues

a Improve BD PrepStain™ Slide Processor utilization by

processing both gynecologic and non-gynecologic specimens

Performance of the various cytopreparatory techniques for malignant or suspicious fluids

Smear, MGG stain

2000 Data: Cytocentrifuge samples,

MGG stain
71 samples
BD PrepStain™ [BD CytoRich™
(Prospective non-gyn] Papanicolau stain
trial 3)
Block

Smear, MGG stain

1998 Data: Cytocentrifuge samples,

MGG stain
54 samples
(Retrospective Smear, Papanicolaou stain

literature Cytocentrifuge samples,

review 3 ) Papanicolaou stain

0- 1 5 10 15 20 25 30 35 40

False Negative Rate (%)

An independent study found that BD PrepStain™ Slide Profiler (BD CytoRich™ non-gyn) thin layers
showed a significantly lower false negative rate (p=0.0414) versus conventional cytopreparatory
methods (Gabriel et al. Acta Cytologica 2004; 48:825-835)
 Fine Needle Aspirate Fine Needle Aspirate Urine
(Lymph Node) (Thyroid)

ORDERING INFORMATION
Please contact your local BD Diagnostics – TriPath representative.

1 As compared to conventional methods. Weidmann et al. Acta Cytol 1997: Jan-Feb ;41(1):182-7
2 Veneti S et al. Acta Cytol. 2003 Mar-Apr; 47(2):188-92
3 Nicol et al. Acta Cytol. 2000; 44(4):56-75
4 Gabriel et al. Acta Cytol. 2004;48:825-835
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