QUALITY CONTROL OF ANTISERA (A)

QUALITY REQUIREMENT FOR EACH NEW LOT

EVERY MONTH________________

NAME AVIDITY WORK

SPECIFICITY INTENSITY
AND WITH DONE
TYPE(FOR
ANTI D- NAME
DATE OF BATCH EXP. ROULEAUX PROZONE
MONO , OF THE
TESTING NO. DATE WITH POS WITH NEG 50 % RED (GRADE FORMATION PHENOMENON
POLY, KIT BY
BLEND) CONTROL CONTROL CELL +,++,+++,++++)
OF
REAGENT

TITRATION OF ANTISERA

TITRATION USING POOLED BLOOD GROUP" _____ " RED CELL ANTIGENS

DILUTION 1:1 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:256 1:512 TITER REMARKS
STRENGTH
WITH " "CELLS

CHECKED BY :__________________

DATE : ________________________

QUALITY
REQUIREMENTS SPECIFICITY TITRE
AVIDITY WITH 50 % INTENSITY(GRADE ROULEAUX PROZONE
RED CELL ( +,++,+++,++++) FORMATION PHENOMENON
WITH POS WITH NEG
CONTROL CONTROL
3+ in 3-4 secs 256

Macroscopic 4+ in 3-4 secs 256

Haemolysis or No Haemolysis or
Anti-A agglutination on slide in Absent Absent
agglutination agglutination
10 seconds 3+ in 5-6 secs 128

3+ in 5-10 secs 128-256

QUALITY CONTROL OF ANTISERA (B)

EVERY MONTH________________

NAME AVIDITY WORK

SPECIFICITY INTENSITY
AND WITH DONE
TYPE(FOR
ANTI D- NAME
DATE OF BATCH EXP. ROULEAUX PROZONE
MONO , OF THE
TESTING NO. DATE WITH POS WITH NEG 50 % RED (GRADE FORMATION PHENOMENON
POLY, KIT BY
BLEND) CONTROL CONTROL CELL +,++,+++,++++)
OF
REAGENT

TITRATION OF ANTISERA

TITRATION USING POOLED BLOOD GROUP" _____ " RED CELL ANTIGENS

DILUTION 1:1 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:256 1:512 TITER REMARKS
STRENGTH
WITH " "CELLS

CHECKED BY :__________________

DATE : ________________________

QUALITY
REQUIREMENTS SPECIFICITY TITRE
AVIDITY WITH 50 % INTENSITY(GRADE ROULEAUX PROZONE
RED CELL ( +,++,+++,++++) FORMATION PHENOMENON
WITH POS WITH NEG
CONTROL CONTROL
3+ in 3-4 secs 256

Macroscopic 4+ in 3-4 secs 256

Haemolysis or No Haemolysis or
Anti-B agglutination on slide in Absent Absent
agglutination agglutination
10 seconds 3+ in 5-6 secs 128

3+ in 5-10 secs 128-256

 QUALITY CONTROL OF ANTISERA (AB)

EVERY MONTH________________

NAME AVIDITY WORK

SPECIFICITY INTENSITY
AND WITH DONE
TYPE(FOR
ANTI D- NAME
DATE OF BATCH EXP. ROULEAUX PROZONE
MONO , OF THE
TESTING NO. DATE WITH POS WITH NEG 50 % RED (GRADE FORMATION PHENOMENON
POLY, KIT BY
BLEND) CONTROL CONTROL CELL +,++,+++,++++)
OF
REAGENT

TITRATION OF ANTISERA

TITRATION USING POOLED BLOOD GROUP" _____ " RED CELL ANTIGENS

DILUTION 1:1 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:256 1:512 TITER REMARKS
STRENGTH
WITH " "CELLS

CHECKED BY :__________________

DATE : ________________________

QUALITY
REQUIREMENTS SPECIFICITY TITRE
AVIDITY WITH 50 % INTENSITY(GRADE ROULEAUX PROZONE
RED CELL ( +,++,+++,++++) FORMATION PHENOMENON
WITH POS WITH NEG
CONTROL CONTROL
3+ in 3-4 secs 256

Macroscopic 4+ in 3-4 secs 256

Haemolysis or No Haemolysis or
Anti-AB agglutination on slide in Absent Absent
agglutination agglutination
10 seconds 3+ in 5-6 secs 128

3+ in 5-10 secs 128-256

QUALITY CONTROL OF ANTISERA [ D(Ig M) ]

EVERY MONTH________________

NAME AVIDITY WORK

SPECIFICITY INTENSITY
AND WITH DONE
TYPE(FOR
ANTI D- NAME
DATE OF BATCH EXP. ROULEAUX PROZONE
MONO , OF THE
TESTING NO. DATE WITH POS WITH NEG 50 % RED (GRADE FORMATION PHENOMENON
POLY, KIT BY
BLEND) CONTROL CONTROL CELL +,++,+++,++++)
OF
REAGENT

TITRATION OF ANTISERA

TITRATION USING POOLED BLOOD GROUP" _____ " RED CELL ANTIGENS

DILUTION 1:1 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:256 1:512 TITER REMARKS
STRENGTH
WITH " "CELLS

CHECKED BY :__________________

DATE : ________________________

QUALITY
REQUIREMENTS SPECIFICITY TITRE
AVIDITY WITH 50 % INTENSITY(GRADE ROULEAUX PROZONE
RED CELL ( +,++,+++,++++) FORMATION PHENOMENON
WITH POS WITH NEG
CONTROL CONTROL
3+ in 3-4 secs 256

Macroscopic 4+ in 3-4 secs 256

Haemolysis or No Haemolysis or
Anti-D agglutination on slide in Absent Absent
agglutination agglutination
10 seconds 3+ in 5-6 secs 128

3+ in 5-10 secs 128-256

 QUALITY CONTROL OF ANTISERA [ D(BLEND) ]

EVERY MONTH________________

NAME AVIDITY WORK

SPECIFICITY INTENSITY
AND WITH DONE
TYPE(FOR
ANTI D- NAME
DATE OF BATCH EXP. ROULEAUX PROZONE
MONO , OF THE
TESTING NO. DATE WITH POS WITH NEG 50 % RED (GRADE FORMATION PHENOMENON
POLY, KIT BY
BLEND) CONTROL CONTROL CELL +,++,+++,++++)
OF
REAGENT

TITRATION OF ANTISERA

TITRATION USING POOLED BLOOD GROUP" _____ " RED CELL ANTIGENS

DILUTION 1:1 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:256 1:512 TITER REMARKS
STRENGTH
WITH " "CELLS

CHECKED BY :__________________

DATE : ________________________

QUALITY
REQUIREMENTS SPECIFICITY TITRE
AVIDITY WITH 50 % INTENSITY(GRADE ROULEAUX PROZONE
RED CELL ( +,++,+++,++++) FORMATION PHENOMENON
WITH POS WITH NEG
CONTROL CONTROL
3+ in 3-4 secs 256

Macroscopic 4+ in 3-4 secs 256

Haemolysis or No Haemolysis or
Anti-D agglutination on slide in Absent Absent
agglutination agglutination
10 seconds 3+ in 5-6 secs 128

3+ in 5-10 secs 128-256

QUALITY CONTROL OF BOVINE SERUM ALBUMIN

QUALITY PARAMETERS TO BE CHECKED FOR EACH NEW LOT
EVERY MONTH________________

REACTION WITH
NAME OF THE DATE OF EXPIRY PROZONE WORK DONE
UNSENSITIZED RED POTENCY ROULEAUX FORMATION
KIT MANUFACTURE DATE PHENOMENON BY
CELLS

IgG anti-D
should give a
QUALITY REQUIREMENTS No agglutination titer of 32-64 None None
with O Rh (D)
red cells.

POTENCY : Reaction of IgG anti D with D(Rho) positive red cells

Dilution 1:1 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:256 1:512 TITER

Strength

CHECKED BY :__________________

DATE : ________________________
 QUALITY CONTROL OF ANTI HUMAN GLOBULIN
QUALITY PARAMETERS TO BE CHECKED FOR EACH NEW LOT
EVERY MONTH________________

DAT WITH DAT WITH

DATE OF EXPIRY PROZONE
NAME OF THE KIT SENSITISED UNSENSITIZED POTENCY ROULEAUX FORMATION WORK DONE BY
MANUFACTURE DATE PHENOMENON
CELLS CELLS

QUALITY No haemolysis or
Agglutination 1:64 None None
REQUIREMENTS agglutination

POTENCY : Reaction of IgG anti D with D(Rho) positive red cells

Dilution 1:1 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:256 1:512 TITER

Strength

CHECKED BY :__________________

DATE : ________________________