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Mu Estimation
Mu Estimation
Mu Estimation
Test Method :
Microorganism used for spiking :
Date Tested :
% Recovery of log
value
No. of CFU-Spiked Attachment CFU-Spike Attachment
Log10 value (A) Log10 value (B) (Log10 value
sample (A) Result Sample (B) Result (B)/Log10 value
(A) x 100
1
2
3
4
5
6
7
8
9
10
SUM % 0.0000
Mean % #DIV/0!
Therefore, reproducibility standard deviation is, SD % #DIV/0!
SDx2 % #DIV/0!
Mean% recovery of the log10 value = 99.7310% ; SD of % recovery = 2.0642%
Apply coverage factor (k=2 for 95% coverage)
Expanded MU = 2 x standard deviation (%)
2 x 2.0642% = 4.1283 = 0.04128
Test Item result = 440 cfu/g or cfu/ml, convert 440 in log10 = 2.6434
Expanded Uncertainty for log counts = 2.6434 x 0.04128 = 0.1091
The Interval in log10 count is :
Lower bound 2.6434 – 0.1091= 2.5343 and
Upper bound 2.6434 + 0.1091 = 2.7525
Calculatation of lower and upper bound
lower bound 102.5343= 342 and
Upper bound 102.7525 = 566
No. of
Sample Decription Samples Type
samples
1 Jamu Herba Powder
2 Jamu Herba Powder
3 Jamu Herba Powder
4 Jamu Herba Powder
5 Kapsul Herba Orang
Hard Gel capsules
Kampung
6 Kapsul Herba Orang
Hard Gel capsules
Kampung
7 Flaxseed Oil Softgel
8 Flaxseed Oil Softgel
9 Flaxseed Oil Softgel
10 Flaxseed Oil Softgel
11 Stomach Hurix Tablet
12 Stomach Hurix Tablet
13 Stomach Hurix Tablet
14 Stomach Hurix Tablet
15 Pil Hong Sah Pill
16 Pil Hong Sah Pill
17 Air Gamat Water/Syrup
18 Air Gamat Water/Syrup
19 Air Gamat Water/Syrup
20 Air Gamat Water/Syrup