January 2019, Volume 37, Number 1, pp.

1–116 Editor
Matthew Pavlovich

Scientific Life Trends Publisher

Paige Shaklee
1 AI Paradigms for Teaching Wilson Wen Bin Goh
Biotechnology and Chun Chau Sze Journal Manager
Rolf van der Sanden
Science & Society Journal Administrator
5 Regulatory Convergence for Biologics Jared R. Auclair Patrick Scheffmann

through Capacity Building and Training Advisory Editorial Board

Anthony Atala
9 The Increasingly Human and Profitable António L. Grilo and A. Mantalaris Carlijn Bouten
Monoclonal Antibody Market Joaquim Cabral
Alan Colman
Forum Don Cowan
Cees Dekker
16 Teaching Shrimps Self-Defense Parisa Norouzitallab, Martin Fussenegger
to Fight Infections Kartik Baruah, Daisy Vanrompay, J. Keith Joung
Douglas Kell
and Peter Bossier Robert Langer
Opinions Jiayang Li
Thomas Macek
20 Constructing RNA Viruses for Long- Juozas Baltusnikas, Bo Mattiasson
Term Transcriptional Gene Silencing Saulius Satkauskas, Anton Middelberg
and Kenneth Lundstrom Jean Peccoud
Jim Philp
Sergio Riva
29 Harnessing Underground Metabolism Jonathan Rosenberg Gregory Stephanopoulos
for Pathway Development and Fabian M. Commichau Anthony Turner
Tomasz Twardowski
Mathias Uhlén
Reviews Mark van Loosdrecht
Urs Von Stockar
38 RNAi/CRISPR Screens: from a Pool Anne Schuster, Hélène Erasimus,
Jim Wells
to a Valid Hit Sabrina Fritah, Petr V. Nazarov,
Eric van Dyck, Simone P. Niclou,
and Anna Golebiewska
56 Strategies for the Enrichment and Chonghua Ren, Kun Xu,
Selection of Genetically Modified Cells David Jay Segal,
and Zhiying Zhang

72 Single-Molecule Sequencing: Adam Ameur,

Towards Clinical Applications Wigard P. Kloosterman,
and Matthew S. Hestand

86 Nanowire Sensors in Cancer Marie-Agnès Doucey

and Sandro Carrara

100 Engineering Microbial Living Danielle B. Pedrolli,

Therapeutics: The Synthetic Biology Nathan V. Ribeiro,
Toolbox Patrick N. Squizato,
Series: iGEM Perspectives on Synthetic Victor N. de Jesus,
Biology Daniel A. Cozetto, and Team AQA
Unesp at iGEM 2017

On The Cover: State-of-the-art single-molecule sequencing platforms offer Editorial Inquiries

Volume 37 Number 1
January 2019
ISSN 0167-7799
Clinical Applications of
Single-Molecule Sequencing
exceptionally long reads with low biases and the ability to directly detect nucleotide base Trends in Biotechnology
modifications. These qualities enable sequencing of disease-causing long repetitive Cell Press
elements, complex gene loci, structural variations, and epigenetic marks that would be 50 Hampshire St. 5th Floor
difficult or impossible to analyze with previous high-throughput technologies. In pages Cambridge, MA 02139, USA
72–85 of this issue, Ameur and colleagues discuss how single-molecule sequencing is Tel: 617 386 2800
revolutionizing medical applications in pharmacogenomics, pathogenic microbiology, E-mail: tibtech@cell.com
constitutional disorder diagnoses, cancer, and more. Cover image by iStock/blackdovfx
and cover design by Joseph Yoo.