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49,2440 S OLOGY MAINVIDEOS

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PICS MICROBIOLOGY MAIN VIDEOS
 GRAM POSITIVE BACILLI
DR PREETI SHARMA
MBBS, MD
GOLD MEDALLIST

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MAC DONALD Give
• Mycobacterium
• Anthracis bacillus INI
Clostridium species JEPORE
special stain
•

Corynebacterium diphtheria
•
2N stain
Nocardia FU
Schaeffer tE
•

• Actinomyces
filamentous
Ashbey stain
• Listeria MOTILITY
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• Diphtheroids

22 C 37 C
Motile nonmotile
Q) A 7-year-old child is brought to the clinic with a sore throat, low-grade
fever, and a thick grayish membrane covering the tonsils and pharynx.
The child's immunization history is up-to-date. A throat swab is collected
for culture, and Gram staining reveals Gram-positive, non-motile, non-
sporing, and non-capsulated bacilli. The isolated bacteria release a
potent toxin responsible for the characteristic symptoms. Further
investigation reveals that the toxin is encoded by a specific gene present
in some strains of the bacteria. Which of the following bacteria is most
likely responsible for this child's presentation?
a) Corynebacterium jeikeium ENDOCAR s
b) Corynebacterium diphtheriae iom
c) Corynebacterium minutissimum
MACDONALD
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d) Corynebacterium ulcerans

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 CULTURE
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Q) Which of the following bacteria is responsible for a 26-year-old male
with severe watery diarrhea, leukocytosis, and elevated creatinine after

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rural travel, culture shows Gram-negative, non-lactose fermenting
bacillus producing toxin targeting the 60s ribosome?
a) Exotoxin A of Pseudomonas aeruginosa

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b) Shiga toxin
c) Botulinum toxin ella
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d) Diphtheria toxin
surgena
Yersinia
Proteus
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Q) Interpretation ?

a) The patient has diphtheria caused by toxigenic Corynebacterium diphtheriae.

b) The patient has diphtheria caused by non-toxigenic Corynebacterium diphtheriae.
c) The patient does not have diphtheria, as no precipitin line was seen with the antitoxin.
d) The patient has a non-diphtheria bacterial infection that is producing a similar toxin.

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Q) Which of the following is the most common complication of
Diphtheria?
a) Myocarditis
b) Pneumonia
c) Meningitis
d) Endocarditis
Q) A 9-year-old boy is brought in an emergency with the complaint of
fever, throat pain, tonsillar plaques, enlarged neck lymph nodes, dyspnea
and odynophagia. As per the information given by his mother, the
immunization status of this child was inappropriate for his age. A general
physical examination revealed that he was conscious and oriented but
looked toxic. Inspiratory stridor is also present. Oropharyngeal
examination revealed greyish-white membranous patches on the medial
aspect of both tonsils. The doctor sent him for a swab test on the throat,
which confirmed diphtheria. The ideal dose of Diphtheria anti-toxin given
for the treatment is?
a) 10,000 to 1,00,000 units
b) 20,000 to 1,00,000 units
c) 10,000 to 2,00,000 units
d) 20,000 to 2,00,000 units
Type of disease Diphtheria antitoxin Antibiotics Adjunctive

Pharyngeal/ laryngeal 20,000-40,000 Penicillin or Droplet

IM or IV erythromycin precaution

Nasopharyngeal 40,000-60,000 Penicillin or Droplet

disease IM or IV erythromycin precaution
I
Extensive disease 80,000-1,20,000 Penicillin or Droplet
IM or IV erythromycin precaution

With neck disease/ Half dose IM Penicillin or Droplet

disease >3 days Half dose IV erythromycin precaution

40k
40 60k
80k 1,20140
Q) In a 50-year-old male with a nodular jaw swelling, sulfur-colored
granule discharge from sinuses, and culture-proven anaerobic gram-
positive branching rods following a tooth extraction is seen, what is the
drug of choice for treating this infection?

a) Penicillin
b) Cotrimoxazole
c) Clindamycin
d) Metronidazole
iI
S N A P
Q) In a 45-year-old male with a mandibular mass showing Splendore-
Hoeppli phenomenon, what Gram-positive, purple-colored filamentous
organism is likely responsible for the histopathological findings?

a) Staphylococcus aureus
b) Streptococcus pyogenes
c) Actinomyces species
d) Candida albicans
Q) What is the most likely causative agent for the respiratory symptoms
in a 25-year-old wool industry worker with shortness of breath, fever, and
a 'string of pearl' appearance on penicillin agar culture of the sputum
sample?
a) Staphylococcus aureus
b) Clostridium
c) Bacillus anthracis
d) Pneumococcus
Q) Which stain is best used to visualize the organism responsible for the
pustule on the back of a 42-year-old Shepherd's thigh, associated with
fever and discomfort while sitting?
a) Polychrome methylene blue
b) Calcofluor white
c) Albert stain
d) ZN stain
Q) What is the most likely causative organism for the fever, watery
diarrhea, and stomach pain in a 22-year-old male who consumed meat
and presents with capsulated, non-motile, and subterminal spore
arrangement in the microbiological test?

a) Clostridium bifermentans
b) Clostridium perfringens
c) Clostridium tetani
d) Clostridium tertium
Q) A 25-year-old female presents to the physician with complaints of
fever, chills, night sweats and productive cough for three weeks. She also
complains of weight loss for one month. The patient is otherwise vitally
stable, and other general physical examinations reveal no abnormalities.
Which of the following is true regarding the microorganism involved in
the patient's pathology?
a) Produces visible colonies in 1 week time on Lowenstein Jenson media
b) Decolorized by 10% sulphuric acid
c) Facultative aerobe
d) Growth on LJ media is characterized by rough tough and buff colonies
Q) Which type of culture media can be used for the growth of the
organism causing productive cough, fever, and weight loss in a 40-year-
old female with bilateral coarse crepitations, suspected of having
tuberculosis?
a) Tinsdale medium
b) MGIT medium
c) MYPA
d) PEMBA
MGIT → myco growth indicator tube
• Initially o2 is more and fluorescence is quenched
• When o2 decreases as MTB is an “aerobe” and will use 02 then
fluorescence increases
• Red color will blink up
Q) Which of the following is a bacteriostatic drug given in the 2 months
of intensive phase?

a)Ethambutol
b)Pyrazinamide
c) Isoniazid
d)Rifampicin