Report of NFS

Specimen Name: NFS Run Time: 15/03/2024 12:33

Cytometer: NovoCyte Advanteon 711220820250 Software: NovoExpress 1.6.2

JC1 JC1 JC1 / Hemo

Aggregates
3,23% Cells
Dead 67,79% Hemo'
23,87% Eosino
99,52% Eosino'
16,24% 16,18%
Hyalino'
Hyalino 60,12%
Baso Baso'
34,44%
16,39% 22,77%
Hemo
SPZ 67,77%
2,18%

Sample Statistics of JC1

Gate Count % Parent X Y Median X Median Y

All 66 717

Hyalino 22 977 34,44 % FSC-H SSC-H 1 250 747 1 385 796

Baso 10 935 16,39 % FSC-H SSC-H 747 889 549 027

Eosino 10 838 16,24 % FSC-H SSC-H 1 702 224 2 649 199

Aggregates 2 154 3,23 % FSC-H SSC-H 2 670 515 4 365 849

Cells 45 229 67,79 % FSC-H SSC-H 1 224 611 1 401 715

Dead 15 923 23,87 % FSC-H SSC-H 1 157 877 1 648 202

SPZ 1 452 2,18 % FSC-H SSC-H 302 016 114 470

Hemo 45 211 67,77 % FSC-H SSC-H 1 213 559 1 368 655

Hemo' 44 993 99,52 % FSC-H SSC-H 1 213 031 1 365 252

Baso' 10 296 22,77 % FSC-H SSC-H 747 327 550 184

Hyalino' 27 183 60,12 % FSC-H SSC-H 1 254 708 1 436 683

Eosino' 7 316 16,18 % FSC-H SSC-H 1 791 563 2 923 042

LS2 LS2 LS2 / Hemo

Aggregates
16,85% Cells
Dead 43,82% Hemo'
11,80% Eosino
82,14% Eosino'
8,99% 46,43%
Hyalino'
Hyalino 17,86%
Baso Baso'
2,25%
1,69% 14,29%
Hemo
SPZ 15,73%
1,12%

1/11
Sample Statistics of LS2

Gate Count % Parent X Y Median X Median Y

All 178

Hyalino 4 2,25 % FSC-H SSC-H 1 213 940 1 114 118

Baso 3 1,69 % FSC-H SSC-H 690 398 863 263

Eosino 16 8,99 % FSC-H SSC-H 1 980 439 4 851 580

Aggregates 30 16,85 % FSC-H SSC-H 5 151 028 5 320 079

Cells 78 43,82 % FSC-H SSC-H 2 161 825 4 556 971

Dead 21 11,80 % FSC-H SSC-H 557 530 1 175 595

SPZ 2 1,12 % FSC-H SSC-H 286 798 157 696

Hemo 28 15,73 % FSC-H SSC-H 1 717 687 3 740 088

Hemo' 23 82,14 % FSC-H SSC-H 1 612 431 3 792 070

Baso' 4 14,29 % FSC-H SSC-H 667 664 766 890

Hyalino' 5 17,86 % FSC-H SSC-H 1 300 816 1 322 229

Eosino' 13 46,43 % FSC-H SSC-H 1 981 148 4 840 629

HM2 HM2 HM2 / Hemo

Aggregates
27,66% Cells
Dead 63,83% Hemo'
6,38% Eosino
84,21% Eosino'
9,57% 36,84%
Hyalino'
Hyalino 15,79%
Baso Baso'
4,26%
4,26% 21,05%
Hemo
SPZ 20,21%
4,26%

Sample Statistics of HM2

Gate Count % Parent X Y Median X Median Y

All 94

Hyalino 4 4,26 % FSC-H SSC-H 1 732 202 1 519 558

Baso 4 4,26 % FSC-H SSC-H 532 820 412 944

Eosino 9 9,57 % FSC-H SSC-H 2 642 369 5 332 261

Aggregates 26 27,66 % FSC-H SSC-H 5 536 435 8 926 146

Cells 60 63,83 % FSC-H SSC-H 2 780 914 5 317 684

Dead 6 6,38 % FSC-H SSC-H 507 132 1 172 089

SPZ 4 4,26 % FSC-H SSC-H 340 373 133 895

Hemo 19 20,21 % FSC-H SSC-H 2 284 714 2 976 726

Hemo' 16 84,21 % FSC-H SSC-H 1 732 202 2 970 545

Baso' 4 21,05 % FSC-H SSC-H 532 820 412 944

Hyalino' 3 15,79 % FSC-H SSC-H 1 703 917 1 353 595

Eosino' 7 36,84 % FSC-H SSC-H 2 430 848 5 332 261

2/11
 LV2 LV2 LV2 / Hemo
Aggregates
1,31% Cells
Dead 55,99% Hemo'
16,05% 99,66% Eosino'
Eosino
4,59% 4,66%
Hyalino'
Hyalino 57,34%
Baso Baso'
28,26%
21,75% 37,67%
Hemo
SPZ 55,51%
4,03%

Sample Statistics of LV2

Gate Count % Parent X Y Median X Median Y

All 28 787

Hyalino 8 134 28,26 % FSC-H SSC-H 1 212 683 1 253 314

Baso 6 262 21,75 % FSC-H SSC-H 759 989 622 560

Eosino 1 321 4,59 % FSC-H SSC-H 1 939 439 2 545 004

Aggregates 377 1,31 % FSC-H SSC-H 3 022 197 4 773 788

Cells 16 119 55,99 % FSC-H SSC-H 1 029 548 1 020 929

Dead 4 620 16,05 % FSC-H SSC-H 912 694 1 165 545

SPZ 1 161 4,03 % FSC-H SSC-H 305 163 92 410

Hemo 15 980 55,51 % FSC-H SSC-H 1 025 272 1 010 928

Hemo' 15 925 99,66 % FSC-H SSC-H 1 024 785 1 010 056

Baso' 6 019 37,67 % FSC-H SSC-H 756 986 618 403

Hyalino' 9 163 57,34 % FSC-H SSC-H 1 202 705 1 246 075

Eosino' 744 4,66 % FSC-H SSC-H 2 089 938 2 878 706

IV2 IV2 IV2 / Hemo

Aggregates
1,46% Cells
Dead 32,84% Hemo'
14,16% 99,40% Eosino'
Eosino
3,59% 6,76%
Hyalino'
Hyalino 66,97%
Baso Baso'
18,81%
8,92% 25,33%
Hemo
SPZ 31,86%
4,77%

3/11
Sample Statistics of IV2

Gate Count % Parent X Y Median X Median Y

All 35 991

Hyalino 6 770 18,81 % FSC-H SSC-H 1 186 804 1 260 084

Baso 3 212 8,92 % FSC-H SSC-H 718 328 627 544

Eosino 1 293 3,59 % FSC-H SSC-H 1 938 108 2 599 778

Aggregates 524 1,46 % FSC-H SSC-H 3 247 923 5 337 943

Cells 11 820 32,84 % FSC-H SSC-H 1 096 100 1 174 614

Dead 5 096 14,16 % FSC-H SSC-H 854 978 1 133 706

SPZ 1 717 4,77 % FSC-H SSC-H 302 670 101 175

Hemo 11 468 31,86 % FSC-H SSC-H 1 088 442 1 142 173

Hemo' 11 399 99,40 % FSC-H SSC-H 1 088 256 1 139 893

Baso' 2 905 25,33 % FSC-H SSC-H 720 361 639 321

Hyalino' 7 680 66,97 % FSC-H SSC-H 1 178 893 1 261 776

Eosino' 775 6,76 % FSC-H SSC-H 2 044 802 2 940 434

ML2A ML2A ML2A / Hemo

Aggregates
0,42% Cells
Dead 64,87% Hemo'
11,51% 99,69% Eosino'
Eosino
3,29% 2,18%
Hyalino'
Hyalino 66,56%
Baso Baso'
39,74%
21,55% 30,81%
Hemo
SPZ 65,66%
2,20%

Sample Statistics of ML2A

Gate Count % Parent X Y Median X Median Y

All 7 332

Hyalino 2 914 39,74 % FSC-H SSC-H 1 277 605 1 103 450

Baso 1 580 21,55 % FSC-H SSC-H 750 543 567 439

Eosino 241 3,29 % FSC-H SSC-H 2 129 082 2 438 091

Aggregates 31 0,42 % FSC-H SSC-H 2 910 092 3 604 140

Cells 4 756 64,87 % FSC-H SSC-H 1 122 841 976 963

Dead 844 11,51 % FSC-H SSC-H 844 551 1 069 654

SPZ 161 2,20 % FSC-H SSC-H 300 877 117 316

Hemo 4 814 65,66 % FSC-H SSC-H 1 116 612 966 539

Hemo' 4 799 99,69 % FSC-H SSC-H 1 116 220 966 540

Baso' 1 483 30,81 % FSC-H SSC-H 745 757 563 039

Hyalino' 3 204 66,56 % FSC-H SSC-H 1 265 304 1 091 318

Eosino' 105 2,18 % FSC-H SSC-H 2 368 256 2 798 553

4/11
 IF IF IF / Hemo
Aggregates
0,44% Cells
Dead 71,38% Hemo'
18,59% 99,82% Eosino'
Eosino
2,67% 2,22%
Hyalino'
Hyalino 49,30%
Baso Baso'
31,86%
36,36% 48,53%
Hemo
SPZ 72,50%
1,77%

Sample Statistics of IF

Gate Count % Parent X Y Median X Median Y

All 11 486

Hyalino 3 660 31,86 % FSC-H SSC-H 1 162 804 1 161 105

Baso 4 176 36,36 % FSC-H SSC-H 737 849 546 406

Eosino 307 2,67 % FSC-H SSC-H 1 727 113 2 528 741

Aggregates 51 0,44 % FSC-H SSC-H 2 722 209 4 001 191

Cells 8 199 71,38 % FSC-H SSC-H 936 684 873 309

Dead 2 135 18,59 % FSC-H SSC-H 921 035 1 163 839

SPZ 203 1,77 % FSC-H SSC-H 298 729 100 242

Hemo 8 327 72,50 % FSC-H SSC-H 928 628 860 762

Hemo' 8 312 99,82 % FSC-H SSC-H 928 456 860 722

Baso' 4 041 48,53 % FSC-H SSC-H 736 937 544 935

Hyalino' 4 105 49,30 % FSC-H SSC-H 1 142 807 1 141 291

Eosino' 185 2,22 % FSC-H SSC-H 1 854 560 2 771 428

CV2 CV2 CV2 / Hemo

Aggregates
0,17% Cells
Dead 13,50% Hemo'
16,34% Eosino
99,57%Eosino'
2,17% 12,12%
Hyalino'
Hyalino 58,56%
Baso Baso'
5,78%
3,85% 28,42%
Hemo
SPZ 12,74%
1,07%

5/11
Sample Statistics of CV2

Gate Count % Parent X Y Median X Median Y

All 66 047

Hyalino 3 818 5,78 % FSC-H SSC-H 1 116 004 1 350 533

Baso 2 544 3,85 % FSC-H SSC-H 681 391 659 952

Eosino 1 434 2,17 % FSC-H SSC-H 1 538 269 2 614 736

Aggregates 115 0,17 % FSC-H SSC-H 2 336 614 3 870 707

Cells 8 919 13,50 % FSC-H SSC-H 993 296 1 330 647

Dead 10 792 16,34 % FSC-H SSC-H 496 207 958 923

SPZ 707 1,07 % FSC-H SSC-H 300 750 105 717

Hemo 8 416 12,74 % FSC-H SSC-H 1 012 637 1 251 032

Hemo' 8 380 99,57 % FSC-H SSC-H 1 013 764 1 249 325

Baso' 2 392 28,42 % FSC-H SSC-H 681 607 671 840

Hyalino' 4 928 58,56 % FSC-H SSC-H 1 091 070 1 393 446

Eosino' 1 020 12,12 % FSC-H SSC-H 1 593 855 2 886 216

PG2 PG2 PG2 / Hemo

Aggregates
0,37% Cells
Dead 75,98% Hemo'
33,33% 99,91% Eosino'
Eosino
5,40% 3,71%
Hyalino'
Hyalino 66,31%
Baso Baso'
44,25%
24,25% 30,18%
Hemo
SPZ 76,47%
1,33%

Sample Statistics of PG2

Gate Count % Parent X Y Median X Median Y

All 14 028

Hyalino 6 208 44,25 % FSC-H SSC-H 1 120 386 1 219 542

Baso 3 402 24,25 % FSC-H SSC-H 757 999 714 569

Eosino 758 5,40 % FSC-H SSC-H 1 736 761 2 446 252

Aggregates 52 0,37 % FSC-H SSC-H 2 481 299 3 934 082

Cells 10 658 75,98 % FSC-H SSC-H 1 001 485 1 087 938

Dead 4 676 33,33 % FSC-H SSC-H 923 494 1 181 371

SPZ 187 1,33 % FSC-H SSC-H 309 280 123 224

Hemo 10 727 76,47 % FSC-H SSC-H 998 483 1 078 548

Hemo' 10 717 99,91 % FSC-H SSC-H 998 459 1 078 301

Baso' 3 237 30,18 % FSC-H SSC-H 754 442 708 525

Hyalino' 7 113 66,31 % FSC-H SSC-H 1 104 107 1 210 813

Eosino' 398 3,71 % FSC-H SSC-H 1 857 040 2 767 450

6/11
 ML2 ML2 ML2 / Hemo
Aggregates
0,75% Cells
Dead 22,95% Hemo'
8,30% 99,27% Eosino'
Eosino
2,72% 8,14%
Hyalino'
Hyalino 50,35%
Baso Baso'
9,86%
9,75% 40,07%
Hemo
SPZ 22,47%
7,18%

Sample Statistics of ML2

Gate Count % Parent X Y Median X Median Y

All 34 246

Hyalino 3 376 9,86 % FSC-H SSC-H 1 197 886 1 197 640

Baso 3 339 9,75 % FSC-H SSC-H 697 841 479 671

Eosino 930 2,72 % FSC-H SSC-H 1 651 806 2 670 263

Aggregates 258 0,75 % FSC-H SSC-H 3 010 690 5 041 652

Cells 7 859 22,95 % FSC-H SSC-H 1 013 768 991 685

Dead 2 842 8,30 % FSC-H SSC-H 892 830 1 169 711

SPZ 2 460 7,18 % FSC-H SSC-H 309 402 86 289

Hemo 7 694 22,47 % FSC-H SSC-H 1 010 108 968 370

Hemo' 7 638 99,27 % FSC-H SSC-H 1 012 062 969 505

Baso' 3 083 40,07 % FSC-H SSC-H 703 378 489 454

Hyalino' 3 874 50,35 % FSC-H SSC-H 1 190 366 1 203 929

Eosino' 626 8,14 % FSC-H SSC-H 1 706 833 2 897 228

MV2 MV2 MV2 / Hemo

Aggregates
0,07% Cells
Dead 2,34% Hemo'
4,45% Eosino
98,32% Eosino'
0,33% 15,58%
Hyalino'
Hyalino 26,54%
Baso Baso'
0,39%
1,26% 53,92%
Hemo
SPZ 2,10%
0,20%

7/11
Sample Statistics of MV2

Gate Count % Parent X Y Median X Median Y

All 659 738

Hyalino 2 540 0,39 % FSC-H SSC-H 1 131 241 1 230 901

Baso 8 316 1,26 % FSC-H SSC-H 542 129 498 584

Eosino 2 175 0,33 % FSC-H SSC-H 1 555 524 3 265 704

Aggregates 436 0,07 % FSC-H SSC-H 2 730 319 4 893 794

Cells 15 412 2,34 % FSC-H SSC-H 829 620 1 508 628

Dead 29 358 4,45 % FSC-H SSC-H 365 594 735 864

SPZ 1 339 0,20 % FSC-H SSC-H 296 964 139 779

Hemo 13 834 2,10 % FSC-H SSC-H 788 440 828 875

Hemo' 13 601 98,32 % FSC-H SSC-H 778 596 818 478

Baso' 7 459 53,92 % FSC-H SSC-H 559 572 520 584

Hyalino' 3 671 26,54 % FSC-H SSC-H 1 063 444 1 314 399

Eosino' 2 155 15,58 % FSC-H SSC-H 1 531 785 3 322 474

TG2 TG2 TG2 / Hemo

Aggregates
0,63% Cells
Dead 25,03% Hemo'
13,73% 99,42% Eosino'
Eosino
2,13% 5,06%
Hyalino'
Hyalino 55,46%
Baso Baso'
11,85%
9,94% 38,59%
Hemo
SPZ 24,30%
1,41%

Sample Statistics of TG2

Gate Count % Parent X Y Median X Median Y

All 52 051

Hyalino 6 169 11,85 % FSC-H SSC-H 1 230 818 1 286 460

Baso 5 173 9,94 % FSC-H SSC-H 708 513 530 281

Eosino 1 111 2,13 % FSC-H SSC-H 1 857 824 2 505 559

Aggregates 327 0,63 % FSC-H SSC-H 3 390 416 6 492 546

Cells 13 027 25,03 % FSC-H SSC-H 1 037 111 1 094 857

Dead 7 148 13,73 % FSC-H SSC-H 550 249 943 260

SPZ 736 1,41 % FSC-H SSC-H 295 701 120 406

Hemo 12 650 24,30 % FSC-H SSC-H 1 030 516 1 016 158

Hemo' 12 576 99,42 % FSC-H SSC-H 1 030 914 1 014 059

Baso' 4 882 38,59 % FSC-H SSC-H 711 463 537 259

Hyalino' 7 016 55,46 % FSC-H SSC-H 1 220 256 1 287 087

Eosino' 640 5,06 % FSC-H SSC-H 1 969 934 2 878 238

8/11
 PL2 PL2 PL2 / Hemo
Aggregates
13,53% Cells
Dead 31,76% Hemo'
2,94% Eosino
93,33% Eosino'
2,94% 33,33%
Hyalino'
Hyalino 26,67%
Baso Baso'
1,76%
2,35% 20,00%
Hemo
SPZ 8,82%
2,35%

Sample Statistics of PL2

Gate Count % Parent X Y Median X Median Y

All 170

Hyalino 3 1,76 % FSC-H SSC-H 1 603 344 1 339 831

Baso 4 2,35 % FSC-H SSC-H 753 631 358 753

Eosino 5 2,94 % FSC-H SSC-H 2 332 339 5 240 911

Aggregates 23 13,53 % FSC-H SSC-H 5 840 223 11 621 003

Cells 54 31,76 % FSC-H SSC-H 2 944 674 5 185 420

Dead 5 2,94 % FSC-H SSC-H 321 781 750 210

SPZ 4 2,35 % FSC-H SSC-H 312 171 65 641

Hemo 15 8,82 % FSC-H SSC-H 1 796 745 2 376 078

Hemo' 14 93,33 % FSC-H SSC-H 1 752 277 1 889 234

Baso' 3 20,00 % FSC-H SSC-H 606 441 365 791

Hyalino' 4 26,67 % FSC-H SSC-H 1 655 576 1 371 111

Eosino' 5 33,33 % FSC-H SSC-H 2 332 339 5 289 836

TV2 TV2 TV2 / Hemo

Aggregates
1,15% Cells
Dead 25,06% Hemo'
17,99% 99,39% Eosino'
Eosino
1,89% 5,38%
Hyalino'
Hyalino 48,40%
Baso Baso'
9,97%
11,25% 44,63%
Hemo
SPZ 23,64%
2,89%

9/11
Sample Statistics of TV2

Gate Count % Parent X Y Median X Median Y

All 21 614

Hyalino 2 154 9,97 % FSC-H SSC-H 1 188 204 1 247 273

Baso 2 432 11,25 % FSC-H SSC-H 658 852 548 627

Eosino 409 1,89 % FSC-H SSC-H 1 850 676 2 694 738

Aggregates 249 1,15 % FSC-H SSC-H 3 443 092 6 405 497

Cells 5 416 25,06 % FSC-H SSC-H 949 523 1 121 395

Dead 3 889 17,99 % FSC-H SSC-H 510 389 891 501

SPZ 624 2,89 % FSC-H SSC-H 298 722 118 015

Hemo 5 109 23,64 % FSC-H SSC-H 949 620 955 505

Hemo' 5 078 99,39 % FSC-H SSC-H 948 616 951 489

Baso' 2 280 44,63 % FSC-H SSC-H 664 824 558 551

Hyalino' 2 473 48,40 % FSC-H SSC-H 1 168 697 1 254 122

Eosino' 275 5,38 % FSC-H SSC-H 1 955 765 2 974 957

EL2 EL2 EL2 / Hemo

Aggregates
0,42% Cells
Dead 36,56% Hemo'
11,64% 99,66% Eosino'
Eosino
0,82% 1,50%
Hyalino'
Hyalino 14,46%
Baso Baso'
4,52%
34,80% 83,59%
Hemo
SPZ 40,15%
2,33%

Sample Statistics of EL2

Gate Count % Parent X Y Median X Median Y

All 8 801

Hyalino 398 4,52 % FSC-H SSC-H 1 094 384 1 117 687

Baso 3 063 34,80 % FSC-H SSC-H 644 606 476 628

Eosino 72 0,82 % FSC-H SSC-H 1 719 207 2 670 022

Aggregates 37 0,42 % FSC-H SSC-H 3 722 993 8 617 285

Cells 3 218 36,56 % FSC-H SSC-H 712 294 557 238

Dead 1 024 11,64 % FSC-H SSC-H 541 524 726 042

SPZ 205 2,33 % FSC-H SSC-H 299 408 122 032

Hemo 3 534 40,15 % FSC-H SSC-H 680 890 518 288

Hemo' 3 522 99,66 % FSC-H SSC-H 679 772 517 954

Baso' 2 954 83,59 % FSC-H SSC-H 647 245 479 760

Hyalino' 511 14,46 % FSC-H SSC-H 1 054 182 1 100 558

Eosino' 53 1,50 % FSC-H SSC-H 1 669 890 3 011 112

10/11
 CM2 CM2 CM2 / Hemo
Aggregates
0,00% Cells
Dead 0,00% Hemo'
23,53% Eosino
100,00% Eosino'
0,00% 0,00%
Hyalino'
Hyalino 0,00%
Baso Baso'
0,00%
11,76% 100,00%
Hemo
SPZ 5,88%
0,00%

Sample Statistics of CM2

Gate Count % Parent X Y Median X Median Y

All 17

Hyalino 0 0,00 % FSC-H SSC-H 0 0

Baso 2 11,76 % FSC-H SSC-H 430 423 510 760

Eosino 0 0,00 % FSC-H SSC-H 0 0

Aggregates 0 0,00 % FSC-H SSC-H 0 0

Cells 0 0,00 % FSC-H SSC-H 0 0

Dead 4 23,53 % FSC-H SSC-H 379 368 423 630

SPZ 0 0,00 % FSC-H SSC-H 0 0

Hemo 1 5,88 % FSC-H SSC-H 467 038 622 406

Hemo' 1 100,00 % FSC-H SSC-H 467 038 622 406

Baso' 1 100,00 % FSC-H SSC-H 467 038 622 406

Hyalino' 0 0,00 % FSC-H SSC-H 0 0

Eosino' 0 0,00 % FSC-H SSC-H 0 0

11/11