Shrikant Sir

Chemistry Structure of substances

Organic Inorganic Biochemistry Physical Analytical

Pharmaceutical Chemistry
Structure of Drugs/ Pharmaceutical

Organic Inorganic Biochemistry Analytical

Physical

All used in Pharmacy add prefix Pharmaceutical

  Material Science
 Physics
Pharmacy  Chemistry
 Research  Biology
 Medicine/Drug  Geology
 Drug Actions Analytical Chemistry  Environmental
 Clinical Science
Examination  Agriculture
 Toxicology
Investigation  Forensics
Inspection  Martial Science
Survey  Engineering
Scanning
 Analysis Provide Tools and Methods to provide answer of four word for Substance

⧃ What ?
⧃ How Much?
⧃ Arrangement / Structure /Form?
⧃ Where?
GPAT/ NIPER
 Pharmaceutical Analysis Syllabus

How Can Cover ?

Section 6
Section 5
Section 4
Section 3
Section 2
Section 1
 Pharmaceutical Analysis Syllabus

Section 1
⧃ Introduction Pharmaceutical Analysis
⧃ Importance of quality control in pharmacy
⧃ Accuracy Precision Errors and Calibrations
 Section 2
⧃ Electromagnetic Radiations
⧃ General principles of spectroscopy
⧃ Ultraviolet-visible Spectroscopy
⧃ Infrared Spectroscopy
⧃ Nuclear Magnetic Resonance Spectroscopy
⧃ Mass spectroscopy
⧃ Electron Spin Resonance Spectroscopy
⧃ Raman Spectroscopy
⧃ Spectroflorimetry
⧃ Nephelometry & Turbidimetry
⧃ Flame photometry
⧃ Atomic absorption spectrometry
⧃ Atomic emission spectrometry
⧃ X Ray Methods
 Section 3
⧃ Chromatography

Section 4
⧃ Acid-base titrations
⧃ Gravimetry
⧃ Non-aqueous titrations
⧃ Oxidation-reduction titrations
⧃ Precipitation titrations
⧃ Complexometric titrations
⧃ Miscellaneous Titrations
 Section 5
⧃ Potentiometry
⧃ Polarography
⧃ Amperometry
⧃ Coulometry
⧃ Voltammetry
⧃ Polarimetry
Section 6
⧃ Thermal Methods
⧃ Classical Methods
⧃ Extraction Methods
⧃ Assay Methods
⧃ Miselleneous Methods
 Recommended Books
Lectures and GDC Modules are Sufficient
◊ Silverstein- Spectrometric identification of organic compounds
◊ Pavia- Introduction to Spectroscopy
◊ Y. R. Sharma -Elementary Organic Spectroscopy
◊ Chatwal Instrumental Methods of Chemical Analysis
◊ Remington's Pharmaceutical Sciences
Analytical chemistry is the branch of chemistry that deals with the analysis
of different substances.

Pharmaceuticals

Pharmaceutical Analysis

Separation Quantification Identification

 Role of Pharmaceutical Analysis
 The pharmaceutical manufacturer is responsible for ensuring the

following for each drug product

 Quality

 Purity

 Identity

 Strength
 Isolation Identification Analysis

Cinchona Bark
API- Quinine
 Pharmaceutical Analysis- Main Techniques

Titrations

Spectroscopy

Chromatography

Electroanalyis

Miselleneous
Titrations
 Spectroscopy
Absorption Spectroscopy Emission Spectroscopy

Pharma
Company 2
Pharma
Humanity
Company 1
 Chromatography

Separation

Mixture

Mobile Phase Stationary Phase Separation

Shrikant Sir
 Pharmaceutical Analysis- Main Techniques

Titrations

Spectroscopy

Chromatography

Electroanalyis

Miselleneous
 Analytical Chemistry Methods

Classical Methods Instrumental Methods

Acid test for gold Spectroscopy- UV, IR, NMR

Kastle-Meyer test Mass spectroscopy

Flame tests Chromatography
Volumetric analysis Potentiometry
Gravimetric analysis Polarography
Checking for the presence or absence Amperometry
Conductometry
 Titrations

Volumetric Titrations Gravimetric Titrations

Aqueous
Acid Base Titration
Non Aqueous
Redox Titrations
Precipitation Titration
Complexometric Titration
Potentiometric Titration
Diazotization
 Instrumental Methods

Spectroscopy Electroanalytical Miscellaneous Chromatography

UV -Visible Potentiometry Thermal Methods
IR Amperometry Polarimetry Hyphenated
technique
NMR Voltammetry RIA
Chromatography
Mass Polarography ELISA
ESR Coulometry Karl Fisher Spectroscopy
AAS & AES Chronopotentiometry Kjeldahl Method
X -Ray Electrogravimetry
Flame Phtometry
 Types of Assay/Test

Chemical Assay Biological Assay

Spectroflurimetry Bioassay
Spectrophotometric Assay Immunological Assay
Chromatography Microbiological Assay
Non Instrumental Methods
 Basic Principal of Instrumental Analytical Methods

Reading
Analytical Methods Sample Production of Signals Device

Change in Physical and Chemical Properties

 How can Work Instrumental Analytical Methods

Production of Signal Signals produced from Sample

Transduction Transformation of signal to different Nature

Amplifications Increases the sensitivity

Presentation Display of Results

 Nature Analytical Methods

Destructive Methods Nondestructive Methods

 Sample affected Sample not affected
 Not reused Reused if required
 Permanent and irreversible
chemical changes
 Used minor Quantity
 Branches of Analytical Chemistry
1. Quantitative Analysis
⍣ Quantitative Analysis is a method of determining the absolute or relative
quantity regarding the concentration of one or more substances present
in a sample or compound.

2. Qualitative Analysis
⍣ Quality means the standard or the feature of one substance.
⍣ Yes or NO
⍣ Presence or Absence
 Target of Analytical Methods

Elemental analysis Molecular Analysis

Qualitative Quantitative
Qualitative Quantitative
 Methods of Analysis
Properties Measured Methods
Volume ◊ Volumetric Titration
Weight ◊ Gravimetric Titration
Complex Formation ◊ Complexation Titration
Precipitate Formation ◊ Precipitate Titration
Concentration in Rate Law ◊ Kinetic Methods of Analysis
Nuclear Reactions ◊ Gamma Rays Methods
Inner Shell Electronic Changes ◊ X Ray Analysis Methods
 Methods of Analysis
Properties Measured
Absorption of Radiation
Emission of Radiation
Mass to Charge Ratio
Methods
◊ Spectrophotometry [UV Visible, IR]
◊ NMR
◊ ESR
◊ X Ray
◊ Atomic Absorption Spectroscopy
◊ Colorimetric
◊ Emission spectroscopy [UV Visible, IR]
◊ Flame Photometry
◊ Fluorescence
◊ Mass Spectroscopy
 Properties Measured Methods

Scattering of radiation ◊ Turbidimetry

◊ Nephelometry
◊ Raman Spectroscopy

Refraction of Radiation ◊ Reflectometry

◊ Interferometry

Diffraction of radiation ◊ X Ray diffraction

◊ Electron Detraction Methods
 Properties Measured Methods

Rotation of radiation ◊ Polarimetry

◊ Optical Rotatory dispersion
◊ Circulars Dichroism

Electrical Potential ◊ Potentiometry

◊ Chronopotentiometry

Electrical Conductance ◊ Conductometry

Electrical Current ◊ Polarography

◊ Amperometric Titration
Physical Properties Measured Methods

Quantity of electricity ◊ Coulometry

Thermal Properties ◊ TGA

◊ Deferential Thermal Scanning

Partitions ◊ Chromatography
Adsorption
Ion Exchange
Affinity
Size Exclusion
Q.1. Kastle-Meyer test-
(a) Detection of Hb
(b) Detection of Metals
(c) Detection of Acid
(d) All
Q.2. Analysis on Biological System Known as
(a) Spectroscopy
(b) Bioassay
(c) Titrations
(d) None
Q.3. All Methods have use volume of titrant except
(a) Polarimetry
(b) Potentiometry
(c) Amperometry
(d) Voltammetry
Q.4. UV Spectroscopy responsible for
(a) Electronic transitions
(b) Vibration Transitions
(c) Spin Transitions
(d) None
Q.5. NMR Spectroscopy Used----- rays of EMR
(a) NMR
(b) UV
(c) IR
(d) Radio waves
Q.6. Principle of Chromatography and UV visible spectroscopy
(a) Absorption & Adsorption
(b) Partition & Adsorption
(c) Partition & Absorption
(d) Absorption & Partition
Q.7. Nature of sample for analytical methods
(a) Molecular
(b) Elemental
(c) Both
(d) None
Q.8. Measurement of Current
(a) Polarimetry
(b) Polarography
(c) Coulometry
(d) Potentiometry
Q.9. Choose methods that adsorption of Radiation
(a) UV
(b) IR
(c) Both
(d) None
Q.10. Measurement of Potential
(a) Polarimetry
(b) Polarography
(c) Coulometry
(d) Potentiometry
Q.11. Mass to Charge ratio measured in
(a) Flame Photometry
(b) NMR
(c) UV Absorption
(d) Mass Spectroscopy
Q.12. Measurement of rotation of radiation
(a) Polarimetry
(b) Polarography
(c) Coulometry
(d) Potentiometry
Q.13. Principle of chromatography except
(a) Absorption
(b) Partitions
(c) Affinity
(d) None
Q.14. Techniques used for Separation
(a) Polarimetry
(b) Chromatography
(c) UV Spectroscopy
(d) Potentiometry
Q.15. Principle of chromatography
(a) Absorption
(b) Partitions
(c) Affinity
(d) B &C
Q.16. Infrared analysis can be operated by
(a) Absorption
(b) Emission
(c) Both
(d) None
Shrikant Sir
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