Inventory, Constituents and

Conservation of Biologically Important

Sumatran Plants
D. Arbain, (2012), Inventory, Constituents and and Conservation of
Biologically Important Sumatran Plants, Natural Product Communication,
7(6), 799 – 806.

Dayar Arbain
Faculty of Pharmacy/Sumatran Biota Laboratory, Andalas University
Kampus Limau Manis, Padang 25163
Tel/fax : (0751)72645
E-mail: d.arbain@ffarmasi.unand.ac.id; d.arbain48@yahoo.com;

Sunset at Berbak National Park, Jambi, Sumatra 1

11/10/2012 ISNPC_Noida_2012
 Back ground

11/10/2012 ISNPC_Noida_2012 2
 In the late 70th, being a young pharmacist and a village boy who
depended on traditional medicine, there was a question kept in my mind
“Do those of Sumatran medicinal plants really have any “active”
constituents or is it merely just a “believe”.

Is there any scientific way to proof their use,

so that, it become scientifically based?
(Validation, Evicacy and Savety)

How these medicinal plants and

their related cultural values be conserved?

Being young and optimistic, I convince myself, there

should be a way to do that!

But how…???? 3

Mt. Merapi, Bukittinggi

 Sumatra is very rich with tropical rainforest plants which have been
used traditionally for food, medicine, spices, aromatics, insecticides etc.
since the beginning of civilization until 1960-th

11/10/2012 ISNPC_Noida_2012
 Background
.

Due to deforestation, the newly formed

ecosystem might not support some of
these plants to grow and develop and this
will cause the disappearance of some
of them soon.

At the same time the arts and knowledge of

how to use these plants which are kept by the
traditional healer called dukun and pawang is also
disappearing because the young generation is not
interested anymore to learn and practice this
culture, arts and knowledge.

The poor and people who live in the isolated area

stll need alernative medicine (ca 20% of population) 5

11/10/2012 ISNPC_Noida_2012
 Before these Sumatran Plants and the related
cultures of various Sumatran ethnic groups
dissapear forever, Inventory, ethnobotanical and phytochemical
survey, Biological activity and pharmacological
and conservational work
must be carried out !

Lake Maninjau

11/10/2012 ISNPC_Noida_2012
 Inventory, ethnobotanical and
phytochemical survey of
Sumatran plants
1982-2010
(more then 46 locations)

11/10/2012 ISNPC_Noida_2012 7
Inventory, ethnobotanical and
phytochemical survey of
Sumatran plants 1982-1996

1. Baso
2. Mt.Merapi
3. Mt.Sago
4. Air Sirah 10 9
* ** 1 3
5. Ulu Gadut
6. Anai 8
7* * * *11
7.
8.
Singgalang
Malampah 6* 2 * *12 * 21
9. Lurah Berangin
* *15*14
13

**416 17 *20
10. Panti 5
11. Pangkalan
12.
13.
Kelok sembilan
Sungai Dareh * * *18
19*
14. Pasir Mayang
15. Muaro Tebo
16. Mt.Kerinci
17. Mt. Tujuh
18. Mt. Keronsong
19. Sako
20. Muarobungo
8
21. Siberida
11/10/2012 ISNPC_Noida_2012
 Inventory, Ethnobotanical and Phytochemical
Survey and Biological Activity Screenings
of Sumatran Plants 1996-2008
1. Sipirok
2. Panti
3. P.Pangiraian
4. Kelok Sembilan
5. Siberida Medan
6. Surian
7. Muarolabuh
8. Gunung Tujuh
9. Danau Kerinci *1 *3 22
10. Sako
11. Danau Tes
*
*2 *4 255 *21
12. Pasir Mayang
13. Muarobungo
*
14. Muara Enim
15. Siberut * 7
6 24* 12

16. Kambang *15 *16 *8 *13 2019*

17. Seblat * *9 *
18. Arang Sepat
19. Berbak *10
17**11 *14
20. Sinami
21. Minas
22. Bukit Seligi 18 *
23. Gua Sikafir
ISNPC_Noida_2012 9
24. Simanau
25. Tesonilo
11/10/2012
 Earlier Work
1982-1987

(Chemical study of Alkaloid bearing Sumatran

medicinal plants)

11/10/2012 ISNPC_Noida_2012 10
 Early Chemical work on Sumatran Medicinal Plants (1982-1987) i.e

The Alkaloid of Sapium baccatum

H
O

Bukittinggine
D.Arbain, L.T. Byrne, J.R. Cannon, V.W. Patrick, and A.H. White, ”, Aust. J. Chem., 1990, 43, 433-7.
Panthong,A., D. Kanjanapothi, Y. Thitiponpunt, T.Taesotikul, D. Arbain, Planta Med ,1998, 64, 530-535.

The peptide alkaloids from

Antidesma montana

• Dayar Arbain and W.C. Taylor, Phytochemistry,

Dayar Phytochemistry, 1993, 33, 1263
• Arbain, T. Langley, K. Pitcher, and W.C. Taylor, Aust.J.
Dayar Arbain, Aust.J. Chem., 1991, 44, 887-
887-90
11/10/2012 ISNPC_Noida_2012 11
 The Alkaloids from Margaritaria indica
O O O

R1 H R1 H H
R2 R2
N N N
OH
1. Phyllochrysine, R1=R2=H 3. Securinine, R1=R2=H
2. Securinine, R1=H, R2=OMe 4. (4a)-4-methoxysecurinan-11-one 6. securinol A
(4-epiphyllanthione, R1=H, R2=OMe)
5. (4b)-4-methoxysecurinan-11-one
(phyllanthione, R1=OMe, R2=H)

O O

H H

N N
NH
OMe N
H

7. 14,15-dihydro-15a- 8. margaritarine
methoxyphyllochrysine

Arbain,D., et al, Aust. J. Chem., 1990, 43, 439-

439-45.
1863-9.
Arbain,D., et al, J. Chem. Soc. Perkin Trans. I, 1991, 1863-
11/10/2012 ISNPC_Noida_2012 12
 The constiuents of Picrasma javanica

D. Arbain and M.V. Sargent, Aust. J. Chem., 1987, 40, 1527-36.

Dayar Arbain, et al., Aust. J. Chem., 1990, 43, 433-7.

(unpublished work)
13

11/10/2012 ISNPC_Noida_2012
 Chemical study on Sumatran
Rubiaceaus Plants
(1987-Now)

Biological activity based:

(Hyppocratic, Antimicrobial
and
Antioxidant Screenings)

11/10/2012 ISNPC_Noida_2012 14
 Why Rubiaceus Plants?

• Taxonomically advanced
(more complex biosynthesis,
more complex molecules)
• Economically valuable
(Coffea, Cinchona, Uncaria)
• Medicinal
(Cinchona, Ipecac, Gambir)
•Scientifically
Scientifically less explored
•Some
Some are rare and hard to collect

11/10/2012 ISNPC_Noida_2012 15
 Chemical Study on Sumatran Rubiaceus Plants
Chemical study on
Sumatran Ophiorrhiza
1.Ophiorhiza filistipula
2. O.major
3. O. ferruginea
4. O.cf.ferruginea
5. O.marginata
6. O.discolor
7. O.communis
8. O.cf.communis
9. O.bracteata
10. O.cf.bracteata
11. O. rosacea
12. O. cf. rosacea
13. O. kunstleri
14. O. cf. kunstleri
15.Ophiorrhiza 7562
16.Ophiorrhiza 6526A
17.Ophiorrhiza 6526B
18.Ophiorrhiza 6604
19.Ophiorrhiza 7931
20. Ophiorrhiza sp (ex Sgl)
21.Ophiorrhiza sp (exSJ 11/10/2012
Chemical study on
other Rubiaceus
Sumatran Plants
22.Cephaelis stipulacea
23.Uncaria glabrata
24.Uncaria cf.glabrata
25.U. jasminiflora
26.Lerchea bracteata
27. L.parvifolia
28. Lerchea sp (ex Kbg)
29. Lerchea sp (ex Bgs)
30. Lerchea sp (ex Pr)
31. Hedyotis auricularia
Garaman kancia 32 .H. verticillata
33. H.philippensis
(Ophiorrhiza sp) 34. Anthocephalus cadamba
ISNPC_Noida_2012 16
 The Alkaloids of Sumatran
Ophiorrhizas

11/10/2012 ISNPC_Noida_2012 17
 A New Quaternary Indole Alkaloid
Related to Cinchonamine from
Ophiorrhiza major

11/10/2012 ISNPC_Noida_2012 18
 The alkaloids of Ophiorrhiza filistipula

19
Ohba, M. H. Kubo, T. Fujii, H. Ishibasi, , M.V. Sargent and D. Arbain, Tetrahedron Lett., 1997, 38(38), 6697- 6700.
ISNPC_Noida_2012
11/10/2012
 The Alkaloids of Ophiorhiza cf.ferruginea

Arbain,D.,
11/10/2012 D.P.Putra, N.H.Lajis and M.V.Sargent, et al., Aust. J. Chem., 1993, 46, 969-
969-976. 20

ISNPC_Noida_2012
 The alkaloids from Ophiorrhiza bracteata

11/10/2012 ISNPC_Noida_2012 21

Arbain,D., L.T. Byrne, Dachriyanus, N. Evrayoza and M.V. Sargent, Aust .J .Chem.1997, 50,1111-
50,1111-1112
The alkaloids of Ophiorrhiza blumeana

11/10/2012 ISNPC_Noida_2012 22

D.Arbain, et al., J.Chem.Soc.Perkin Trans I, 1998, 2537-40

Research Results 09
The Isolation and Identification of
Major Alkaloid of Ophiorrhiza sp
(ex Sei Janiah) (Rubiaceae)

N
N
H CH3
Harman

Arbain, Annisa, Putra, 1012

Previously isolated from O.communis, O. tomentosa and Ophiorrhiza Sp

(Arbain et al, ACGC Chem. Res. Commun., 1998,7, 44-47 and
Hamzah et al, Pertanika J. Sci. and Technol., 1994, 2(1), 33.)

23
11/10/2012 ISNPC_Noida_2012
 A Study of Sumatran Uncaria

Sumatra produces 90 % of world gambier

(extracted from Uncaria gambier)

11/10/2012 ISNPC_Noida_2012 24
 The Alkaloids of Uncaria glabrata DC

Arbain,D., et al, J. Chem. Soc. Perkin Trans. I, 1992, 665-

665-6.
Arbain,D.,et al., Aust. J. Chem., 1993, 46, 863 25

ISNPC_Noida_2012
11/10/2012
 The Alkaloids from Uncaria cf. glabrata DC

26
Dayar Arbain, Afrida, S. Ibrahim and M.V. Sargent Aust. J. Chem., 1998, 51, 961-
961-964.
ISNPC_Noida_2012
11/10/2012
 A Study of Sumatran Lerchea

There are 19 species of this genus so far

reported exist in the world and only 8 of
them found in Indonesia, 2 species in Java
and 6 species in Sumatra.

(Kew Plant Database (Accessed October 2006).

11/10/2012 ISNPC_Noida_2012 27
 The Quaternary Alkaloid of
Lerchea bracteata Val.

Lercheine

Dayar Arbain, D.P. Putra, N.H. Lajis, M.V. Sargent, and A.H. White, A New
Quaternary Alkaloid from Lerchea bracteata, J. Chem. Soc. Perkin Trans. I,
1992, 3039.

11/10/2012 ISNPC_Noida_2012 28
 Antibacterial screening of some of Sumatran Lerchea sp
No Lerchea spp Inhibition zone (mm) for 5 mg/disk
Bs Ec Pa Kl Sa Ml Tm Ca

1 Lerchea interrupta Ext. MeOH 10.5 7.5 9.5 9.5 6.0 8.5 - -
Ext. Hexane 11.0 9.5 10.5 10.0 10.5 12.5 - -
Ext. EtOAc 6.5 7.5 7.5 7.0 7.5 8.0 - -
Ext. BuOH 9.5 7.5 10.0 11.5 11.5 9.5 - -
2 Lerchea parviflora Ext. MeOH 6.0 - 6.0 7.5 6.5 12.0 - -
Ext. Hexane 5.0 - 5.0 14.5 6.0 15.5 - -
Ext. EtOAc 7.5 - 10.5 7.5 9.0 7.5 - -
Ext. BuOH 8.5 - 8.5 14.0 15.5 11.5 - -
3 Lerchea beccariana Ext. MeOH 7.5 - 6.5 23.0 6.0 - - -
Ext. Hexane 6.0 9.5 8.0 - 8.5 6.0 - -
Ext. EtOAc 9.5 7.5 10.0 7.0 7.5 6.0 - -
Ext. BuOH 13.5 11.5 12.5 26.0 11.5 7.5 - -
4 Lerchea cf. bracteata Ext. MeOH 6.5 - 8.5 - 9.5 - - -
Ext. Hexane 6.0 - 6.5 6.0 7.5 6.5 - -
Ext. EtOAc 8.5 9.5 10.5 9.5 11.5 8.5 - -
Ext. BuOH 6.0 - 7.5 - 6.5 - - -
5 Lerchea sp. Ext. MeOH 7.5 6.5 8.5 6.0 10.5 7.5 - -
Ext. Hexane 5.5 5.0 8.0 - 6.0 7.0 - -
Ext. EtOAc 6.0 5.0 8.0 5.0 8.5 6.5 - -
Ext. BuOH 6.5 7.5 7.5 6.0 9.0 - - -
Bs=Bacillus subtilis, Ec=Escherichia coli, Pa=Pseudomobnas aeruginosa, Kl=Klebsiella sp, Sa=Staphylococcus aureus, Ml=Micrococcus luteus, 29
Tm=Trycchophyton mentagrophytes, Ca-Candida albicans
11/10/2012
ISNPC_Noida_2012
 The Isolation and Identification
of Major Alkaloid of Lerchea cf.bracteata
(ex Kambang) (Rubiaceae)
(strong antibacterial activities)

Antirhine metochloride
(Previously isolated from Hunteria eburnea Pichon)
Burnell, R.H., Chappele, A. and Khalil, M.F., Can. J. Chem,
1974, 52, 2327-2330)

Dayar Arbain, N. Syafni, Friardi, D. P. Putra, I. Komala, K. Yamaguchi and Y. Asakawa,

The Quaternary Indole Alkaloids from Two Sumatran Lerchea species, Natural Product
Communications, 2011,6 (3), 349-352.
30

11/10/2012 ISNPC_Noida_2012
11/10/2012 ISNPC_Noida_2012 31
 COSY

HMBC HSQC

11/10/2012 ISNPC_Noida_2012 32
Research Results 09 The Isolation and Identification
of Major Alkaloid of Lerchea partviflora
(ex Bungus) (Rubiaceae)
(strong antibacterial activities)

6,7-dihydroflavopereirine
(reported to be active against Plasmodium)

Peviously reported by Angenot (Planta Med, 1973, 23, 226-32)

D.Arbain, N. Syafni, Friardi, D. P. Putra, I. Komala, K. Yamaguchi and Y. Asakawa, The Quaternary Indole
Alkaloids from Two Sumatran Lerchea species, Natural Product Communications, 2011,6 (3), 349-352.
33

09/10/2012 HKBAI_UIN_2012
 Leeaceae Barisan range of Sumatra
Apocynaceae Ternstromiaceae
1.Voacanga foetida 15.Leea indica
2.Rauvolfia sumatrana
13.Eurya acuminata 16.Leea aculeata
3.Dyera costulata
Verbenaceae Piperaceae
Rutaceae 14.Clerodendron siphonanthus 19.Piper mollissimum
4.Evodia cf. trichotoma 20. Piper magnibacum
5.Elatostema sinuata Menispermaceae 21. Piper porphyrophyllum
6.E. acuminata 18.Stephania hernandifolia
7.Glycosmis sapindoides Simarubaceae
22.Picrasma javanica
Moraceae
23. Brucea sumatrana
Lauraceae 17.Ficus deltoideus
8.Phoebe lanceota Euphorbiaceae
9.Litsea elliptica 24.Margaritaria indica
25.Sapium baccatum
Rhizophoraceae 26.Antidesma montana
10.Pellacalyx axillaris Chemical 27.A.tetrandra
28.Macaranga javanica
Illiciaceae
study on other
Myrtaceae
11.Illicium anisatum Medicinal Sumatran 29. Syzigium polianthum

Celastraceae plants 30. Calophyllum enervosum

12.Cestrum nocturnum 31. Boea treubii

(D. Arbain, (2012), Inventory, Constituents and conservation of Biologically Important

34
Sumatran Plants, Natural Product Communications, 7 (6), 799-806. 34
11/10/2012
ISNPC_Noida_2012
 Chemical study of some
“the neglected”
Sumatran lower plants

11/10/2012 ISNPC_Noida_2012 35
11/10/2012 36

ISNPC_Noida_2012
Some collecting trips have been carried out in various parts
of West Sumatran Forests during 2008-2009 to collect
various kind of fungy, lichens, liverworts and ferns,
So far, some lower plants below are being studied
for their chemical constituents

1. Gleichenia linearis (Burm.) Clar

2. Scleroderma sinnamariense Mont
3. Bazzania sp
4. Stereocoulon sp
5. Dumortiera sp
6. Plagiochylla sp
7.Trichomanes sp
8. Diplazium esculentum
9. Asplenium nidus
9. Hymenophyllum sp

(main problem is how to identify them scientifically)

11/10/2012 ISNPC_Noida_2012 37
 Gleichenia linearis (Burm.) Clarke
Unwanted weed,
OH
destroys the fertile soil
and prevent other plants from NaO3SO O

growing around this plant OH

OH O
Na Kaempferol Sulfate

OH

NaO3SO O

O HO
OH O
O OH
Na kaempferol 7-sulfate-
3-glucopyranoside
CH2OH

Jubahar J., DachrIyanus, Arbain D., Bakhtiar A., Mukhtar MH., Sargent MV, A Flavonoid Sulfate from
Gleichenia
11/10/2012 linearis (Burm; Clarke), ACGC Chem.Res. Commun. 20: 6-7, 2006 38

ISNPC_Noida_2012
 Research Results 2009

Isolation of the major constituents of fungus

Scleroderma sinnamariense Mont.
(Sclerodermataceae)
(The common mushrom of Gnetum gnemon,
Its methanolic extract strongly inhibited the growth of some plant pathogenic bacteria)

O
MeO
O
OMe

OR
MeO O

I. R = H

II. R = Me

D. P. Putra, Nurmilasari,I. Komala, Y. Asakawa and Dayar Arbain, The Coloring

Constituents of Scleroderma sinnamariense (Sclerodermaceae), Natural Product
Communications, 2011, 6 (3), 357-360

11/10/2012 ISNPC_Noida_2012 39
Scleroderma sinnamariense
Inhibition of 4’,4”- dimethoxypulvinic acid (II)
toward some phytopathogenic bacteria

Pytophatogenic Host plant MIC (ppm)

bacteria

Ralstonia solanacearum Musa sp. 100

Pseudomonas solanacearum Solanum 3.1

lycopersicum L.
Pseudomonas stewartii Zea mays L. 3.1

Xanthomonas axonopodis Allium ascalonicum L. 200

pv.allii
Clavibacter michiganensis pv Solanum 100
michiganensis lycopersicum L.
11/10/2012 ISNPC_Noida_2012 40
Scleroderma sinnamariense

Antimicrobial Acvtivity test of

Scleroderma sinnamariense Mont MeOH extract

Concentration (mg/disk)
Microbes 2 1 0.5 0.25 C (0.03)

diameter of inhibition (cm)

Micrococcus luteus 0.9 0.8 0.7 0.7 2.5

Staphylococcus aureus 0.9 0.8 - - 1.1
Bacillus cereus 1.0 0.9 0.8 - 1.2
Pseudomonas aeruginosa 0.9 0.7 - - 1.2
Salmonella parathypii 0.9 0.8 - - 1.1
S.epidermidis 1.0 0.9 0.9 - 1.2
Escherichia coli - - - - 1.3
11/10/2012 ISNPC_Noida_2012 41

C = standard chloramphenicol
 Antioxidative activity of extract and fractions of
S. sinnamariense (DPPH Methode)
No Time MeOH Hexane EtOAc BuOH T

(Min) (%) (%) (%) (%) (%)

1 1 30.57 86.75 94.52 38.51 44.2

2 3 33.75 87.10 94.52 42.40 65.8
3 5 36.04 87.28 94.52 44.87 71.6
4 10 38.86 87.63 94.52 48.59 75.2
5 15 41.70 87.98 94.35 51.24 75.2
6 20 44.52 89.40 94.35 54.06 75.6
7 25 46.64 88.69 94.35 56.36 75.6
8 30 48.76 88.86 94.35 58.48 75.7
9 35 50.53 89.05 94.35 60.07 75.7
42
T = Trolox
11/10/2012 ISNPC_Noida_2012
Research Results 08
The Isolation and Identification
of the constituents of
Lichen Stereocoulon philippinense

1st isolation attemp

New compound, not Nat Prod but an artefact
and 2 (two) more isolated compounds

2nd isolation attemp

3 compounds isolated
1 identified as atranorin
2 decomposed within 3 days Atranorin

(Konig & Wright, 1999. Phytochem. Anal., 10, 279-284)

11/10/2012 43

ISNPC_Noida_2012
11/10/2012 ISNPC_Noida_2012 44
 Antimicrobial activity of MeOH extract of some Sumatran Ferns

*) µg/disk ISNPC_Noida_2012 45
11/10/2012
11/10/2012 ISNPC_Noida_2012 46
11/10/2012 ISNPC_Noida_2012 47
 Chemical Study of Trichomanes sp
-Traditionally used to treat diarhea and skin infections
- Found significantly active to inhibit the growth of
Escherichia coli,
Staphylococcus aureus ,
Staphylococcus epidermidis
Vibrio cholera
Salmonella typhimurium
- Three compounds isolated;
3,4-dihydroxybenzoic acid (I)
3,4-dihydroxybenzaldehyd (II)
3-hydroxybenzaldehyd-4β-O-D-glucopyranoside

CHO
H 1 H
6 2
5 3
4
H OH
OH

48
11/10/2012 ISNPC_Noida_2012
 DPPH-ANTIOKSIDANT TEST
of extract and fractions of Trichomanes sp.

Concentration 5 mg/5 mL (MeOH)

Samples 200 µL 100 µL 50 µL 25 µL
% Inhibition:
Stem MeOH Ext. 90,14 79,08 59, 13 47,67
Hexane Frc 18,19 13,86 9,61 9,37
EtOAc Frc 90,38 91,66 92,78 92,95

Leaves MeOH Ext 82,45 68,67 59,93 51,60

Hexane Frc 48,24 33,89 27,32 21,955
EtOAc Frc 91,58 90,06 90,06 87,41

EtOAc Fraction Concentration 1 mg/5 mL (MeOH)

200 µL 100 µL 50 µL 25 µL
% Inhibition:
Stem 65,07 45,61 24,71 10,87
Leaves
11/10/2012 87,50 ISNPC_Noida_2012
71,94 42,94 22,92 49
 1H NMR of 3,4-Dihydroxybenzoic Acid

CO2H
J3-5=2.5 Hz
H 1 H
6 2
J5-6=8.5 Hz
5 3
4
H OH
OH
J3-6=1Hz

11/10/2012 ISNPC_Noida_2012 50
 HMBC

COSY
HSQC

11/10/2012 ISNPC_Noida_2012 51
 Inihibition test of extract and fractions of Trichomanes sp
toward the growth of Escherichia coli

MeOH Extract

10% 20%

10%
Heksane
10% Fraction
10% & 20%
Water
20% 20%
Fraction
10%

20% 10%

EtOAc Fraction

11/10/2012 ISNPC_Noida_2012 52
 On going Chemical of Some Sumatran Fern

Hymenophyllum javanicum
Drymoglossum piloselloides
Asplenium nidus

So far yielded stigmast-5-en-3β-ol

11/10/2012 ISNPC_Noida_2012 53
 Some spectra of stigmast-5-en-3β-ol

HO
stigmast-5-en-3ß-ol

11/10/2012 ISNPC_Noida_2012 54
 On going work on Sumatran Ferns

11/10/2012 ISNPC_Noida_2012 55
 Conservation of Useful and
Endangered Sumatran Plants

(2001-2010)

11/10/2012 ISNPC_Noida_2012 56
 Special Grant
2000 – Vice President RI-
2003 - President RI-
Bank Mandiri Special Grant
2004. Dept. National Education
2006. BPOM
Ex situ Conservation
of Sumatran Plants

Botanical Garden
of Andalas University :
(154 ha)

1.Medicinal Plant Garden

(KTOF, already opened and
planted 12 ha)

2.Arboretum
(ARB, already opened 12 ha)

3.Biological Research
11/10/2012 ISNPC_Noida_2012 57
Forest (HPPB, primary forest)
 11/10/2012 ISNPC_Noida_2012 58

Preparing the land for planting seedling collected from various location of Sumatran forests
 three years ago

11/10/2012 ISNPC_Noida_2012 59
 Conclusions
•More than 150 Sumatran plants have been investigated mainly for their main
major chemical constituents and preliminary biological activities

•Most of Sumatran Traditional Medicinal plants investigated

so far contained active or closely related to active compounds reported in Literature.

•More work has to be done on their biological activities and innovative and applied
research work still needed.

•Some Sumatran medicinal plants are very difficult to find now.

Need special attention to conserve these useful plants together with traditional culture,
arts and knowledge related to them.

Some of my dream in late 1970-th has come true and stll a lot more to be done

11/10/2012 ISNPC_Noida_2012 60
Sumatran Biota Laboratory
Andalas University Acknowledgment
(for Recent Study on Sumatran Plants

Co-workers’
1. Prof. Dr. Amri Bachtiar
2. Prof .Dr. Deddi P. Putra
3. Dr. Friardi, Apt
4. Nova Syafni,
4. Sonya Annisa
6. Madonna Yennita
1. The OC for invitation and help 7. Vitrillia Rachmawary

2. The Competency Grant of Miistry of Education RI

(2008-2010) is gratefully acknowledged

3. We thank Professor Y. Asakawa from Faculty of Pharmacy,

Tokushima Bunry University for providing the NMR and
MS spectra as well as some key references.
61

11/10/2012 ISNPC_Noida_2012
30 September 2009
earthquake

11/10/2012 ISNPC_Noida_2012 62
 11/10/2012 ISNPC_Noida_2012 63

Presentation at Presidential Palace, 28 Februari 2003 (recognition usually comes late?)