Experiment No 1.

Isolate DNA from available plant material such as spinach, green pea
seeds, papaya, etc.

Aim

To Isolate DNA from available plant material such as spinach, green pea seeds,
papaya, onion, etc.

Requirement

Plant material, mortar and pestle, beaker, test tubes, filter paper, liquid detergent,
NaCl, ethanol, petri dish, spool etc.

Principle

DNA(Deoxyribonucleic acid) and RNA (Ribonucleic acid) are two type of nucleic acids
found in living system. DNA act as the genetic material in most of the organisms.
RNA, though it also acts as genetic material in some viruses, mostly functions as a
messenger adapter, structural and some cases as a catalytic molecule.

Observation

The addition of ethanol to the solution causes DNA to precipitation. The DNA fibres
appears as white precipitate of very fine threads on the glass spool.

Result

DNA fibre appears as white precipitate of very fine threads on the glass spool.

1
Experiment No 2. Prepare a temporary mount to observe pollen germination

2
Experiment No 3 Prepare a temporary mount of onion root tip to study mitosis.

3
Experiment No : 4 Study the plant population density by quadrat method.

4
Experiment No : 5 Study the plant population frequency by quadrat method.