Glycerol-Free Taq HS, 50U/µL

HIGH CONCENTRATION, LYOPHILIZATION‑COMPATIBLE PCR POLYMERASE

FOR PREPARATION OF DRIED AMPLIFICATION MIXES

Glycerol‑Free Taq Hot‑Start has been optimized to deliver an excellent, stable performance
without glycerol (Fig. 1), meaning it is also compatible with lyophilization, as a stand‑alone
enzyme or when combined with additional assay components in a reaction mix.

Glycerol‑Free Taq Hot‑Start is supplied as a separate 50 U/µL, high‑performance glycerol‑free Taq, glycerol‑free Taq antibody and
glycerol‑free enzyme dilution buffer. Separating these components allows greater flexibility in assay development. Formulating the
components at high concentrations means Glycerol‑Free Taq Hot‑Start is especially suited for high throughput applications.

110

Glycerol‑free: convenient for lyophilization 100

High concentration: ideal for assay development 90

% Activity

80

Sensitive: retains the same reproducible performance as 70

with conventional formats
60

Convenient: confers improved stability at ambient 50

0 10 15
temperature F/T Cycles

Flexible: separate Taq, antibody and enzyme dilution buffer,
for greater flexibility
Fig. 1. Stability
Glycerol is a cryoprotectant and is normally part of the storage buffer, where it serves to
protect Taq polymerase during freezing conditions. To test the resistance of Glycerol‑Free Taq
Hot‑Start to freezing and thawing, a freeze‑thaw test was performed (10 and 15 cycles of freeze/
thawing) and compared to fresh product (0 cycles of freeze/thawing). The results illustrate that
Glycerol‑Free Taq Hot‑Start offers the same protection to freeze/thawing as a polymerase that
contains glycerol.

meridianbioscience.com/lifescience
Glycerol‑Free Taq Hot‑Start delivers the same accurate, excellent
results under fast qPCR conditions from the reconstituted mix as
it does from the wet mix (Fig. 2).

Glycerol‑Free Taq Hot‑Start gives the same robust, high‑yield

amplification following lyophilization, as MyTaq HS DNA
Polymerase (Fig. 3).

An additional benefit of lyophilization is that larger volumes of

Fig. 2. Efficient and reproducible qPCR assays
sample can be added, thereby increasing the potential sensitivity Glycerol‑Free Taq Hot‑Start was used to set up two sets of qPCR assays, one assay was
of the assay even further. then lyophilized (blue) and the other left as a wet mix (black). A 10‑fold serial dilution
of template DNA was used as a template for the qPCR reactions. The results illustrate
equally efficient and sensitive amplification was achieved with both wet and lyophilized
Glycerol‑Free Taq Hot‑Start.

APPLICATIONS
MyTaq HS
Glycerol‑Free Taq Hot‑Start has been tested with a range of 35.00
Glycerol-Free Taq HS 50U/µL
template and primers and is perfectly suited to the following 30.00

applications: 25.00

20.00
Ct

• Diagnostic test development 15.00

• Pathogen detection 10.00

• Drug therapy efficacy 5.00

• DNA target detection 0.00

8.5 0.85 0.085 0.0085 0.00085 0.000085
• Gene expression analysis Template Concentration (pg/mL)

Fig. 3. High sensitivity

Glycerol‑Free Taq Hot‑Start and MyTaq DNA Polymerase were used to amplify a fragment
of DNA from varying amounts of template DNA in triplicate assays. Glycerol‑Free Taq
Hot‑Start offers the same sensitivity, specificity and yields, comparable to those of the
standard formulation of MyTaq DNA Polymerase.

Product Size Cat. #

1,000 Units BIO‑11070
Glycerol-Free Taq HS, 50U/µL
25,000 Units BIO‑11070.01

Ordering information:
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