+2 Bio Zoo em 1 6

-: GOD GRACE :-
BIO-ZOOLOGY
REVISED EDITION (2023-24)
(Including the Govt.Question paper-2023)
( PART –I )
1. Reproduction in Organisms
2. Human Reproduction
3. Reproductive Health
4. Principles of Inheritance and Variation
5. Molecular Genetics
6. Evolution
Prepared by :-
P.SENGUTTUVAN. M.Sc.,M.Ed.,M.Phil.
P.G.T-Zoology –GHSS –Thoppur.
CELL : 9865449511
&
C.VALARMATHI. M.Sc.,M.Ed.,M.Phil.
P.G.T-Zoology.GHSS-Palayampudur
DHARMAPURI –DIST.
P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 1

1: REPRODUCTION OF ORGANISMS
BOOK BACK QUESTION
(Note :- Question no :1– 4 answers are available in your book )
5. Name an organism where cell division is itself a mode of reproduction.?
 Ameoba , Euglena, Paramecium.
6. Name the phenomenon where the female gamete directly develops into a new organism with an avian
example.
 Phenomenon - Parthenogenesis.
 In some organisms female gamete (ovum) is directly develop into a complete individual by physical or
chemical induction. e.g., Turkey, Chicken, Pigeon.
7. What is parthenogenesis? Give two examples from animals ? (May -2022)

 The process of development of an egg into a complete individual without fertilization is known as
parthenogenesis . Eg. Annelid and sea urchin eggs.
8. Which type of reproduction is effective -Asexual or sexual and why?

 Sexual reproduction is an effective method of reproduction than asexual method because Sexual
reproduction contributes to the evolution of the species by adding variation in a population.
 Variation occurs because of the fusion of male and female gametes (sexual reproduction) carrying
different sets of chromosomes.
9. The unicellular organisms which reproduce by binary fission are considered immortal. Justify.
 In Asexual reproduction single individual can able to producing offspring.
 The parent cells undergo to directly amitotic or mitotic division and produce young ones. So the
unicellular organisms are immortal.
10. Why is the offspring formed by asexual reproduction referred as a clone ?

 In Asexual reproduction single individual can able to producing offspring.
 These offspring are genetically and morphologically similar to one another and also similar to their parent.
 Clone is the term given to organisms that are similar in all aspects.
 Thus the offspring produced by asexual reproduction are called clones.
11. Give reasons for the following:-

a. Some organisms like honey bees are called parthenogenetic animals
Reason : Male Honey bee (Drones) is develop from unfertilized egg so they are called a
parthenogenetic animals.
b. A male honey bee has 16 chromosomes where as its female has 32 chromosomes.
Reason : 1. A male honey bee – Develop from unfertilized eggs.
2. Female honey bee - Develop from fertilized eggs.

12. Differentiate between the following :-
a) Binary fission in amoeba and multiple fission in Plasmodium :-
Sl.No Binary fission in Amoeba Multiple fission in Plasmodium
1. binary fission is seen in Amoeba like irregular In multiple fission the parent body divides into
shaped organisms many similar daughter cells simultaneously.
2. The nucleoli disintegrate and the nucleus divides First, the nucleus divides repeatedly without the
mitotically. The cell then constricts in the division of the cytoplasm, later the cytoplasm
middle, so the cytoplasm divides and forms divides into as many parts as that of nuclei. Each
two daughter cells. cytoplasmic part encircles one daughter nucleus.
b) Budding in yeast and budding in Hydra :-

S.N Budding in yeast budding in Hydra
1. Bud in Yeast is unicellular Bud in Hydra is a multicellular
2. Bud originates from a small protuberance Bud arises due to the repeated mitotic
on the parent body division
3. Yeast gets its daughter nuclei and it may or In Hydra the daughter buds are multi-cellular
may not separate from the parent body and there is no development of daughter bud
c) Regeneration in lizard and Planaria :- (March-2023)

S.N Lizard Planaria
1. Type of generation Epimorphosis Type of generation: Morphallaxis
2. In this type replacement of lost body parts occur In this type Whole body grows from a small fragment
13. How is juvenile phase different from reproductive phase? (March-2023)

S.N Juvenile phase Reproductive phase
1. It is a Time period between birth to just before It is an actual Reproductive phase
reproductive phase
2. This phase also known as primary growth phase This phase is known as secondary growth phase or
or vegetative phase sexual maturity
14. What is the difference between syngamy and fertilization ?

no Syngamy Fertilization
1. 1. The fusion of cells or their nuclei in reproduction. It is the process of fusion of dissimilar gametes
(maleand female gamets)
2. Syngamy term is used to denote fusion of cells occur Fertilization term is used to denote fusion of gamete
mostly in invertibrate or lower grade organisms occur in higher invertebrate and all vertebrates
ADDITIONAL QUESTIONS
1. What is paedogenetic parthenogenesis ?

 In this the larvae produce a new generation of larvae by parthenogenesis.
 Eg : Sporocysts and Reclia larvae of liver fluke.
2. What is strobilation ?
 In some metazoans, several transverse fissions occur simultaneously giving rise to a number of
individuals. E.g. Aurelia.

3. Explain encystment in amoeba :-
 During unfavorable conditions Amoeba withdraws its pseudopodia and secretes a three-layered, protective,
chitinous cyst wall around it and becomes inactive. This phenomenon is called encystment.
 When conditions become favourable, the encysted Amoeba divides by multiple fission and produces many
minute amoebae called pseudopodiospore or amoebulae.
 The cyst wall breaks off liberating the young pseudopodiospores.
4. During unfavourable conditions, how the Amoeba reproduces?

 During unfavorable conditions Amoeba reproduces by encystment.
 Amoeba withdraw its pseudopodia, and secrets 3 layers protective covering, chitinozes cyst wall around it
and becomes in active. It is called as encystment.
5. Difference Oviparous and Viviparous :-

Viviparous Oviparous
It is the type of development in which the young The young hatch from eggs laid outside the mother‟s
ones are born alive after being nourished in the body. Eg: Reptiles and Birds.
uterus through placenta . Eg :Mammals.
6. What are the types of natural parthenogenesis? Types are :-

1. Arrhenotohy :- Only makes are producer eg.honey bees.
2. Thelytohy :- Only female are produced. Eg.Solenobia
3. Amphitohy :- Egg may develop in to individuals of any sex.
7. Define Parthenogenesis & Mention its types :-

 Devlopment of an egg in to complete individuals without fertilization is called parthenogenesis.
Types: 1). Natural Parthenogenesis. 2). Artificial Parthenogenesis.
8. What is Senescent phase of life ? (May -2022)

 Senescent phase begins at the end of reproductive phase when degeneration sets in the structure and
functioning of the body.
9. Plasmotomy :- (March -2023)

 Plasmotomy is the division of multinucleated parent into many multinucleate daughter individuals with
the division of nuclei.
10. Write different types of syngamy :-

1. Autogamy - Male and female gametes are produced by the same cell or same organism and both the
gametes fuse together to form a zygote. e.g. Actinosphaerium and Paramecium.
2. Exogamy - the male and female gametes are produced by different parents and they fuse to form a zygote.
So it is biparental. e.g. Human – dioecious or unisexual animal.
3. Hologamy - In lower organisms, organisms itself behave as gametes and the fusion of such mature
individuals is known as hologamy. e.g. Trichonympha.

4. Paedogamy - union of young individuals produced immediately after the division of the adult parent
cell by mitosis.
5. Merogamy - fusion of small size and morphologically different gametes (merogametes).
6. Isogamy - it is the fusion of morphological and physiological identical gametes . e.g. Monocystis.
7. Anisogamy – it is the fusion of dissimilar gametes is called anisogamy. e.g. higher invertebrates and all
vertebrates.
Addition one word
1) Sporulation occurs in.____ Plasmodium
2) Which types of chromosomes are always present in gametes ? - Haploid
3) A single parent is enough for reproduction is called - Asexual
4) Amoeba reproduce by ---- Binary fission , Sporulation
5) An example of animal reproduce by multiple fission - Plasmodium.
6) During which process cyst is formed --- Sporulation.
7) An animal conjugation occurs as a sexual reproduction is - Paramoecium
8) Development of zygote taking place outside the body is called as - Oviparous
9) The type of division happens in Euglena - Longitudinal
10) In which of the following organism, internal bud formation seen in ----- Spongilla.
11) Which method of asexual reproduction can be said as method of regeneration ? – Fragmentation.
12) Which asexual reproduction three layer of protective wall develop ? - encystment .

2: HUMAN REPRODUCTION
BOOK BACK QUESTION
11. 17) Mention the differences between spermiogenesis and spermatogenesis :- (March -2023)
S.N Spermatogenesis Spermiogenesis

1. It is the process of formation of haploid s It is the process of differentiation of spermatozoon
spermatozoa from germinal cells from a spermatid
2. It involves conversion of a diploid structure It changes a haploid structure into another haploid
into haploid structures structure.
18) At what stage of development are the gametes formed in new born male and female?
1. Male - Spermatogenesis starts at the age of puberty. It is initiated by the release of Gonadotropin.
2. Female - At puberty secondary oocyte and ovum is formed from primary oocytes by miotic division.
19) Expand the acronyms :-

a) FSH – Follicle Stimulated hormone.
b) LH – Leutinising hormone
c) HCC – Human placental gonadotropin.
d) HPL – Human Placental Lactogen
20) How is polyspermy avoided in humans?

 Once fertilisation is happened cortical granules from the cytoplasm of the ovum form a barrier called
the fertilisation membrane around the ovum and preventing further penetration of other sperms.
 Thus polyspermy is prevented.
21) What is Colostrum? Write its significance .

 Colostrum is a yellowish, nutrient rich fluid produced by human female immediately after parturition.
 It is a natural antimicrobial agent stimulating the infant‟s immune system.
 It has less lactose. It has no fat. It has proteins, vitamin A, minerals.
 It is rich in IgA antibodies. It has immune, growth and tissue repair factors.
 It protects the injant‟s digestive tract against bacterial infection.
22) Placenta is an endocrine Tissue – Justify ?

 During pregnancy, the placenta acts as a temporary endocrine gland. It produces the following
hormones.
1. hCG - human Chorionic Gonadotropin, human chorionic somatomammotropin. (hCS)(or) human
placenal Lactogen (hPL) - supporting foetal growth.
2. Oestrogen , Progesterone , cortisol, prolactin, thyroxine - are essential for a normal pregnancy.
3. Relaxin - relax pelvic ligaments during parturition .

12. Draw a labelled sketch of a spermatozoan and explain the structure ? (March -2023)
 The human sperm is a flagellated, motile gamete.
 It is composed of head, neck and a tail.
 The head comprises of acrosome and nucleus.
 Acrosome is a helps to penetrate the ovum during Fertilization.
 The neck is very short and it contains the proximal centriole and the distal
centriole.
 The middle piece possesses mitochondria . It produces energy in the form of
ATP molecules for the movement of sperms.
 Tail is the longest part of the sperm and is slender and tapering.
23) What is Inhibin ? State its functions?

 Sertoli cell is in the stratified epithelium of seminuferous tubule, it secrete a hormone inhibin.
 It is involved in the negative feedback control of sperm production.
24) Mention the importance of the position of testes in humans?

 Testes are a pair of ovoid bodies lying in the scrotum.
 The scrotum is a sac of skin that hangs outside the abdominal cavity.
 Since viable sperms cannot be produced at normal body temperature.
 The scrotum is placed outside the abdominal cavity to provide a temperature 2-3oC lower than
the normal internal body temperature.
 Thus the scrotum acts as a thermoregulator for spermatogenesis.
25) What is the composition for semen? (May-2022)

 Semen is a milky white fluid with sperms and the seminal plasma.
 It is an alkaline fluid with fructose sugar, ascorbic acid, prostag land in, and vesiculase.
 Vericulase is a coagulating enzyme. It enhances sperm motility.
28) Define Gametogenesis ?

 Gametogenesis is the formation of gametes.
 Sperms and ova are produced from primary sex organs like testis and ovary.
 Meiosis plays a significant role in gametogenesis.
29) Describe the structure of the human ovum with a neat labelled diagram? (May -2022)
 Human ovum is microscopic, non - cleidoic and alecithal.
 It‟s cytoplasm is called Ooplasm. Ooplasm contains large nucleus called
germinal vesicle.
 It has outer thick coat of follicular cells called corona radiata.
 The middle thick layer is called zona pellucida.
 The inner thin transparent layer is called vitelline membrane.
 Between the vitelline membrane and zona pellucida is a narrow space called
perivitelline space.

30) Give a schematic representation of spermatogenesis and Oogenesis in humans?
13. Explain the various phases of menstrual cycle ? (March -2023)
Menstrual cycle:- It occurs in every 28/29 days. It is from puberty to menopause (except during pregnancy).
The cycle contains 4 phases.
1. Menstrual phase. (3-5 days)

 Progesterone, oestrogen level decreases.
 So uterine endometrial lining and the blood vessels break. It results in menstrual flow for 3 - 5 days.
 It occurs only if the ovum is not fertilised.
2. Follicular Phase (5 -14 days)
 Secretion of FSH and LH induces the following changes.
 Primary follicle of ovary becomes the mature graffian follicle.
 Endometrium regenerates. Follicular development is stimulated.
 Oestrogen is secreted by the follicle cells.

th
3. Ovulatory Phase (about 14 day)
 LH and FSH attain peak level.
 LH induces the rupture of graffian follicle Ovum (secondary Oocyte) is released from the ovary wall into
peritoneal cavity. Thisprocess is called Ovulation.
4. Luteal or Secretory Phase.
 The remaining part of the graafian follicle becomes a transitory endocrine gland. It is called corpus luteum.
 Corpus luteum secretes progesterone. It is needed for the maintenance of endometrium.
 After fertilisation the progesterone helps in implantation, of fertilised ovum.
 Uterine wall secretes nutritive fluid for the foetus. So this phase is called as secretory phase.
 During pregnancy all events of menstrual cycle stop and there is no menstruation .
 In the absence of fertilisation, the corpus luteum degenerates completely and leaves a scar tissue called
Corpus albicans.
32) Explain the role of oxytocin and relaxin in parturition and lactation?
1. Relaxin :-
 It relaxes pelvic joints and by dilation of the cervix with continued powerful contractions.
 The amnion ruptures and amniotic fluid flows through vagina.
 The placenta and the remains of the umbilical cord is called „after birth‟ is expelled out after delivery.
2. oxytocin :-
 It causes the ejection of milk from the alveoli of mammary glands.
 During lactation, it stimulates the contraction of the empty uterus. Now uterus return to prepregnancy size.
33) Identify the given image and label its parts marked as a,b,c and d
a. Vitelline Membrane
b. Nucleus.
c. Zona Pellucida.
d. Corona radiata.
34) The following is the illustration of the sequence of ovarian events (a.i) in a human female.
a) It represents ovulatory phase of Oogenesis.
b) Ovarian hormone - Progesterone, Oestrogen.

Pituitary hormone - FSH (Follicle stimulating hormone) LH (Luteinizing Hormone).

c) Change in uterus
1. Endometrium regenerates and fertilisation paves way for the implantation of fertilised ovum.
2. Uterine wall secretes nutritious fluid for the foetus. So luteal phase is called Secretory phase.
e) Write the difference between C - Secondary Follicle and H - Corpus luteum.
S.N Secondary follicle Corpus luteum

1. Found in follicular phase (or) proliferative phase. Found in luteal phase (or) secretory phase.
2. It is surrounded by many layers of granulosa cells It is a transitory endocrine gland secreting
and a new theca layer progesterone.
3. Later it becomes graafian follicle Later it becomes a scar called corpus albicans.
1). Mention the significance of Epididymis in the testis?

 It temporarily stores the sperm and it helps in physiological maturation of sperm.
 Sperms acquire increased motility and fertilising capacity.
2). What are the accessory glands in female reproductive system and its function ?
1) The Bartholin‟s gland –
It is posterior to the left and right of the opening of the vagina. Its secrete mucus to lubricate the vagina.
2) The Skene‟s glands –
They are located on the anterior wall of the vagina and around the lower end of the urethra. They secrete
a lubricating fluid.
3) What are Sertoli cells and its uses ?

 They are elongated and pyramidal and provide nourishment to the sperms till maturation.
 They also secrete inhibin hormone.
4) Roll of hormone in Spermatogenesis ?

1) Gonadotropin Releasing Hormone (GnRH) - initiated the puberty
2) Follicle Stimulating Hormone (FSH) - testicular growth and enhances the production of Androgen
3) Lutenizing Hormone (LH) – it acts on the Leydig cells and stimulates the synthesis of testosterone
which in turn stimulates the process of spermatogenesis.
5) Define : Menstrual or ovarian cycle :-

 It occurs approximately once in every 28/29 days during the reproductive life of the female from
menarche (puberty) to menopause except during pregnancy.
 Its is a cyclic changes occurs in the endometrium every month.
6) Menopause –define ?
 Menopause is the phase in a women‟s life when ovulation and menstruation stops.
 The average age of menopause is 45-50 years.
 It indicates the permanent cessation of the primary functions of the ovaries.

7) What is Ectopic pregnancy ?
 If the fertilized ovum is implanted outside the uterus it results in ectopic pregnancy.
 About 95 % of ectopic pregnancies occur in the fallopian tube.
 The growth of the embryo may cause internal bleeding, infection and in some cases even death due
to rupture of the fallopian tube.
8) Monozygotic (Identical) :- twins are produced when a single fertilized egg splits into two during the first
cleavage. They are of the same sex, look alike and share the same genes.
9) Dizygotic (Fraternal) :- twins are produced when two separate eggs are fertilized by two separate sperms.
The twins may be of the same sex or different sex and are non-identical.
10) Mayer – Rokitansky syndrome :- (March -2023).
 All women are born with ovaries, but some do not have functional uterus. This condition is called
Mayer-Rokitansky syndrome.
11) What is Let-Down” reflex ?

 Oxytocin causes the “Let-Down” reflexthe actual ejection of milk from the alveoli of the mammary glands.
5-MARK QUESTION
12) POLY CYSTIC OVARY SYNDROME (PCOS) :-
 PCOS is a complex endocrine system disorder that affects women in their reproductive years.
 Polycystic means „many cysts‟. It refers to many partially formed follicles on the ovaries, which contain
an egg each.
 But they do not grow to maturity or produce eggs that can be fertilized.
 Women with PCOS may experience irregular menstrual cycles, increased androgen levels, excessive
facial or body hair growth (hirsutism), acne, obesity, reduced fertility and increased risk of diabetes.
 Treatment for PCOS includes a healthy lifestyle, weight loss and targeted hormone therapy.
13) Discribe on extra embryonic membranes :- (May-2022)

 The extra embryonic membranes namely the amnion, yolk sac,
allantois and chorion.
 It protect the embryo from dessication, mechanical shock and help in
the absorption of nutrients and exchange of gases
1. Amnion :-
 Double layered, translucent membrane filled with amniotic fluid.
 Give buoyant environment to protect the embryo from injury.
 Regulate the temperature of foetus.
 Provide a medium for movement of foetus.
2. Yolk Sac :-
Forms a part of the gut and source of the earliest blood vessels and blood cells.
3. Allantois :-
 It is at the caudal end of yolk sac and small out pocketing of embryonic tissue.
 Structural base of umbilical cord and links embryo to the placenta. It becomes the part of the urinary bladder.
4. Chorion :-
 Outer most membrane and it encloses the embryo and all other membranes.
 Helps in the formation of placenta.

14) The major reproductive events in human beings are as follows:-
1) Gametogenesis - Formation of gametes by spermatogenesis and oogenesis.
2) Insemination - Transfer of sperms by the male into the female genital tract.
3) Fertilization - Fusion of male and female gametes to form zygote, called Fertilization.
4) Cleavage - Rapid mitotic divisions of the zygote which convert the single celled into a multicellular.
5) Implantation - Attachment of blastocyst to the uterine wall.
6) Placentation - Formation of placenta which is the intimate connection between foetus and uterine wall of
the mother for exchange of nutrients.
7) Gastrulation - Process by which blastocyst is changed into a gastrula with three primary germ layers.
8) Organogenesis - Formation of specific tissues, organs and organ systems from three germ layers.
9) Parturition - Expulsion of the foetus from the mother‟s womb.
15)
ONE WORD ANSWER
1) The male reproductive hormone Testosterone are secreted by – Leydig cells / Interstitial cells.
2) The viable sperms can be produced at temperature - 2 to 3oC.
3) The scrotum acts as a _____________for spermatogenesis - thermoregulator
4) The ovary remains attached to the pelvic wall by an ovarian ligament called – Mesovarium.
5) Which wall involve in cyclic changes during the menstrual cycle – Endometrium.
6) The spermatids are transformed into mature spermatozoa (sperms) by the process called – Spermiogenesis.
7) The sperm production remains nearly constant at a rate of about ________sperms per day- 200 million.
8) which proteolytic enzyme helps to penetrate the ovum during Fertilization – hyaluronidase.
9) It produces energy in the form of ATP molecules for the movement of sperms - Mitochondria.
10) The males produce more than ___________ sperms in their life time- 500 billion.
11) The smallest human cell - sperm
12) The largest human cell. - ovum or egg
13) Which type of egg in human ovum - Alecithal type.
14) The menstrual or ovarian cycle occurs approximately once in every - 28/29 days.
15) In female reproductive system which cell secrete the oestrogen - follicle cells.
16) The release of the ovum (secondary oocyte) from the ovary is called - ovulation.
17) The average age of menopause occur in - 45-50 years.
18) If the fertilized ovum is implanted outside the uterus it results in - Ectopic pregnancy.
19) Human pregnancy of the gestation period about - 280 days or 40 weeks.
20) Colostrum is also rich in ________ antibodies - IgA.
21) It refers to the failure of spermatogenesis - Azoospermia.
22) It is the first ejaculation of the semen - Spermarche.
-: GOD GRACE :-

3 : REPRODUCTIVE HEALTH
BOOK BACK QUESTION

9) What is amniocentesis? Why a statutory ban is imposed on this technique?
 Amniocentesis is generally performed in a pregnant woman between the 15th - 20th weeks of pregnancy.
 By inserting a long, thin needle through the abdomen into the amniotic sac to withdraw a small sample of
amniotic fluid. The amniotic fluid contains cells shed from the foetus.
 It helps to detect chromosomal abnormality in the foetus.
 It is misused to detect the sex of the foetus. It creates chance for female foeticide.
10) Select the correct term from the bracket and complete the given branching tree
A. Barriers
B. Lactational amonerrhoea
C. Tubectomy
D. CuT
11) Correct the following statements :-

a) Transfer of an ovum collected from donor into the fallopian tube is called ZIFT.
Ans :- Transfer an ovum collected from donor into the fallopian Tube – GIFT
b) Transfering of an embryo with more than 8 blastomeres into uterus is called GIFT.
Ans :- Transfer of the zygote with more than 8 blastomeres into the fallopian tube - ZEFT
c) Multiload 375 is a hormone releasing IUD.
Ans :- Multiload 375 is a copper releasing IUD.
12) Which method do you suggest the couple to have a baby, if the male partner fails to inseminate the female
or due to very low sperm count in the ejaculate?
 Intra Uterine Insemination (IUD) is suggested.
 This is a procedure to treat infertile men with low sperm count.
 Semen is collected from the husband or a healthy donor.
 Semen is introduced into the uterus through vagina by a catheter.
 Ovaries are stimulated to produce more ova.
 Sperms swim towards the fallopian tubes and fertilize the egg, resulting in normal pregnancy.
13) Expand the following :-
a) ZIFT - Zygote Intra - Fallopian Transfer.
a) ICSI - Intra Cytoplasmic Sperm Injection.

14) What are the strategies to be implemented in India to attain total reproductive health? (May-2022)
1. Family Planning programme since 1951 and is periodical assessed every decade.
2. Reproductive and child Health care (RCH) programmes.
3. Creating awareness and providing medical assistance to build a healthy society.
4. Introducing sex education in schools to provide information about adolescence.
5. Educating couples and those in the marriageable age groups about the available birth control methods.
6. Awareness about care for pregnant women post natal care of mother and child.
7. Encouraging the government and non-governmental agencies to identify new birth control methods.
15) Differentiate foeticide and infanticide. :- (May-2022)

Foeticide Infanticide
Foeticide Aborting the female in the mother's womb Infanticide Killing the female child after her birth.
19) The procedure of GIFT involves the transfer of female gametes into the fallopain tube, can gametes be
transferred to the uterus to achieve the same result? Explain.
 This is a procedure (IUI) to treat infertile men with low sperm count.
 The semen is collected either from the husband or from a healthy donor.
 And it is introduced into the uterus through the vagina by a catheter.
 After stimulating the ovaries to produce more ova.
 The sperms swim towards the fallopian tubes to fertilize the egg, resulting in normal pregnancy.
20) Amnicentesis, the foetal sex determination test, is banned in our country, Is it necessary? comment.
 The ban of Amniocentesis is necessary in our country. Because :-
 It is misused to detect the sex of the foetus. It creates chance for female foeticide.
21) Open Book Assessment

Healthy reproduction, legally checked birth control measures and proper family planning
programmes are essential for the survival of mankind' Justify.
1. Healthy reproduction - is a state of well being in all aspects of reproduction, so as to make the people
contribute to a healthy society.
2. Birth control or family planning - can help people design their family small and healthy and their
reproductive life smooth and happy.
3. To restrict population growth and to keep small family, the skewed choice of male child over female is done.
4. So by giving awareness to the school children.
5. The coming society will be Reproductive Healthy, a criteria essential for the survival of mankind.
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1. What are the health & the social benefits of family planning?
 Protecting the health of women by reducing frequent pregnancies
 Reducing abortions.
 Providing adequate time lapse between successive pregnancies.
2. Importance of POCSO Act – (May-2022)
 Prevention of children from sexual offences.
 Sexual harassment at workplace – (Prevention, prohibition and redressal) Act.
3. Difference between vasectomy & Tubectoncy :-
s.n Vasectomy Tubectomy
1. It is a surgical procedure for male sterilization It is the surgical sterilization in woman.
2. Both vas deference are cut and tied through on scrotum Oviduct is cut and tied through vagina
3. It prevents the entry of sperm in to the urethra It prevent the fertilization
4. Zygote intra-fallopian transfer (ZIFT) :-

 As in IVF, the zygote upto 8 blastomere stage is transferred to the fallopian tube by laparoscopy.
 The zygote continues its natural divisions and migrates towards the uterus where it gets implanted.
5. Gamete intra-fallopian transfer (GIFT) :-

 Transfer of an ovum collected from a donor into the fallopian tube.
 In this the eggs are collected from the ovaries and placed with the sperms in one of the fallopian tubes.
 The zygote travels toward the uterus and gets implanted in the inner lining of the uterus.
6. Write a short note on cervical cancer.

1. Definition: It is the abnormal growth of cervical cells earned by STV namely Human Papilloma Virus
2. Symptoms: Pelvic pain, increased vaginal discharge & abnormal vaginal bleeding.
3. Risk factors: Prolonged use of contraceptive pills, having multiple sexual partners.
4. Diagnosis: Pap smear
5. Prevention: modification of life style, healthy diet, avoiding tobacco.
7. Chorionic villus sampling (CVS) :- It is a prenatal test that involves taking a sample of the placental tissue
to test for chromosomal abnormalities.
8. Write three causes of infertility :-
1) Undescended testes and swollen veins in scrotum.
2) Tight clothing in men may raise the temperature in the scrotum & affects production.
3) Females may develop antibodies against her partner‟s sperm.
9. What is Surrogacy :-
 It is a method of assisted reproduction or agreement whereby a woman agrees to carry a pregnancy for
another person, who will become the newborn child's parent after birth.
14. Suggest a solution for the prevention of male infertility to the patients with Azoospermia?
 Micro- testicular sperm extraction is suggested (TESE).
 Sperm is removed from the testis by surgery.
 Incision is made in scrotum , Seminiferous tubules are dilated.
 Testicular tissue are removed from the area of active sperm production.

11. The various steps involve in the Invitrofertilization (IVF) or Test Tube Baby?
1) Ovarian stimulation - Human chorionic Gonadotropin (hcG) is injected.
2) Eggs are prepared and stripped from the sorrounding cells.
3) Sperm is prepared by special media. Sperms and eggs are brought together.
4) Then the zygote is allowed to divide to form 8 celled blastomere.
5) Then transferred into the uterus for a successful pregnancy.
6) The transfer of an embryo in 8 cell stage into uterus is called Embryo Transfer Technique.
12. Foetoscope :-
 It is used to monitor the foetal heart rate and other functions during late pregnancy and labour.
 The average foetal heart rate is between 120 and 160 beats per minute.
 An abnormal foetal heart rate or pattern may mean that the foetus is not getting enough oxygen and it
indicates other problems.
 A hand-held doppler device is often used during prenatal visits to count the foetal heart rate.
 During labour, continuous electronic foetal monitoring is often used.
ONE WORD
1. It is known as anti-sterility vitamin as it helps inthe normal functioning of reproductive structures –Vitamin E
2. Sex hormones were discovered by - Adolf Butenandt.
3. World Population Day - 11th July .
4. World AIDS Day - 1st December .
5. NACO (National AIDS Control Organisation) was established in - 1992.
6. They are commonly called as international diseases - Syphilis and gonorrhoea.
7. The world to initiate the „Family planning programme‟ since - 1951.
8. It is refers to „aborting the female in the mother‟s womb - Female foeticide.
9. It is „killing the female child after her birth - infanticide .
10. Prevention of children from sexual offences act is - POCSO Act.
11. This delay in ovarian cycles is called - lactational amenorrhoea.
12. All women are born with ovaries, but some do not have functional uterus it is called Mayer-Rokitansky
syndrome.
-: GOD GRACE :-

4 : Principles of Inheritance and Variation
BOOK BACK QUESTION

22) What is haplodiploidy?
 The sex of the off spring is determind by the numbers of sets of chromosomes ex : Honey bee, wasp,Ants
 Fertilized egg develops into females. (Queen / worker honeybee).
 Unfertilised eggs develop into males (drones) by parthenogenesis.
 The males have half the number of chromosomes (haploid).
 The Females have double the number (diploid)
 Hence the sex determination is called haplodiploidy. It is seen in hymenopteran insects.
23) Distinguish between heterogametic and homogametic sex determination systems.

Heterogametic. Homogametic
The individuals (male) produce two types of gametes (X,Y),
Individuals (female ) produce only one type (X,X) of gamete.
so they are said to be heterogametic. So they are said to be homogametic.
16) What is Lyonisation? (Lyon‟s hypothesis / Dosage compensation Barr body) (March -2023)
 In the XY Chromosomal system of sex determination, males have only one X Chromosome and the
females have two X Chromosomes.
 This condensed body was called sex chromatin by them, and reffered as Barr body.
 In females, these X Chromosome becomes tightly coiled into a heterochromatin (Lyon‟s hypothesis).
 The number of Barr bodies observed in cell was one less than the number of X-chromosome.
 XO female have no Barr body, whereas XXY males have one Barr body.
24) What is criss-cross inheritance?.OR ( colour blind man X normal visioned woman ) (Annual -2020)
OR ( Sex linked characters in human being )
 A marriage between a colour blind man and a normal visioned
woman.
 It will produce normal visioned male and female individuals in F1
generation but the females are carriers.
 The marriage between a F1 normal visioned carrier woman and a
normal visioned male will produce:
1. one normal visioned female,
2. one carrier female,
3. one normal visioned male and
4. one colour blind male.
 Colour blind trait is inherited from the male parent to his grandson
through carrier daughter.Which is an example of criss-cross
pattern of inheritance.
 “ This type of inheritance of recessive sex linked character from
father to daughter and then from the daughter to her sons” is
known as criss cross inheritance.

25) Why are sex linked recessive charactersmore common in the male human beings?
 The X–linked genes have no corresponding alleles in the Y chromosome.
 The Y linked genes have no corresponding allele in X chromosome.
 The Y linked genes inherit along with Y chromosome and they phenotypically express only in the male.
 Sex linked inherited traits are more common in males than females because, males are hemizygous and
therefore express the trait when they inherit one mutant allele.
26) What are holandric genes?

 The genes present in the differential region of Y chromosome are called Y- linked or holandric genes.
 Y linked genes inherit along with y-chromosome and phenotypically express only in the male.
27) Mention the symptoms of Phenyl ketonuria.

 Mental Retardation.
 Light pigmentation of skin and hair
 Phenylpyruvic acid is excreted in urine
28) Mention the symptoms of Downs syndrome / ( 21-Trisomy) :- (Annual -2020)

 Trisomic condition of chromosome – 21 results in Down‟s syndrome.
 It is characterized by severe mental retardation, defective development of the central nervous
system,
 Increased separation between the eyes, flattened nose .
 Ears are malformed, mouth is constantly open and the tongue protrudes.
29) Explain the genetic basis of ABO blood grouping man.

 Three autosomal alleles are on chromosome 9.
 These alleles determine the blood group.
 The gene for blood group is labeled as „I‟ (I - Isoagglu-
tinogen - antigen)
 I gene has 3 allelic forms IA IB IO
 IA specifies A antigen , IB for B antigen
 IO allele specifies no antigen.
 The phenotypic combinations is –A,B,AB, and O.
 The genotypes are IAIA ,IAIO , IB IB,IBIO, IAIB, and IOIO
30) How is sex determined in human beings ? (MAY-2022)

 Gene determining sex in human beings are located in sex chromosomes called allosomes.
 Sex determination is based on chromosomal differences between male and female.
 Females have XX chromosomes and Males have XY chromosomes.
 Females are homogametic. They produce only one type (X) gamete.
 Males are heterogametic. They produce 2 types (X,Y) of gamete (sperms).
 The egg fertilised by X sperm produce female.
 The egg fertilised by Y sperm produce male.

31) Explain male heterogamety.
 In this method of sex determination the males are heterogametic producing dissimilar gametes.
 It is of two kinds XX-XO type and XX-XY type.
 XX-XO of sex determination is seen in bugs, some insects such as cockroaches and grasshoppers.
 XX-XY of sex determination is seen in human beings and in Drosophila.
32) Brief about female heterogamety. –

 In this method of sex determination, the homogametic male possesses two „X‟ chromosomes as in
certain insects and certain vertebrates like fishes, reptiles and birds producing a single type of gamete;
while females produce dissimilar gametes.
 The female sex consists of a single „X‟ chromosome or one „X‟ and one „Y‟ chromosome.
 Thus the females are heterogametic and produce two types of eggs.
 To avoid confusion with the XX-XO and XX-XY types of sex determination, the alphabets „Z‟ and „W‟
are used here instead of X and Y respectively.
 Heterogametic females are of two types, ZO-ZZ type and ZW-ZZ type.
33) Give an account of genetic control of Rh factor (or) Fisher and Race hypothesis:-
 Rh factor involves 3 different pairs of alleles located on 3 different closely linked loci
on the chromosome pair.
 This system is more commonly in use today, and uses the 'Cde' nomenclature.
 The three pairs of Rh alleles (Cc, Dd and Ee) occur at 3 different loci on homologous
chromosome pair-1.
 The possible genotypes will be one C or c, one D or d, one E or e from each
chromosome.
 For e.g. CDE/cde; CdE/cDe; cde/cde; CDe/CdE etc.,
 All genotypes carrying a dominant „D‟ allele will produce Rh+positive phenotype.
 The double recessive genotype „dd‟ will give rise to Rh-negative phenotype.

34) Explain the mode of sex determination in honeybees.
 In honeybees mechanism of sex determination called haplodiploidy mechanism.
 In this system, the sex of the offspring is determined by the number of sets of chromosomes it receives.
 Fertilized eggs develop into females (Queen or Worker) and unfertilized eggs develop into males (drones)
by parthenogenesis.
 It means that the males have half the number of chromosomes (haploid).
 The females have double the number (diploid).
 Hence the name haplodiplody for this system of sex determination in honey bees.
17) What are the applications of Karyotyping ?. (March -2023)

1. It helps in gender (male and female) identification.
2. It is used to detect the chromosomal aberrations like deletion, duplication, translocation, nondisjunction
of chromosomes.
3. It helps to identify the abnormalities of chromosomes like aneuploidy.
4. It is also used in predicting the evolutionary relationships between species.
5. Genetic diseases in human beings can be detected by this technique.
35) Explain the inheritance of sex linked characters in human being.

 Inheritance of traits by genes located in sex chromosomes is called sex linked Inheritance.
 Genes in the differential region of X chromosome are called X linked genes.
 Genes in the differential region of Y chromosome are called Y linked genes (or) Holandric genes.
 The Examples for X - linked inheritance are Colour blindness , Haemophila
ADDITIONAL QUESTION
1. Wiener Hypothesis :-
 Wiener proposed the existence of eight alleles (R1, R2, R0, Rz, r, r1, r11, ry) at a single Rh locus.
 All genotypes carrying a dominant „R allele‟ (R1, R2 ,R0 ,Rz).
 Will produce Rh+positive phenotype and double recessive genotypes (rr, rr1, rr11, rry) will give rise to
Rh-negative phenotype.
2. Kin Selection :-
 All other females in honey bees which are diploid having developed from fertilized eggs help to raise the
queen‟s eggs.
 So contribute to the queen‟s (honey bees) reproductive success and indirectly to their own, a phenomenon
known as Kin Selection.
3. Haemophilia :-
 Haemophilia is commonly known as bleeder‟s disease, which is more common in men than women.
 This hereditary disease was first reported by John Cotto in 1803.
 Haemophilia is caused by a recessive X-linked gene.
 A person with a recessive gene for haemophilia lacks a normal clotting substance (thromboplastin)
in blood, hence minor injuries cause continuous bleeding, leading to death.
 The females are carriers of the disease and would transmit the disease to 50% of their sons even if the
male parent is normal.
 Haemophilia follows the characteristic criss - cross pattern of inheritance.

4. The prevent erythroblastosos foetalis – (May -2022)
 If the mother is Rh negative and foetus is Rh positive, anti D antibodies should be administered to the
mother at 28th and 34th week of gestation as a prophylactic measure.
 If the Rh negative mother delivers Rh positive child then anti D antibodies shouldbe administered to the
mother soon after delivery.
 This develops passive immunity and prevents the formation of anti D antibodies in the mothers blood by
destroying the Rh foetal RBC before the mother‟s immune system is sensitized. This has to be done
whenever the woman attains pregnancy.
5. Karyotyping – Idiogram :-
 It is a technique through which a complete set of chromosomes is separated from a cell and the
chromosomes are arranged in pairs.
 The individual chromosomes are cut from the photograph and are arranged in an orderly fashion in
homologous pairs. This arrangement is called a karyotype.
 An Idiogram refers to a diagrammatic representation of chromosomes.
6. Pedigree analysis :-
 Pedigree is a “family tree”, drawn with standard genetic symbols, showing the inheritance pathway for
specific phenotypic characters.
 Pedigree analysis is the study of traits as they have appeared in a given family line for several past
generations.
7. Thalassemia :-
 Thalassemia is an autosomal recessive disorder.
 It is caused by gene mutation resulting in excessive destruction of RBC‟s due to the formation of
abnormal haemoglobin molecules resulting in anaemia.
 Normally haemoglobin is composed of four polypeptide chains, two alpha and two beta globin
chains.
 Thalassemia is classified into alpha and beta based on which chain of haemoglobin molecule is
affected.
 It is controlled by two closely linked genes HBA1 and HBA2 on chromosome 16.
 Mutation or deletion of one or more of the four alpha gene alleles causes Alpha Thalassemia.
 In Beta Thalassemia, production of beta globin chain is affected.
 It is controlled by a single gene (HBB) on chromosome
8. Phenylketonuria :-
 It is an inborn error of Phenylalanine metabolism caused due to a pair of autosomal recessive genes.
 It is caused due to mutation in the gene PAH located on chromosome12.
 This enzyme is essential for the conversion of phenylalanine to tyrosine.
 Lacks this enzyme, so phenylalanine accumulates and gets converted to phenylpyruvic acid .
 It is characterized by severe mental retardation, light pigmentation of skin and hair. Phenylpyruvic
acid is excreted in the urine.

9. Albinism :-
o It is an inborn error of metabolism, caused due to an autosomal recessive gene.
o Melanin pigment is responsible for skin colour. Absence of melanin results in a condition called
albinism.
o A person with the recessive allele lacks the tyrosinase enzyme system, which is required for the
conversion of dihydroxyphenyl alanine (DOPA) into melanin pigment inside the melanocytes.
o In an albino peoples have lack of melanin pigments in their skin, hair, iris, etc.
10. Huntington‟s chorea:-

 It is inherited as an autosomal dominant lethal gene in man.
 It is characterized by involuntary jerking of the body and progressive degeneration of the nervous
system, accompanied by gradual mental and physical deterioration.
 The patients with this disease usually die between the age of 35 and 40.
11. Patau‟s Syndrome/Trisomy-13 :-

 Trisomic condition of chromosome 13results in Patau‟s syndrome.
 Meiotic non disjunction is thought to be the cause for this chromosomal abnormality.
 It is characterized by multiple and severe body malformations as well as profound mental deficiency.
 symptoms are small head with small eyes, cleft palate, malformation of the brain and internal organs.
12. Klinefelter‟s Syndrome (XXY Males) :-

 This genetic disorder is due to the presenceof an additional copy of the X chromosome resulting in a
karyotype of 47,XXY.
 Persons with this syndrome have 47 chromosomes (44AA+XXY).
 They are usually sterile males, tall, obese, with long limbs, high pitched voice, under developed
genitalia and have feeble breast (gynaecomastia) development.
13. Turner‟s Syndrome (XO Females) :-

 This genetic disorder is due to the lossof a X chromosome resulting in a karyotype of 45+X.
 Persons with this syndrome have 45 chromosomes (44 autosomes and one X chromosome)
(44AA+XO) and are sterile females.
 Low stature, webbed neck, under developed breast, rudimentary gonads lack of menstrual cycle
during puberty, are the main symptoms of this syndrome.

5 : Molecular Genetics
BOOK BACK QUESTION

18) Give reasons: Genetic code is „universal‟:-
 The genetic code is universal. It means that all known living systems use nucleic acids and the same
three base codons (triplet codon) direct the synthesis of protein from amino acids.
 For example, the mRNA (UUU) codon codes for phenylalanine in all cells of all organisms.
19) Name the parts marked „A‟ and „B‟ in the given transcription unit :-
A - Promoter
B - Coding strand
20) Differentiate - Leading stand and lagging strand :-

Leading strand Lagging strand (March -2023)
1. The strand grows continuously without any gap Its growth is discontinuous synthesized fragments of
the lagging strand called as Okazaki fragments.
2. The direction of growth of the leading strand The direction of growth is 3‟5‟
is 5‟ 3‟
3. It is a rapid process It is a slower process
4. Only one single RNA primer is required More number of RNA primer is required.
21) Differentiate - Template strand and Coding strand :- (MAY-2022)

S.N Template strand Coding strand
1. Template strand is directed in the 5‟3‟ direction Coding strand is directed in the 3‟ 5‟ direction
2. Transcribed into mRNA Not transcribed into mRNA
3. Contains anti codons Contains codons
4. Contains the same nucleotide sequence as the Contains the complementary nucleotide sequence
rRNA as the tRNA
19) Mention any two ways in which single nucleotide polymorphism (SNPs) identified in human genome can
bring revolutionary change in biological and medical science.?
 Scientists have identified about 1.4 million locations where single base DNA differences
(SNPs – Single nucleotide polymorphism – pronounce as „snips‟) occur in humans.
 Identification of „SNIPS‟ is helpful in finding chromosomal locations for disease associated sequences
and tracing human history.
20) State any three goals of the human genome project. (May-2022)
1. Identify all the genes (approximately 30000) in human DNA.
2. Determine the sequence of the three billion chemical base pairs that makeup the human DNA.

3. To store this information in databases and Improve tools for data analysis.
21) In E.coli, three enzymes β- galactosidase, permease and transacetylase are produced in the presence of lactose.
Explain why the enzymes are not synthesized in the absence of lactose.
 A repressor protein binds to the operator region of the operon and prevents translation, as a result,
β-galactosidase is not produced.
 In the absence of preferred carbon source such as glucose, if lactose is available as an energy source for
the bacteria then lactose enters the cell as a result of permease enzyme.
 Lactose acts as an inducer and interacts with the repressor to inactivate it.
 The repressor protein binds to the operator of the operon and prevents RNA polymerase from
transcribing the operon.
 In the presence of inducer, such as lactose or allolactose, the repressor is inactivated by interaction
with the inducer.
 This allows RNA polymerase to bind to the promotor site and transcribe the operon to produce lac
mRNA which enables formation of all the required enzymes needed for lactose metabolism.
22) Distinguish between structural gene, regulatory gene and operator gene.
1. Structural genes (z,y,a) - it has 3 genes namely lac z,y and lac a. The lac „z‟ gene codes for β-galactosidase,
lac „y‟ gene codes for permease and „a‟ gene codes for transacetylase.
2. Regulatory gene (i) - the „i‟ gene transcribes a repressor mRNA and after its translation, a repressor protein
is produced.
3. Promoter (p) - In lac operon, a polycistronic structural gene is regulated by a common promoter and
regulatory gene.
4. Operator gene (o) - The repressor protein binds to the operator of the operon and prevents RNA polymerase
from transcribing the operon. This allows RNA polymerase to bind to the promotor site and transcribe the
operon to produce lac mRNA.
23) A low level of expression of lac operon occurs at all the windows for treatment of various genetic disorders.
Justify the statement. ?
 Lactose present in the external medium can enter the bacterium only when the bacterium contain the
enzyme permease.
 So, formation of permease require a low level expression of lac – operon.
24) HGP is the windows for treatment of various genetic disorders, justify the statement.
 To examine a persons DNA and to identify genetic abnormalities
 To diagnose disease and provide genetic counseling
 To solve challenges in healthcare.
25) Why the human genome project is called a mega project ?.

1. The international human genome project was launched in the year 1990.
2. It was a mega project and took 13 years to complete.
3. The human genome is about 25 times larger than the genome of any organism.
4. Human genome is said to have approximately 3×109 bp.
26) From their examination of the structure of DNA, What did Watson and Crick infer about the probable
mechanism of DNA replication, coding capability and mutation?

 Semi-conservative replication was proposed by Watson and Crick in 1953.
 This mechanism of replication is based on the DNA model.
 They suggested that the two polynucleotide strands of DNA molecule unwind and start separating at
one end. During this process, covalent hydrogen bonds are broken.
 The separated single strand then acts as template for the synthesis of a new strand.
 Subsequently, each daughter double helix carries one polynucleotide strand from the parent molecule
that acts as a template and the other strand is newly synthesised and complementary to the parent
strand.
27) Why tRNA is called an adapter molecule?

 The transfer RNA, (tRNA) molecule of a cell acts as a vehicle that picks up the amino acids scattered
through the cytoplasm.
 It reads specific codes of mRNA molecules. Hence it is called an adapter molecule. This term was
postulated by Francis Crick.
28) What are the three structural differences between RNA and DNA ?
No DNA RNA
1. It is double stranded It is single stranded
2. Deoxy ribose sugar is present Ribose sugar is present
3. Adenine, guanine, cytosine and thyamine Adenine, guanine, cytosine & Uracil
4. Chemically & Structurally less stable Chemically & Structurally more stable
29) Name the anticodon required to recognize the following codons:

Codons AAU CGA UAU GCA
Anticodon UUA GCU AUA CGU
30) a) Identify the figure given below
 DNA REPLICATION
b) Redraw the structure as a replicating fork and label the parts :-
c) Write the source of energy for this replication and name the enzyme involved in this process.
 Deoxy nucleotide triphosphate acts as substrate and also provides energy for polymerization reaction.
 DNA helicase, DNA ligase, DNA polymerase – these enzymes are involved in this process.

d) Mention the differences in the synthesis of protein, based on the polarity of the two template strands.
1. If both the strands act as a template, they would code for RNA with different sequences. This in turn
would code for proteins with different amino acid sequences. This would result in one segment of DNA
coding for two different proteins, hence complicate the genetic information transfer machinery.
2. If two RNA molecules were produced simultaneously, double stranded RNA complementary to each
other would be formed. This would prevent RNA from being translated into proteins.
31) If the coding sequence in a transcription unit is written as follows:

The Sequence of DNA : 5' TGC ATG CAT GCA TGC ATG CAT GCA TGC 3'
The sequence of mRNA: 3‟ACG UAC GUA CGU ACG UAC GUA CGU ACG 5‟
32) How is the two stage process of protein synthesis advantageous?
1. Transcription (May-2022)- It is the process of copying genetic information from one strand of DNA
into RNA. This process takes place in presence of DNA dependent RNA polymerase.
2. Translation – transcribed mRNA canbe translated into Proteins.
33) Why did Hershey and Chase use radioactively labelled phosphorous and sulphur only? Would they have got
the same result if they use radiolabelled carbon and nitrogen?
 All nucleic acids contain phosphorus , but not in protein.
 All protein contain sulphur but not in nucleic acids.
 So they designed an experiment using radioactive isotopes of Sulphur ( 35S ) and phosphorus ( 32P ) to
keep separate track of the viral protein and nucleic acids during the infection process.
 They haven‟t got the same result if they used radio labelled carbon and nitrogen.Because :
 Carbon is an organic molecules found in all cells.
 Nitrogen is structural molecules found in proteins, cell membrane and DNA.
34) Explain the formation of a nucleosome :-

 The DNA double helix length 2.2 m., is much morecomplex.
 Chromatin consists of repeating units called nucleosomes.
 Two molecules of the 4 histone proteins H2A, H2B, H3 and H4 are organized to form a unit of 8
molecules called histone octamere.
 The negatively charged DNA is wrapped around, the positively charged histone octamere to form a
structure called nucleosome.
 Nucleosome contains 200 bp of DNA helix and it is coiled on the outside of nucleosome.
 Adjacent nucleosomes are connected by linker DNA(H1) that is exposed to enzymes.
 The DNA makes 2 complete turns around the histone octameres and the two turns are sealed off by an
H1 molecule.Chromatin without H1 has a beads-on-a-string appearance in which DNA enters and
leaves the nucleosomes at random places.
 Further folding is due to interaction between H1 of one nucleosomes. DNA is a solenoid and packed
about 40 folds.

35) It is established that RNA is the first genetic material. Justify giving reasons.
 A typical cell contains about 10 times as much RNA as DNA.
 The high RNA play a variety of roles in the cell.
 Fraenkel-Conrat and Singer (1957) first demonstrated that RNA is the genetic material in TMV virus.
 In the early 1980‟s ( Leslie Orgel, Francis Brick and Carl Woese-3 molecular biologists) proposed the
„RNA world‟ as the first stage in the evolution of life.
 RNA catalysed all molecules necessary for survival and replication.
 The term „RNA world‟ first used by Walter Gilbert in 1986.
 There is now enough evidence to suggest that essential life processes (such as metabolism, translation,
splicing etc.,) evolved around RNA.
 RNA has the ability to act as both genetic material and catalyst.
 This hypothesizes reveals that RNA as the first genetic material on earth.
 Andrew Fire and Craig Mellow (recipients of Nobel Prize in 2006) were of the opinion that RNA is an
active ingredient in the chemistry of life.
ADDITIONAL QUESTION
1. One gene-one enzyme hypothesis :-
 George Beadle & Edward Tatum in the early 1940‟s on Neurospora led them to proposed this hypothesis.
 which states that one gene controls the production of one enzyme.
2. One gene-one polypeptide hypothesis :-

 It was observed that an enzyme may be composed of more than one polypeptide chain and a gene can code
for only one polypeptide chain.
 One gene controls the production of only one polypeptide chain of an enzyme molecule.
3. Difference between the Euchromatin – Hetero chromatin :-
No Euchromatin Hetero chromatin
1. In a typical nucleus, some regions of chromatin The chromatin that is tightly packed (stained
are loosely packed (lightly stained) darkly)
2. Transcriptionally active Transcriptionally inactive.
4. What are genetic codon?

 The genetic codon is a triplet code and 61 codons code for amino acids.
 Three codons do not code any amino acid and act as stop codon.
5. What are Operons ? How many operon groups are present in E.coli ? ( March -2020)
 The clusters of gene with related functions are called operons.
 75 different operons present in E.coli.

6. What are the uses of HGP.
1) To examine a persons DNA and to identify genetic abnormalities
2) To diagnose disease and provide genetic counseling
3) To solve challenges in healthcare, agriculture, energy and environmental remediation
7. What are the advantages of DNA finger printing. ( May-2022)

1) Forensic analysis: It can be used in identification of a person involved in criminal activities
2) Pedigree analysis: Inheritance pattern of genesthro generation and to detect inherited of diseases.
3) Conservation of wild life: to protect endangered species.

8. What is pharmacogenomics?
 This is a new field which combine pharmacology and genomics.
 This is effective in developing safe medication.
9. The salient features of genetic code :- (May -2022)

1) The genetic codon is a triplet code and 61 codons code for amino acids and 3 codons do not code for any
amino acid and function as stop codon (Termination).
2) The genetic code is universal. It means that all known living systems use nucleic acids
3) A degenerate code means that more than one triplet codon could code for a specific amino acid.
For example, codons GUU, GUC, GUA and GUG code for valine.
4) Non-ambiguous code means that one codon will code for one amino acid.
5) The code is always read in a fixed direction i.e. from 5'→3' direction called polarity.
6) AUG has dual functions. It acts as a initiator codon and also codes for the amino acid methionine.
7) UAA, UAG (tyrosine) and UGA (tryptophan) codons are designated as termination (stop) codons and also
are known as “non-sense” codons.
10. Write about the Salient features of HGP (MAY-2022) + (March -2023)
1) The human genome contains 3 billion nucleotide bases.
2) An average gene consists of 3000 bases, the largest known human gene being dystrophin with
2.4 million bases.
3) Genes are distributed over 24 chromosomes. Chromosome 19 has the highest gene density.
Chromosome 13 and Y chromosome have lowest gene densities.
4) The chromosomal organization of human genes shows diversity.
5) There may be 35000 - 40000 genes in the genome and almost 99.9 nucleotide bases are exactly the
same in all people.
6) Functions for over 50 percent of the discovered genes are unknown.
7) Less than 2 percent of the genome codes for proteins.
8) Repeated sequences make up very large portion of the human genome. Repetitive sequences have no
direct coding functions but they shed light on chromosome structure, dynamics and evolution
(genetic diversity).
9) Chromosome 1 has 2968 genes, whereas chromosome ‟Y‟ has 231 genes.
10) Scientists have identified about 1.4 million locations where single base DNA differences (SNPs –
Single nucleotide polymorphism – pronounce as „snips‟) occur in humans. Identification of„SNIPS‟
is helpful in finding chromosomal locations for disease associated sequences and tracing human
history.
11. Structure of RNA :- (fig. refer – practical guide ) (March -2023)

 tRNA molecule of the cell acts as a vehicle that pick up the amimo acids scattered through the cytoplasm.
 The secondary structure of tRNA depicted in looks like a clover leaf.
 In actual structure, the tRNA is a compact molecule which looks like an inverted “ L “
 The clover leaf model of tRNA shows the presence of three arms namely DHU arm, Middle arm
and TC arm.
 These arms have loops such as amino acyl binding loop, anticodon loop and ribosomal binding loop at
their ends.
 In addition, it also shows a small lump called variable loop or extra arm.
 The amino acid is attached to one end and the other end consists of 3 anticodon nucleotides.

6 : EVOLUTION
BOOK BACK QUESTION

17. List out the major gases seems to be found in the primitive earth. (May-2022)
 Ammonia, methane, hydrogen and water vapour.
 The atmosphere was oxygen free and the combination of CO2, NH3 .
18. Explain the three major categories in which fossilization occur ?

1. Actual remain.
 The original hard parts such as bones teech or shells are preserved as such in the earths atmosphere.
 This is the most common method of fossilization.
 When marine animals die, their hard parts such as bones, shells etc. covered with sediments and are
protected from further deterioration.
2. Petrification
 When the animal die the original parts of their body may be replaced molecule minerals and the orginal
substance being lost through disintegration.This method of fossilization is called petrifaction.
 The principle minerals involved in this type fossilization are iron pyries, silica, calcium carbonate and
bicarbonates of calcium and magnesium.
3. Natural moulds and casts
 Even after disintegration, the body of an animals might leave indelible impression on the soft mud which
later becomes hardened into stones. Such impressions are called moulds.
 The cavities of the moulds may get filled up by hard minerals and get fossilized which are called casto.
 Hardened faecal matter termed as coprolites occur as tiny pellets.
 Analysis of the coprolities enables us to understand the nature of diet the pre historic animals thrived on.
19. Differentiate between divergent evolution and convergent evolution with one example for each. (March -2023)
No Divergent evolution Convergent evolution
1. Structures which are similar structural but Organisms having different structural patterns
perform different functions are called but similar function are termed as analogous
homologous structures that brings about structures.
divergent evolution.
2. Fore limbs of vertebrates exhibit anatomical The wings of birds and insects are different
similarity with each other and is made of structurally but perform the same function of
similar bones such as humerus, radius, ulna, flight that brings about convergent evolution.
carpals, metacarpals and phalanges.
20. How does Hardy-Weinberg‟s expression (p2+2pq+q2=1) explain that genetic equilibrium is maintained in a
population? List any four factors that can disturb the genetic equilibrium.
 The allele frequencies in a population are stable and are constant from generation to generation in
the absence of gene flow, genetic drift, mutation recombination and natural selection.
 Evolution is a change in the allele frequencies in a population over time. Hence population in hardy
Weinberg is not evolving.

 Explain the equilibrium :-
 A large population of beetles appear in two colours dark grey(black) and light grey and their colour is
determined by „A‟ gene „AA‟ and „Aa‟ beetles are light grey.
 „A‟ allele has frequency (P) of 0.3 and „a‟ allele has a frequency (q) of 0.7 Then p + q = 1
 If a population is in hardy Weinberg equilibrium, the genotype frequency can be estimated by hardy wein
berg equation
(P + q)2 = P2 + 2 pq + q2 (P=0.3, q = 0.7)
2 2
P = (0.3) = 0.09 = 9% AA
2pq = 2 (0.3) (0.7)= 0.42 = 42% Aa
2 2
q = (0.7) = 0.49 = 49% aa
 Hence the beetle population appears to be in Hardy Weinberg equilibrium,. Factors effecting the Hardy
Weinberg law : 1).Gene flow. 2).Mutation. 3).Crossing over. and 4). Natural selection.
21. Explain how mutations, natural selection and genetic drift affect Hardy Weinberg equilibrium.
(OR) The factors that can disturb the Hardy- Weinberg‟s equilibrium :- (May-2022)
1) No mutation – No new alleles are generated by mutation nor the genes get duplicated or deleted.
2) Random mating – Every organism gets a chance to mate and the mating is random with each other with no
preferences for a particular genotype.
3) No gene flow - Neither individuals nor their gametes enter (immigration) or exit(emigration) the population.
4) Very large population size - The population should be infinite in size.
5) No natural selection - All alleles are fit to survive and reproduce.
22. How did Darwin explain fitness of organisms?

 He noted a huge variety and remarkable similarities among organisms and their adaptive features to cope
up to their environment.
 He proved that fittest organisms can survive and leave more progenies than the unfit ones through natural
selection. For example :Industrial (in England) melanism is a classical case of Natural selection exhibited
by the peppered moth.
23. Mention the main objections to Darwinism.

1) Darwin failed to explain the mechanism of variation.
2) Darwinism explains the survival of the fittest but not the arrival of the fittest.
3) He focused on small fluctuating variations that are mostly non-heritable.
4) He did not distinguish between somatic and germinal variations.
5) He could not explain the occurrence of vestigial organs, over specialization of some organs like large
tusks in extinct mammoths, oversized antlers in the extinct Irish deer, etc.,
24. Taking the example of Peppered moth, explain the action of natural selection. What do you call the above
phenomenon?
 Industrial melanism is a classical case of Natural selection exhibited by the moth, Biston betularia.
 These were available in two colours, white and black.
 Before industrialization peppered moth both white and black coloured were common in England.
 Pre-industrialization witnessed white coloured background of the wall of the buildings hence the
white coloured moths escaped from their predators.
 Post industrialization, the tree trunks became dark due to smoke and soot let out from the industries.
 The black moths camouflaged on the dark bark of the trees and the white moths were easily identified
by their predators.
 Hence the dark coloured moth population was selected and their number increased when compared
to the white moths.
 Nature offered positive selection pressure to the black coloured moths.
 Organisms that can adapt will survive and produce more progenies resulting in increase in population
through natural selection.
25. Darwin's finches and Australian marsupials are suitable examples of adaptive radiation – Justify the statement.
1. Darwin finches :- (March -2023)
 Their common ancestor arrived on the Galapagos about 2 million
years ago.
 During that time, Darwin's finches have evolved into 14 recognized
species differing in body size, beak shape and feeding behavior.
 Changes in the size and form of the beak have enabled different
species to utilize different food resources such as insects, seeds,
nectar from cactus flowers and blood from iguanas, all driven by
Natural selection.
 Genetic variation in the ALX1 gene in the DNA of Darwin finches
is associated with variation in the beak shape.
2. Marsupials :-
 Marsupials in Australia and placental mammals in North America are separated from the common ancestor
more than 100 million year ago and each lineage continued to evolve independently.
 Despite temporal and geographical separation, marsupials in Australia and placental mammals in North
America have produced varieties of species living in similar habitats with similar ways of life.
 Their overall resemblance in shape, locomotory mode, feeding and foraging are superimposed upon different
modes of reproduction.
 This feature reflects their distinctive evolutionary relationships.
 Over 200 species of marsupials live in Australia along with many fewer species of placental mammals. Just
as the placental mammals have radiated across North America.
26. Who disproved Lamarck‟s Theory of acquired characters ? How ? (May-2022)

 Lamarck‟s “Theory of Acquired characters” was disproved by August Weismann.
 Who conducted experiments on mice for 20 generations by cutting their tails and breeding them.
All mice born were with tail.
 Weismann proved that change in the somatoplasm will not be transferred to the next generation but
changes in the germplasm will be inherited.
27. How does Mutation theory of De Vries differ from Lamarck and Darwin‟s view in the origin of new species.
 Hugo de Vries proposed by Mutation theory are “ sudden random changes that occur in an organism that is
not heritable”.
 His experiments in the Evening Primrose(O. lamarckiana)and observed variations in them due to mutation.
 According to de Vries, sudden and large variations were responsible for the origin of new species.
 Whereas Lamarck and Darwin believed in gradual accumulation of all variations as the causative factors in
the origin of new species.

28. Explain stabilizing, directional and disruptive selection with examples :-
 There are mainly three types of natural selection.
1) Stabilising Selection (centipetal selection) :-
 This type of selection operates in a stable environment.
 The organisms with average phenotypes survive whereas the extreme individuals from both the ends are
eliminated.
 There is no speciation but the phenotypic stability is maintained within the population over generation.
 Ex: measurements of sparrows that survived the storm clustered around the mean, and the sparrows that
failed to survive the storm clustered around the extremes of the variation showing stabilizing selection.
2) Directional Selection:-
 The environment which undergoes gradual change is subjected to directional selection.
 In this type removes the individuals from one end towards the other end of phenotypic distribution.
 For example, size differences between male and female sparrows. Both male and female look alike
externally but differ in body weight. Females show directional selection in relation to body weight.
3) Disruptive Selection (centrifugal selection):-
 When homogenous environment changes into heterogenous environment this type of selection is
operational.
 The organisms of both the extreme phenotypes are selected whereas individuals with average phenotype
are eliminated.
 This results in splitting of the population into sub population/species.
 This is a rare form of selection but leads to formation of two or more different species. It is also called
adaptive radiation. e.g. Darwin's finches-beak size in relation to seed size inhabiting Galapagos islands.
Stabilising Selection Directional Selection Disruptive Selection

29. Rearrange the descent in human evolution / Expalin the evolutionary path of Man ;-
Que :- Austrolopithecus → Homo erectus → Homo sapiens → Ramapithecus → Homo habilis.
Answer :- Ramapithecus → Austrolopithecus → Homo habilis→ Homo erectus→ Homo sapiens.
1) 210 million years ago-mammals evolved.
2) Hominids evolution occurred in Asia and Africa.
3) 14 mya ago - Dryopithecus – 1. Ramapithecus 2. Sivapithecus.
4) 5 mya ago – Australopithecus – Australian ape man.
5) 2 mya ago – Homo habilis.
6) 1.7 mya ago – human in looks – Homo erectus.
7) Homo ergaster and Homo erectus – first leave to Africa.
8) 34,000 – 1,00,000 years ago – Neanderthal human.
9) Cro-Magnon - ancestor of modern Europeans adapted various environmental condition cave
paintings.
10) 25000 years ago – Homo sapiens cultivating crops and domesticating animals.

30. How does Neanderthal man differ from the modern man in appearance ?
 Neanderthal human was found in Neander Valley, Germany with a brain size of 1400 cc and lived between
34,000 - 1,00,000 years ago.
 They differ from the modern human in having semierect posture, flat cranium, sloping forehead, thin
large orbits, heavy brow ridges, protruding jaws and no chin.
 They used animal hides to protect their bodies, knew the use of fire and buried their dead.
 They did not practice agriculture and animal domestication.
1. Big bang theory :-

 Theory explains origin of universe as a singular huge explosion in physical terms.
 The primitive earth consist of ammonia, methane, hydrogen and water vapour.
 UV rays from the sun split up water molecules into hydrogen and oxygen.
 When temperature cooled and the water vapour condensed to form rain.
 Ammonia, methane in the atmosphere combined with oxygen to form carbon-dioxide and other gases.
.
2. Vestigial organs :-
 Structures that are of no use to the possessor, and are not necessary for their existence are called vestigial
organs.
 These organs are included coccyx, wisdom teeth, ear muscles, body hair, mammae in male, nictitating
membrane of the eye.
3. Gene flow:- Movement of genes through gamets or movements of individuals in and out of a population is
referred to as gene flow.
4. Mutation :- Mutation is the original source of all genetic variation. Mutation rare for most organisms is low.
Hence new mutations on an allele frequencies from one generation to the next is usually not large.
5. What are coacervates? Coacervates are the first pre-cells which gradually transformed into living cells,
according to theory of chemical evolution.
6. Connecting link
 The organisms which possess the characters of two different groups are called connecting links.
 Example 1. Peripatus (Annelida and Arthropoda), 2. Archeopteryx (Reptiles and Aves).
7. What is Atavistic organs?

Sudden appearance of vestigial organs in highly evolved organisms is called atavistic organs.
E.g. Presence of tail in a human baby is an atavistic organ.
8. Lamarck‟s theory :-
 Jean Baptiste de Lamarck, was the first to postulate the theory of evolution in his famous book
„Philosophie Zoologique‟ in the year 1809. The 2 principles of Lamarckian theory are:
I. The theory of use and disuse - Organsthat are used often will increase in size and those that are not used
will degenerate. Neck in giraffe is an example of use and absence of limbs in snakes.
II. The theory of inheritance ofacquired characters - Characters that are developed during the life time of
an organism are called acquired characters and these are then inherited.

9. Modern synthetic theory :-
 Sewell Wright, Fisher, Mayer,Huxley,Dobzhansky, Simpson and Haeckel explained Natural Selection.
1) Gene mutation - refers to the changes in the structure of the gene. It is also called gene/ point mutation.
It alters the phenotype of an organism and produces variations in their offspring.
2) Chromosomal mutation - refers to the changes in the structure of chromosomes due to deletion, addition,
duplication, inversion or translocation. This too alters the phenotype of an organism and produces variations
in their offspring.
3) Genetic recombination - is due to crossing over of genes during meiosis. This brings about genetic
variations in the individuals of the same species and leads to heritable variations.
4) Natural selection - does not produce any genetic variations but once such variations occur it favours some
genetic changes while rejecting others.
5) Reproductive isolation - helps in preventing interbreeding between related organisms.
10. Darwin‟s theory of Natural Selection :-

1) Over production (or) prodigality of production - All living organisms increase their population in larger
number. For example, Salmon fish produces about 28 million eggs during breeding season
2) Struggle for existence - Organisms struggle for food, space and mate. As these become a limiting factor,
competition exists among the members of the population.
3) Universal occurrence of variations - No two individuals are alike. There are variations even in identical
twins. The useful variations struggle and such variations are passed on to the next generation.
4) Origin of species by Natural Selection - According to Darwin, nature is the most powerful selective force.
He compared origin of species by natural selection to a small isolated group. Darwin believed that the
struggle for existence resulted in the survival of the fittest. Such organisms become better adapted to the
changed environment.
-: GOD GRACE :-

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-: GOD GRACE :-

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 1

7. What is parthenogenesis? Give two examples from animals ? (May -2022)

8. Which type of reproduction is effective -Asexual or sexual and why?

10. Why is the offspring formed by asexual reproduction referred as a clone ?

11. Give reasons for the following:-

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 2

b) Budding in yeast and budding in Hydra :-

c) Regeneration in lizard and Planaria :- (March-2023)

13. How is juvenile phase different from reproductive phase? (March-2023)

14. What is the difference between syngamy and fertilization ?

1. What is paedogenetic parthenogenesis ?

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 3

4. During unfavourable conditions, how the Amoeba reproduces?

5. Difference Oviparous and Viviparous :-

6. What are the types of natural parthenogenesis? Types are :-

7. Define Parthenogenesis & Mention its types :-

8. What is Senescent phase of life ? (May -2022)

9. Plasmotomy :- (March -2023)

10. Write different types of syngamy :-

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 4

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 5

S.N Spermatogenesis Spermiogenesis

19) Expand the acronyms :-

20) How is polyspermy avoided in humans?

21) What is Colostrum? Write its significance .

22) Placenta is an endocrine Tissue – Justify ?

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 6

23) What is Inhibin ? State its functions?

24) Mention the importance of the position of testes in humans?

25) What is the composition for semen? (May-2022)

28) Define Gametogenesis ?

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 7

13. Explain the various phases of menstrual cycle ? (March -2023)

1. Menstrual phase. (3-5 days)

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 8

a) It represents ovulatory phase of Oogenesis.

b) Ovarian hormone - Progesterone, Oestrogen.

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 9

e) Write the difference between C - Secondary Follicle and H - Corpus luteum.

S.N Secondary follicle Corpus luteum

1). Mention the significance of Epididymis in the testis?

3) What are Sertoli cells and its uses ?

4) Roll of hormone in Spermatogenesis ?

5) Define : Menstrual or ovarian cycle :-

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 10

11) What is Let-Down” reflex ?

13) Discribe on extra embryonic membranes :- (May-2022)

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 11

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 12

BOOK BACK QUESTION

11) Correct the following statements :-

P.Senguttuvan. M.Sc., M.Ed.,M.Phil. & C.Valarmathi. M.Sc., M.Ed.,M.Phil. –Dharmapuri. Page 13

15) Differentiate foeticide and infanticide. :- (May-2022)

21) Open Book Assessment

4. Zygote intra-fallopian transfer (ZIFT) :-

5. Gamete intra-fallopian transfer (GIFT) :-

6. Write a short note on cervical cancer.