Name of all lab partners:

Lab Section:

Symbiosis in the Soil, Session 2

Group Lab Assignment
10 points
1. (0 pts.) What was your group’s research hypothesis? If you missed any points from your
Symbiosis, Session 1 group assignment on your hypothesis, revise your research
hypothesis as suggested.

2. (1 pt.) Insert a graph for number of nodules on roots of plants inoculated with rhizobia or
water without nitrogen, and those plants inoculated with rhizobia or water with nitrogen.
Choose which type of graph you think would best represent this type of data, but make
sure the axes are labeled, there is a proper title, a proper legend (if applicable), and there
is a title.

3. (3 pt.) Insert graphs in the form of boxplot(s), scatterplot(s), or bar graph(s) that you
constructed for the variable(s) that your group chose to study. Again, choose which
format for each variable you find appropriate. Make sure that variable(s) other than
nodule number that are relevant to your group’s hypothesis are on the Y-axis. Construct a
separate graph for each variable relevant to your group’s hypothesis. You may include as
many graphs as necessary; this is wholly dependent on your hypothesis. For each graph
make sure the axes are labeled, there is a proper title, a proper legend (if applicable), and
there is a title.

4. (3 pts.) Perform a t-test comparing nodule number in plants with nitrogen and plants
without nitrogen. Then do t-tests for each of the other variables relevant to your group’s
hypothesis, comparing each of these variables for plants with or without nitrogen.
Generate a table indicating the p-values that you obtained for each pairwise comparison
for each dependent variable relevant to your group’s hypothesis.

5. (1 pt.) Is your research hypothesis supported by this data?

a. It’s possible that you aren’t able to fully test your hypothesis based on the results
(for example, if the comparison with the experimental control didn’t yield
expected results).
i. If you can’t test your hypothesis, explain why.

Bio 1124: Introduction to Organismal Biology

Spring 2024
Name of all lab partners:
Lab Section:
b. It’s also possible that your hypothesis is partially, but not fully supported (for
example, if your hypothesis is supported for nodule number, but not for the other
variable(s) you analyzed).
i. If you can test your hypothesis, explain why it is supported, partially
supported or not supported by this data.

6. (2 pts.) What conclusions can you make? Refer back to your biological rationale and
explain the implications of these results. What do your results tell you about legume-
rhizobia symbiosis in plants that had no nitrogen or exogenous nitrogen added? Refer
back to references used in your biological rationale from Session 1, or any new
references that you find to support your conclusions. Be sure to include references (you
must include at least one reference other than the lab manual) and cite them.

References (1 pt. off if not included or incorrect)

If you reference sources from the lab manual, internet, the primary literature, or your textbook,
you should cite these references. Please use the following examples to format your citations:

Citation Examples
In text:
1 author: (Siegert, 2023)
2 authors: (Siegert and Robinson, 2023)
3 or more authors: (Siegert et al., 2023)
Webpage: the first few words of the reference list entry (usually the title) and the year. Use
double quotation marks around the title or abbreviated title.: ("All 33 Chile Miners," 2010).
Note: Use the full title of the web page if it is short for the parenthetical citation.

Works Cited
For the lab manual chapter:
Siegert, K. Chapter 10 Symbiosis in the City, Session 2. Biology 1124 Lab Manual, Spring
2024, 2024.

For articles:
Hyperlink with text: Author name. Title of article. Date published.

For a Page on a Website:

"Athlete's Foot - Topic Overview." WebMD, 25 Sept. 2014, www.webmd.com/skin-problems-
and-treatments/tc/athletes-foot-topic-overview.

For the textbook or another book:

Gillespie, Paula, and Neal Lerner. The Allyn and Bacon Guide to Peer Tutoring. Allyn and
Bacon, 2000.

Bio 1124: Introduction to Organismal Biology

Spring 2024