BCDA COLLEGE OF PHARMACY &

TECHNOLOGY
NAME:SUBHADIP MONDAL
4TH YEAR,7TH SEMESTER
ROLL-20101921102
REG.NO-212010201920002 OF 2021-22
PAPER-Instrumental Methods of Analysis
TOPIC-Affinity Chromatography-Instrumentation And
Applications.
PAPER CODE-PT711
SUBMITTED TO- Dr. Debanjan Sen

CA1:POWER POINT PRESENTATION

 Affinity Chromatography
Affinity chromatography is a powerful separation
technique that allows the isolation of a protein of
interest. This presentation covers its instrumentation,
applications, and more.
Affinity chromatography is based on the specific
interaction between a target molecule and a ligand that
is immobilized on a stationary phase.

Ref.-Instrumental Methods of Analysis by Prof. Poonam, A. Salunke,

Prof. Md. Rageeb Md. Usman,Prof. Shital S. Patil,Prof. (Dr.). S. D. Barhate(page-293)
Instrumentation of Affinity Chromatography
Pump
Software
The pump is used to control the flow
rate of the buffer solution through the The software is used to control the
column. A slow and steady flow rate is instrument parameters, record the data,
important to ensure efficient separation. and analyze the protein samples.

1 2 3 4

Column Detector
The column provides a stationary phase The detector monitors the protein
where the target protein binds to the concentration and purity throughout the
ligand. Different column materials can separation process. Absorption,
be used, such as glass, plastic, or metal. refractive index, and fluorescence
detectors are common.
Ref.-Instrumental Methods of Analysis by Dr. S.MaIathi,Dr. Pallavi Mangesh Patil,Dr. Sunil Kumar (page-283)
 APPLICATIONS
1. This technique has great impact on molecular biology, biochemistry,
biotechnology.
2. Affinity chromatography is used as a powerful tool for biologically active
molecules purification process like proteins.
3. Affinity chromatography can be used whenever a suitable ligand is available for
protein of interest.
4. This technique initially used for purification of enzymes but now it is used for
various purposes like purification of nucleotides, nucleic acid, immunoglobin,
membrane receptors etc.
5.Production of Vaccines-antibody purification from blood serum.
6. To notice which biological compounds bind to a particular substance.
7. To reduce an amount of substance in a mixture.

Ref.-Instrumental Methods of Analysis by Dr. K. R. MAHADIK & Dr. L. SATHIYANARAYANAN.(page-18.4)

THANK YOU