1 + &18

5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’

H0 -
5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ - OH : Sk 2016 (1
Ho -
3’ AAGAGACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ GH -

↓
5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ Pst =34')002
4
: 311
:

3’ AAGAGACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’

OH 5’ TTCTC 3’ 5’ TGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’
-ot
-

OH -
3’ AAGAGACGT 5’ 3’ CCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’

H0 -
5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ - OH : 12 +
&
Ho -
3’ AAGAGACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ GH -

5’ TTCTC 3’ 5’ TGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ : Pst) 1)

3’ AAGAGACGT 5’ 3’ CCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’

: 31c &2013
OH -
5’ TTCTC 3’ OH ·
HO- 5’ TGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ OH -

OH -
3’ AAGAGACGT 5’ OH -
Ho- 3’ CCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ of -

H0 -
5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ - OH : sla'd
Ho -
3’ AAGAGACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ GH -

H0 -
5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ - OH : si Gao (E
Ho -
3’ AAGAGACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ GH -

5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ OH : BamH1
Y
H0 - -

Ho -
3’ AAGAGACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ GH -

↓
H0 -

5’ TTCTCTGCAGGATATCTG 3’ 5’ GATCCACGAATTCTTTCCCGAGCTGC 3’ OH -

Ho -
3’ AAGAGACGTCCTATAGACCTAG 5’ 3’ GTGCTTAAGAAAGGGCTCGACG 5’ OH -

H0 -

5’ TTCTCTGCAGGATATCTGGATC 3’
: DNA Polymerase Klenow

Ho -
3’ AAGAGACGTCCTATAGACCTAG 5’
5’ GATCCACGAATTCTTTCCCGAGCTGC 3’ -
OH
3’ CTAGGTGCTTAAGAAAGGGCTCGACG 5’ - OH
 Ho -
5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ =
Oh :, /20016/
HOe 3’ AAGAGACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ -
Oth

5’ TTCTCTGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ : PSt
4
3’ AAGAGACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’

OH -
5’ TTCTC 3’ 5’ TGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ OH -

OH -
3’ AAGAGACGT 5’ 3’ CCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ of -

· BNA Polymerase Klenow

OH -
5’ TTCTCTGCT 3’ 5’ TGCAGGATATCTGGATCCACGAATTCTTTCCCGAGCTGC 3’ OH -

OH -
3’ AAGAGACGT 5’ 3’ ACGTCCTATAGACCTAGGTGCTTAAGAAAGGGCTCGACG 5’ of -

278105
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