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On Site Urine Treatment Combining Calcium Hydroxide Dissolution and Dehydration With Ambient Air
On Site Urine Treatment Combining Calcium Hydroxide Dissolution and Dehydration With Ambient Air
Water Research X
journal homepage: www.sciencedirect.com/journal/water-research-x
A R T I C L E I N F O A B S T R A C T
Keywords: We present the results of three field tests and three laboratory tests of a new physical-chemical urine treatment
Field testing system, which can recover all nutrients, while pathogens are inactivated. The system consists of two steps. In the
Resource recovery first reactor, biological processes including urea hydrolysis are prevented by mixing fresh urine with calcium
Urine stabilization
hydroxide (Ca(OH)2). Due to the high pH value and the high availability of calcium, phosphate can be recovered
Calcium hydroxide
by precipitation. The high pH value also fosters the inactivation of microorganisms, including pathogens. In the
Source separation
Blue Diversion Autarky second reactor, water is evaporated at low energy consumption by blowing unheated ambient air over the
stabilized urine. Stabilization in the first reactor was successful in all field and laboratory tests. The pH value
remained between 12 and 13, except for short dips due to shortages of Ca(OH)2. Nearly all phosphorus (92-96%)
precipitated and could be recovered as calcium phosphate in the first reactor, while nitrogen and potassium
overflowed with the urine into the evaporation reactor. The efficiency of the second treatment step was very
different for field and laboratory experiments and depended on the duration of the experiment. During a four-day
laboratory test, nitrogen recovery was 98%. In contrast, nitrogen recovery was only around 20% in the long-term
field experiments. The high nitrogen losses occurred, because biological urea hydrolysis was not inhibited
anymore, when the pH value in the second reactor decreased due to the dissolution of high amounts of carbon
dioxide from the ambient air. Potassium was not subject to any significant loss, and the measured recovery in the
solid evaporation product was 98%. Evaporation rates ranged between 50 g m− 2 h− 1 (RH = 82±13%, T =
12±6◦ C) and 130 g m− 2 h− 1 (RH = 60±19%, T = 24±5◦ C) in the three field tests. Apart from some disturbances
due to low supply of Ca(OH)2, the urine module functioned without any substantial failures and was simple to
maintain. The minimum consumption of Ca(OH)2 at full capacity was 6 g⋅L− 1 urine and the electricity demand
was 150 Wh kg− 1 water evaporated from urine, resulting in operational costs of 0.05 EUR pers− 1 d− 1.
* Corresponding author: Kai M. Udert, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland
E-mail address: Kai.Udert@eawag.ch (K.M. Udert).
https://doi.org/10.1016/j.wroa.2021.100124
Received 17 July 2021; Received in revised form 22 September 2021; Accepted 4 October 2021
Available online 8 October 2021
2589-9147/© 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
M.E. Riechmann et al. Water Research X 13 (2021) 100124
the pH increases to values of 12.5 at which the solubility equilibrium for evaporation reactor should allow for a high evaporation rate without the
Ca(OH)2 is reached. At this high pH, biological urea hydrolysis is need for air heating, and separating stabilization and evaporation should
inhibited, phosphate precipitates as amorphous calcium phosphate minimize Ca(OH)2 consumption. Simha et al. (2018) discussed that CO2
(Randall et al. 2016) and most pathogens are quickly inactivated (Sen absorption during evaporation could induce CaCO3 precipitation, which
ecal et al. 2018). However, urine stabilized with base is sensitive to high would lead to an increased Ca(OH)2 consumption. Finally, alkaline
temperatures and pH changes. Randall et al. (2016) recommend to keep stabilization and subsequent water removal was barely tested at field
the temperature below 40◦ C and to maintain pH values below 13 to scale, which is why we included extensive field testing in different lo
prevent chemical urea hydrolysis. Furthermore, they recommend a cations and setups. We expected two major challenges for our setup.
minimum pH value of 11 to suppress biological urea hydrolysis. Around First, limited predictability of evaporation as it strongly depends on
5% of the initial nitrogen leave the body as ammonia and can thus not be climatic conditions. Second, CO2 absorption in the evaporation reactor
stabilized with this method (Udert et al. 2006). could decrease the pH, so that biological urea hydrolysis would become
The first study of a urine treatment system combing alkaline urine possible. However, it was shown that urease, the enzyme responsible for
stabilization and water evaporation with forced convection was re biological urea hydrolysis, is increasingly inhibited at a rising ionic
ported by Dutta and Vinnerås (2016). The two processes, stabilization strength (Hotta and Funamizu 2008, Kumar and Kayastha 2010). We
and water removal were combined in one reactor and a 1:1 mixture of therefore presumed that the increase of salt concentrations during
wood ash and Ca(OH)2 was used as alkaline medium. Since water evaporation would prevent biological urea hydrolysis, even if the pH
removal was very low at ambient air temperatures, the air was heated up decreases below 11.
to 60◦ C, which led to a diminished nitrogen retention of only 46%. The The overall aim of this study was to test whether the urine module of
process was optimized by the same research group using various alkaline the BDAT was suited to concentrate all urine nutrients of a 10 people
calcium- and magnesium-containing materials, such as wood ash (Sen household. We conducted basic laboratory tests for a proof of concept,
ecal and Vinnerås 2017), Ca(OH)2 (Senecal and Vinnerås 2017, Simha controlled short-term tests with the urine treatment module and three
et al. 2020b) and magnesium hydroxide (Vasiljev et al. 2022). Simha field tests, two in Switzerland and one in South Africa. The overall study
et al. (2020b) could show that independently of the alkaline medium, was guided by three basic research questions:
more than 90% of the nitrogen could be recovered, if the pH value in
urine was kept above 12. They could also show that the recovery was i Can urea degradation and subsequent nitrogen loss be prevented?
higher with air at 60◦ C than at 50◦ C, because the faster evaporation rate ii Is the evaporation rate sufficiently high to remove the water from the
at 60◦ C reduced the duration of the experiment. However, in a field collected urine?
experiment with a similar reactor setup (Simha et al. 2020a) only 30% of iii Are the operational costs reasonably low?
the nitrogen was recovered and the electricity demand was high (24.5
kWh kgurine− 1). The high specific electricity demand was caused by the Additionally we determined the mass flows of nitrogen, phosphorus
use of heating fans and an unexpected low urine load. and potassium.
In this study, we tested the urine treatment module of the Blue
Diversion Autarky Toilet (BDAT, www.autarky.ch). The goal of the 2. Materials and Methods
BDAT project was to develop a non-sewered sanitation system for 10
users, which can provide safe sanitation, comfort and resource recovery 2.1. The urine module in the Blue Diversion Autarky Toilet
while operating completely independently of the grid. The urine treat
ment module of the BDAT is also based on alkaline stabilization and The urine module of the BDAT is designed for a 10 people household
evaporation by forced convection. However, there are some substantial or an equivalent of 10 L urine per day and 3 L flushing water per day
differences to the previously reported systems: urine stabilization and (Table 1). The reactor setup consists of two treatment units: urine sta
water evaporation are separated in two sequential reactors, the air is not bilization and water evaporation (Fig. 1). Urine stabilization is achieved
heated and the driving force is instead enhanced through a high air flow. by increasing the pH value through the dissolution of Ca(OH)2. Due to its
This combines with a larger specific surface area in the evaporation limited solubility, just enough Ca(OH)2 dissolves to maintain a pH value
reactor, i.e. 125 m2 m− 3 surface area to volume ratio of the urine of about 12.5. The remaining Ca(OH)2 is available for future urine
compared to 3 m2 m− 3 as in the field tests of Simha et al. (2020a). charges. A Ca(OH)2 amount sufficient to operate the urine module for
Furthermore, we only used pure Ca(OH)2 as base without blending with several weeks is added during each service. The stabilization reactor has
other alkaline substances. We chose this setup with the following in a volume of 25 L and is divided into a mixing and a settling chamber. The
tentions: phosphate should be recovered as a separate high value fer urine is mixed every hour for three minutes at 340 rpm. In the settling
tilizer product in the stabilization reactor, the larger specific area in the chamber, unused Ca(OH)2 and precipitates, mainly calcium and
Fig. 1. Schematic diagram of the BDAT urine module. Fresh urine enters the stabilization reactor (1). The stabilized urine overflows to the evaporation reactor (6).
Ambient air (4) flows through the stacked tray system to force evaporation of water and produce a concentrated product.
2
M.E. Riechmann et al. Water Research X 13 (2021) 100124
Table 1
Testing conditions and reactor specifications of the long-term field tests FTEawag, FTAu and FTDurban.
FTEawag FTAu FTDurban
Location Eawag campus, Dübendorf, Switzerland Peri-urban zone of Au (Zürich), Switzerland Unsewered peri-urban zone of Durban, South
Africa
Setup Urine module test Urine module test Blue Diversion Autarky Toilet
Research focus Mass balances of water and nutrients pH-indicated Ca(OH)2 dosing Operation in a challenging environment
Urine collection External urine collection at Eawag office building, storage at Direct connection to urine diverting dry toilet Direct connection to waterless urinal2 and
4◦ C until use (UDDT)1 water-flushed urine diverting toilet3
Input feed 4.3 L⋅d− 1 fresh urine (pH <7, weekdays) and pre-stabilized Undiluted fresh urine of 2 people Fresh urine of 14 people diluted with toilet
urine (6 gCa(OH)2⋅L− 1, weekends), both diluted with tap flush water
water4 (75:25)
Ca(OH)2 dosing5 800 g through weekly dosing of irregular amounts 180 g dosed if pH <11.5 1800 g at start of each harvest cycle
Duration 28 days (one harvest cycle); August 2019 (late summer) 112 days (one harvest cycle); August to 93 days (two harvest cycles of 43 and 50 days);
November 2019 (late summer to early winter) April to June 2019 (autumn)
Climatic conditions6 Relative humidity (RH): 28-94% (60%) 41-98% (82%) 0-31◦ C (12◦ C) 21-96% (77%) 7-34◦ C (21◦ C)
Temperature: 15-36◦ C (24◦ C)
Sampling Regularly (2-3 times/week): fresh and stabilized urine Stabilized urine Stabilized urine
End: (Triplicate grab samples): Stabilization reactor Stabilization reactor effluent and mixed urine Stabilization reactor effluent, Excess urine and
effluent, excess urine and urine concentrate from trays concentrate from trays urine concentrate from trays
Measurements Constantly: pH in mixer, settler and evaporator (manually), pH in mixer, settler and evaporator (manually), pH in mixer, settler and evaporator (manually),
inflow temperature and RH, inflow masses inflow temperature and RH inflow temperature and RH, air flow, Ammonia
in off-gas
End: Product masses Product masses Product masses
1
Villa 9000, Separett, Värnamo, Sweden.
2
Lema, Laufen Bathrooms, Laufen, Switzerland.
3
Save! prototype 4, EOOS, Vienna, Austria.
4
Simulating toilet flush water entering the system.
5
Ca(OH)2, technical grade (≥95%), VWR Chemicals, Darmstadt, Germany.
6
Ranges with average values in parenthesis during reactor operation hours. For detailed climate conditions see Table S.1 and Figure S.5.
magnesium phosphate minerals, are separated from the liquid and slide Table 2
back into the mixing chamber. To avoid CaCO3 precipitation following Characterization of incoming urine in the field tests given as mean ± standard
CO2 absorption from the air, the stabilization reactor is sealed against deviation. In FTEawag it was possible to test the fresh urine inflow batches, while
incoming air with a membrane valve. in the case of FTAu and FTDurban the sampling took place in the stabilization
In the second treatment unit, water is evaporated from the overflown reactor. For FTDurban, fresh urine was also collected directly from the users
liquid by forced air convection. 12 stainless steel trays (LxW: 930 × 560 during one day to determine the concentrations in the input urine. Parameters
not determined are left blank.
mm2) provide a total evaporation area of 6.2 m2. Urine is distributed to a
lower tray when the water level exceeds a natural overflow of 8 mm, Parameter FTEawag FTAu FTDurban FTDurban
which is integrated at the front of the trays. Nine axial fans (G1238 E 24 Type Diluted fresh Undiluted Undiluted Diluted
B1+6, Mechatronics Inc., Preston, USA) continuously blow ambient air urine stabilized fresh urine stabilized
over the trays during operation of the evaporation reactor. In all long- (75:25)2 urine3 urine
(30:70)2,3
term field tests, the evaporation reactor was operated for 10 h during
Number samples 12 4 Triplicates 11
daytime, in order to take advantage of the lower RH during daytime. To n1
remove malodorous volatile organic gases, a 1 cm thick activated carbon Total organic 4270±870 5860±400 7710±130
filter mat can be inserted at the outflow of the tray stack. When inte carbon (n=11)
grated in the BDAT, the outgoing air is discharged over the roof of the [mg⋅L− 1]
Chemical oxygen 2690±270
toilet cabin. During service, excess urine can be drained by inclining the demand (n=3)
trays and the remaining solid fraction can be harvested with a scraper. [mg⋅L− 1]
To test different influences (e.g. on the energy demand) the air filter at Total nitrogen 4600±1280 6000±470 9250±80 2080±180
the back of the evaporation reactor was removed during selected [mgN⋅L− 1]
Urea [mgN⋅L− 1] 3090±820 4320±410 7640±110
periods.
Total ammonia 143±31 761±146 501±6 160±50
[mgN⋅L− 1]
Nitrate [mgN⋅L− 1] 23±4 (n=2)
2.2. Long-term field tests Nitrite [mgN⋅L− 1] 6±2 (n=6)
Total phosphorus 700±14 8±11 (n=8)
The urine module was tested in three different field settings. One [mgP⋅L− 1]
Orthophosphate 195±65 15±11 660±9
field test was conducted on Eawag campus (FTEawag) with an automatic
[mgP⋅L− 1]
feed of fresh urine in order to control and measure the input of the urine Potassium 1320±390 1560±150 1360±20 510±70
module. The two field tests in Au (FTAu: Zurich/Switzerland) and Dur [mg⋅L− 1]
ban (FTDurban: South Africa), were carried out to evaluate the perfor Sulphate [mg⋅L− 1] 508±114 1330±170 1740±50
mance and adaptability of the module in different real-world contexts. A Chloride [mg⋅L− 1] 2330±280 3570±220 4690±70 1360±120
Sodium [mg⋅L− 1] 1140±180 1990±110 3360±30 940±80
socio-technical evaluation of the field test of the BDAT (including the
Calcium [mg⋅L− 1] 90±55 71±9 640±480
urine module) in Durban is described in Sutherland et al. (2020). Table 1 (n=10) (n=4)
provides further background information about the field experiments Magnesium 73±25 90±4 58±36 (n=6)
(photographs and detailed setup in Figure S.1 to S.4 of the Supple [mg⋅L− 1]
pH 6.5±0.4 12.2±0.4 5.9 12.4±0.2
mentary Information).
To reduce the amount of urine needed for FTEawag, the input volume 1
Deviating sample numbers in brackets due to test kit shortages or invalid
was reduced. The daily inflow of 4.3 L diluted urine corresponded to one measurements.
2
third of the design load. Table 2 gives an overview of the composition of Dilution ratio urine to tap water.
3
Stabilized with Ca(OH)2.
3
M.E. Riechmann et al. Water Research X 13 (2021) 100124
the urine used in the different field tests. minute intervals. In FTDurban, the air flow (monitoring station MKA-R-
DD-160-VRP-M-VFP300, Schako, Givisiez, Switzerland) and ammonia
in the off-gas stream (MONOline 514, Kimessa, Zurich, Switzerland)
2.3. Short-term experiments
were measured online.
In the experiment EvapField, the weight of the evaporation reactor
Three short-term batch experiments were conducted for in-depth
was continuously determined with a scale (IKT 150K2XL, Kern,
studies of the reactor performance. In experiments StabLab (simplified
Balingen-Frommern, Germany) and the measurement results were log
laboratory setup) and StabField (urine module), we focused on the ni
ged in one-minute intervals.
trogen loss due to urea hydrolysis during evaporation. In experiment
EvapField (urine module), we examined the effects of the relative hu
2.5. Sampling
midity (RH) and temperature of the incoming air on the evaporation
rate. Details of all three experiments are summarized in Table 3.
Regular samples were taken from the stabilization reactors during all
field tests. For FTEawag, incoming fresh urine was also sampled and
2.4. Online monitoring weighed. At the end of a harvest cycle, the systems were emptied to
perform mass balances. The excess urine was drained from the trays of
Process stability was measured with pH as a proxy in all field tests. the evaporation reactor, then the remaining solid/liquid mixture, i.e. the
Two pH sensors for pH measurement (Orbisint CPS11D with multi evaporation product, was scraped of the trays. In addition, the urine and
channel transmitter Liquiline CM444, Endress+Hauser, Reinach, the precipitation sediments from the stabilization reactor were drained.
Switzerland) were placed in the mixing and in the settling chamber of The masses of the three fractions excess urine, solid evaporation product
the stabilization reactor, respectively. The pH on the trays of the evap and stabilization drainage effluent were weighted on site (FTEawag:
oration reactor was measured with a portable pH-meter (Multi-340i Champ II, OHaus Europe, Nänikon, Switzerland; FTAu: IKT 150K2XL,
multimeter with SenTix 41-3 pH sensor, WTW, Weilheim, Germany) or Kern, Balingen-Frommern, Germany; FTDurban: CPWplus-35, Adam
with color-fixed indicator stripes (pH-Fix 0-14, Machery-Nagel, Düren, Equipment, Oxford, USA). Triplicate grab samples were taken from each
Germany). mixed and homogenized fraction.
Ambient RH and temperature of the air inflow were measured and
logged with an MSR-145 (MSR Electronics, Seuzach, Switzerland) in ten- 2.6. Analytical Methods
4
M.E. Riechmann et al. Water Research X 13 (2021) 100124
experiments StabField and FTEawag using Equation 4 to Equation 10. The nitrogen volatilization, since the conservative tracer chloride (no
system boundaries were drawn around the stabilization and evaporation degradation and no volatilization during urine stabilization) exhibited a
reactors separately. very similar behaviour as Ntot.
Long-term measurements during the field tests FTAu and FTEawag
mi = Ci ⋅Vi (4)
revealed that the pH continuously ranged between 12 and 13 with the
exception of very few events with lower pH values (Fig. 3). According to
mi,in,stab = mi,vola,stab + mi,outC, + mi,outE, (5)
a previous study by Randall et al. (2016), pH values between 11 and 13
stab stab
mi,outC, = mi,in,evap (6) and temperatures below 40◦ C are optimal for urea stabilization. At pH
stab
values above 11, enzymatic urea hydrolysis is suppressed and significant
mi,in,evap = mi,vola,evap + mi,outE,evap (7) chemical urea hydrolysis requires pH values above 13 or temperatures
above 40◦ C. High temperatures have been the reason for urea hydrolysis
mi,outC,stab + mi,outE,stab and nitrogen loss in previous studies. In field tests with urine stabilized
Ri,stab = (8)
mi,in, stab with base, Simha et al. (2020a) reported nitrogen recoveries of only
30±6% when using 60◦ C hot air for evaporation. Dutta and Vinnerås
Ri,evap =
mi,outE,evap
(9) (2016) reported nitrogen recoveries of 74% and 54% using temperatures
mi,in, evap of 35◦ C and 60◦ C. However, it seems to be possible to also limit the
chemical urea hydrolysis and Ntot loss below 10% at temperatures up to
mi,outE,stab + mi,outE,evap
Ri,tot = (10) 60◦ C when using high air flow rates and thereby reducing the time while
mi,in, stab
urine is exposed to high temperatures (Simha et al. 2020b).
We tested two different strategies to ensure the required high pH
With parameters
values. The most reliable pH control was achieved in FTDurban, when all
m: total mass of one experimental cycle
Ca(OH)2 (1.8 kg) predicted to be required for the whole field test was
C: concentration
added in the beginning. Due to the high dosage, there was no problem
V: total volume of one experimental cycle
with low pH values during the whole experiment. To increase the dosing
R: recovery efficiency and indices
efficiency and to reduce the amount of residual Ca(OH)2 in the end
i: Ntot, total phosphorus, potassium or water
product, a different dosing regime was tested in FTAu, where Ca(OH)2
Ntot: total nitrogen
was refilled when the pH value dropped below 11.5. Our experiments
Cl: chloride
showed that short periods with pH values below 11 can occur with the
in: total input to one reactor during one experimental cycle
intermittent dosage regime, but they did not cause substantial urea
outC: total output that leaves the stabilization reactor continuously
degradation. Between days 10 and 20, the pH values in FTAu showed
during one experimental cycle
strong fluctuations. However, the frequent pH drops were most probably
outE: total accumulated reactor content at the end of each experi
due to enzymatic urea hydrolysis in a siphon, installed in front of the
mental cycle
stabilization reactor. Longer urine retention times in the siphon, e.g. at
vola: volatilized
night, might have favored enzymatic urea hydrolysis and thereby the
stab: stabilization reactor
release of ammonium and bicarbonate, which act as acids in solutions
evap: evaporation reactor
with high pH values. The strong fluctuation and low pH values did not
occur anymore after the siphon was removed on day 23. This incident
3. Results and discussion
highlights that the fresh urine must be transported quickly into the
stabilization reactor to prevent urea hydrolysis.
3.1. Nutrient stabilization and recovery
Fig. 2. Total nitrogen (Ntot) and ammonia (NHtot) concentrations in the stabilization reactor during the field tests FTAu and FTDurban. The tracer chloride (Cl) is given
as a reference for inflow concentration variations.
5
M.E. Riechmann et al. Water Research X 13 (2021) 100124
Fig. 3. Online monitoring of the stabilization reactor during the field tests FTAu and FTDurban. The reactors’ mixing compartment is represented by pHmixer the settling
compartment by pHsettler. The critical limit for enzymatic urea hydrolysis is pH 11. Arrows indicate refilling of Ca(OH)2 and urine.
recovery (Figure S.7). Only about the amount of NHtot present before all three field tests with values decreasing from initial pH values around
evaporation (130 mgN⋅L− 1) volatilized during the evaporation of stabi 12.5 to values between 8 and 10 on all trays. Concomitant with the pH
lized urine, resulting in a total N loss of 3%. A second batch run of the value, the nitrogen concentration given in relation to the tracer chloride
urine module fed with stored urine (NHtot,in=4600 mgN⋅L− 1) showed decreased. In all experiments, the ratio of NHtot:Cl was much lower than
that without stabilization, nearly all nitrogen (97%) will be lost: in this the Ntot:Cl ratio as long as the pH was above the critical value of 11 but
experiment, the Ntot concentration decreased from an initial concen close to the ratio of Ntot:Cl for lower pH values. The strong increase of
tration of 6300 mgN⋅L− 1 to 200 mgN⋅L− 1 within four days (Table S.3). the NHtot concentration can be explained by the biological urea hydro
This clearly shows the importance of stabilization for nitrogen recovery. lysis, which can occur at pH values below 11 (Randall et al., 2016). In all
In contrast to the results of the short-term evaporation tests, a sub field tests, the pH values were lower in the lower trays (Fig. 6), which
stantial amount of nitrogen was lost during the long-term field tests means that the pH values decreased with the hydraulic retention time:
(Fig. 4, see Table S.4 for data). After around 10 to 20 days, depending on the urine enters the reactor on the top and has to flow over all trays.
the sampled tray, the pH dropped and urea hydrolysis started (Fig. 5). The lower pH values in the long-term experiments can be explained
The recovery of nitrogen decreased to around 20% after one month with the increased amounts of CO2 from the ambient air, which dis
(FTEawag and FTDurban) and even to 10% after three months (FTAu). The solved in urine and lead to a pH decrease. As a comparison, in the
recovery efficiencies for the stabilization reactor, however, were close to experiment StabField 22 000 m3 of air was blown over the trays during
100% for FTEawag (92%) and FTAu (94%) and 78% for FTDurban. The the operation time, while in the field experiments, the air volumes
reason for the lower nitrogen recovery efficiency during stabilization in ranged between 144 000 and 183 000 m3, being 6.5 to 8.3 times as high.
FTDurban is unclear, since no substantial urea hydrolysis had occurred A more detailed calculation of the CO2 absorption and the concomitant
during stabilization (see Fig. 2). One reason could be that the values pH decrease would be beneficial, however, to do that it would be
used for fresh urine in the calculation of FTDurban, were not represen necessary to determine the exact kinetics of CO2 absorption in the urine
tative: as it was not possible to measure the fresh urine during the field module first.
test, a once off collection campaign in the same household served as a According to Brison (2016), the strong pH decrease caused by CO2
basis. dissolution is a combination of proton release from dissolved CO2,
The pH measurements on the trays (Fig. 5) showed a similar trend in alkalinity consumption by CaCO3 precipitation, biological urea
6
M.E. Riechmann et al. Water Research X 13 (2021) 100124
Fig. 5. Development of pH (first row) and total nitrogen (Ntot), respectively total ammonia (NHtot) to chloride (Cl) ratios (second row) inside the evaporation reactor
during field tests FTEawag, FTAu and FTDurban. ● and + indicate measurements on the 1st, respectively the 6th tray. The values at day 1 were measured in the overflow
into the evaporation reactor (reference point).
hydrolysis when the pH decreases and, finally, ammonia volatilization. discussed in section 3.1.1. While FTEawag and FTDurban also had signifi
Since urease and urease-producing bacteria were most probably deac cant nitrogen loss, no malodor was reported. Online ammonia off-gas
tivated during the stabilization phase (Geinzer 2017), it is likely that measurements in FTDurban constantly showed values below the detec
new urease was reintroduced to the evaporation system with ambient air tion limit of 8 mg m-3 (see Figure S.8), which is below the Swiss
during aeration. maximum concentration at the workplace (long-term) of 14 mg m-3
The pH decrease due to CO2 dissolution can be delayed if residual (SUVA 2016). Following power cuts of more than 12 h, there were only
Ca(OH)2 is available. However, this also means that CO2 dissolution two exceptions where ammonia concentrations at night time shortly
consumes Ca(OH)2. Saving Ca(OH)2 was actually one of the reasons, reached 670 and 200 mg m-3 (10 min interval) inside the reactor. The
why stabilization with Ca(OH)2 was separated from evaporation in this low average ammonia concentration in the air can be validated based on
study. A single reactor system as operated by Dutta and Vinnerås the nitrogen mass balance. Dividing the nitrogen loss (250 g) by the total
(2016) and Senecal and Vinnerås (2017) has the advantage of a direct air throughput (183 000 m3) results in an average ammonia concen
compensation of the introduced CO2 by Ca(OH)2 dissolution. However tration in the air of 0.6 mg m− 3. This calculation shows that there was a
this comes at the cost of high consumption of Ca(OH)2 (see section high dilution of the contaminant in the air and no risk for the users, even
3.3.2). in case of a decreased stabilization rate. To reduce the emissions to the
Occasional malodor was reported for the field test FTAu. The cause air as well as the nutrient loss, two measures can be taken: (i) increase
were most likely volatile nitrogen or organic carbon compounds the amount of residual Ca(OH)2 in the evaporation reactor, or (ii) reduce
(Troccaz et al. 2013) and could be attributed to the hidden siphon the CO2 introduced to the system. These measures are being considered
in the further development of the technology.
7
M.E. Riechmann et al. Water Research X 13 (2021) 100124
waiting time until the urine is completely dried out and the urine water was removed (Fig. 7). Overall, water removal was similar for the
module produces zero effluent for discharge, which could pollute the other long-term field experiments that is 92% for FTAu and 93% for
environment. FTDurban. The removal rates, however, varied strongly. Between 0.54 and
1.28 kgwater m− 2wetted surface were removed in one day, when the fans
were operated for 10 hours per day. The variations can be explained
3.2. Water removal
with differences in the climatic conditions (Table 4). Throughout all
field tests the urine module never reached full capacity with the urine
During the long-term field test FTEawag about 96% of the incoming
Fig. 7. Overall mass flows of water and major nutrients being recovered in experiment EvapField and field test FTEawag. FTEawag was run at 50% of its capacity. The
part of the flow scheme not leaving the first system boundary is the fraction that is intermediately stored in the stabilization reactor.
8
M.E. Riechmann et al. Water Research X 13 (2021) 100124
inflow being the limiting factor, which explains the low daily water experiments.
removal. Maximum efficiency was thus just reached in the short term The average evaporation rates for the urine module (Table 4)
experiment EvapField. Taken the evaporation rate determined in Evap compare favorably with results from previous studies: Based on the
Field and extrapolating it to 12 trays and 24 h operation, the daily water linear fit of the experimental data (EvapField) at 70% RH and 20◦ C, the
removal would have been 19.8 kg d− 1. This value is well above the urine module would evaporate around 94 g m− 2 h− 1. It shows that a
design value of 13 kg d− 1 for the Blue Diversion Autarky Toilet. How system operated at a high airflow can compensate for the high RH. For
ever, it should be noted that at night, the RH is usually higher and the comparison, Bethune et al. (2016) reported 110 g m− 2 h− 1 at 22% RH
actual evaporation rate for 24 h operation would have been lower. and 20◦ C. At the same conditions of 70% RH and 20◦ C, Dutta and
Nevertheless, the capacity of the urine module is close if not above the Vinnerås (2016) evaporated 8 g m− 2 h− 1. Both research groups operated
design value. In areas prone to droughts it might be useful to include at low airflows per evaporation surface, 5.5 m3 m− 2 h− 1 and 1.7 m3 m− 2
another step to recover the evaporated water. Water recovery, however, h− 1, respectively, which were substantially lower than the 130 m3 m− 2
comes at the price of higher system complexity and higher energy h− 1 in our experiments. Another possibility to increase evaporation is
demand. heating the incoming air. By using simple solar dehydration, Antonini
The experiment EvapField showed that the evaporation rate of water et al. (2012) achieved 42 g m− 2 h− 1 (RH not provided, 50-60◦ C). During
from stabilized urine correlated with the ambient RH (Fig. 8). Further short-term tests with pre-heating the air to 50◦ C and with 20% RH
more, the evaporation rate depended on the ambient temperature. At Simha et al. (2018) achieved a very high evaporation rate of 500 g m− 2
higher temperatures, the water holding capacity of the air is increased. h− 1. However, heating results in substantially higher energy demands of
Consequently, higher temperatures at the same RH lead to an increased 5 to 30 times compared to the use of high airflow at ambient tempera
evaporation rate. Also the mass transfer from the water to the gas phase ture (see discussion in section 3.3.1).
increases with the temperature, in accordance with the increase of the
diffusion coefficient of water vapor in air. The measured data could be
described sufficiently well with a model according to Equation 11, 3.3. Resource requirements
which considers the difference between 100% and the measured RH in
the ambient as driving force and a mass transfer coefficient which de 3.3.1. Energy
pends on the temperature in the incoming air (Fig. 8) The average daily electrical energy demand for the urine module
[ ] tested in the field (FTDurban) was 1260±270 Wh d− 1 and corresponded
E = km,T0 ⋅e0.0063⋅(T− T0 ) ⋅(1 − RH) g⋅m− 2 ⋅h− 1 (11) well with the expected demand of 1354 Wh d− 1 (stirrer in the stabili
zation reactor: 58 Wh d− 1 and fans in the evaporation reactor: 1296
With
kWh d− 1). As the two energy consuming appliances are operated inde
E: evaporation rate
pendently of the urine inflow, the relative energy demand per kg water
km,T = km,T0 ⋅eb⋅(T− T0 ) : mass transfer coefficient [g m− 2 h− 1] evaporated was determined by the urine inflow, i.e. the used reactor
km,T0 =3.29 g m− 2 h− 1: mass transfer coefficient at T0 capacity, and on the evaporation rates, which in turn depended on the
eb⋅(T− T0 ) : temperature correction assuming diffusion depends expo RH of the incoming air (see Fig. 8). A comparison of different reactor
nentially on temperature [-] settings and their specific energy demands is given in Table 5.
b=0.0063: temperature correction exponent [◦ C− 1] interpolated The specific energy demand of our system is much lower than the
from values for water vapor diffusion in air (Lide 2009) theoretical value of 710 Wh kgH2Oevap− 1 required for water evaporation
T: temperature [◦ C] by heating (Udert and Wächter 2012). In the experiment EvapField it was
T0=20◦ C: reference temperature actually close to 110±30 Wh kgH2Oevap− 1, which was reported for water
1 − RH: driving force for evaporation [-] removal from nitrified urine in a distiller with vapour compression and
RH : relative humidity [-] heat recovery (Fumasoli et al. 2016). While in the system used by
Fumasoli et al. (2016), the low energy demand was achieved by
The basic assumptions of Equation 11, however, are not correct ac recovering heat during vapour condensation, the lower energy demand
cording to the theory of evaporation. The driving force is actually the of our system can be explained by capturing heat from the ambient air,
difference between the actual and the maximum achievable absolute which is cooled during the evaporation process. This is supported by
humidity of the air in the reactor. Furthermore, the relevant temperature measurements during FTAu, showing an average temperature difference
is the temperature at the water/air interface (Bergman et al. 2011). of 0.7±1.1◦ C between air in- and outflow (at an average Tin = 12±6◦ C).
Since these data were not available to us, we used relative humidity and A comparison in Table 5 suggests that air pre-heating (65◦ C, Senecal
temperature measurements of the incoming air. Apparently, using the 2020) without heat recovery at a low air flow (~30 m3 h-1 m-2, Simha
temperature and RH of the incoming air compensate for the deviations et al. 2018) may be energetically less favourable, by at least a factor of
from theory. A fit with the absolute humidity of the incoming air did 10, than increasing the air flow (130 m3 h− 1 m− 2, this study) using
result in worse correlations (see residuals in Figure S.9 and Figure S.10). ambient air. A disadvantage of a high air flow is the faster dissolution of
The approach used in Equation 11 might therefore be useful in practice, CO2 from the air, leading to a pH decrease and subsequently to enzy
when absolute humidity in the reactor and the temperature at the matic urea hydrolysis as discussed in section 3.1.2. The use of heat,
water/air interface cannot be easily determined as it was the case in our however, favours chemical urea hydrolysis.
Table 4
Mean evaporation rates and water removal at 10 h operation per day obtained in the long-term field tests FTEawag, FTAu and FTDurban and in the short-term experiment
EvapField. Relative humidity (RH) and temperature are represented as the average values during the active operation hours (9:00-19:00) during the field tests.
RH Temperature Evaporation rate Available trays Reactor capacity used Daily water removal (10h operation)
[%] [◦ C] [g m− 2 h− 1] [# trays] [kg d− 1]
9
M.E. Riechmann et al. Water Research X 13 (2021) 100124
Fig. 8. Evaporation rate as a function of relative humidity (RH) and temperature in the incoming air measured in experiment EvapField. Higher temperature and
lower RH result in a higher evaporation rate. The regression lines were fitted with the evaporation and RH data at the temperatures 10◦ C (dotted), 20◦ C (line) and
30◦ C (dashed).
Table 5
Specific energy demand for different urine dehydration systems
BDAT urine module1 Alkaline dehydration in ash Distillation
For an efficient energy use, it is important to know the amount of urine stabilization. This is lower than the 10 g⋅L− 1 suggested by Randall
urine inflow and thus the used reactor capacity. Due to its modular et al. (2016), who, however, considered a safety factor of 2. When using
structure the urine module can be adjusted to three different programs, 6 g⋅L− 1 around 22 kg of Ca(OH)2 per year would be necessary for a
using 3 fans with 4 trays, 6 fans with 8 trays or 9 fans with 12 trays. A treatment rate of 10 Lurine d− 1. Compared to other reported on-site urine
sophisticated process control, taking into account the urine inflow, treatment systems, for which acid or base was used for urine stabiliza
could also strongly reduce the energy demand. However, such a process tion, this is a rather small amount of additive and the costs are low
control needs to be sufficiently robust for the challenges of on-site ap (Table 6).
plications in locations with low technical service. Further energy Adding up the operational costs of 18 EUR pers− 1 yr− 1 for electricity
reduction potential lies in the reduction of friction for the fans. The and 0.70 EUR pers− 1 yr− 1 for Ca(OH)2 we end up at daily costs of 0.05
activated carbon air filter mat did not show a high benefit during the EUR pers− 1 d− 1. This value is in the same range as the 0.05 USD pers− 1
field tests, as it could not prevent a malodour event in FTAu. By removing d− 1, which was set as challenging task by the Bill and Melinda Gates
it, the consumed energy could be reduced by 17% (from 420 to 350 Wh Foundation for the overall costs of non-sewered sanitation systems. The
kgH2Oevap− 1) during FTDurban. Removing the filter also increased the overall costs, however, would be higher for the urine module and even
airflow from 800 to 1050 m3 h− 1 (Figure S.11), which is close to the more for the whole BDAT. A major cost factor not considered for the
originally aimed design value (1100 m3 h− 1) and increased the evapo urine module could be the service by a technician. However, selling
ration efficiency. fertilizer might result in some revenue. This revenue cannot be quanti
fied at the moment, because the costs for a possible post-treatment of the
3.3.2. Additives fertilizer and the market price of the final product are not known yet.
1
Our experiments showed that 6 gCa(OH)2⋅Lurine− are sufficient for
10
M.E. Riechmann et al. Water Research X 13 (2021) 100124
Table 6
Comparison of required additive amounts and costs of different small-scale urine treatment systems
Ca(OH)2 Mixed ash & lime Alkaline ash treatment Magnesium Acid treatment 1 Acid treatment 2
treatment treatment treatment
Additive Ca(OH)2 Wood ash, Ca(OH)2 (1:1) Wood ash Mg(OH)2, MgCl2 Sulphuric acid Phosphoric acid
(3:1) (96%) (89%)
1 1 1
Dosing Passive Passive Passive Passive1 Active2 Active2
Spec. amount 6 100-2903 503 22 27.76 [mL⋅L− 1] 31.46 [mL⋅L− 1]
[g⋅Lurine− 1]
Annual amount4 22 365-1058 183 80 101 [L yr− 1] 115 [L yr− 1]
[kg yr− 1]
Cost5 [EUR pers− 1
0.20-0.70 2-176 06 1-25 61-390 9-108
yr− 1]
Reference This study (Dutta and Vinnerås (Senecal and Vinnerås (Vasiljev et al. 2022) (Antonini et al. (Antonini et al. 2012)
2016) 2017) 2012)
1
Reservoir of solids with low solubility inside reactor.
2
External liquid dosing device.
3
Calculated based on total amounts of additive per volume urine applied.
4
Calculated assuming urine treatment of 10 L d− 1 during 365 d.
5
All costs displayed show an order of magnitude; the price calculation (Table S.5) varies significantly according to origin and purchased entity.
6
Assuming ash is a waste product and thus adds no costs.
3.4. Fertilizer quality CO2 absorption is most probably driving the pH decrease, which in
turn results in biological urea hydrolysis. To tackle this issue, two
3.4.1. Nutrient availability for plants measures can be taken: (a) allow excess Ca(OH)2 to enter the evap
In a previous study by Meyer et al. (2017), the plant availability of oration reactor, or (b) regulate the CO2 input through the air.
phosphorus in alkaline soils was tested for two possible products from • Water could be removed reliably at ambient air temperature without
our process: the precipitation product from the stabilization reactor and additional heating by just using a high airflow as the main driver.
a blend of the two products from the stabilization and the evaporation The water removing capacity is closely linked to the RH of the
reactor. Both potential fertilizers were produced from real urine in the incoming air. At 20◦ C, 55% RH and 24h operation of the fans, the
laboratory. The plant availability of phosphorus was 22 and 22.9 mgP water removing capacity would be 20 kg d− 1, which is substantially
kgSoil− 1, for the stabilization reactor product and the blended product, above the target value of 13 kg d− 1. If operated outside, low tem
respectively. These values were very similar to the values for water peratures and high RH at night can reduce the water removing
soluble phosphorus (23.9 mgP kgSoil− 1) and around 30% higher than for capacity.
sewage sludge ash (15.3 mgP kgSoil− 1). These results were promising, but • The operational costs are expected to be reasonably low. The energy
further studies on the plant availability of nutrients besides phosphorus and the additive demand resulted in estimated costs of 0.05 EUR
and on possible effects of salt and unwanted substances such as phar pers− 1 d− 1. The performance of the system and thereby the opera
maceuticals are needed for an overall assessment of the fertilizer quality tional cost strongly depended on (a) the used reactor capacity and (b)
of the products from the urine module. Depending on the results of this the RH of the incoming air.
assessment, further processing might be necessary. • Phosphate was recovered as a separate product from the stabilization
reactor. Depending on the market demand, this product could be
3.4.2. Pathogen removal used independently or be blended with the second mixed nutrient
Pathogens testing at the end of one harvest cycle in FTDurban suggests product from the evaporation reactor or other fertilizers. Revenues
that there is no contamination with fecal bacteria in the concentrated from fertilizers could offset operational costs.
evaporation product: E. coli and total coliforms were both below 1 MPN
(most probable number)/100 mL. Furthermore, no helminths were CRediT authorship contribution statement
detected in the evaporation product, and only one undeveloped Ascaris
suum egg was determined in a 500 mL sample of the stabilization reactor Michel E. Riechmann: Conceptualization, Methodology, Investi
(see Table S.6). To be certain that helminths are effectively removed, gation, Formal analysis, Writing – original draft, Writing – review &
spiking tests are suggested, as the naturally applied helminth load dur editing, Visualization. Bonginkosi Ndwandwe: Investigation, Writing –
ing the field tests is unknown. While for bacteria and viruses, Senecal review & editing. Esther E. Greenwood: Investigation, Writing – review
et al. (2018) confirmed a complete inactivation after four days in their & editing. Eva Reynaert: Investigation, Writing – review & editing.
study on urine stabilized with base at a pH of above 10.5 (20◦ C), it took Eberhard Morgenroth: Writing – review & editing, Supervision. Kai M.
166 days to reach 3 log10 reductions of helminths (Ascaris suum). At a Udert: Conceptualization, Writing – review & editing, Supervision,
temperature of 42◦ C, the time to reach 3 log10 reductions was reduced to Project administration, Funding acquisition.
nine days. As we run our system at pH 12.5, it is advised to redo spiking
tests for this pH level. However, the majority of helminths are excreted
with feces. To comply with hygiene standards of the WHO (2006) and Declaration of Competing Interest
USEPA (1994) for human waste treatment, source separation alone
reaches a 5.2 log10 reduction of Ascaris suum (Senecal et al. 2018). The authors declare that they have no known competing financial
interests or personal relationships that could have appeared to influence
4. Conclusions the work reported in this paper.
11
M.E. Riechmann et al. Water Research X 13 (2021) 100124
Acknowledgements Lide, D.R. (ed.), 2009. CRC Handbook of Chemistry and Physics, CRC Press / Taylor and
Francis, Boca Raton, FL, USA.
Meyer, G., Frossard, E., Mäder, P., Nanzer, S., Randall, D.G., Udert, K.M., Oberson, A.,
We would like to thank the families that tested the urine treatment 2017. Water Soluble Phosphate Fertilizers for Crops Grown in Calcareous Soils – an
system in Au and Durban, as well as all the Eawag staff providing us with Outdated Paradigm for Recycled Phosphorus Fertilizers? Plant and Soil 424 (1-2),
urine for the testing and with feedback on the system. Richard Fank 367–388.
Brison, A. (2016) Understanding the Processes Involved during the Stabilization of Urine
hauser, Sylvia Richter and Karin Rottermann were a great support with Calcium Hydroxide, Institute of Environmental Engineering, Swiss Federal
during installation of the Blue Diversion Autarky system and for the Institute of Technology Zurich (ETHZ), Zurich, Switzerland.
laboratory analyses at Eawag. Chris Buckley, Susan Mercer, Rebecca Randall, D.G., Krähenbühl, M., Köpping, I., Larsen, T.A., Udert, K.M., 2016. A Novel
Approach for Stabilizing Fresh Urine by Calcium Hydroxide Addition. Water
Sindall, Couben Govender, Merlien Reddy and the whole team of the Research 95, 361–369.
WASH R&D Center kindly hosted us during the field test in Durban. We Senecal, J., Nordin, A., Simha, P., Vinnerås, B., 2018. Hygiene Aspect of Treating Human
are also grateful to Ruth Cottingham and Philisiwe Boyce from Khanyisa Urine by Alkaline Dehydration. Water Research 144, 474–481.
Senecal, J., Vinnerås, B., 2017. Urea Stabilisation and Concentration for Urine-Diverting
Projects, who prepared the location for the field test in Durban. Dry Toilets: Urine Dehydration in Ash. Science of the Total Environment 586,
650–657.
Supplementary materials Senecal, S. 2020. Safe Nutrient Recovery from Human Urine - System and Hygiene
Evaluation of Alkaline Urine Dehydration. Doctoral Thesis. Swedish University of
Agricultural Sciences (SLU), Department of Energy and Technology, Uppsala,
Supplementary material associated with this article can be found, in Sweden.
the online version, at doi:10.1016/j.wroa.2021.100124. Siegrist, H., Laureni, M., Udert, K.M., 2013. Transfer into the gas phase: ammonia
stripping. In: Larsen, T.A., Udert, K.M., Lienert, J. (Eds.), Source Separation and
Decentralization for Wastewater Treatment. IWA Publishing, London, UK, pp.
Literature 337–350.
Simha, P., Karlsson, C., Viskari, E.-L., Malila, R., Vinnerås, B., 2020a. Field Testing a
Antonini, S., Nguyen, P.T., Arnold, U., Eichert, T., Clemens, J., 2012. Solar Thermal Pilot-Scale System for Alkaline Dehydration of Source-Separated Human Urine: A
Evaporation of Human Urine for Nitrogen and Phosphorus Recovery in Vietnam. Case Study in Finland. Frontiers in Environmental Science 8, 619901.
Science of the Total Environment 414, 592–599. Simha, P., Lalander, C., Nordin, A., Vinnerås, B., 2020b. Alkaline Dehydration of Source-
Bergman, T.L., Incropera, F.P., DeWitt, D.P., Lavine, A.S., 2011. Fundamentals of Heat Separated Fresh Human Urine: Preliminary Insights into Using Different
and Mass Transfer. Wiley. Dehydration Temperature and Media. Science of the Total Environment 733,
Bethune, D.N., Chu, A., Ryan, M.C., 2014. Passive Evaporation of Source-Separated Urine 139313.
from Dry Toilets: A Lab Study. Journal of Water, Sanitation and Hygiene for Simha, P., Senecal, J., Nordin, A., Lalander, C., Vinnerås, B., 2018. Alkaline Dehydration
Development 4 (4), 654. of Anion–Exchanged Human Urine: Volume Reduction, Nutrient Recovery and
Bethune, D.N., Chu, A., Ryan, M.C., 2016. Passive Evaporation of Source-Separated Urine Process Optimisation. Water Research 142, 325–336.
from Dry Toilets: Ues Optimization and Dry Product Accumulation over Time. Sutherland, C., Reynaert, E., Dhlamini, S., Magwaza, F., Lienert, J., Riechmann, M.E.,
Journal of Water, Sanitation and Hygiene for Development 6 (1), 96–103. Buthelezi, S., Khumalo, D., Morgenroth, E., Udert, K.M., Sindall, R.C., 2020. Socio-
Dutta, S., Vinnerås, B., 2016. Fertilizer from Dried Human Urine Added to Ash and Lime - Technical Analysis of a Sanitation Innovation in a Peri-Urban Household in Durban,
a Potential Product from Eco-Sanitation System. Water Science and Technology 74 South Africa. Science of the Total Environment 755, 143284.
(6), 1436–1445. SUVA, 2016. Grenzwerte am Arbeitsplatz 2016. Swiss National Accident Insurance Fund
Friedler, E., Butler, D., Alfiya, Y., 2013. Wastewater composition. In: Larsen, T.A., Udert, (SUVA), Lucerne, Switzerland. (In German: Workplace exposure limits 2016).
K.M., Lienert, J. (Eds.), Source Separation and Decentralization for Wastewater Troccaz, M., Niclass, Y., Anziani, P., Starkenmann, C., 2013. The Influence of Thermal
Treatment. IWA Publishing, London, UK, pp. 259–273. Reaction and Microbial Transformation on the Odour of Human Urine. Flavour and
Fumasoli, A., Etter, B., Sterkele, B., Morgenroth, E. and Udert, K. M. (2016). "Operating a Fragrance Journal 28 (4), 200–211.
Pilot-Scale Nitrification/Distillation Plant for Complete Nutrient Recovery from Udert, K.M., Larsen, T.A., Gujer, W., 2006. Fate of Major Compounds in Source-
Urine" Water Science and Technology 73(1): 215-222. Separated Urine. Water Science and Technology 54, 413–420.
Geinzer, M., 2017. Inactivation of the Urease Enzyme by Heat and Alkaline pH Udert, K.M., Wächter, M., 2012. Complete Nutrient Recovery from Source-Separated
Treatment. Masters thesis. Swedish University of Agricultural Sciences (SLU), Urine by Nitrification and Distillation. Water Research 46, 453–464.
Departmnet of Energy and Technology, Uppsala, Sweden. USEPA, 1994. A Plain English Guide to the EPA Part 503 Biosolids Rule. U.S.
Hotta, S., Funamizu, N., 2008. Inhibition Factor of Ammonification in Stored Urine with Environmental Protection Agency, Office of Wastewater Management, Washington,
Fecal Contamination. Water Science Technology 58 (6), 1187–1192. DC, USA.
Kumar, S., Kayastha, A.M., 2010. Inhibition Studies of Soybean (Glycine Max) Urease Vasiljev, A., Simha, P., Demisse, N., Karlsson, C., Randall, D.G., Vinnerås, B., 2022.
with Heavy Metals, Sodium Salts of Mineral Acids, Boric Acid, and Boronic Acids. Drying Fresh Human Urine in Magnesium-Doped Alkaline Substrates: Capture of
Journal of Enzyme Inhibition and Medicinal Chemistry 25 (5), 646–652. Free Ammonia, Inhibition of Enzymatic Urea Hydrolysis & Minimisation of Chemical
Larsen, T.A., Riechmann, M.E. and Udert, K.M., 2021. State of the art of urine treatment Urea Hydrolysis. Chemical Engineering Journal 428, 131026.
technologies: A critical review. Water Research X 13, 100114. WHO, 2006. The Use of Excreta and Greywater in Agriculture, 4. World Health
Larsen, T.A., Udert, K.M., Lienert, J., 2013. Source Separation and Decentralization for Organization, Geneva, Switzerland.
Wastewater Management. IWA Publishing, London, UK.
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