SOP for Microscope

Standard operating procedure to observe the micro-organisms and crystal shape with photograph of crystalline product of insulin by microscope.
1.0 Objective
To clearly define the operation of the Microscope.
2.0 Scope
This procedure is applicable to observe the micro-organisms and crystal shape with photograph of crystalline product of insulin.
3.0 Responsibility
3.1 Doing: Technical Assistant/Executive
3.2 Checking: Executive /Manager

4.0 Accountability
Head of the Department
5.0 Procedure
5.1 For Microscope
5.1.1 Turn “ON” the microscope.
5.1.2 Set the “Rheostat control knob” to the lowest setting.
5.1.3 Fully open the field diaphragm by turning the control level clockwise. Also fully open the aperture diaphragm of the condenser by moving the
level to the extreme right.
5.1.4 Using “Sub stage condenser focusing knob”, raise the condenser to the top position.
5.1.5 Place the specimen slide on the stage.
5.1.6 Rotate the nosepiece to move the 10 X objective into working position.
5.1.7 Lower the 10 X objective by rotating the ‘coarse adjustment knob’ to its positive stop.
5.1.8 Adjust the condenser in sharp focus using the “Condenser focusing knob”.

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5.1.9 Sharpen the focus using “fine adjustment knob”.

5.1.10 Adjust proper field & high power 40 X objective or 100 X oil emulsion objective, by rotating the “coarse adjustment knob”.

5.2 Photography
5.2.1 After adjusting the proper field of the specimen in sharp position, adjust the attachment of camera on the computer.
5.2.2 Open the directory ATI multimedia.
5.2.3 Open the shutter for getting image on the computer.
5.2.4 Then click on the button marked “Auto scan”, “OK”.
5.2.5 Click the tuner mode, for the screen.
5.2.6 Click VHS connector.
5.2.7 Click capture still for taking photograph after setting proper field by fine adjustment of the microscope.
5.2.8 Click the print image, for getting the printout.
5.2.9 After completion of work, close the ATI player and switch ‘OFF’ the microscope.
6.0 Abbreviation
Nil
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