DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY

Mid La Union Campus

City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

OBSERVATION OF DROSOPHILA
GENETICS
1st Semester
S.Y 2023-2024

Submitted to:
Ma’am Ireshlyn Potestades Mendoza

Submitted by:
Angelika G. Pimentel
&
Jasmine Habon

Bachelor of Secondary Education Major in Science

3E
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

Set up the Housing of Drosophila

Begin by crafting a culture medium using tomatoes as the primary ingredient. This can
be achieved by the chopped tomatoes, which serves as a nutritious substrate for the
flies. Afterwards transfer the chopped tomatoes into clean jar, providing ample space
for the Drosophila to navigate. This jar serves as their living environment.
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

After the set-up

On the first day, the drosophila (fruit flies) enter the housing setup which is the jar
containing chopped tomatoes that entices them to enter.
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

Exploration and acclimation

We then observe that the fruit flies tend to explore their surroundings, flying and
walking around to familiarize themselves with the available space. They also might
engage in feeding activities, exploring the provided food source (chopped tomatoes).
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

Sex Identification

We determine which of them is a male and female drosophila for me to be able to

separate 1 female and 1 male drosophilia through the process of cooling method to
immobilized them. To distinguish between male and female fruit flies can be
challenging without specialized equipment. But according to research, female fruit
flies typically have a larger and more rounded abdomen and has red eyes, while males
have slimmer, more pointed abnomen and has white eyes. It also has darker
pigmentation on their posterior.
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

Separation and Ongoing mating

After multiple trials we then identify the male and female fruit flies, and these
selected flies were placed on Tupperware.
During mating: We observe that the male positions himself near the female,
frequently tapping her with his forelegs. The male may extend also vibrate his wings,
showcasing to the female is also observed.
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

Egg-laying Behaviors

During this process, we observe that the female drosophila carefully selecting a
suitable location for laying her eggs (usually white and very small, often challenging
to see without magnification). And according to what we’ve read, female fruit flies
typically prefer laying eggs on decaying or fermenting fruits, vegetables or other
organic matter.
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

Larvae development and pupation phase

(Transition from larvae to pupae)

Larvae exhibit explanatory movements, climbing surfaces and actively seeking a

suitable location for pupation. The fruit fly larvae initiate the preparation for
pupation by creating a cocoon. Cocoon is formed around the pupa. It encases the
developing pupa and is a distinctive feature in the transition from larvae to the pupal
stage.
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

And then, the emergence of adult flies from pupae and continuation of life cycle
stages, potential for increased population.
Punnett square of Drosophila

Father Mother

♂️ ♀️

P generation: Xw+Y × Xw+Xw+

Gametes

♂️: Xw+Y

♀️: Xw+Xw+

Punnet square

♂️

Xw+ Y

Xw+ Xw+Xw+ Xw+Y

Xw+ Xw+Xw+ Xw+Y

Phenotype:
 DON MARIANO MARCOS MEMORIAL STATE UUNIVERSITY
Mid La Union Campus
City of San Fernando, La Union College of Education
BACHELOR OF SECONDARY EDUCATION
MAJOR IN SCIENCE

2 homozygous Xw+Xw+: 2 hemizygous Xw+Y

Genotype:
2 red eye female drosophila
2 white eye male drosophila

Reference:
https://byjus.com/neet/difference-between-male-and-female-drosophila-
melanogaster/#:~:text=The%20differentiating%20factor%20between%20the,abdomen
%20with%20comparatively%20more%20stripes.