CENTRO ESCOLAR UNIVERSITY

SCHOOL OF MEDICAL TECHNOLOGY

MOLECULAR BIOLOGY
LABORATORY

NAME: ____________________________ DATE: ______________

YR/SECTION: ______________________ GROUP: ____________
ACTIVITY # 1
SAFETY PRACTICES IN THE LABORATORY
LEARNING OBJECTIVES
At the end of this activity the learner should be able to:
1.1 Enumerate the Universal Precautions in the Laboratory
1.2 Identify the risks in the bacteriology section posed by bio-hazardous materials & be
able to
identify the different biosafety & toxic hazard symbols.
1.3 Define nosocomial infection & give examples
1.4 Know the general concepts for specimen collection and handling.
1.5 Demonstrate correct hand washing technique.
1.6 Know the proper use of a fire extinguisher.

PRE LAB-DISCUSSIONS

COLOR CODING IN WASTE DISPOSAL

INFECTIOUS/ PATHOLOGICAL
SHARPS
DRY – NON INFECTIOUS
WET – NON INFECTIOUS
CHEMICALS
RADIOACTIVES
 CENTRO ESCOLAR UNIVERSITY
SCHOOL OF MEDICAL TECHNOLOGY
MOLECULAR BIOLOGY
LABORATORY

LAB SAFETY SYMBOLS

____________________________

_____________________________

_____________________________

_____________________________

_____________________________

_____________________________

_____________________________
 CENTRO ESCOLAR UNIVERSITY
SCHOOL OF MEDICAL TECHNOLOGY
MOLECULAR BIOLOGY
LABORATORY

HOW TO USE A FIRE EXTINGUISHER

P - ________________________________
A - ________________________________
S - ________________________________
S - ________________________________

PROPER HANDWASHING TECHNIQUES

 CENTRO ESCOLAR UNIVERSITY
SCHOOL OF MEDICAL TECHNOLOGY
MOLECULAR BIOLOGY
LABORATORY

DONNING AND DOFFING OF GLOVES

 CENTRO ESCOLAR UNIVERSITY
SCHOOL OF MEDICAL TECHNOLOGY
MOLECULAR BIOLOGY
LABORATORY

ACTIVITY

A. DEMONSTRATE THE PROPER PROCEDURE ON HANDWASHING

RUBRICS 1 0
1. Student removes jewelry and accessories on the
hands and wrist.
2. Student has prepared all necessary materials
needed for the procedure.
3. Student turns on the faucet and allows water to flow
into the sink, wets hands with water.
4. Student applies soap on his/her hands, rubs hands
together to form a lather.
5. Student rubs hands palm to palm.
6. Student rubs back of each hand with palm of other
hand with fingers interlaced.
7. Student rubs palm to palm with fingers interlaced.
8. Student rubs with back of fingers to opposing palms
with fingers interlocked.
9. Student rubs each thumb clasped in opposite hand
using a rotational movement.
10. Student rubs tip of fingers in opposite palms in a
circular motion.
11. Student rubs each wrist with opposite hand.
12. Student with fingers facing down, rinse hands with
water.
13. Student dries hands thoroughly using towel / paper
tissue.
14. Student switches faucet off using the used towel /
paper towel.
15. Student disposes wastes generated in appropriate
containers.
 CENTRO ESCOLAR UNIVERSITY
SCHOOL OF MEDICAL TECHNOLOGY
MOLECULAR BIOLOGY
LABORATORY

B. DEMONSTRATE THE PROPER PROCEDURE OF REMOVING GLOVES

RUBRICS 2 0
1. Pinch and Hold the outside of the gloves near the
wrist area
2. Peel downward and away from the wrist turning the
glove inside and out
3. Pull the glove away until it is removed from the
hand, holding the inside out glove with the gloved
hand
4. With your un gloved hand, slide your finger under the
wrist area of the remaining glove and pull downwards
5. Continue to pull downward until the gloves are
removed. Dispose gloves and wash hands

POST LAB QUESTIONS

1. DIFFERENTIATE RISK GROUPS FROM BIOSAFETY LEVELS (10PTS)
______________________________________________________________________
______________________________________________________________________
______________________________________________________________________

PREPARED BY:

PETER V. SAMONTE,RMT, MSc, MLS(ASCPi)CM

REFERENCES
1. A Guide to Biosafety & Biological Safety Cabinets. Esco Group of Companies.
Accessed March 2021. http://www.escoglobal.com/resources/guide-to-biosafety-and-
biological-safety-cabinets/13/.
2. Biosafety in Microbiological & Biomedical Laboratories (BMBL). (2020). Sixth Edition.
U.S. Department of Health & Human Services. Public Health Service. Centers for
 CENTRO ESCOLAR UNIVERSITY
SCHOOL OF MEDICAL TECHNOLOGY
MOLECULAR BIOLOGY
LABORATORY

Disease Control & Prevention. National Institutes of Health. Accessed March 2021.
https://www.cdc.gov/labs/BMBL.html.
3. Buckingham, L., Flaws, M.L. Molecular Diagnostics: Fundamentals, Methods, &
Clinical Applications. (2007). F.A. Davis Company. Philadelphia.
4. Canadian Biosafety Standards & Guidelines for Facilities Handling or Storing Human
& Terrestrial Animal Pathogens, & Toxins. (2015). Second Edition. Public Health Agency
of Canada. Accessed March 2021. https://www.canada.ca/content/dam/phac-
aspc/migration/cbsg-nldcb/cbs-ncb/assets/pdf/cbsg-nldcb-eng.pdf
5. Laboratory Biosafety Manual & Associated Monographs. (2020). Fourth Edition.
Geneva: World Health Organization. Accessed March 2021.
https://www.who.int/publications/i/item/9789240011311.
6. Principles & Concepts of Biosafety. (2020). University of Missouri: Environmental
Health & Safety. Accessed March 2021. https://ehs.missouri.edu/bio/principles.
7. Ranger, B. & Hamilton, L. The Biosafety Program. (2008). The University of
Tennessee Knoxville: Environmental Health & Safety. Accessed March 2021.
https://biosafety.utk.edu/the-biosafety-program/.