ence between two locations on a target should be detected and displayed as a 1.0�C (1.8�F) temperature difference by a properly calibrated thermal imager; i.e., the Thermal Gain should be 1.0. This 1:1 relationship should remain constant whether the measurement is near room tem- perature (Troom, ~20�C/68�F), body temperature (Tbody, ~37�C/98.6�F), or at any temperature in between. If the thermal gain is in error, a 1.0�C (1.8�F) temperature dif- ference on a target may be displayed by the imager as only 0.2�C (0.36�F) or some other wrong value, making the quantitative measurements erroneous, yet the qualitative image may still appear completely normal by adjusting the color span.2 As the quantitative interpretation of human thermograms relies heavily on differential temperature measurements, assuring a Thermal Gain between 0.95 and 1.05 (preferably 1.0) across the physiologic temperature range is of paramount importance. 2. Temperature Offset. This refers to the temperature dif- ference between the target surface temperature detected by the infrared imager and the actual temperature of that surface. Temperature offset and thermal gain are related: if the imager�s offset value is stable across the physiologic temperature range, the thermal gain will be 1.0 over those temperatures. Stable offset values up to �2�C (3.6�F) are typical for professional microbolometer imagers. 3. Temperature Flatness. �Flatness� measures the consis- tency of a constant temperature object as sensed at different regions of the image, usually measured at the center and four corners of the image at Troom. The imager should be fully warmed up before this test so that the imaging chip has reached a stable temperature. The flatness of the image at Tbody can also be measured by using a Blackbody Calibrator.