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Analysis of Honey
Analysis of Honey
Project on
ANANLYSIS OF HONEY
In
CHEMISTRY
Submitted By
HARSHITA SINGH
P.G.T.
GayatriVidyapeeth
HARIDWAR-249411
2023-2024
CERTIFICATE
P.G.T. CHEMISTRY
GAYATRI VIDYAPEETH
SHANTIKUNJ, HARIDWAR
CERTIFICATE
PRINCIPAL
GAYATRI VIDYAPEETH
SHANTIKUNJ, HARIDWAR
INDEX
Introduction
Uses of honey
Conclusion
Reference
INTRODUCTION
Honey, a natural substance produced by honey bees, is sweet and has a
syrupy consistency. It has a beautiful golden-yellow hue and a complex
composition with various physiochemical and antioxidant properties.
Essentially, honey is a highly concentrated sugar solution, comprising a
mixture of carbohydrates. Along with carbohydrates and water, honey
contains proteins, enzymes, organic acids, vitamins, lipids, phenolic
acids, flavonoids, minerals, and other beneficial compounds in small
quantities. Throughout history, humans have widely consumed honey,
utilizing it in the food, medicine, and beverage industries. Notably,
honey possesses antibacterial and antifungal properties, further
enhancing its value. The medicinal benefits of honey have been
acknowledged and recorded in different ancient societies like India,
Egypt, and Greece. All ancient Ayurvedic scriptures highlight the value
of honey. Honey is gaining much attention in the modern world as well.
Honey, often referred to as “liquid gold,” has been a cherished natural
sweetener and medicinal substance for thousands of years. Its historical
significance is evident from ancient texts, archaeological findings, and
folklore from various cultures around the world. Beyond its delightful
taste, honey’s complex composition and myriad health benefits have
intrigued scientists and researchers, prompting extensive investigations
into its properties and applications. This project aims to delve into the
multifaceted world of honey, exploring its chemical composition,
physical properties, medicinal value, and potential applications in various
fields.
Honey In Traditional
System
Human use of honey is traced to some 8000 years ago as depicted by
Stone Age paintings.It has been a cornerstone in traditional medicine,
culinary practices, religious rituals, and cultural traditions for millennia.
Its rich history and multifaceted uses across various civilizations
underscore its significance in human society. This section delves into the
diverse roles honey has played in traditional systems around the world.
Traditional Medicine
4. Unani Medicine
Therapeutic Uses: Honey, or “Asal” in Unani medicine, is used for
its detoxifying and rejuvenating properties. It is believed to help in
maintaining overall health and treating a variety of ailments from
digestive issues to cardiovascular diseases.
Culinary practices
1. Judaism
Rosh Hashanah: Honey is consumed during the Jewish New Year
(Rosh Hashanah) to symbolize the hope for a sweet new year.
Apples dipped in honey are a common tradition.
Biblical References: Honey is frequently mentioned in the Bible as
a symbol of abundance and prosperity.
2. Islam
Prophetic Medicine: Honey is highly esteemed in Islamic tradition,
often mentioned in the Quran and Hadiths for its healing
properties. Prophet Muhammad recommended honey for health
and healing.
Cultural Practices: Honey is used in various cultural rituals and
celebrations across the Muslim world
3. Hinduism
Religious Offerings: Honey is one of the Panchamrita (five nectars)
used in Hindu religious ceremonies. It is offered to deities andused in
various rituals.
Symbol of Purity and Prosperity: Honey symbolizes purity,
knowledge, and prosperity in Hindu culture.
Chemical composition of
Honey
Honey is a complex mixture and presents very great Variations in
composition and characteristics due to its Geographical and botanical
origin, its main features Depending on the floral origin or the nectar
foraged by Bees. The composition and quality of honey also Depend on
several environmental factors during Production such as weather and
humidity inside the Hive, nectar conditions and treatment of honey
during Extraction and storage. The composition of honey varies With the
feeding of the bees. Honey has been reported to Contain more than 180
substances and is considered as An important part of traditional
medicine.
Chemical composition
The composition of honey basically varies to the floral Source, but
seasonal, environmental factors and Processing conditions are also
important.
1. Carbohydrate:
Sugar in honey is not a single species, but consists of Three kinds of
sugar. These are the fruit sugar (fructose), Which has among the
highest (41%), grape sugar (glucose), which has about 34% of
ordinary sugar (sucrose) which is between 1 and 2%. The ratio of One
type of sugar to other depends of the source, i.e., Flower pasture, and
to some extent on enzyme invertase, Which breaks down regular
sugar in grape and fruit. This enzyme is located in the flower from
which the Bees collect nectar, but it is also present in the bee’s Body .
2. Amino acids and proteins
Proteins come in honey from nectar and pollen as a Integral parts of
plants. Proteins in honey may be in the Form of a very complex
structure or in the form of Simple compounds, i.e., amino acids. The
content of Amino acids and proteins is relatively small, at the most
0.7 %. Honey contains almost all physiologically Important amino
acids. The main amino acid is proline Is a measure of honey ripeness.
The proline content of Normal honeys should be more than 200
mg/kg. Values Below 180 mg/kg mean that the honey is probably
Adulterated by sugar addition.
4. HMF (HydroxyMethylFurfuraldehyde)
HMF is a six-carbon heterocyclic organic compound Containing both
aldehyde and alcohol (hydroxymethyl) Functional groups. The ring of
thestructure is Centered on furan moieties, whereas the two functional
Groups, i.e., formyl and hydroxy-methyl groups, are Linked at the
second and fifth positions, respectively HMF is a solid, yellow
substance that hasa Low melting point but is highly soluble in water.
6. Acidity and pH
Honey contains a number Of acids which include Amino acids (0.05-
0.1%) And organic acids (0.57%, Range: 0.17-1.17%).A number of
organic acids Are known to occur in Honey, including acetic, Butyric,
citric, formic, Gluconic, lactic, malic, Pyroglutamic, and succinic. The
major organic acid is Gluconic acid. Gluconic Acid is produced in
honey By the action of the Enzyme glucose-oxidase On glucose.
Average pH of honey is 3.9 (with a typical range of 3.4 To 6.1).
7. Water
The water content of honey (water-in-honey) is the Quality aspect that
determines the ability of honey to Remain fresh and to avoid spoilage by
yeast Fermentation. Raw honey can have a water-in-honey Content of
less than 14% and the lower the water Content the higher the perceived
value of the honey. It is internationally recognized that good Quality
honey should be processed at less than 20% Water content. Low water
content is desirable Because honey may begin to ferment and lose its
Fresh quality if the water-in-honey is greater than 20%.
Analysis of components in
Different Types of honey
Analyzing the mineral content of various types of honey provides
valuable insights into their nutritional profiles and potential health
benefits. Different types of honey sourced from diverse floral origins and
geographical locations may exhibit distinct mineral compositions,
influenced by factors such as soil composition, climate, and floral
sources. Conducting a comprehensive analysis involves determining the
concentrations of essential minerals such as potassium, calcium,
magnesium, phosphorus, sodium, iron, zinc, and manganese, among
others.
Requirements:-
1. APPARATUS
Test Tube
Test Tube Stand
Test Tube
Holder Burner
Water Bath
2. CHEMICALS
Fehling Solution A Fehling Solution B
Molish’s Reagent. Ammonium Oxalate Solution
Iodine Solution. Benedict ‘S Reagent
Ammonium Chloride Solution Tollen’s Reagent
Ammonium Phosphate Conc.Nitric Acid
Procedure
2. Tollen`s test:
2-3 ml of aqueous solution of honey is Taken in a test tube. 2-
3ml of Tollen`s Reagent is added. The test tube is kept In a boiling
water bath for about ten Minutes. A shining silver mirror
Indicates the presence of reducing Carbohydrates.
3. Molisch’s test:
To the carbohydrate solution in a Test tube, 2 drops of α-
naphthol solution is added Followed by Con. H2SO4 along the
side of the test tube, Violet ring develops at the junction of two
liquids.
4. Benedict’s test:
To the carbohydrate solution in a test tube, Benedict’s reagent is
added, heated in a boiling water bath, Formation of orange or
red precipitate indicates the presence of Reducing sugar.
5. Iodine test:
To the carbohydrate solution, 2 drops of iodine Solution added,
formation of blue-black colour indicates the Presence of
polysaccharides.
Observation Table
S.no. Tests Observation Inference
1. Test for potassium:- Yellow ppt is Potassium is absent all samples
Honey +picric observed
acid
solution
2. Test for White ppt is Calcium is absent in all samples
calcium:- observed
Honey +
NH4Cl+
NH4OH+
(NH4)2 C2O4
3. Test for White ppt is Magnesium is absent in all
magnesium:- observed samples
Honey +
NH4Cl+
NH4OH+
(NH4)2CO3+
Na2HPO4
4. Fehling’s Test :- Brownish red ppt is Patanjali – dark
Honey solution + observed brown ppt Dabour-
equalvolume of reddish brown ppt
Fehling’s Apis- brownish ppt
solution A and It contains reducing sugar
Fehling’s solution B,
Heat inwater bath
for 5-10 min.
5. Tollen’s Test:- Shinning silver Shinning silver mirror
mirror observed observed,It
contain reducing
carbohydrates
Honey + 2-3 ml
Tollen’sreagent, heat
in water
bath for 5 minutes
6. Molisch’s Test:- Violet ring develops It contains reducing
Honey+ Molisch’s at the junction of carbohydrates
reagent + Conc. two liquids
H2SO4
from side of test tube
7. Benedict’s Test:- Orange or red ppt Brownish ppt observed in all
Mix equal volume of observed samples
Benedict’ s reagent Or Brown
and honey solution in ppt is
observed
test tube, heat in
boiling water bath
for 5
minutes
8. Iodine Test:- Blue-black colour It doesn’t turn blue or black
Honey+ iodine indicates the It means it does not
Solution presence of Adulterated withstarch or
polysaccharides flour
Results
Potassium is absent.
Calcium is absent
Magnesium is absent
It contains reducing sugar
It contains reducing carbohydrates.
It is not Adulterated with starch or flour.
Uses of Honey
Honey, known for its delectable sweetness and unique properties, has a
wide range of uses beyond its medicinal benefits. This versatile natural
product plays significant roles in culinary arts, skincare, agriculture, and
even environmental conservation. Below is a comprehensive overview of
honey’s diverse applications outside the medicinal realm:
Culinary Uses
Agriculture
Environmental Uses
https://www.researchgate.net/publication/313785968_He
alth_Benefits_and_Traditional_Uses_of_Honey_A_Review