Catalase test

Oxidase test
Coagulase test
Haemolysis on Blood agar
 Bile Solubility test
sodium deoxycholate

Streptococcus pneumoniae
 Sugar Fermentation test

pH indicator –
Phenol red

Durham’s tube - Gas

 Oxidation- Fermentation test
OF medium or Huge & Leifson’s medium

Tryptone, sugars & Bromothymol blue

CAMP test
 Indole test
Tryptophane  Indole

Kovac’s reagent
 Methyl Red test

Pyruvate to mixed acid fermentation

Methyl Red indicator

 Voges-Proskauer

2,3 Butanediol pathway

Production of acetoin from pyruvate identified in the

presence of 40% KOH and alpha naphthol
 Citrate Utilization test

Simmon’s Citrate agar

Na citrate  Na & Citrate  Na + CO2  NaCO2

Bromothymol blue
 Urease test
Urea  ammonia products  increase pH

Phenolphthalein indicator

Proteus spp.
 Triple Sugar Iron Agar test
0.1% glucose, 1% lactose, 1% sucrose
Phenol red – Red @ pH 8.2, yellow @ pH 6.4
Black ppt of ferrous sulphide – H2S production

E. coli, Enterobacter, Kelbsiella, Yersinia enterocolitica, Proteus

vulgaris – acid (yellow) butt, acid (yellow) slant

Salmonella, Proteus mirabilis, Yersinia pseudotuberculosis, Y. pestis

– acid (yellow) butt, alkali (red) slant

Salmonella, Proteus – H2S production