CELL LINEAGE TYPE OF CELL CELL MORPHOLOGY

MYELOID
BLAST *One or more nucleoli
*Fine-grained Chromatin Pattern
*Auer Rods present in Cytoplasmic Space
* High N/C ratio with darker cytoplasmic
edge
*Determined by MPO & CD Markers

LYMPHOID NUCLEOLI

*One or more prominent nucleoli

*Fine-grained Chromatin Pattern
* High N/C ratio with darker cytoplasmic
edge
* Determined by CD Markers

MONOBLAST MONOCYTIC

*Fine Chromatin Nucleus

*Small Cytoplasmic Vacuoles
* One or two prominent nucleoli
*Larger than Myeloid & Lymphoblast

PROMYELOCYTE BRIGHTENING EDGE

GRANULOCYTIC
*Large cell with a round or oval shape
*Eccentric nucleus
*one or more nucleoli
(NEUTROPHILIC SERIES) *Dark blue to purple cytoplasm
*single brightening at the edge of the
nucleus (golgi zone)
*dark blue to pink colored granules all over
the cell
 MYELOCYTE

*Less granulated; granules lie over the

nucleus to a lesser extent.
*Smaller than Promyelocyte
*Eccentric Nucleus
*Light Blue Cytoplasm

METAMYELOCYTE

*Kidney-shaped Nucleus
*Coarse Chromatin Pattern
*Pale Blue Cytoplasm
*Reduced granulation than Myelocyte

BAND OR STAB

*Horse-shoe or Telephone Appearance

with one or more nicks
*Pinkish Cytoplasm with numerous
Purple-reddish granules or little to No NICKS
coarse granules present

NEUTROPHIL/SEGMENTED

*Has 2 or more lobes connected by thin

filaments
*Pink cytoplasm with numerous fine
purplish-red granules that contain anti-
bacterial & protein degrading
substances.
 NEUTROPHIL
(TOXIC GRANULATION)

*Large Purple to Dark Blue granules with

increase staining density than normal
neutrophil.
*Granules lie all over the cell

GRANULOCYTIC MYELOCYTE

*Round or Oval Nucleus lying

(EOSINOPHILIC SERIES) eccentrically
* Light Purple or Pink cytoplasm
* No Basophilic Granules present
*No cell distinction made between
Neutrophil, Eosinophil, and Basophil

METAMYELOCYTE

*Kidney-shaped appearance
*Coarse Chromatin Pattern
*No Basophilic Granules present
*No cell distinction made between
Neutrophil, Eosinophil, and Basophil
 BAND

*Single curved unsegmented nucleus

(Horse-shoe like appearance)
*Thick Pink granules present in
cytoplasm
*Eosinophilic Bands are usually counted
as Eosinophilic Granulocytes

EOSINOPHIL/SEGMENTED

*Has 2 or 3 nuclear lobes

*Packed Pink granules present in
cytoplasm
*Recruitment of Eosinophils occurs
under the influenced of IL-5.

GRANULOCYTIC BASOPHIL/SEGMENTED

*9-14 μm in diameter
*Contains large Dark Purple granules
(BASOPHILIC SERIES) that lie all over the cell
*Granules contain histamine and other
inflammatory mediators
*Activates by binding to IgE and or
complement

”LEACHED/DEGRANULATED BASOPHIL”
 PROLYMPHOCYTE
NUCLEOLI
LYMPHOCYTIC SERIES *Large precursor of normal lymphocyte
*One or two large highly visible nucleoli
*Light gray or light blue cytoplasm with
darker regions around the borders of
the cell
*Relatively more frequent in neonates

LYMPHOCYTE

*Slightly larger than normal RBC

*Little to No Dark Blue cytoplasm,
usually with No granules
*Coarse Nucleus
*No distinctions between T and B
Lymphocytes

LARGE GRANULAR LYMPHOCYTE

*Large lymphocytes that sometimes

may appear as Reactive Lymphocytes
*Thick granules present in cytoplasm
*These are NK or T cells and is about
5-10% of lymphocyte population
*Abnormal count: 20% of cells is present
for an extended period of 6 months
 PLASMA CELL

*Round eccentric nucleus

*Coarse Chromatin Pattern with bluish
to dark blue cytoplasm
*Cytoplasmic space may appear as
“Fanned-like” with brightening white
perinuclear court
*Are fully mature and specialized B
lymphocytes that produce antibodies

REACTIVE/ATYPICAL
LYMPHOCYTE

*Large lymphocyte with high cytoplasmic

ratio than its nucleus
*Nucleus is larger than the normal
lymphocyte
*Condensed chromatin pattern
*Deeply basophilic cytoplasm
*Usually has a “scalloped-like” shape due
to squeezing of surrounding cells; giving
them also an irregular shape
*Sometimes round or oval in shape, or
indented with a typical stretched
appearance.
*Immune response to antigen could lead
to transformation of cells into
Plasmacytoid.
PLASMACYTOID
 PROMONOCYTE
MONOCYTIC SERIES *Darker blue to gray cytoplasm
*Nucleus has “thin-lines” created by the
lobes of the nucleus which then may
look like a “Brain” THIN-LINES OF
NUCLEAR LOBES

MONOCYTE

*The largest leukocytes among all

normal cells
*Grayish cytoplasm
*May have vacuoles at times due to
metabolic activity of the cell
*Various shapes are seen

MACROPHAGE

*Nucleus is usually ovoid in shape

*Cytoplasm may appear as a
“big pancake” due to vacuolation
*May have granules that are basophilic
in nature
*Not normally seen in Peripheral Blood
*They are highly phagocytic and serve as
an “Antigen-presenting” cell that links
between the nonspecific and specific
immune system

ERYTHROCYTIC SERIES
They are the smallest hematological
cells in the blood and are 2-3 μm in
size. These granules contain
vasoactive substances that support
hemostasis.
PROERYTHROBLAST Border “Ears”
(RUBRIBLAST/PRONORMOBLAST)
*The youngest erythroid progenitors
*Nucleus is eccentrically located
*Fine chromatin pattern, almost
perfectly round
*Cytoplasm is deeply basophilic with
dark border
*May have one-two “ears” in its border
BASOPHILIC ERYTHROBLAST
*Has a dark or dark blue nucleus &
cytoplasm
* Slightly Coarser Nucleus
*Sometimes difficult to distinguish from
other blasts
POLYCHROMATIC ERYTHROBLAST
*Round Nucleus with Patchy character
*Nucleus is smaller and relatively more
cytoplasm
*Dark blue to Purple in color
 ORTHOCHROMATIC ERYTHROBLAST

*Nucleus is round and Dark

*Uniform Chromatin Pattern
*Cytoplasm is grayish blue to purple
*Nucleus may be expelled once it fully
matures

POLYCHROMATIC ERYTHROCYTE
(RETICULOCYTE)

*Has “networks of filaments” due to

ribosomal remnants
*Filaments may appear as gray or blue
when stained by supravital stain.
*Their presence in the peripheral blood
suggest possible anemic state

ERYTHROCYTE/NORMOCYTE

*6-8um in size, similar to the nucleus of

lymphocyte
*Mature RBC has slight “Central Pallor”
*Pinkish in color when stained

PLATELET/THROMBOCYTES
*Smallest cell in the blood (2-3um)
THROMBOCYTIC SERIES *Purplish to Pink in color
*Contain vasoactive substance for
hemostasis