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WHOLE GENOME PROFILING

ASSEMBLING YOUR GENOME SEQUENCE TO THE HIGHEST QUALITY

its a green gene revolution

Sequence-based physical mapping with Whole Genome Proling (WGPTM)

WGP is a proprietary sequence-based physical mapping technology that places sequence tags on individual BAC (125-150 Kb) clones. WGP delivers large DNA fragment contigs that serve as a scaffold to assemble your shot-gun sequences to a high quality genome sequence. WGP physical map aided genome assembly - Signi cantly lowers the number of contigs - Signi cantly increases contig length - Solves problems with repeat regions - Links physical and genetic maps - Links genome sequence to BAC clones - Provides direct access to your genes of interest - Scales irrespective of genome size

Chromosome
BAC1 BAC3 BAC6 BAC5 BAC4 BAC2

(A)

(B)
MseI EcoRI

(C)

MseI

TTAAACTTAGTTAGCTTGGACTAACGAATTCGTAGGCATAGTGACTAGCATTG..TTAA

(D)

BAC1 BAC3

BAC6

(E)

J. van Oeveren et al. (2011). Sequence-based physical mapping of complex genomes by Whole Genome Pro ling. Genome Research 21: 618-625 www.wholegenomepro ling.com

Examples of genomes with WGP aided assembly - Melon (0.4 Gb) - Lettuce (2.6 Gb) highly repetitive - Sun ower (3.6 Gb) large & repetitive - Tobacco (4.6 Gb) tetraploid - Wheat Chromosome by chromosome Now the WGP technology is more affordable than ever by making the technology compatible with the Illumina Hi-Seq 2000 platform, unprecedented data production at signi cantly reduced cost became possible. WGP is offered at three different application levels: - WGP-gold based on 12x BAC library coverage - WGP-standard based on 10x BAC library coverage - WGP-annotated based on 2-6x BAC library coverage

Ask us for a quote for your genome today!

Prof. Rod Wing Arizona Genome Institute I am a strong proponent of using physical maps as backbones for the generation of new reference genomes. Several genomes published without the use of physical maps have more than half the genome missing. Callum Bell National Center for Genome Resources KeyGenes method offers a physical map in a shorter amount of time and at a lower cost compared to other methods. Physical maps go a long way in building bigger scaffolds.

Michiel van Eijk KeyGene To date, we have completed high-quality WGP maps for over 20 crops ranging in genome size between 130 Mbp and 5 Gbp, including highly repetitive diploids such as lettuce and pepper and polyploids such as canola and tobacco. Our results demonstrate unequivocally the versatility of WGP to deliver superior sequence-based physical maps irrespective of genome size and/or repeat content.

References
Next-Generation Sequencing of Bacterial Arti cial Chromosome Clones for NextGeneration Physical Mapping. Tag-based Next Generation Sequencing, First Edition. Edited by Matthias Harbers and Gunter Kahl.2012 Wiley-VCH Verlag GmbH & Co. KGaA. Published 2012 by Wiley-VCH Verlag GmbH & Co. KGaA. Robert Bogden et al. Sequence-based physical mapping of complex genomes by whole genome pro ling Jan van Oeveren et al. Genome Research published online February 1, 2011 A pilot project to assess the ef ciency of whole genome pro ling for physical mapping and sequencing the wheat genome ITMI 2010 abstract, Phillipe Romain et al Construction of Tobacco Physical Map Using Whole Genome Pro ling PAG 2011 abstract. Nicolas Sierro et al. Physical Mapping and Sequencing Of The Sun ower Genome PAG 2011 abstract. Loren Rieseberg et al. Sequence-based physical map construction by whole genome pro ling PAG 2011 abstract. Jan van Oeveren et al

Please contact: KeyGene, for North & South America Mark van Haaren E mark.van-haaren@keygene.com T +1 301 366 2312 I www.keygene.com Or our collaborators: National Center for Genome Resources (NCGR), USA Callum Bell E cjb@ncgr.org T +1 505 982 7840 I www.ncgr.org Arizona Genomics Institute, USA Rod A. Wing E rwing@ag.arizona.edu T +1 520 626 9595 I www.genome.arizona.edu KeyGene, for Europe & Asia Herco van Liere E herco.van-liere@keygene.com T +31 317 466866 I www.keygene.com Amplicon Express Robert Bogden E bogden@genomex.com T +1 509 332 8080 I www.genomex.com