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FORENSIC GENETIC APPROACHES
FOR IDENTIFICATION OF HUMAN
SKELETAL REMAINS
FORENSIC GENETIC
APPROACHES FOR
IDENTIFICATION OF
HUMAN SKELETAL
REMAINS
Challenges, Best Practices, and
Emerging Technologies
Edited by
v
In Memory of:
Paula C. Brumit, DDS, D-ABFO
(1956–2021)
This book honors the memory and legacy of sisted in the identification of victims from
Dr. Paula Brumit—an exceptional scientist, Hurricane Katrina and the 9/11 World Trade
humanitarian, mentor, and friend. She left an Center terrorist attacks); forensic odontolo-
indelible mark on not just the forensic human gist for the Bexar County Medical Examiner’s
identification community but also on all who Office (Bexar County, Texas); and consultant
were fortunate enough to have crossed her for the FBI, the Texas Rangers, Immigration
path personally or professionally. Her gener- and Customs Enforcement (ICE), Child
ous spirit, kindness, altruism, selflessness, and Protective Services (CPS), Department of
willingness to share knowledge will never be Public Safety (DPS), and various law en-
forgotten. She was the best friend one could forcement agencies.
ever hope for, and her sense of humor was Dr. Brumit was a member of the American
unmatched—she could make you laugh in
Society of Forensic Odontology (ASFO) and
even the most despondent of times. I was for- served on the ASFO’s Board of Governors.
tunate to have the gift of her friendship, and I She was a fellow of the American Academy
will miss her every day for the rest of my life. of Forensic Sciences (AAFS) and served as
Dr. Brumit was remarkably accomplished, Secretary, Program Chair, and Odontology
immensely respected by her peers, and de- Section Chair as well as on the AAFS Board
voted her life in service to others. She earned of Directors. Dr. Brumit was only the second
a Bachelor of Science degree in dental hygiene woman to hold the office of Odontology
and a Doctor of Dental Surgery (DDS) degree Section Chair in the 71-year history of the
from Texas A&M Health Science Center’s American Academy of Forensic Sciences.
Baylor College of Dentistry. During her ca- In addition, she was a Diplomate of the
reer, she made innumerable contributions to American Board of Forensic Odontology
forensic odontology and human identifica- (ABFO), served two consecutive terms on
tion casework in various professional roles, the ABFO Board of Directors, and one term
including as consulting forensic odontologist as ABFO President. During her career, she
for the Dallas County Medical Examiner's was appointed to the Texas Forensic Science
Office (Dallas, Texas); consultant for the Commission’s Bitemark Case Review
UNT Center for Human Identification’s Team, the National Institute of Standards
Laboratory of Forensic Anthropology (Fort and Technology (NIST) Scientific Area
Worth, Texas); member of the National Committees (SACs) Forensic Odontology
Disaster Mortuary Operational Response Subcommittee, and the Criminal Justice
Team (DMORT) Region 6 (in which she as- Information Services (CJIS) National Dental
vii
viii In Memory of Paula C. Brumit, DDS, D-ABFO (1956–2021)
Image Repository Review Panel for the Fellowship program and in the Forensic
Federal Bureau of Investigation (FBI). Dentistry master’s program. Dr. Brumit
In 2020, she was elected to the Forensic had recently been promoted to Associate
Specialties Accreditation Board for a 4-year Professor in the College of Dental Medicine
term and was hired by the National Center at Lincoln Memorial University (LMU),
for Missing & Exploited Children (NCMEC) and was hired as a forensic odontologist for
to assist in the fight against the abuse and ex- LMU’s Institute for Human Identification.
ploitation of children. In addition, she was Co-Director of
Academically, Dr. Brumit held an ap- the Southwest Symposium on Forensic
pointment as Assistant Professor in the Dentistry, and for nearly two decades was
Department of General Dentistry (Division an adjunct assistant professor in the Forensic
of Forensic Odontology) at the University Fellowship Program within the Center for
of Tennessee Graduate School of Medicine Education and Research in Forensics (CERF)
(UTGSM) in Knoxville, Tennessee. She at the University of Texas Health Science
taught in both UTGSM’s Forensic Odontology Center (San Antonio, Texas).
Contributors
Angie Ambers PhD Henry C. Lee Insti- Bobby L. LaRue, PhD Department of Forensic
tute of Forensic Science; Forensic Science Science, Sam Houston State University, Hunts-
Department, Henry C. Lee College of Crim- ville, TX, United States; Verogen Inc., San
inal Justice and Forensic Sciences, Center Diego, CA, United States
for Forensic Investigation of Trafficking in Brandon Letts PhD Federal Bureau of Investi-
Persons, University of New Haven, West gation (FBI) Laboratory, Quantico, VA, United
Haven, CT; Institute for Human Identifica- States
tion, LMU College of Dental Medicine, Knox-
ville, TN, United States James M. Lewis DMD, D-ABFO Department
of General Dentistry, Division of Forensics,
Eric J. Bartelink PhD, D-ABFA Department University of Tennessee Graduate School of
of Anthropology, Human Identification Labo- Medicine; Institute for Human Identification,
ratory, California State University, Chico, CA, LMU College of Dental Medicine, Knoxville,
United States TN; Alabama Department of Forensic Sciences,
Howard D. Cash CEO Gene Codes Forensics, Madison, AL, United States
Inc., Ann Arbor, MI, United States
Odile Loreille PhD Federal Bureau of Investi-
Thomas J. David DDS, D-ABFO Georgia gation (FBI) Laboratory, Quantico, VA, United
Bureau of Investigation, Division of Forensic States
Sciences, Decatur, GA; Department of General
Murray K. Marks, PhD, D-ABFA Knox County
Dentistry, Division of Forensics, University of
Regional Forensic Center, Department of
Tennessee Graduate School of Medicine; Insti-
General Dentistry, Division of Forensic Odontol-
tute for Human Identification, LMU College
ogy, University of Tennessee Graduate School of
of Dental Medicine, Knoxville, TN, United
Medicine; National Forensic Academy Outdoor
States
Decomposition Training Facility, University of
Suni M. Edson PhD Assistant Technical Leader, Tennessee; Department of Pathology; Institute
Past Accounting Section, Armed Forces DNA for Human Identification, LMU College of
Identification Laboratory (AFDIL), Armed Dental Medicine, Knoxville, TN, United States
Forces Medical Examiner System, Dover, DE,
Timothy P. McMahon PhD Department of
United States
Defense (DoD) DNA Operations, Armed Forces
Colleen Fitzpatrick PhD Identifinders Interna- Medical Examiner System, Defense Health
tional LLC, Fountain Valley, CA, United States Agency, Dover, DE, United States
Ranjana Grover PhD ANDE Corporation, Darinka Mileusnic-Polchan MD, PhD,
Waltham, MA, United States D-ABP Knox County Regional Forensic
Michael Hennessey BGS, MBA Human Iden- Center, Department of Pathology, University of
tification Projects, Gene Codes Forensics, Inc., Tennessee Graduate School of Medicine; Insti-
Ann Arbor, MI, United States tute for Human Identification, LMU College of
Irene Kahline MSFS Past Accounting Section, Dental Medicine, Knoxville, TN, United States
Armed Forces DNA Identification Laboratory Stephen Missal CFA Maricopa County Medical
(AFDIL), Armed Forces Medical Examiner Examiner Office, Forensic Science Center; Art
System, Dover, DE, United States Institute of Phoenix, Phoenix, AZ, United States
xv
xvi Contributors
Nicole Novroski PhD Department of Anthro- Jodie Ward PhD Centre for Forensic Science,
pology, Forensic Science Program, University University of Technology Sydney, Sydney,
of Toronto Mississauga, Toronto, ON, Canada NSW, Australia
Vivek Sahajpal, PhD State Forensic Science Jeremy Watherston PhD Forensic and Analyt-
Laboratory, Directorate of Forensics Services, ical Science Service, NSW Health Pathology,
Shimla, Himachal Pradesh, India Lidcombe, NSW, Australia
Richard F. Selden MD, PhD ANDE Corpora- Frank R. Wendt PhD Department of Psychiatry,
tion, Waltham, MA, United States Yale School of Medicine, U.S. Department of
Eugene Tan PhD ANDE Corporation, Waltham, Veteran Affairs Connecticut Healthcare System,
MA, United States New Haven, CT, United States
Rosemary Turingan Witkowski PhD ANDE
Corporation, Waltham, MA, United States
Author’s biographies
Angie Ambers, PhD DNA topics. During this time, she also
Dr. Angie Ambers is an internationally was a team member with UNH’s Center for
recognized DNA expert and Director of Forensic Investigation of Trafficking in Persons.
Forensic Genetics at the Institute for Human She worked as a forensic geneticist for the
Identification in the College of Dental Medicine University of North Texas (UNT) Center for
at Lincoln Memorial University (LMU). She Human Identification for 8 years and, prior
also is a Professor of Forensic Genetics in the to pursuing her doctorate, was lead DNA
“Forensic Human Identification” graduate analyst and lab manager of UNT’s DNA
program. She has a PhD in molecular biol- Sequencing Core Facility. Her doctoral re-
ogy (with an emphasis in forensic genetics search involved an investigation of methods
and human identification) as well as mas- (e.g., whole-genome amplification, DNA
ter’s degrees both in forensic genetics and repair) for improving autosomal and Y-STR
criminology. Dr. Ambers conducts research typing of degraded and low copy number
at the National Forensic Academy (NFA) (LCN) DNA from human skeletal remains
Cumberland Forest Outdoor Decomposition and environmentally damaged biological
Facility, a research and training center de- materials. Her master’s thesis research in-
voted to studying the effects of environmental cluded the development and optimization
exposure on postmortem human remains. In of a DNA-based multiplex screening tool
collaboration with a team of board-certified for genetics-based separation of fragmented
forensic anthropologists and odontologists, and commingled skeletal remains in mass
her research mission is to develop and op- graves.
timize methodologies to improve identifi- Dr. Ambers specializes in the character-
cation efforts in missing person cases, mass ization and identification of contemporary,
disasters, and unidentified human remains historical, and archaeological human skel-
(UHR) investigations. etal remains. Her casework has involved
Prior to LMU, Dr. Ambers previously DNA testing of an American Civil War guer-
served as Assistant Director of the Henry C. rilla scout; several Finnish World War II sol-
Lee Institute of Forensic Science in Connecticut, diers; unidentified late-19-century skeletal
a world-class institute that specializes in in- remains discovered by a construction crew
terdisciplinary research, training, testing, in Deadwood, South Dakota; unidentified
casework consulting, and education in fo- skeletal remains of Special Operations sol-
rensic science. She also held an Associate diers killed during the 1974 Turkish invasion
Professor (Forensic DNA) appointment in of Cyprus; skeletal remains exhumed from
the Henry C. Lee College of Criminal Justice Prague Castle in the Czech Republic; skele-
and Forensic Sciences at the University of tal remains of soldiers from the seven years’
New Haven (UNH), teaching forensic bi- war (1756–63); bone samples purported
ology and DNA analysis methods, in ad- to belong to a member of Jesse James gang
dition to specialty courses on advanced (killed during a bank robbery in 1876); and
xvii
xviii Author’s biographies
the exhumed remains of the wife of a Yale grave at Fort St. Louis, and the p utative
medical school professor. She co-presented remains of Sieur de Marle. Additionally,
a workshop on “Advanced Methods for Dr. Ambers performed DNA extractions and
DNA-based Analysis of Skeletal Remains” mitochondrial DNA (mtDNA) sequencing
at the 26th International Symposium on on numerous sets of skeletal remains asso-
Human Identification (ISHI), and has been ciated with Spanish royalty and the House
an invited speaker at an international bone of Aragon (recovered from tombs within
workshop/conference in Prague, Czech the Royal Pantheon of San Juan de la Peña
Republic. In 2017, she traveled twice to India archaeological site in Spain). She is currently
to train scientists from various Indian states working on the putative remains of William
and the Maldives Police Service on the pro- Townsend Washington, the nephew of for-
cessing of bone samples in forensic DNA mer U.S. President George Washington.
casework. During her visit to India, she per- In addition to skeletal remains cases and
formed autosomal STR analysis on human research, Dr. Ambers collaborated with the
skeletal remains discovered along a hiking National Institute of Justice (NIJ) and the
route in the Himalayas (in the northern state Forensic Technology Center of Excellence
of Himachal Pradesh) to assist local officials (FTCoE) to develop and disseminate a formal
in the investigation of a missing person case. report on the use of familial DNA searching
Additionally, she performed DNA analysis (FDS) in casework. She is an active cold case
on a female homicide victim recovered from consultant, an advocate of postconviction
a clandestine grave in New Delhi. DNA testing, and an educator/advisor on
Dr. Ambers’ casework and research DNA testing or retesting of old, degraded, or
have been published in various peer- challenging evidentiary samples. Dr. Ambers
reviewed journals, including Forensic Science also was the 2017–18 Project Lead on a
International: Genetics (FSI: Genetics), Forensic U.S. State Department grant to combat hu-
Science International (FSI), International Journal man trafficking in Central America through
of Legal Medicine (IJLM), Legal Medicine, BMC the application of forensics. As part of the
Genomics, the Croatian Medical Journal, The program objectives, she traveled to three
Journal of Heredity, and Journal of Biological Northern Triangle countries (Guatemala, El
and Clinical Anthropology (Anthropologischer Salvador, Honduras) to perform gap assess-
Anzeiger). Her work has received press in ments of government laboratories and train
numerous local and national newspapers personnel in forensic DNA analysis, with the
(including The Washington Times, NBC News, goal of promoting quality casework methods
Criminal Legal News, The Root) and has been based on ISO 17025 standards. As Project
featured on several podcasts (Truth and Lead, she helped in training forensic DNA
Justice, Crime Waves). scientists at the Instituto Nacional de Ciencias
Her most recent casework (a collabora- Forenses de Guatemala (INACIF, the National
tion with the Texas Historical Commission) Institute of Forensic Sciences of Guatemala)
includes DNA analyses of human skele- in Guatemala City, Guatemala; the Instituto
tal remains associated with the French ex- de Medicina Legal (Institute of Legal Medicine)
plorer La Salle’s last expedition. This case in San Salvador, El Salvador; and the Instituto
involved massively parallel sequencing de Medicina Forenses: Centro de Medicina Legal
(MPS) of DNA from two adult male skele- y Ciencias Forenses (the Institute of Forensic
tons recovered from the La Belle shipwreck, Medicine: Center of Legal Medicine and
skeletal remains recovered from a mass Forensic Sciences) in Tegucigalpa, Honduras.
Author’s biographies xix
In addition to providing both lecture and Anthropology 10e (Cengage), Introduction to
tactile training on DNA analysis methods to Physical Anthropology 15e (Cengage), and a co-
Northern Triangle laboratories, Dr. Ambers editor on New Perspectives in Forensic Human
was part of a consortium to help these coun- Skeletal Identification (Elsevier). Dr. Bartelink
tries develop and maintain forensic DNA teaches courses in introductory physical
databases to assist in the identification of anthropology, as well as forensic science,
missing persons related to human trafficking. human osteology, forensic anthropology, bio-
archaeology, and statistics. He is a Fellow of
Eric Bartelink, PhD, D-ABFA the American Academy of Forensic Sciences
Dr. Eric J. Bartelink is a Professor in (AAFS), and a member of the American
the Department of Anthropology and Association of Physical Anthropologists
Co-Director of the Human Identification (AAPA), Society of American Archaeology
Laboratory at California State University, (SAA), Paleopathology Association (PPA), and
Chico. He received his BS in Anthropology at the Society for California Archaeology (SCA).
Central Michigan University (1995), his MA in He is a past Board Member and President of
Anthropology at California State University, the American Board of Forensic Anthropology
Chico (2001), and his PhD in Anthropology (ABFA), and former Board Member of the
at Texas A&M University (2006). He is a American Academy of Forensic Sciences
board-certified Diplomate of the American (AAFS), the Scientific Working Group for
Board of Forensic Anthropology (ABFA). Forensic Anthropology (SWGANTH), and the
Dr. Bartelink’s interests are in forensic Organization of Scientific Area Committees
anthropology and bioarchaeology, and he (OSAC) Anthropology Subcommittee.
has conducted research focused on skel-
etal trauma, taphonomy, paleopathology, Howard Cash, CEO
and stable isotope analysis. He maintains Howard Cash is the CEO of Gene Codes
an extensive research program focused on Corporation. Before venturing into the
central California bioarchaeology and also field of computational biology, he studied
has conducted work in American Samoa. music at the University of Pennsylvania
Previously, he assisted with the excavation of and also served as Assistant Conductor
mass graves in Bosnia-Herzegovina through with the Pennsylvania Opera Theater,
the United Nations International Criminal Psychoacoustics at Stanford.
Tribunal for the Former Yugoslavia, and also At the forefront of commercial bioinfor-
assisted in the identification of victims from matics since 1984, Cash was Senior Engineer
the World Trade Center 9/11 disaster. and headed the Expert Systems Group at
He has published articles in Journal of IntelliGenetics, Inc., where many seminal
Forensic Sciences, Forensic Science International, biotechnology software tools were devel-
Forensic Sciences Research, American Journal of oped. One of the programs he worked on
Physical Anthropology, Journal of Archaeological during this period was key to the original
Science, International Journal of Osteoarchaeology, sequencing of the human immunodeficiency
International Journal of Paleopathology, Journal of (HIV/AIDS) virus. In 1988, he founded Gene
Archaeological Method and Theory, Archaeometry, Codes Corporation, one of the most success-
and California Archaeology. He is also a ful bioinformatics companies in the world.
co-author on the books Forensic Anthropology: He designed and developed the Sequencher
Current Methods and Practice (Elsevier, 1st program used in thousands of academic and
and 2nd editions), Essentials of Physical commercial DNA sequencing labs in more
xx Author’s biographies
and New Zealand. She has appeared in hun- in New York City and provided opera-
dreds of newspapers and magazines (both tional consulting expertise on the World
domestically and internationally), and on Trade Center identification project. In 2005,
international radio and television programs. Mr. Hennessey also served on the DNA
She is the author of three books: Forensic Subcommittee of the Thailand Tsunami
Genealogy, DNA & Genealogy, and The Dead Victim Identification Center in Phuket,
Horse Investigation: Forensic Photo Analysis for Thailand. He has helped lead Mass Fatality
Everyone. Identification System (M-FISys) customer
training for users in Guatemala, Kenya,
Ranjana Grover, PhD El Salvador, Costa Rica, Libya, Israel,
Dr. Ranjana Grover is Vice President of Mexico, and Saudi Arabia. Additionally,
Molecular Biology at ANDE Corporation. Mr. Hennessey has conducted human iden-
She received a BS in Zoology (summa cum tification workshops at national and in-
laude), an MS in Genetics, and a PhD in ternational forensics meetings, published
Molecular Human Genetics from Delhi peer-reviewed journal articles, and serves on
University. She is an expert in the field of the Scientific Advisory Board of the Forensic
developing rapid, highly multiplexed PCR Anthropology Foundation of Guatemala
assays for diverse applications ranging from (FAFG). He has a Master’s of Business
human identification to in vitro diagnostic Administration (MBA) from the University
assays for human diseases. Dr. Grover has of Michigan with a concentration in opera-
worked in a high-throughput human genetics tions management.
research laboratory exploring the genetics of
complex traits and has a strong background Irene Kahline, MS
in statistical methods related to molecular Irene Liunoras Kahline received her
genetics. She received postdoctoral train- Bachelor of Science (BS) in Biochemistry
ing at Johns Hopkins University and then at Florida State University. Following her
joined the Henry M. Jackson Foundation, undergraduate studies, she worked in a re-
where she worked on identifying genetic search lab studying the evolution and phy-
and epigenetic markers related to Down syn- logeny of shark and ray DNA. During her
drome, traumatic brain injury, and the toxic time in the research lab, she decided to pur-
effects of metals employed in bullets. While sue a career in forensic DNA. She moved
working on these projects, she received sev- to the Washington, D.C. area to obtain
eral Department of Defense (DoD) research her Master of Forensic Science in Forensic
grants and developed expertise in highly Molecular Biology at George Washington
advanced next-generation sequencing (NGS) University. While pursuing her master’s de-
technologies and in second- generation se- gree, she interned with the research depart-
quencing analysis. ment (now Emerging Technology section)
at the Armed Forces DNA Identification
Michael Hennessey, MBA Laboratory (AFDIL).
Mr. Hennessey was the onsite Project Irene has steadily moved through differ-
Manager for Gene Codes Forensics (GCF) ent positions at AFDIL from research intern
during the identification of victims of the to DNA technician to DNA analyst, and
9/11 terrorist attacks in 2001. For 3 years now as a supervisory DNA analyst for the
after the attacks, he reported daily to the Past Accounting Section. She is proficient
Office of Chief Medical Examiner (OCME) in mitochondrial DNA (mtDNA) Sanger
Author’s biographies xxiii
sequencing, next-generation sequencing vironmental changes on animal populations.
(NGS) of whole-genome mitochondrial DNA, His research interests continue to revolve
autosomal STR typing, and Y-STR technolo- around the retrieval and analysis of DNA
gies working on dried skeletal remains from from highly degraded samples.
past military conflicts. Her dedication to the
mission of AFDIL was so immense that she James M. Lewis, DMD, D-ABFO
agreed to move to Dover, Delaware from the Dr. James M. Lewis earned his dental
Rockville, Maryland location. degree from the University of Alabama
School of Dentistry (Birmingham) in 1985.
Bobby LaRue, PhD He maintained a general dentistry practice
Dr. Bobby LaRue earned a PhD in Molecular exclusively in Madison, Alabama from 1986
Genetics from the University of North to 2018. Dr. Lewis completed a fellowship
Texas (UNT) and completed an Intelligence in Forensic Odontology from the Center
Community postdoctoral fellowship in which for Education and Research in Forensics
he studied the feasibility of small-amplicon, at the University of Texas Health Science
di-allelic markers for forensic applications. Center at San Antonio in 2001. As a board-
Dr. LaRue recently left his position as an certified forensic odontologist, he assisted
Associate Professor of Forensic Science at in victim identification following the World
Sam Houston State University (SHSU) to Trade Center attack in New York City and,
accept a position in private industry (with since 2003, has served as a consultant to the
Verogen, Inc.). Alabama Department of Forensic Sciences
(ADFS) and a volunteer to the Alabama
Brandon Letts, PhD Office of Emergency Preparedness in relation
Dr. Brandon Letts is a Research Biologist to its mass disaster response group.
at the FBI Laboratory in Quantico, Virginia. Dr. Lewis is a Fellow of the Odontology
He possesses a BS in Forensic Science from Section of the American Academy of
Baylor University and a PhD in Genetics Forensic Sciences (AAFS), became board-
from Pennsylvania State University. His cur- certified by the American Board of Forensic
rent duties include research and validation Odontology (ABFO) in 2008, has served on
of new techniques and technologies for im- the Board of Governors of the American
plementation at the FBI and troubleshooting Society of Forensic Odontology (ASFO),
laboratory issues arising in casework. Prior and became President of the organization
to becoming a research biologist, he was a fo- in 2012. For the American Board of Forensic
rensic examiner for the U.S. Army Criminal Odontology (ABFO), he served as member
Investigation Laboratory (USACIL) and both and Chair of the Dental Age Assessment
a forensic examiner and technical specialist Committee (2008–20); as a member of the
for the FBI. Certification and Examination Committee
Dr. Letts has a background in ancient (2011–15); Bitemark Evidence and Patterned
DNA, having performed his dissertation Injury Committee (2008–15; 2017–19); and
work under the supervision of Dr. Beth currently holds the office of ABFO President.
Shapiro, a leader in the study of ancient He is currently appointed to the Odontology
DNA and molecular evolution. In her lab, Subcommittee of the Organization of
he analyzed hundreds of samples up to Scientific Area Committees (OSAC) for
100,000 years old and used the information Forensic Science with the National Institute
he obtained to investigate the effects of en- of Standards and Technology (NIST).
xxiv Author’s biographies
Dr. Lewis previously held the position retrieval of nuclear DNA from decades-old
of Director of Forensic Dentistry (Division human rootless hair shafts and characteri-
of Forensics) in the Department of General zation of single nucleotide polymorphisms
Dentistry at the University of Tennessee (SNPs) to identify the hair’s owner. Another
Graduate School of Medicine. He is currently research interest of hers is DNA analysis
Director of Forensic Odontology at the Institute of remains attributed to historical figures.
for Human Identification in the College of Dental These projects involved the tentative identi-
Medicine at Lincoln Memorial University fication of W.A Mozart, Friedrich Schiller, the
(LMU). He is also a professor of Forensic unknown child of the Titanic, Everett Ruess,
Dentistry at LMU and an instructor for the The Romanov family (including the two chil-
National Forensic Academy (NFA) in Oak dren found in 2007), Sir George Yeardley, and
Ridge, Tennessee. He has edited numerous Henry Rathbone.
textbooks, authored textbook chapters, and Dr. Loreille has been a member of the
published a variety of articles in peer-reviewed International Society for Forensic Genetics
journals on forensic odontology. (ISFG) for the past 13 years and has pub-
lished 30 peer-reviewed research articles
Odile Loreille, PhD or book chapters. Her 2007 publication on
Dr. Odile Loreille holds a PhD in DNA extraction using total demineralization
Paleogenetics from the University of Claude has been cited more than 250 times, and her
Bernard in Lyon, France. During her PhD 2018 research article on the DNA analysis of
research and two postdoctoral positions, a 4000-year-old Egyptian mummy is one of
she specialized in extracting and analyzing the most-read articles of the journal.
DNA from extinct species (cave bears, cave
lions), mummies, and decades-old para- Murray K. Marks, PhD, D-ABFA
sites. When she joined the Armed Forces Dr. Murray Marks is the board-certified fo-
DNA Identification Laboratory (AFDIL) rensic anthropologist for the Regional Forensic
in 2004, she used her expertise in ancient Center (RFC) in Knoxville, Tennessee, and for
DNA (aDNA) analysis to tackle AFDIL’s the Hamilton County Medical Examiner’s
most challenging identification effort—the Office in Chattanooga. In these respective ap-
so-called Punchbowl samples, a large set pointments, he performs recovery, biological
of Korean War remains buried as unknown assessment, skeletal trauma, and identification
in the National Memorial Cemetery of the casework on human remains. Dr. Marks also
Pacific. After optimizing the DNA extraction serves as Director of Forensic Anthropology
protocol with a technique that is now used in at the Institute for Human Identification in
many forensic laboratories around the world, the College of Dental Medicine at Lincoln
she introduced next-generation sequenc- Memorial University (LMU). Additionally,
ing (NGS) to the laboratory. Using Illumina he is a Professor of Forensic Anthropology
technology and hybridization capture, she and Human Identification at LMU and man-
successfully retrieved mitochondrial DNA ages the National Forensic Academy (NFA)
(mtDNA) from these human remains. Outdoor Decomposition Training Facility, where
Dr. Loreille is now a Research Biologist in he teaches archaeological clandestine grave
the DNA Support Unit at the FBI Laboratory recovery methods and forensic anthropol-
and continues to work on improving tech- ogy. Dr. Marks previously held an Associate
niques for the FBI’s DNA Casework Unit. Research Professor appointment in the
The focus of her current research is the Graduate School of Medicine’s Department
Author’s biographies xxv
of General Dentistry (Division of Forensics) Line that allowed for the processing of 640
at the University of Tennessee Medical Center crime scene samples per day, developed an
Knoxville (UTMCK), instructing students in automated differential extraction line, and
the M.S. program on forensic dentistry, head performed over 200 validations for both
and neck anatomy, mineralized tissue his- North American and international crime lab-
tology, and research methods. He currently oratories. In February 2012, Dr. McMahon
holds adjunct visiting professorships in the returned to the Armed Forces Medical
Department of Anatomy and Neurobiology Examiner System (AFMES) as the Chief of
at Boston University and in the Department Forensic Services, where his role as the sci-
of Anatomy at the University of Western entific subject matter expert was to advise,
Australia. guide, and help maintain the AFMES as a
leader in the forensic community. He over-
Timothy McMahon, PhD saw all aspects of casework performed at
Dr. Timothy McMahon received his PhD DoD DNA operations while directing and
in Biomedical Sciences from the School of carrying out projects and programs to de-
Public Health at the University of Albany, velop innovative advances in DNA-based
New York. Dr. McMahon’s graduate stud- forensic testing methods. In this role, Dr.
ies and postdoctoral research were per- McMahon also established AFMES-AFDIL
formed in the division of Infectious Disease as the first laboratory in the United States to
and Immunology at the New York State develop and implement a forensically val-
Department of Health. His research included idated next-generation sequencing (NGS)
the identification and interaction of the method for human remains identification.
Human Cytomegalovirus Helicase-Primase In 2017, Dr. McMahon became the first ci-
replication proteins and a pro-death late vilian Director of the DoD DNA operations
transcript that caused cell death in infected section of the AFMES, where he is in charge
cells. From 2002 to 2007, Dr. McMahon es- of the Armed Forces DNA Identification
tablished the U.S. Department of Defense Laboratory (AFDIL) and the Armed Forces
(DoD) DNA Operation’s Quality Control Repository of Specimen Samples for the
and Validation section; in this role, he was Identification of Remains. Dr. McMahon
responsible for developing and forensically oversees over 150 scientists and is in charge
validating all new human remains DNA of all human remains DNA scientific initia-
identification instrumentation, reagents, and tives in support of the AFMES’ primary mis-
testing procedures. sion to identify fallen U.S. Service members
In March 2007, Dr. McMahon joined from present-day operations and from past
Applied Biosystems, where he established conflicts.
the first commercial Global DNA Forensic
Professional Service organization whose Darinka Mileusnic-Polchan, MD, PhD,
primary function was to design and estab- D-ABP
lish new DNA laboratories (as well as aid Dr. Darinka Mileusnic-Polchan is a grad-
local, state, and federal crime laboratories uate of the University of Rijeka Medical
with implementing new forensic DNA tech- School in Croatia. After earning her med-
nologies and instrumentation). During his ical degree, she graduated from Loyola
time at Applied Biosystems, Dr. McMahon University’s Neuroscience Graduate
was responsible for developing a state of Program in Chicago, Illinois. She com-
the art, fully automated Criminal Casework pleted her training in anatomic pathology
xxvi Author’s biographies
in the Department of Pathology, Loyola Arizona counties, has worked with law en-
University Stritch School of Medicine. forcement in Georgia, and is certified by the
Dr. Mileusnic completed forensic pathol- International Association for Identification
ogy training at the Cook County Office of (IAI). Stephen actively pursues a recon-
the Medical Examiner in Chicago, Illinois, struction caseload and contributes to edu-
subsequently receiving board certification cating the public about forensic art through
in Anatomic and Forensic Pathology. Upon lectures at various colleges and to high
completion, she accepted the position of school audiences. As an educator, Stephen
Assistant Medical Examiner for the Cook achieved full Professor at the Art Institute of
County Office of the Medical Examiner for Phoenix, where he taught with an elite fac-
several years. ulty that included two Emmy winners and
In 2002, Dr. Mileusnic-Polchan relocated several nationally recognized illustrators
to Knoxville, Tennessee, where she continued and designers. In 2016, he worked with the
the same line of forensic casework in the NRK (the national Norwegian broadcasting
Department of Pathology at The University network) as the forensic artist responsible
of Tennessee Graduate School of Medicine for a new reconstruction of the Isdal Woman,
(UTMCK). Currently, she is a Clinical Associate which was included in the documentary
Professor of Pathology at UTMCK. In 2008, about the case which aired that same year.
she accepted the position of Chief Medical Stephen’s facial reconstruction for this case
Examiner for Anderson County and Knox has since appeared in Die Zeit and many
County in the state of Tennessee. Dr. Mileusnic other European news outlets over the past
is the Medical Director of the Knox County 4 years. Aside from his forensic casework,
Regional Forensic Center (RFC), which has Stephen’s paintings and drawings can be
been accredited by the National Association of found in hundreds of private collections na-
Medical Examiners since 2010. She chaired the tionally. He also has co-authored and illus-
State Medical Examiner Advisory Council and trated two college textbooks on drawing for
has been an invited speaker at numerous local animation, and has appeared on local and
and national conferences. national cable television demonstrating his
forensic art techniques.
Stephen Missal, MFA, CFA Scores of Stephen’s forensic reconstruc-
Stephen Missal graduated with a BFA tions can be found in the National Missing
and MFA in painting from Wichita State and Unidentified Persons System (NamUs),
University and taught art at the college as well as on the Maricopa County Medical
level for over four decades. He later re- Examiner’s site where unknown cases are
ceived his training in forensic art by available for public view.
world-renowned forensic artists Karen T.
Taylor and Betty Pat Gatliff. Stephen has Nicole Novroski, PhD
specialized in forensic facial reconstruc- Dr. Nicole Novroski is a forensic geneti-
tion, and for the past 12 years, he has been cist and tenure-stream faculty member at the
the sole forensic facial reconstruction art- University of Toronto in the Department of
ist working with the Maricopa County Anthropology, with a primary teaching ap-
Medical Examiner in Arizona (home to pointment in the Forensic Science Program
Phoenix, Mesa, Scottsdale, and a number at the University of Toronto Mississauga
of other large cities). In addition, he does (UTM). Her research program focuses on
forensic reconstructions for several other novel and innovative approaches to improve
Author’s biographies xxvii
upon current forensic genetic methodologies Within her laboratory, she has a growing team
using traditional (PCR-CE) and MPS-based of undergraduate and graduate trainees.
instrumentation. Further, Dr. Novroski assists legal profession-
Dr. Novroski’s academic accolades in- als and the public as a forensic DNA expert
clude a Bachelor of Science (Hons) in through her firm 4NGen Consulting. In her
Forensic Science and Biology from the limited spare time, she volunteers with the
University of Toronto, a Master of Science Junior League of Toronto and Toronto Counter
in Forensic Biology from the University at Human Trafficking Network and is actively
Albany (SUNY), and a Doctor of Philosophy pursuing a degree in Forensic Accounting to
in Molecular Genetics from the University further diversify her portfolio.
of North Texas Health Science Center
(UNTHSC) at Fort Worth, Texas. Her edu- Vivek Sahajpal, PhD
cational training is largely focused on the Dr. Vivek Sahajpal is Assistant Director
exploration of previously uncharacterized of the Directorate of Forensics Services in
genetic markers for improved DNA mixture Himachal Pradesh, India. In addition, he
de-convolution of complex forensic profiles. is the Nodal Officer for the Disaster Victim
Dr. Novroski is the recipient of numerous Identification (DVI) Cell at the Directorate of
awards including the Forensic Sciences Forensic Services. Dr. Sahajpal has performed
Foundation (AAFS) Douglas M. Lucas Grant, forensic DNA analyses in more than 2000
the Connaught New Researcher Award, and criminal cases, as well as for numerous cases
the University of Toronto Mississauga Dean’s involving the identification of human skele-
Award for Excellence (Faculty). She is an as- tal remains and disaster victim identification.
sociate member of the American Academy Dr. Sahajpal attained his master’s degree in
of Forensic Sciences (AAFS), an active mem- Forensic Science from Punjabi University
ber of the International Society of Forensic Patiala, and his concern for wildlife con-
Genetics (ISFG), and an active member of servation and his interest in the field of
the Scientific Research Honor Society Sigma Wildlife Forensics brought him to the
Xi. Her publication record includes over 20 Wildlife Institute of India, Dehradun. At
peer-reviewed manuscripts, 35 scientific the Wildlife Institute of India, Dr. Sahajpal
communications, public abstracts, and oral carried out some of the pioneering research
presentations as well as two book chapters. work in the field of Wildlife Forensics and
She is an ad hoc reviewer for multiple inter- genetics. He earned his PhD in Forensic
national journals in the areas of human and Science from Punjabi University, Patiala.
forensic genetics and serves as a committee Identification of human skeletal remains
member for LabRoots Forensic Science, the has been an important area of work for
Swiss National Science Foundation, and the Dr. Sahajpal because Himachal Pradesh
American Society of Human Genetics “DNA is a popular tourist destination for hikers,
Day” Essay Contest. trekkers, and pilgrims—who often go miss-
Dr. Novroski is passionate about mentor- ing at high altitudes in the Himalayas (and
ing, volunteerism, and continuing education. along mountain passes and rivers) after get-
She actively holds mentorship positions with ting lost or due to abrupt weather changes.
the University of Toronto Mississauga Alumni Skeletonized remains are quite often recov-
Mentor Program as well as the Women in ered in these cases.
Science Mentorship Program (as part of an Dr. Sahajpal is currently working on the
ongoing NSERC CREATE PROMOTE Grant). genetic diversity of different forensically
xxviii Author’s biographies
xxxiii
xxxiv Editor biography
remains of soldiers from the 7 Years’ War (1756–63); bone samples purported to belong to a
member of Jesse James gang (killed during a bank robbery in 1876); and the exhumed remains
of the wife of a Yale medical school professor. She copresented a workshop on “Advanced
Methods for DNA-based Analysis of Skeletal Remains” at the 26th International Symposium
on Human Identification (ISHI), and has been an invited speaker at an international bone work-
shop/conference in Prague, Czech Republic. In 2017, she traveled twice to India to train sci-
entists from various Indian states and the Maldives Police Service on the processing of bone
samples in forensic DNA casework. During her visit to India, she performed autosomal STR
analysis on human skeletal remains discovered along a hiking route in the Himalayas (in the
northern state of Himachal Pradesh) to assist local officials in the investigation of a missing
person case. Additionally, she performed DNA analysis on a female homicide victim recovered
from a clandestine grave in New Delhi.
Dr. Ambers’ casework and research have been published in various peer-reviewed journals,
including Forensic Science International: Genetics (FSI:Genetics), Forensic Science International,
International Journal of Legal Medicine, Legal Medicine, BMC Genomics, the Croatian Medical Journal,
The Journal of Heredity, and Journal of Biological and Clinical Anthropology (Anthropologischer
Anzeiger). Her work has received press in numerous local and national newspapers (including
The Washington Times, NBC News, Criminal Legal News, The Root) and has been featured on sev-
eral podcasts (Truth and Justice, Crime Waves).
Among her most recent casework (a collaboration with the Texas Historical Commission)
includes DNA analyses of human skeletal remains associated with the French explorer La
Salle’s last expedition. This case involved massively parallel sequencing (MPS) of DNA
from two adult male skeletons recovered from the La Belle shipwreck, skeletal remains
recovered from a mass grave at Fort St. Louis, and the putative remains of Sieur de Marle.
Additionally, Dr. Ambers performed DNA extractions and mitochondrial DNA (mtDNA)
sequencing on numerous sets of skeletal remains associated with Spanish royalty and
the House of Aragon (recovered from tombs within the Royal Pantheon of San Juan de
la Peña archaeological site in Spain). She is currently working on the putative remains of
William Townsend Washington, the nephew of former U.S. President George Washington.
In addition to skeletal remains cases and research, Dr. Ambers collaborated with the
National Institute of Justice (NIJ) and the Forensic Technology Center of Excellence (FTCoE)
to develop and disseminate a formal report on the use of familial DNA searching (FDS) in
casework. She is an active cold case consultant, an advocate of postconviction DNA testing,
and an educator/advisor on DNA testing or retesting of old, degraded, or challenging evi-
dentiary samples. Dr. Ambers also was the 2017–18 project lead on a U.S. State Department
grant to combat human trafficking in Central America through the application of foren-
sics. As part of the program objectives, she traveled to three Northern Triangle countries
(Guatemala, El Salvador, Honduras) to perform gap assessments of government laboratories
and train personnel in forensic DNA analysis, with the goal of promoting quality casework
methods based on ISO 17025 standards. As Project Lead, she helped train forensic DNA
scientists at the Instituto Nacional de Ciencias Forenses de Guatemala (INACIF, the National
Institute of Forensic Sciences of Guatemala) in Guatemala City, Guatemala; the Instituto de
Medicina Legal (Institute of Legal Medicine) in San Salvador, El Salvador; and the Instituto
Editor biography xxxv
de Medicina Forenses: Centro de Medicina Legal y Ciencias Forenses (the Institute of Forensic
Medicine: Center of Legal Medicine and Forensic Sciences) in Tegucigalpa, Honduras. In ad-
dition to providing both lecture and tactile training on DNA analysis methods to Northern
Triangle laboratories, Dr. Ambers was part of a consortium to help these countries develop
and maintain forensic DNA databases to assist in the identification of missing persons re-
lated to human trafficking.
Dr. Ambers has mentored hundreds of students during her career and for multiple
years served as the faculty advisor for Scientista, the largest network of college and grad-
uate women innovating science, technology, engineering, and math (STEM) in the United
States. The Scientista Foundation is a national organization that empowers women in science,
technology, engineering, and math (STEM) through content, communities, and conferences.
During her free time, Dr. Ambers enjoys traveling, hiking, kayaking, reading, spending time
with her dogs, and volunteering to help victims of abuse as well as minority and oppressed
populations.
In 2017–18, Dr. Ambers was Project Lead on a U.S. State Department grant to combat human trafficking in Central
America’s Northern Triangle, where she helped train forensic DNA scientists at the Instituto Nacional de Ciencias
Forenses de Guatemala (INACIF, the National Institute of Forensic Sciences of Guatemala) in Guatemala City,
Guatemala; the Instituto de Medicina Legal (Institute of Legal Medicine) in San Salvador, El Salvador; and the Instituto
de Medicina Forenses: Centro de Medicina Legal y Ciencias Forenses (the Institute of Forensic Medicine: Center of Legal
Medicine and Forensic Sciences) in Tegucigalpa, Honduras
xxxvi Editor biography
Dr. Ambers at the 2016 funeral ceremony of unidentified human skeletal remains unearthed by a construction
crew in Deadwood, South Dakota. The remains were discovered in a residential neighborhood that was built atop
the original site of Ingleside Cemetery (Deadwood’s first burial ground, 1876–78). The remains were reinterred in
Mt. Moriah Cemetery, the final resting place of “Wild Bill” Hickok, Calamity Jane, and the City of Deadwood’s
first Sheriff Seth Bullock.
Preface
“I hope my achievements in life shall be are seeking adventure and spiritual enlighten-
these—that I will have fought for what was ment amongst the majesty of the Himalayas.
right and fair, that I will have risked for that One evening, in my room at the Trident
which mattered, that I will have given help to Gurgaon Hotel, I initiated an internet search
those who were in need, and that I will have to learn more about this magical region of
left this Earth a better place for what I've done northern India. Despite its allure to adven-
and who I've been.” ture seekers and spiritualists, and its beauti-
—Carl Hoppe ful landscape, many travelers underestimate
the sheer vastness of the mountain range
The impetus for this book arose during and the unpredictable weather conditions in
an international trip that I took in 2017, on the area. As such, there is a popular hiking
behalf of Thermo Fisher Scientific’s Human region in the Himalayas within Himachal
Identification (HID) University, to train sci- Pradesh (called Parvati Valley) that has been
entists from various states in India and the unofficially nicknamed the “Valley of Death”
Maldives Police Service on the processing because of the large number of American
and extraction of DNA from human skeletal and European tourists who have gone miss-
remains. During my time in India, I was ap- ing while hiking in the area over the past
proached by a forensic DNA specialist from few decades. In fact, the number of missing
Himachal Pradesh, the northernmost state in American and European tourists is in the doz-
India which borders the Himalayan Mountain ens. During my search to learn more about the
Range and is the residence of the Dalai Lama missing tourists, I ran across a website titled
in exile. This scientist’s team had been tasked “The Adventures of Justin,” which chronicled
with identifying a set of skeletal remains dis- the adventures of a 32-year-old man named
covered in the Himalayas along a popular Justin Alexander Shetler, who quit a lucrative
hiking/trekking route—and he had brought job in corporate America, sold most of his
the remains with him to the bone workshop. belongings, and left behind the security of a
Each day, after the workshop training ended, salary and a life of consumerism in search of
we spent the evenings in the Thermo Fisher a life with deeper meaning. As I read Justin’s
Scientific laboratory facility in New Delhi at- posts, I felt a kinship to his expression that
tempting to recover sufficient genetic material experiences are more valuable than material
from the remains in hopes of making a posi- things. On his website, I was particularly
tive identification and returning the remains drawn to a photograph he had posted of the
to the family. During some of the long incuba- Catacombs in Paris, along with the caption
tion periods throughout the DNA extraction “A reminder that we are impermanent. One day
process, discussions ensued about the pos- we will be nothing but bones and stories.” There
sible identity of the decedent. I learned that was a strange irony to this statement, and I re-
Himachal Pradesh is a popular travel destina- member getting goosebumps thinking about
tion for American and European tourists who his insight and the ostensible premonition
xxxvii
xxxviii Preface
of his own impending mortality. Although one potential option was to contact the re-
Justin was a seasoned traveler and an expe- spective Embassies of each foreign national
rienced outdoorsman, he had disappeared in who had disappeared in the “Valley of
the Parvati Valley of the Himalayas in 2016, Death,” to notify the families of the missing
nearly a year before I arrived in India to teach and request reference samples for compar-
the workshop. As I sat on the bed in my hotel ison to the DNA profile obtained from the
room perusing his story on my laptop, I won- remains. However, this option was far from
dered if the remains recovered in the Indian ideal, as only one of those families (or pos-
Himalayas were those of Justin Shetler. sibly none of them) would receive the news
After numerous evenings in the laboratory, that their missing loved one had finally
and after processing a number of different skel- been found. There was a distinct possibil-
etal elements for DNA, a complete short tan- ity that the skeletal remains discovered did
dem repeat (STR) profile was obtained using not belong to any of the missing American
the FBI’s expanded core CODIS loci and the or European tourists. Furthermore, this type
GlobalFiler™ PCR Amplification Kit (Thermo of investigative approach lacks sensitivity to
Fisher Scientific). For this case, we had the families who have already endured years (or
luxury of using state-of-the-art facilities and even decades) of grief regarding their miss-
equipment at the Thermo Fisher Scientific lab- ing loved one. Rather than notifying the fam-
oratory in New Delhi to conduct DNA testing ilies and unnecessarily raising their hopes
on these samples, which should have facili- that their loved one’s remains had finally
tated the process of making a positive identi- been found, I suggested that we first investi-
fication of the remains. Whenever I talk about gate the racial background of the decedent to
this case to colleagues and students—and see if it aligned with the Anglo-European de-
once I disclose that we obtained a complete scriptions of those missing in the “Valley of
DNA profile—the immediate assumption is Death.” Although a forensic anthropologist
that we were able to positively identify this can often predict the race of an unknown indi-
individual. However, at the time of this case, vidual by examining various characteristics
India did not have a national DNA database, of the skull, that possibility was precluded in
nor had they collected reference samples from this case by the partial nature of the skeletal
the families of the missing foreign nationals, remains recovered. The skull was disarticu-
so there was no genetic data available for lated, incomplete, and heavily fragmented.
comparison to aid in the identification or for Fortunately, recent advances in DNA tech-
reassociating the remains with any particular nology provide a mechanism through which
family. This is a common issue encountered to make an assessment of race in partial skel-
in forensic genetic investigations of unidenti- etal remains cases. With additional testing of
fied human remains (UHR). It exemplifies the a panel of ancestry-informative single nucle-
fact that, even when using the most advanced otide polymorphisms (aiSNPs) using mas-
technology and laboratory techniques, the sively parallel sequencing (MPS), and then
process of identification is multifaceted and comparing this aiSNP data to the Forensic
requires that certain infrastructure is in place Research/Reference on Genetics knowledge
in order to be successful. Ultimately, the DNA base (FROG-kb) and the ALele FREquency
profile obtained from the skeletal remains is Database (ALFRED), it was determined that
only one piece of the puzzle. the remains recovered from the hiking trail
At this stage in the investigation (and in the Indian Himalayas did not belong to
armed with a complete 24-locus STR profile), an individual of Anglo-European descent.
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Language: English
JANUARY-JUNE 1914.
By N. BOHR,
or
and