Started

Friday, November 10, 2023, 5:26 p.m.

Status : Completed

Finalized
Friday, November 10, 2023, 5:28 p.m.

Time
1 minutes 50 seconds
employee

Points 12.00/12.00

Qualification 100.00 out of 100.00

Congratulations! You have passed the Module.

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next module.
Question 1
Correct
It is scored 1.00 out of 1.00

Flag question

Question statement

The genome of a bacteria is:

to.
Exclusively the DNA that makes up your chromosome b.
The total set of DNA you have

c
c.
Exclusively the DNA that is part of its plasmids

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The correct answer is: The total set of DNA you have

Question 2

Correct
It is scored 1.00 out of 1.00
 Flag question

Question statement

Genes that confer resistance:

r
a.
They are always found on the bacterial chromosome

c
b.
They are always found in bacterial plasmids

to
c.
They can be present both on the bacterial chromosome and on plasmids.

Feedback

The correct answer is: They can be present both on the bacterial chromosome and on plasmids.

Question 3
Correct
It is scored 1.00 out of 1.00

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Question statement

Unlike Sanger sequencing, sequencing _____________________________or of _________

generation does allow the complete genome of an organism to be sequenced.

c
a.
Massive/first

to
b.
Mass/second

c
c.
Intensive / second

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The correct answer is: Massive /second

Question 4
Correct
It is scored 1.00 out of 1.00
 Flag question

Question statement

The most widely used chemistry for sequencing today is called “by synthesis”, and is based on the
generation of complementary chains to the original DNA molecules and the emission of
fluorophores, which are detected by a system:

a.
Optical

c
b.
Chemical

c
c.
Mechanic

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The correct answer is: Optical

Question 5
Correct
It is scored 1.00 out of 1.00

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Question statement

In massive sequencing of bacteria:

c
a.
A single DNA molecule is sequenced

to
b.
It is necessary to fragment the genome and subsequently reconstruct it using computer tools

c
c.
DNA extraction step is skipped
Feedback

The correct answer is: It is necessary to fragment the genome and subsequently reconstruct it using computer
tools.

Question 6
Correct
It is scored 1.00 out of 1.00
 Flag question

Question statement

This is the workflow for massive sequencing:

c
a.
Genomic library generation/sequencing/DNA extraction

c
b.
Sequencing/DNA extraction/genomic library generation
to
c.
DNA extraction / genomic library generation / sequencing

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The correct answer is: DNA extraction / genomic library generation / sequencing

Question 7
Correct
It is scored 1.00 out of 1.00

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Question statement

A genomic library is a random collection of DNA fragments to which adapter sequences have been
attached and ____________________________ (these will allow us to identify the origin of the
fragment with the sample of origin).

a.
Indices

c
b.
Indicators

c
c.
Augmenters

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The correct answer is: Indices

Question 8
Correct
It is scored 1.00 out of 1.00
 Flag question

Question statement

For each sample that we want to sequence, a genomic library is generated, however, at one point in
the process all the libraries are put together and form a ________________________. This one
must
contain all samples properly represented so the step of ________________________________is
crucial.

c
a.
Pool/sequencing

c
b.
Fragment/normalization

to
c.
Pool/normalization

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The correct answer is: Pool / normalization

Question 9
Correct
It is scored 1.00 out of 1.00

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Question statement

With the data generated by the sequencer, it is possible to obtain relevant biological information:

c
a.
Without performing any additional steps

b.
Once the required bioinformatics analyzes are performed

c
c.
Performing the analyzes in the sequencer itself

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The correct answer is: Once the required bioinformatic analyzes are performed
Question 10
Correct
It is scored 1.00 out of 1.00

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Question statement

The importance of second generation sequencing for Resistance to

Antimicrobials are that:
c
to.
It is very expensive and only allows identifying RAM genes

to
b.
It allows the pathogens to be characterized, the RAM genes they have, their genetic context and variants to be
identified. Additionally, it facilitates relevant comparisons in outbreak investigations.

c
c.
Offers results comparable to those obtained through traditional techniques

Feedback

The correct answer is: It allows you to characterize the pathogens, identify the RAM genes they have, their
genetic context and variants. Additionally, it facilitates relevant comparisons in outbreak investigations.

Question 11
Correct
It is scored 1.00 out of 1.00

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Question statement

Identification of RAM genes using WGS (whole genome sequencing):

c
to.
It guarantees that the isolate will present resistance, verifiable through antimicrobial susceptibility testing (AST).
to
b.
It is not an indicator of the level of resistance that the bacteria may have, for which antimicrobial susceptibility
tests are required.

c
c.
Will completely replace antimicrobial susceptibility testing very soon
Feedback

The correct answer is: It is not an indicator of the level of resistance that the bacteria may have, for which
antimicrobial susceptibility tests are required.

Question 12
Correct
It is scored 1.00 out of 1.00

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Question statement

Some of the limitations associated with massive sequencing are:

c
to.
Lack of personnel with training in molecular biology techniques

c
b.
Impossibility of obtaining relevant biological information even with personnel trained in bioinformatics and
sufficient computing capacity
to
c.
Lack of personnel with training in bioinformatics and limited computing capacity for both processing and
storage

Feedback

The correct answer is: Lack of personnel with training in bioinformatics and limited computing capacity for both
processing and storage.