CIRCULATING MIR‑LET7A

Facultad de Medicina y Ciencia

Carrera de Tecnología Médica
LEVELS
PREDICT FUTURE
Diagnóstico Molecular I 2024

DIAGNOSIS OF CHRONIC
THROMBOEMBOLIC
PULMONARY
HYPERTENSION
Members:Constanza Espinoza Labbé
Agustín Salvo Armijo
Teacher: Cherie Hernández and Jaime Gutierrez
NRC 82.. Section 50 Diagnóstico Molecular
INTRODUCTION Sudden blockage of an artery
(blood vessel) in the lung
Embolism Pulmonary

Incidence of 39- 115 per Is the third most common

100.000 persons- years and is cardiovascular disease
associated with serious short
and long- term

Chronic Thromboembolic Pulmonary Little-known disease that occurs due to chronic

Hipertension occlusion of the pulmonary arteries by organized
thrombi

Is rare disease
R (National Cancer Institute)
High mortality rates
(R (Salisbury, Curbelo, Arcaus, & Cáneva, 2011)
INTRODUCTION They promote degradation and
suppress its translation.

non-coding RNAs

Emerged as novel biomarkers and distinct

patterns were found to exhibit diagnostic and
miARN prognostic

Studies have proven the existence of

dys -balanced miRNA concentrations

miRNAs are stable in the circulation

Diagnosis and promising prognosis

R (Nelson & Cox, 2018)
 Regulation of gene
miARN
miR-29a expression
deregulated Inflammatory
processses and
fibrosis

Family Let-7
miR-let7a
Regulation of gene
expression
miR-720 Diagnostic Biomarker

Regulation of gene
expression
Cell growth, proliferation,
migration and invasion

R (Chirshev, Ioffe, Oberg, & Unternaehrer, 2019)

R (Huang, Yang, & Wang, 2018)
R (Graves, Sandhu, & Kingsley, 2020)
OBJECTIVE

To study miRNA patterns in patients with pulmonary embolism (PE)

and their ability to predict CTEPH
METHODS OR METHODOLOGY
Ill patient group

Patients

Control group
METHODS OR METHODOLOGY
Blood sample

standardization
process and with miR-39 Heparinase
storage at sintetic pre-traetment
-80°C

Qiazol/chlorofor
Kit miRNeasy m/plasma
serum/plasma mixture
METHODS OR METHODOLOGY
After RNA Heparinase
isolation pre-treatment

Real time PCR TaqMan Array 20 random

hsa-miR-720 Reverse patients
hsa-miR-29a
transcription sample
hsa-miR-39
cel-miR-39
RESULTS
Flow-chart of miRNA- Heatmap of TaqMan miRNA Array
screening and the study
RESULTS
volcano plot of deregulated miRNAs in plasma
RESULTS
N=20
RESULTS
N=177
CONCLUSIONS
Pulmonary embolism remains an important and main contributor to total mortality
The findings of the study suggest that plasma levels of miR-let7a are strongly associated with an increased risk of the
diagnosis of Chronic Pulmonary Thromboembolic Hypertension during follow-up.
Dysregulated circulating miRNAs in n=177 patients
Analyzed miRNA patterns in plasma samples using array PCR-based profiling Increased miR-let7a above the optimal
cut-off, a risk of 12.8% higher at
diagnosis was considered

Plasma Levels
Chonic Trhromboembolic
pulmonary hypertension

miR-let7a ad miR-720
miR-29a

Elevated miR-29a and miR-720 3.4% of patients with Acute

levels Pulmonary Embolism
BIBLIOGRAPHY
Chirshev, E., Ioffe, Y. J., Oberg, K. C., & Unternaehrer, J. J. (28 de Agosto de 2019). Let-7 as biomarker, prognostic indicator, and therapy for
precision medicine in cancer. Obtenido de https://clintransmed.springeropen.com/articles/10.1186/s40169-019-0240-y

Graves, A., Sandhu, S., & Kingsley, K. (23 de Septiembre de 2020). Expression of miR-720 is correlated with DNMT3 in Oral squamous cell
carcinomas. Obtenido de https://exrna.biomedcentral.com/articles/10.1186/s41544-020-00055-0

Huang, Y.-H., Yang, Y.-L., & Wang, F.-S. (27 de Junio de 2018). The Role of miR-29a in the Regulation, Function, and Signaling of Liver
Fibrosis. Obtenido de https://www.mdpi.com/1422-0067/19/7/1889

Nelson, D. L., & Cox, M. M. (2018). Lehninger PRINCIPIOS de BIOQUÍMICA (Séptima Edición). Barcelona: Omega.

National Cancer Institute. (s.f.). Embolia Pulmonar. Obtenido de https://www.cancer.gov/espanol/publicaciones/diccionarios/diccionario-

cancer/def/embolia-pulmonar

Salisbury, J. P., Curbelo, P., Arcaus, M., & Cáneva, J. (27 de Junio de 2011). Hipertensión pulmonar tromboembólica crónica. Obtenido de
http://www.scielo.edu.uy/scielo.php?script=sci_arttext&pid=S1688-03902011000300007