TECHNICAL NOTE

Optical Raw datas description TN/2010/06/003

Mythic 22 OT and Mythic 22 CT

Made by: Michel Legrand – Orphée S.A.

Validated by: Jacek Stavicki – Orphée S.A.

To diffuse to: All service engineers

DIF: Total number of impacts read by the bench (WBC, µbubbles, cellular wastes, ghosts ...)

Mat : Total number of impacts corresponding to WBC (Lym, Mono, neutro, eosino, baso)

LYM : number of impacts corresponding to LYMPHOCITES.

MON : number of impacts corresponding to MONOCYTE.

NEU : number of impacts corresponding to NEUTROPHIL.

EOS : number of impacts corresponding to EOSINOPHIL.

BAS : number of impacts corresponding to BASOPHIL.

IC : number of impacts corresponding to immature cells.

N1 :number of impacts corresponding to N1.

 TECHNICAL NOTE

Optical Raw datas description TN/2010/06/003

Mythic 22 OT and Mythic 22 CT

Made by: Michel Legrand – Orphée S.A.

Validated by: Jacek Stavicki – Orphée S.A.

To diffuse to: All service engineers

N2 : number of impacts corresponding to N2.

ALL H : number of impacts over the level high ALL H (saturation high on ALL).

ALL L : number of impacts over the level low ALL H (saturation low on ALL).

NH L : number of impacts in the zone (see the graph below)

NH H : number of impacts in the zone (see the graph below)

NL L : number of impacts in the zone (see the graph below)

NL H : number of impacts in the zone (see the graph below)

RL L : number of impacts in the zone (see the graph below)

RL R : number of impacts in the zone (see the graph below)

WBC : Number of WBC counting by the resistive (counting heads)

O-CL : Ratio betwen the Mat value and the WBC value multiply by 100 [(Mat/WBC)*100]

If O-CL < 30 mean OPT – alarm appears after sample reading

>>Mean not enough impacts on the scat gram regarding the resistive reading

If O-CL < 10 mean O-CL alarm appears after sample reading

>> Mean injector partially or totally clogged.
 TECHNICAL NOTE

Optical Raw datas description TN/2010/06/003

Mythic 22 OT and Mythic 22 CT

Made by: Michel Legrand – Orphée S.A.

Validated by: Jacek Stavicki – Orphée S.A.

To diffuse to: All service engineers

 TECHNICAL NOTE

Optical Raw datas description TN/2010/06/003

Mythic 22 OT and Mythic 22 CT

Made by: Michel Legrand – Orphée S.A.

Validated by: Jacek Stavicki – Orphée S.A.

To diffuse to: All service engineers

 Scattergram flags
FLAG MEANS SUSPICION % VALUE # VALUE
NH N1 Noise 1 Presence of platelet Percentage of cells in the zone Number of cells in
aggregate, debris or in regard of the total number the zone.
resistant RBC of leucocytes in the
scattergram.
N2 Noise 2 Presence of platelet Percentage of cells in the zone Number of cells in
aggregate, erythroblast or in regard of the number of the zone.
N1 small lymphocytes. lymphocytes.
IC Immature Presence of Immature Percentage of cells in the zone Number of cells in
NL in regard of the total number the zone.
Cells Cells (from the mono
HL or of leucocytes in the
IC polynucleated cells line) scattergram.
RL Right Presence of atypical Percentage of cells in the zone Number of cells in
Lymphocytes lymphocytes or in regard of the number of the zone.
basophiles. lymphocytes and monocytes.
RL
N2 HL High Presence of basophiles, Percentage of cells in the zone Number of cells in
Lymphocytes small neutrophils in regard of the number of the zone.
(without granulations lymphocytes and neutrophils.
or few
segmented), band cells.
NL Neutrophils Presence of small Percentage of cells in the zone Number of cells in
Low neutrophils (without in regard of the number of the zone.
granulations or few monocytes and neutrophils.
segmented), band cells or
hyper basophil monocytes.
NH Neutrophils Presence of giant Percentage of cells in the zone Number of cells in
High neutrophils, hyper in regard of the number of the zone.
segmented neutrophils, eosinophils and neutrophils.
eosinophils with few
granulations or damaged
eosinophils.
Flags
• NH, NL, RL, HL:
– % and # calculated from
the two concerned
populations.
• N1, N2, IC:
– % and # calculated from
the total scattergram