Professional Documents
Culture Documents
PMLS1
PMLS1
1866 ▹GREGOR MENDEL - law of inherited ● From these cultures, it can again
characteristics from studies on plans be obtained
1928 ▹ ALEXANDER FLEMING - initiating 1920 ▹ First clinical laboratory for serum
antibiotic era phosphorus
▹ use of venipucnture for diagnostic testing
OTTO FOLIN - developed quantitative (widespread)
analytical methods for several urine
analytes 1921 ▹ First clinical laboratory method for
● Urea, Ammonia, Creatinine, Uric sertum magnesium
Acid, Total nitrogen, Phosphorus, 1922▹ASCP (American Society of Clinical
Chloride, Total sulfates, Acidity Pathology) was foujnded in St. Louis,
1902 ▹ KARL LANDSTEINER - discovered Missouri
human blood types and described ABO
blood group 1926▹ ARNE TISELIUS - developed moving
↪ He studied bleeding in newborn babies boundary proteins
▹ THEODORE SVEDBERG - determined the ▹ ALEXANDER GUTMAN - first assay for
molecular weight of haemoglohbin by acid phophatase
ultracentrifugation
1939 ▹ EDWARD JOSEPH CONWAY &
1928 ▹ GEORGE NICHOLAS ROBERT COOKE - first clinical laboratory
PAPANICOLAOU - first recovered the ability method for blood ammonia
to recover cancer in vaginal smears ▹AMERICAN MEDICAL TECHNOLOGISTS
↪ CLINICAL CYTOLOGY (AMT) - founded
1930 ▹ H.D KAY - the first clinical 1945 ▹ S. BORGSTROM - whole blood
laboratory method for alkaline phosphorus clotting time test
↪ CLINICAL ENZYMOLOGY
▸REFRACTOMETRY - first used in clinical 1946 ▹BECTON DICKINSON CO. -
laboratories for the determination of vacutainer evacuated serum collection tube
protein in urine
▸ASCP - first medical technologist 1947 ▹ AMERICAN ASSOCATION OF
medication to PAUL ADAMS from Fort BLOOD BANKS - founded
Wayne, Indiana
▸BECKMAN INSTRUMENTS 1948 ▹ AMERICAN ASSOCIATION OF
CLINICAL CHEMISTRY - founded
1932 ▹ IAN CHERRY AND LATHAN
CRANDALL - clinical laboratory method for 1950 ▹ROSALYN SUSSMAN YALOW &
serum lipase activity SOLOMON BERSON - radioimmunoassay
▹ S. LEVEY & E.R JENNINGS - adapted the
1935 ▹ BECKMAN INSTRUMENTS CO. - Shewhart QC chart to use in clinical
first pH meter laboratories
▹ ASCP BOARD OF REGISTRY - first
required a single college degree for medical 1952 ▹MIROSLAV POULIK -
technologist medication immunoelectrophoresis
1937 ▹COOK COUNTY HOSPITAL - first 1954 ▹ JONAS SALK - developed
blood bank poliomyelitis vaccine
▹ S.A KUBY - developed the clinical
1938 ▹MICHAEL SOMOGYI - two major laboratory method for serum creatinine
medical laboratory methods for serum and phosphokinase activity
urine amylase activity
▹ ALAN WALSH - develops the atomic 1965 ▹ Scanning electron microscope
absorption spectrometer
1967 ▹GARRY ABELEV - alphafetoprotein
1955▹FELIZ WROBLEWSKI & JOHN (AFP) is elevated in serum of patients with
LADUE - clinical laboratory method for testicular teratocarcinoma
serum lactace dehydrogenase ▹ US - enacted the Clinical laboratory
▹ ARTHUR KARMEN - clinical laboratory improvement act (CLIA ‘67)
method for aspartate aminotransferase
▹ SEVERO OCHOA - synthesized RNA 1968 ▹ DUPONT (THE ACA) - First
random-access analyser
1956 ▹FELIZ WROBLEWSKI & JOHN
LADUE - method for serum alanine 1969 ▹ ANALYTICAL CHEMISTRY - high
aminotransferase activity called “serum performance liquid chromatography (HPLC)
glutamic pyruvic transaminase”
↪ recognized specificity for liver disease 1973 ▹ JAMES WESTGARD - Westgard
compared with that of aspartate control rules into clinical laboratory quality
aminotransferase control
▹JOHN EDWARDS - proposes prenatal
screening for genetic disease 1975 ▹ Laser cell sorter
▹ROCHE DIAGNOSTICS - first
1957 ▹ EMILE VAN HANDEL & DON commercialized carcinoembryonic antigen
ZILVERSMIT - develops a direct chemical (CEA) assay
method for the determination of
triglycerides 1976 ▹ MICROMEDIC CORP. - first
automated radioimmunoassay
1959 ▹ TECHNICON CORP. - first clinical
laboratory clinical analyser, the 1979 ▹ M.C. YANK - prostate specific
single-channel “Auto-analyzer” antigen (PSA) as a serum tumor marker
⇀ first applied flame photmetry to
automated methods 1980 ▹ BARUCH SAMUEL BLUMBERG -
Hepatitis B vaccine
1960 ▹methods for serum creatinine ▹ DAVID COLCHER - CA-72 serum tumor
phosphokinase isoenzymes marker, primarily for colorectal cancer
▹first method for gamma-glutamyl
transferase (GGT) in serum 1981 ▹ HILARY KOPROWSKI - CA-19-9 as
▹PERKIN-ELMER CORP - introduced a serum tumor marker for pancreatic
atomuic absorption spectrometry (AAS) for marker
the determination of calcium and ▹ ROBERT BAST JR. - CA-125 as a serum
magnesium tumor for ovarian cancer
▹ FEICHMEIER - mechanical pipettor
(autodilator) 1983 ▹L. LINDHOLM - CA-50 as a serum
tumor marker for colorectal cancer
1962 ▹ A.M. SIEGELMAN - method for 1987 ▹KARY MULLIS - polymerase chain
glutamic dehydrogenase reaction (PCR)
▹ INTERNATIONAL SOCIETY FOR ▹ R. TOBIAS - CA-15-3 as a serum tumor
CLINICAL LABORATORY TECHNOLOGY marker for breast cancer
1987 ▹ KURT BRAY - CA-549 as a serum the FIRST RESERVE HOSPITAL by Lt. Col
tumor marker for breast cancer Henry Lipincott
▹S. FUKUTA - CA-195 as a serum tumor ↪ Chief surgeon of the Division of the Pacific
marker for colorectal cancer and 8th Army corps.
1966 ▹ R.A 4688 (CLINICAL LABORATORY 1975 ▹ UST graduate school offered Master
ACT) approved of Science in Medical technology (MSMT),
first school to offer
1969 ▹PAMET was registered at the ▹ PIONEER EDUCATIONAL REVIEW
Securities and Exchange Commission CENTER (PERC) - first review center
▹ R.A 5527 (Philippine Medical Technology
Act_ enacted into law 1978 ▹ Medical Services of America, INC
(MSA) tapped BSMT graduate to undergo a
1970 ▹ Board of Medical technology created 6-month on the job Respiratory Theraphy
pursuant to R.A 5537 Training program
▹ First licensure examination for Medical ▹ 1st batch of Filipino Respiratory
technology therapists
▹ MARIDEL P. PASAGA - first board ▹ R.A. 5527 further amended by P.D 1534
topnothcer
1981 ▹ Research Institute for Tropical ▹PAMET President NORMA CHANG was
Medicine was formally established with the elected President of AAMLT
signing of E.O 674, research facility under
DOH 2000 ▹ DOH reoganized
▹ Bureau of Reseach and Laboratories
1983 ▹ Philippine Blood Coordinating abolished and licensing of laboratories was
Council was created transferred to the Bureau of Health
▹ PROFESSOR LINA C. SOMERA - UP College Facilities and Services
of Public Health ▹PCQACL was registered
↪“MOST OUSTANDING MEDICAL
TECHNOLOGISTS” 2002 ▹ Philippine Society of Medical
Technology Students (PHISMETHS)
1985▹PAMET gained membership in the organized
ASEAN Association of Medical Laboratory
Technologists (AAMLT) 2004 ▹ PWU stated offering Certicicate in
Phlebotomy, the 1ST TESDA certified
1986 ▹PAMET hosted the 2ND ASEAN short-term course of phlebotomy
Conference in Medical Laboratory
Technologists in Manila where AMMLT 2005 ▹American Society of Clinical
constitution and by-laws were adopted Pathology Board of Registry introduced
ASCP International Cerification in the
1988 ▹ Philippine Women’s University Philippines
submitted for PAASCU Accreditation, ▹ ACTS Review Center
↪ the first school MT school to undergo ↪ “THE NATION’S MOST OUTSTANDING
PAASACU accreditation MEDICAL REVIEW CENTER”
▹Olangapo-Zambales Chapter, awarded by
PAMET 2006 ▹ Schools and Universities updated
↪ “MOST OUSTANDING CHAPTER” their curriculum and changed the name of
BSMT to BMLS (Bachelor of Medical
1989 ▹3RD ACMLT in Singapore, PAMET laboratory Science) of CHED
President CARMENITA ACEDERA elected
to the AAMLT presidency 2009 ▹ The first annual Medical
Technology Student Congree was held at
1996 ▹PUC changed name to Adventist Our Lady of Fatima University – Valenzuela
University of the Philippines Campus;
▹ the National Kidney and Transplant
1997 ▹PAMET hosted 7TH ACMLT at PICC Institute (NKTI) Medical Laboratory gets
the first ISO 15189: 2007 accreditation by
1998 ▹ ACTS Review Center established the Philippine Accreditation Office (PAO) in
CEU integrated Emergency Medical the Philippines.
Technician (EMT) course to their BSMT
curriculum 2010 ▹ The first batch of BMLS students
graduated
1999 ▹ The Philippine Council for Quality ▹ The first annual Medical Technology
Assurance in Clinical Laboratories Student Leadership Training and
(PCQACL) was organized Strategic Planning was held at ATI-CAR
Benguet State University in La Trinidad, ▸auxiliary branch of medicine which deals
Benguet. with the examination of tissues and body
fluids using various chemical, microscopic,
and other medical laboratory procedures or
techniques that will aid the physician in the
diagnosis and treatment of diseases and in
the promotion of health in general. (RA
5527)
Detection
Diagnosis
Treatment of Disease
● PROCEDURE OF SCIENTIFIC
ACTIVITIES
● A PARAMOUNT FIELD OF
SCIENTIFIC INVESTIGATION
● AN INTERVENTION IN MEDICAL
PROCEDURES
● EXPLICIT APPLICATION OF
SCIENCE AND TECHNOLOGY
● CIRCUMSTANTIAL MEDICAL
EVIDENCE
BONE TISSUE
According to JEANNE M. CLERC (1992)
TESTING MAY BE: ▸is a profession concerned with providing
QUANTITATIVE, SEMI-QUANTITATIVE information based on the performance of
analytical tests on human body substances of
QUALITATIVE, OR detect evidence of or prevent disease or
DESCRIPTIVE
impairment and to promote and monitor
PHYSICAL AND
CHEMICAL good health.
ANALYSES
▸is a branch of medicine concerned with the ● Biological and Medical Research
performance of laboratory determinations
● Prevention and Control of Diseases
and analyses used in the diagnosis and and Infection
treatment of the disease and the maintenance
of health. ● Innovative Techniques and
Technologies
● Be Honest in Practice
● Demonstrate Professionalism
● Uphold Confidentiality
▹ HISTOTECHNOLOGIST
▸is a Medical Technologist who has
undergone specialty training in
Histopathologic and Cytopathologic
techniques.
▸a Medical Laboratory Technician who has
undergone the same specialty training is
called a Histotechnician.
▸ processes tissues removed during surgery.
Diagnostic stool
▹ BENCH
Serves as a motivation for improvement
▸the central workstation and testing area in
Provides information on student’s a laboratory
response to a leraning strategy ▸Hands-on technical work that is performed
on a daily basis
Determines the demonstrable changes in
▸Monitoring of quality control programs
the attitude and behavior of students
▸ Trobuleshooter who knows how each
piece of equipment works and how to fix it
TYPES OF ASSESSMENT ▸ work in the research and development
▸ FORMATIVE ASSESSMENT - “assessment department of a chemical/pharmaceutical
for learning” company, helping to develop new and
▸ SUMMATIVE ASSESSMENT - “assessment improved products for the diagnosis and
of learning” treatment of disease
▸ DIAGNOSTIC ASSESSMENT - given prior
to instructions ▹ SECTION HEAD
▸gained experience at the bench and
ASSESSMENT TOOLS produce superior-quality work promoted to
● Written tests the supervisory level
● Reflection paper ▸responsible for making sure the laboratory
● Portfolios work assignment are completed
● Performance task ▸ arranging work schedules and managing
● Oral examination and Presentations personnel
● Rubrics ▸ usually reports to the laboratory manager
BENEFITS OF CERTIFICATION
higher wages for employees in the form of
bonuses, education assistance or higher
salary
LOCAL EXAMINATION
Blood Histotechnology
Banking/Transfusion
CPD COUNCIL
CPD ACTIVITIES
Formal learning
Non-formal learning
Informal learning
Self-directed learning
▷ HEMATOLOGY SECTION
DIABETES, LIVER DISEASE OR ▸the cellular elements of the blood such as
MALNUTRITION RBCs, WBCs, and platelets are enumerated
and classified.
FBS Fasting blood sugar
▸ BONE MARROW SAMPLES are being ▸Isolation, and identification of bacteria
studied in this section (Aerobes & anaerobes) and fungi using
varied culture media and different
biochemical tests.
EXAMINATIONS
▸Antimicrobial susceptibility testing
CBC Complete blood count
CULTURE AND SENSITIVITY
WBC differential
GRAM STAINING & AFB TESTS
count
PREPARATION OF CULTURE MEDIA
HEMOGLOBIN Rule out anemia
AND STAINS
HEMATOCRIT Haemaglobin level
INFECTION CONTROL
and red cell count
TERTIARY 60 sq m
SERVICE CAPABILITIES
PRIMARY ● CBC
● Urinalysis
● Fecalysis
● Blood typing
● Platelet count
FUNCTIONS OF NRL
IX. OCCUPATIONAL RISK
Referral services
PHARYNGITIS
▸CAUSATIVE AGENTS - Streptococcus
pyogenes Group A
▸SYMPTOMS - Swollen and reddish throat,
enlarged tonsils
CELLULITIS
▸noncotagious bacterial infection of skin
and or tissues beneath the skin
▸ SYMPTOMS - Swelling and redness of the
affected area
▸ CAUSATIVE AGENTS - Streptococcus,
Staphylococcus, Clostridium
CONJUNCTIVITIS
▸ inflammation of the conjunctiva
▸ CAUSATIVE AGENTS - Streptococcus,
Staphylococcus, Adenovirus
▸ MODE OF TRANSMISSION - Splash of X. BASIC CONCEPTS ON LABORATORY
infectious materials to the eyes, Transfer of BIOSAFETY AND BIOSECURITY
microorganisms to the eyes by
contaminated fingers BIOSAFETY
▸the containment principles, technologies, ▸ Effective treatment and preventive
and practices that are implemented to measures are present
prevent unintentional exposure to ▸ Moderate risk of transmission
pathogens and toxins or their accidental ▸ HIGH individual risk and LIMITED TO
release MODERATE community risk
▸ Protects PEOPLE from GERMS
RISK GROUP 4
BIOSECURITY ▸ Microorganisms cause
▸ the protection, control, accountability for LIFE-THREATENING DISEASES
valuable biological material within ▸ Treatment and preventive measures are
laboratotories in order to prevent their not available
unauthorized access, lost, theft, misuse, ▸ Readily transmissible
diversion or intentional release ▸HIGH individual and community risk
▸ Protects GERMS from PEOPLE
BIOSAFETY LEVELS
“ALL LABORATORY REGARDLESS OF
ORGANIZATIONS IN THE FIELD OF
BIOSAFETY THEIR BIOSAFETY LEVEL MUST FOLLOW
BASIC GOOD LABORATORY PRACTICES”
American Biological Safety Accreditation
(ABSA) BIOSAFETY LEVEL 1
▸Non-pathogenic microorganisms
Asia-Pacific Biosafety Association (APBA)
▸Standard practices
European Biological Safety Association ▸ PPE
(EBSA) ▸ REQUIRED: Lab bench and sink
● Legislative bodies
● International societies
APO
▸ Accredited Professional Organization
AIPO
▸ Accredited Integrated Professional
Organization
▸ Professional society accredited by the PRC
and the PRB
RISK
PHISMETH SEAL
▸ likelihood and consequences of an
3 CIRCLES
undesired event specific to a certain hazard
▸ continuous active involvement of Luzon,
or threat
visayas, Mindanao
LAUREL
LIKELIHOOD
▸nature and the continuation of life every
▸ factors that affect whether or not the
year
indecent happens
GREEN LETTERS
▸color of health
CONSEQUENCES
5 BUBBLES FROM TEST TUBE
▸ factors that affect the severity of the
▸5 objectives embodied in the constitution
accident
15 INTERCONNECTED MOLECULES
OUTSIDE A TEST TUBE
▸the unity of 15 board schools exploring RISK ASSESSMENT - an initial step in
various possibilities and aiming towards the implementing a biorisk management process
integral growth and holistic development
● Define the situation - identify the hazards
MICROSCOPE and risks
▸ medical laboratory science
XII. BASIC CONCEPTS OF BIORISK ● Define the risks - review of inside and
MANAGEMENT outside the laboratory may be exposed to
BIORISK the hazards
▸ Is the risk associated with biological ● Characterize the risks - compare the
toxins or infectious agents
6. Evaluate and refine performance
likelihood
indicators
● Determine if risks are acceptable or not -
the process of evaluating the biorisk
MITIGATION PROCEDURES
MOST MOST
DIFFICULT EFFECTIVE
ELIMINATION
SUBSTITUTION
ENGINEERING CONTROLS
ADMINISTRATIVE CONTROLS
PPE
EASIEST LEAST
EFFECTIVE
PERFORMANCE EVALUATION
▸ the re-evaluation of the overall mitigation
strategy
COMPONENTS OF PERFORMANCE
EVALUATION
1. CONTROL
▸ processes, procedures, structures, and
responsibilities to manage biorisk
2. ASSURANCE
▸ systematic process of checking the system
through audits and inspection
3. IMPROVEMENT
▸ setting and achieving Biorisk management
goals based on internal and external
feedback
PERFORMANCE EVALUATION
PROCEDURES
1. Identify issues
2. Define outcomes and indicators, and
metrics
3. Define activities indicators and
metrics XIII. PROFESSIONAL ETHICS
4. Collect data
5. Provide findings PROFESSIONAL ETHICS
▸ determine the morally accepted behavior ▸ indicates that a practitioner should act in
of individual in the workplace the best interest of the patient
Necessary in maintaining a healthy and
productive work environment 3. NONMALEFICENCE
▸ guides an individual in dealing with issues ▸ provides that evil or harm should not be
and conflicts in the workplace inflicted either on oneself or on others
▸ binds professions more tightly together
around shared values 4. JUSTICE
▸ concerned with the distribution of scarce
OBJECTIVES OF PROFESSIONAL ETHICS health resources and decisions about who
1. Perform duties and responsibilities gets what treatment in terms of fairness and
objectively in accordance with equality.
relevant standards & guidelines
2. Serve in a lawful and honest manner, 5. RESPECT FOR DIGNITY
while maintaining high standards of ▸ provides for all the necessary means of
conduct and character and not care and huge regard for the person or the
engage in acts discreditable to the patient, and needed information to make a
professions. relevant decisions
3. Maintain the privacy and
confidentiality of information 6. TRUTHFULNESS AND HONESTY
obtained in the course of duty ▸ dedication of a person to his job and is
unless disclosure is required by reflective of being honest and concerned
legal authority. Such information
should not be used for personal 7. STEWARDSHIP
benefit or released to inappropriate ▸ refers to the expression of one’s
parties responsibility to nurture and cultivate what
4. Maintain competency in respective has been entrusted to him.
fields and agree to undertake only
those activities one can reasonably VALUES OF A MEDICAL TECHNOLOGIST
expect to complete with 1. The medical technologist is
professional competence responsible for providing accurate
5. Perform tasks with full confidence, and reliable test results
absolute reliability, and accuracy 2. Commitment to provide prompt and
6. Be dedicated to using clinical professional service is important in
laboratory science to promote life efficient healthcare delivery
and for the benefit of mankind. 3. The obligation to protect the
confidentiality of the results and
MORAL PRINCIPLES IN MEDICAL information is a sign of respect for
TECHNOLOGY ETHICS the right of a patient to privacy.
RADIOCATIVE WASTE
▸wastes exposed to radionuclides including
radioactive diagnostic materials or
radiotherapeutic materials
▸EXAMPLES: Co, Tc, I, Ir
GENERAL WASTE PRACTICES THAT SHOULD BE
OFFSITE TRANSPORT
OBSERVED ( IN THE IMPLEMENTATION
OF COLOR CODING SYSTEM) ▸ only accredited DENR transporter and
official waste collectors are allowed to
1. Highly infectious waste must be transport wastes from the healt care facility
disinfected at source to a TREATMENT/STORAGE/DISPOSAL
FACILITY (TSD) or the Final Disposal site.
2. Anatomical waste should be
disposed through burial or
cremation TREATMENT OF HEALTHCARE WASTE
▸changing biological and chemical character
3. Pathological waste must be of waste to minimize potential to cause
refrigerated if not collected or harm.
treated within 24 hours
TREATMENT OF HEALTHCARE WASTE
4. Chemical and Pharmaceutical
waste shall be segregated and PYROLYSIS Is the thermal
collected separately decomposition of
healthcare wastes
5. Radioactive should be decayed to
background radiation levels AUTOCLAVE Is the use of steam
sterilization ro render
6. All waste bins must be covered waste harmless
to prevent cross contamination An efficient wet thermal
disinfection process
7. Aerosols containers can be
collected with the general waste MICROWAVE Is a technology that
typically incorporates
some type of size
COLLECTION AND TRANSPORT
reduction device
9. PD NO 1586 “Environment
Impact Statement (EIS) System”
(1978)