Performance of S.parkle during batch culture (mean of 4 runs) Performance of S.

parkle during batch culture (mean of 4 runs)

Start density: 750 rotifers/ml Start density: 1.000 rotifers/ml

.parkle
2300 35,0 3000 35

2100
30,0 30

selco®
2500
1900
25,0 25
1700
2000
1500 20,0 20
Rots/ml

Rots/ml
% eggs

% eggs
1300 15,0 15
1500
1100
10,0 10
900

700
5,0
1000
5 A NATURAL CULTURE DIET
500
T0 T24 T48 T72
0,0 500
T0 T24 T48 T72
0 FOR A SPARKLING, CONSISTENT AND PERFORMANT ROTIFER CULTURE

Economic
S.parkle is cost-effective. Only 1,1 g of S.parkle is necessary to produce 1 million rotifers against 1,4 g of a standard culture diet.

TYPICAL COMPOSITION

Moisture 5%
Crude protein 39%
Crude ash 5%
Crude lipids 12%
Crude fiber 1%
Clean
Phosphorus 1% S.parkle has an optimal behaviour in the culture tank, the surface is sparkling clean, without foam
Vit.A 500.000 IU/kg or flock formation, for a constant rotifer performance.
Vit.D3 50.000 IU/kg
Vit.E 3.600 mg/kg Natural
Vit.C 4.000 mg/kg
S.parkle is formulated with high quality protected components and purified ingredients following
Antioxidants Ethoxyquin, BHA, propylgallate
a strict quality control protocol, leading to a consistent product aspect.
∑ω3HUFA 17 mg/g dwt
DHA/EPA 3 Consistent & productive
S.parkle is suitable for very short and productive cycles in any condition, allowing rotifer generations
to be re-inoculated up to 50 consecutive runs.
PACKAGING & STORAGE
Economic
Selco® S.parkle is available in 4 x 2,5 kg alufoils.
S.parkle is cost-effective and easy to use.
S.parkle has a shelf life of 12 months from date of manufacture. The product should be stored in a cool, dry place (max. 10°C). Once opened, it should be
used within one month, kept well closed and stored in a refrigerator.
Do not freeze.
TC-EU-S.01-0409

INVE Aquaculture, Hoogveld 93, B-9200 DENDERMONDE - BELGIUM Tel +32 (0) 52.40.95.95 Fax +32 (0) 52.40.95.86 inveaqua_bu@inve.be
 USE

1.1 Preparation and use of the product Low ciliate contamination

Contamination starts +/- 1 day later, ciliates are less in number, which makes filtration easier and faster.
• Blend Selco® S.parkle during three to five minutes in freshwater with an industrial mixer using a maximum of 100 g per litre. The graph represents relative number of ciliates found in a standard culture diets vs S.parkle
• Create a vortex first and then slowly add S.parkle to the water.
• Start with an inoculum of between 250 and 1.000 rotifers per ml. Apply according to culture strategy below.

1.2 Preparation of the tank

• Clean and disinfect the culture tank, airstones, air tubing prior to use (use e.g. 200 g/m³ active chlorine + detergent).
• Disinfect the water of the tank with e.g. 5 g/m³ active chlorine and aerate gently for ± 1 hour.
• Deactivate any remaining chlorine by adding 7 g/m³ sodiumthiosulphate.
• Suspend airstones 15 cm above the tank bottom, along the periphery and also in the centre to allow sedimentation and flushing of waste particles.
• Gentle aeration through the use of airstones; sufficient to keep the rotifers in suspension but at the same time allowing debris to deposit.
• The use of floc traps, e.g. Scotch brite, is advised to ensure a clean culture by the removal of waste floccules and Vorticella. Use a minimum of
2*0,15 m² Scotch brite per m³.

Natural
1.3 Tank set-up and optimal culture conditions
S.parkle is formulated with high quality protected algal components and purified ingredients following a strict quality control protocol, leading to a
consistent product aspect with a nice green algae-like colour.
• Temperature 25-27°C
• Salinity 20-25 ppt
• Dissolved oxygen 5-7 ppm
• NH4+ < 20 ppm; NH3 < 1 ppm
• pH 7 – 8,5
• Aeration reasonably strong using airstones
• The use of additional oxygenation systems is essential to maintain a minimum oxygen level of 4ppm during the entire culture period.

1.4 Culture FEEDING strategy

Rot/ml g S.parkle / million Rot

250 - 500 0,65
Consistent & productive
500 - 750 0,50
S.parkle s suitable for very short and productive cycles in any condition. The product ensures a constant and stable rotifer production, allowing
750 - 1.000 0,45
rotifer generations to be re-inoculated up to 50 consecutive runs.
1.000 - 1.500 0,40
1.500 - 2.000 0,35 Stability of rotifer growth in a three day batch culture (density at start 500 Rot/ml) SGR of rotifers in a 3 days batch culture
2.000 - 3.000 0,30 1500 46

growth (million rotifers reproduced/m3)

44
Feed the daily quantities in 4 to 6 rations or use a continuous feeding device. 1400
42
On the first culture day, start with 0,7 g S.parkle per million rotifers. From this amount, use 100 g/m³ as background feeding. 1300
40
Never exceed a maximum daily feeding of 600ppm. 1200
38

1100

%/day
36

DATA 1000 34

900 32

30
800
Clean Average
Average ± St.Error: 28
Mean
Mean±SE
700 most probable growth rates
Min-Max
Min-Max 26 Outliers
S.parkle has an optimal behaviour both during preparation and in the culture tank. The surface is clean, without foam or flock formation, for a 600 Extremes
24
constant rotifer performance. Standard culture diet S.parkle Standard Culture Diet S.parkle

* Specific Growth Rate (SGR): specific daily growth expressed in % (relative value)

3 day batch culture 3 day batch culture

Start density: 750 rotifers/ml Start density: 1.000 rotifers/ml

45 30,0 40 25

40 35
25,0
20
35
30

30 20,0
25
15

% eggs day 3

% eggs day3
SGR (%/day)

SGR(%/day)
25
15,0 20
20
10
15
15 10,0

10
10
5
5,0
5 5

0 0,0 0 0
RUN1 RUN2 RUN3 RUN4 RUN1 RUN2 RUN5 RUN6