kolichestvo 8

T, grad 95 60 72
2-x PCR mix 2,5(AB) 10 80 15'' 30'' 30''
primers 1.5 12 50 cycles
Pol (Therm) 0.2 1.6
SYBR 0.2 1.6
Mg 0.4 3.2 melt range 75-95
H2O 5.7 45.6 increment 0.2
vsego 18 144 hold 0.1
DNA, mkl 2

15 16 17 18 19 20 ntc
1 2 3 4 5 6 7 есть надо

A tnf a 61.5 61.6

B HBEGF 61.4 61.5

C PDGFR-A 61.3 60.7

D SDHA1 61 60.6

E SDHA1 60.6 60.6

F SDHA 2 60.3 60

G SDHA 2 60.1 60

H IGF 1 60 60