Date 6/as/20L2

yoti-1e000.566
Expt.No. 19 Page No.
Expenimerd-9
Aim
lo evaluat the local aneshabic by_alilleent melhada.
Keerence.
Meslhi, 8. ; Rakash, A..Raclical Manual eExperimenlal and Clintcal
harmacologyJaypee Brothers Medlical Pubksthexs(2) Ld, f ediHion,
IDI0, ta no.- R28-LR9

hequiement.s
Animal's freg, RabbiB Cquinea pia
Solution'g Rocainehydmehloride stock seluhion_(17,ly), HCL(a1n)
Eguipmentie Frog boar , Rabbit holder, Catbon wiek Ssusica
instruments

Learming_ehjechives z
Dirpart ho knaucledae lacal ansthlie elet o
dngs.
heozy
Local anesifalos eversibly block _impulse conolucthon_
aloms_nenrve
axond and excitable _menbranes.
Their
he tr achion i useol do pmduce docal or eionak aneslhesia
and or _blocking pain senaaion.
The local ansahehie pnpei can he easilshudiedby sing
any thu Jollausing mehad
Neave Block Anesthesla Lsollman mehoe) ushere Ha dug ahhlied clese
o nent unk.
6)SunfaceAncasthesin adroinister tka drg inb he conjuncltva el he
2ye and Shuoy coneal nlax n painkd object
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16Jp3 2D3
Epenintnt-9 yoi-120o0KB&
Paze na-Y4

1oevaluati he local andeslhehe by dillornd methods.

Pbsewaion dable
Medhod Parameler Response belore | Response aler
No. reatme treament
4. Nerve block (plexus) foot-10hohasl Jmmediab Alayed
aneshes ia potedune relax
|2. aSurfote anawthesto Caneal relex Present Absens
pv cedurt

3. Jnillerakon anashusia Squeaking o Presend bsent

prn ceolurt toltehin responde
 o d i - lO00 S 66 Data /os/20

Expt. No. 9
PageNo.

nfHesaion_anesthesia shere hu dng is_adminisdered intsadecmally

and injechion diti Tested for reaction t pinpichs..
racedure
1 Nerve Block pexuus anesthesia l{st-woithdraal rler in eg
aDecenbral1d the bng iao-150g) ond deshoyed ha upper cpor o he
spinal cord codhhe help o pithing needle
needle
L)Cut opened the abdomen and emoved all the abdorainal organs,so
hat a puch uwa4_ made e ahdominal usalls.
Exbssud he spinal nerves n the cavity
dPit the ngon fhe fg boorod oth hoo_ol Pk hind legs hanstng
eepm boand. lernahivel, he may be pinñtde t
he boorcd placeo verhicall sothat i ind legs hans e.
eImmensed haMiglt hind n dhe beaken containad o N HCce
Nofed he briekxellex eohoraaolel ha
le
hwhshedu imnmesded leq elitk lozoing salan or nomal saline
Kepealud Same aifh th l t hind lg.
Wnshed dks immexsed les
Placed ahout 10 ml e 7 y pmcaine hydmchleride n he
ahdominal pauch. he siadionere as exposed to the Local
_anesthehie ackons e he dhug. Alased h drug da act or 5minaia
Smnnersedaight_and thind lesa inuceLdaion in hbeaken
Contained_acid as belore.
Notadthe delayedin he llar_Lothdrausl kgu aler 5,10,15
and dominnis e dhug adminiuterafon.

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 yobr-1RoooS66 Date 14/n.3/2022
Expt. No. 9 Page No. 6

Surtace anesthesa
Placed he mbbit in he rabbit holder kept the head outside.
bkxamined -he Coneal ellex_ns toinked response do touchad a side
e he Coanea coih_Coton iece
Shsilled a eo dnp o pmcaint HCL solution in the conjuntiva
in the sh pye ethe rabbit test).
d imultanouslyinsdalleo saline in -he Etye uhichdevEs as conhol
e)Recorded the coineal relhx efler hen _minit dug instillation_and
tabulatid he cbservahion
I.Snfilerahion Anesthesia
a)Shoved hu back o Hu Juinea pia
bInjectud he pancaine IcÍ selchon intrademall
c) h e aninal_shocoed 4he squeaked n_hoikehad wspanse on douchins
h injechon site e0tlh shasppin,2t indicalid he posihia
Aponselpin pick coifhdhausla).

SnferenceRsull-
A. ALct application e pmcaine ydnchloride o he nene runk /ples)
pmduces Local anaesdhe.sia as aot eothdaus! rellexes ane bloc keo
in ha
rocaina daoclloricde pmoluces the Less nl_comeal response in
he inshile eye indicaing local anasthetie elect
CAfkrinjection Pacainehyduochloride abelihes ha squeaking
koitekigg local) Ae pon on touching he injechionsikoidh
shorp pin indicatad hu docal dnaethchie elleek.

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Jyot-2000SE6 Date /o3 2032
Expt. No. Page No. 7

Precaulions 2
Be Carelul ohile perfpoming the experiment
l a n f . nete he cadlirg llex ackon

Leanins Drilcomes
Leant ahot Au loeal anautheiod, depplari zalion
polarization
Learnt he dileent melools do study he local
naedHhehie popeily
Y Karnf o perfpm tha experimeni eith Cae.

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