Code: L37RS1051-10

Designation: NCTC 12981 / ATCC© 25923

 ANALYSIS CERTIFICATE / TECHNICAL SHEET
Lot Number: 01305102
Description: Staphylococcus aueus NCTC 12981 / ATCC© 25923
Product Code: L37RS1051-10
Expiry: 2025.09 (Important Note: Use within 9 months of opening).
A first generation derivate of: NCTC No: 12981
Number of CFU: 106 to 109
Source lot: 6a white
ACDP hazard group: 2
Storage: - 20oC to + 8oC

TEST REPORT ON REFERENCE STRAINS QUALITY CONTROL SAMPLES FROM: Lot 01305102

Issue date: 2023.08.07

Testing date: 2023.07.03
Gram stain: Gram positive cocci
Catalase: Positive
Oxidase: Negative
Chromogenic Agar: Positive
DNAse: Positive
Coagulase Protein A: Positive

Organisms derived from NCPF and NCTC cultures

ATCCR is the registered trade mark of the American Type Culture Collection
NCTC – National Collection of Type Cultures
NCPF – National Collection of Pathogenic Fungi
ACDP – Advisory Committee on Dangerous Pathogenes

INSTRUCTIONS OF USE
Reference Strains Quality Control Samples (Discs) are first generation derivatives traceable to recognised culture collection strains. They can be
used to prepare working stock cultures for the control of microbiological test methods. (see Limitations). Reference Strains Quality Control
Samples (Discs) are freeze-dried microbial preparations manufactured from NCTC® (National Collection of Type Cultures) and NCPF®
(National Collection of Pathogenic Fungi) cultures. The viability of the organisms is stabilised by the unique formulation of the disc.

Precautions & storage

• Reference Strains Quality Control Samples (Discs) contain pathogenic organisms and should only be used in appropriately equipped
laboratories by microbiologists or laboratory trained personnel under appropriate supervision.
• Store at the temperature indicated on the label. Loss of viability may occur if the discs are left above this temperature range for longer than
necessary.
• Allow the vial to reach room temperature before opening and replace the cap on each vial immediately after a disc has been removed.
Moisture in air entering the vial will cause a reduction in the number of viable organisms, eventually leading to complete loss of viability.
• Do not use discs that are past their expiry date. These may show a loss of viability.
Removing the disc from the vial: A disc is easily removed from the vial by using sterile forceps or a sterile 10μl inoculating loop.

Suggestion for use on solid media: Place a disc on appropriate solid medium. Allow the disc to soften for 10 – 15 minutes. The plate may be
placed in an incubator to accelerate the process. Spread the softened disc around the plate and incubate under optimum conditions for the
strain.

Suggestion for use in liquid media: Place the disc in 1-10ml of the appropriate broth. Avoiding the production of aerosols, shake the broth
gently to dissolve the disc and incubate under optimum conditions. By experimentation you will be able to find the best dilution for your own
application.

Limitations: Repeated subculture can cause the characteristics of a strain to change. Ideally, no more than four passages from the Reference
Strains Quality Control Samples (Discs) working stock culture should be made.

Breakages, spillage & disposal

• All exposed discs, contaminated packaging and broken glassware should be placed in a suitable container and either incinerated or
autoclaved at 121°C for 30 minutes.
• Unbroken vials may be removed with forceps, washed in a suitable bactericidal solution, rinsed, dried and retained for further use.
• All contaminated surfaces must be disinfected with a suitable bactericidal solution.

SYMBOLS

Code number Expiration date Lot of Manufacturing Warning, read enclosed documents Read the instructions Biological Risk

SC Tody Laboratories INT. SRL

Address: Vadul Moldovei Street, no 22, Sector 1, Bucharest, Romania
Tel.: +4021 269 00 00 Fax: +4021 269 06 41
www.todylaboratories.com