FIRST YEAR HISTO SLIDES

ALONG WITH IDENTIFICATION POINTS

The following document contains

histological slides pictures which were
observed in practical classes of
academic year 2022
 CVS MODULE:

Artery:
- Media containing thick layer of smooth muscles
as compared to lumen.(along with few reticular
and collagen fibers)
- Thin adventitia with few fibers and loose
connective tissue
- Intima having single layered epithelium
- Epithelium ; simple squamous epithelium
- Lumen is nearly circular
- Presence of RBCs
 CVS MODULE:

Veins:
- Media containing thin layer of smooth muscles
as compared to lumen i.e; larger lumen. (along
with few reticular and more collagen fibers).
- The three layers are no as clearly
distinguishable
- Intima having single layered epithelium
- Epithelium ; simple squamous epithelium
- Lumen may not be circular
- Presence of RBCs and valves may be present in
lumen
 CVS MODULE:

CARDIAC MUSCLE:
- Linearly branched and interconnected cells
- Uninucleated or maybe bi-nucleated.
- Intercalated discs at junctions
- Striations (A and I bands)
 RESPO MODULE:

Trachea And Respiratory Epithelia:

- Epithelium layer of pseudostratified columnar
cells and goblet cells in mucosa
- Thin layer of connective tissue along with fibers.
- Submucosal or seromucous glands are present.
- Cartilage plate along with layer of smooth
muscles.
- Outer loose connective tissue layer.
 RESPO MODULE:

Lung Tissue:
- Presence of bronchioles and alveoli.
- Bronchioles may contain respiratory,
simple columnar or simple cuboidal type
of epithelium (all ciliated) based on the
division of bronchiole.
- Simple squamous epithelium in alveoli.
- No glands and no presence of cartilage.
 LOCO MODULE:

Compact Bones :
Characteristics feature: Osteon
- Haversian canals (concentric circles of
lamellae)
- Collagen fiber arrangements making
lamellae.
- Osteocytes (dark coloured cells)
 LOCO MODULE:

Spongy bone and Compact bone:

- The following sample consist of both
spongy and compact regions of bone.
- Differentiated from their appearance of
harvesian system/osteons.
- Spongy bine will have distorted lamellae
with more space in between and large
harvesian cannals.
- Compact region will comprise of small
harvesian cannal. Well developed
lamellae.
- Lacunae can be seen in both regions.
 LOCO MODULE:

Hyaline Cartilage:
- Bluish, glassy appearance.
- Fibers are present (little to no appearance
because of being in dissolved state).
- Chondrocyte in the form of isogenous
groups.
- Lacunae around cells.
- Pericellular matrix around lacunae and
territorial matrix around pericellular. The
whole light blue stained area is inter
territorial matrix.
 LOCO MODULE:

Elastic Cartilage :
- Unclear ECM with brownish appearance
(possibly due to fiber stains)
- Reticular and elastic fibers clearly visible.
- Presence of chondrocytes.
- Presence of Perichondrium
 LOCO MODULE:

Vertebral disc`s fibrocartilage:

Fibrocartilage :
- Bundles of collagen fibers.
- Chondrocytes lying in rows between
fiber bundles.
 BLOOD MODULE:

Palatine Tonsils:
- Crypts (folding of surface epithelium)
- Lymphoid follicles visible plus most of
them contains germinal centers.
- Capsule of dense connective tissue
 BLOOD MODULE:

Lymph Nodes:
- Lymphoid follicles (primary and secondary)
- Follicles surrounding inner darker area
(medullary cords)
- Germinal center in secondary follicle.

- Best represented in figure 14.6 of Laiq

histology.
 BLOOD MODULE:

Thymus:
- Septa dividing parenchyma into
incomplete lobules.
- Outer Darker cortex.
- Inner lighter medulla.
- Hassall's corpuscles in middle of medulla
 BLOOD MODULE:

Spleen:
- Large amount of RBCs
- Presence of red and white pulp
- Presence of germinal center of lymphoid
follicle in white pulp
- Splenic sinusoids and splenic cords
 FOUNDATION MODULE:

Smooth Muscles:
- Spindle shaped with tapered ends
- Nucleus in between
- Non striated
- Non branching
 FOUNDATION MODULE:

Skeletal Muscles:
- Cylindrical shaped.
- Multinucleated.
- Striations present.
- Non branching Presence of connective
tissue (maybe epimysium or perimysium)
 FOUNDATION MODULE:

Skin, finger tip:

- Epithelium: stratified squamous
epithelium keratinized.
- Multiple layers of cells transitioning from
cuboidal to squamous (flattened cells)
Inside to outside.
- Layer of keratin protein.
 FOUNDATION MODULE:

Large Intestine:
- Epithelium: Simple columnar cells
- Single layer or tall cells with oval nucleus
at basal part.
- Microvilli also present
- Glands : straight simple tubular
 FOUNDATION MODULE:

Pancreas:
- Epithelium: Stratified cuboidal epithelium
- Multiple layered square shaped cells with
nucleus in the middle.
- Glands: Compound acinar at exocrine
portion of pancreas.
 FOUNDATION MODULE:

Kidney:
- Epithelium: simple cuboidal in distal
convoluted tubules
- Single layered, roughly square shaped cells
with nucleus in center.
 FOUNDATION MODULE:

Ureter:
- Epithelium: transitional epithelium
(urothelium)
- Multiple layers of cells.
- Cuboidal cells innermost, polygonal cells in
between, dome-shaped (convex apice)
cells outermost - umbrella cells
- Specialized type of epithelia to withstand
stretch.
 FOUNDATION MODULE:

Gall Bladder:
- Epithelium: Simple columnar cells
- Single layer or tall cells with oval nucleus
at basal part.
 FOUNDATION MODULE:

Urinary Bladder:
- Epithelium: transitional epithelium
(urothelium)
- Multiple layers of cells.
- Cuboidal cells innermost, polygonal cells in
between, dome-shaped (convex apice)
cells outermost - umbrella cells
- Specialized type of epithelia to withstand
stretch.
 FOUNDATION MODULE:

Thyroid:
- Epithelium: simple cuboidal
- Single layered, roughly square shaped cells
with nucleus in center.
 FOUNDATION MODULE:

White Fibrous Connective Tissue Of

Tendon:
- Band like pattern of arrangement of
collagen fibers running parallel to each
other.
- White in appearance.
- Small quantity of ground substance.
 FOUNDATION MODULE:

Loose Connective Tissue:

- Connective tissue layer containing all three
basic components of connective tissue.
- Ground substance containing fibers
- Connective tissue cells (fibroblast, mast
cell, adipocytes etc)
- Presence of blood vessels in ground
substance.
 FOUNDATION MODULE:

Adipocytes:
- Fat droplets (yellowish to brownish
appearance)
- Maybe unilocular or multilocular
- Nucleus in periphery.
- Signet ring appearance in unilocular
adipocytes.
 FOUNDATION MODULE:

Nerve Cell/Tissue:
- Nerve cells in longitudinal section
- Perineural cells (in contrast to epithelium)
running parallel with the neural cells
fascicle.
- Endoneurium (delicate connective tissue
around myelin sheath) surrounding each
neuron.)
 FOUNDATION MODULE:

Blood Smear:
- Giving information about number and
shape of different blood cells.
 FOUNDATION MODULE:

Isolated squamous epithelium :

- Single layered flattened cells.
- Polygonal mosaic appearance from surface
view but spindle shaped in side view.
- Nucleus in the middle.