COLAB4FOOD SERVICE PROPOSAL

DATE: 15/07/2021

PROPOSAL SCOPE
Company <Empresa do cliente>
Represented by Vitor Menas
VAT number
Address
Postal code. / Location Mértola
Service Scope Food safety and shelf-life analysis
Proposal (Nr) 1332021
Proposal validity 30 days
Please confirm your intent on this service by replying back to:
Observation geral@colab4food.com and miguel.teixeira@colab4food.com

PROPOSAL DETAILS
Subject Food safety and shelf-life analysis

Sample collection can be at the responsibility of one of our laboratory

Sample collection
technicians or alternatively, it can be carried out by the customer.

Our laboratory has all the material suitable for carrying out the
required formulation tests.
Sampling
In view of the COVID-19, our laboratory provides its technicians with
material the following equipment for individual protection: masks, visors,
gloves and hand sanitizer.

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 WORKPLAN
Objectives:

Vitor Menas wishes to export acorn flour to a Swiss customer, ensuring

the food safety of the product.
The client has the acorn flour certified as organic (Reg. (EU) 848/2018), for
which GMO and ionizing radiation absence are requirements. In this sense,
GMO (Reg. (EC) nº 1829/2003 & 1830/2003), ionizing radiation (Directive
1999/2/EC & 1999/3/EC) and traceability (Reg. (EC) nº 178/2002) regulations
are ensured.
The industrial facility in which the acorn flour is processed is allergen free
(Reg. (EU) nº 1169/2011).

The client also requests the nutritional declaration (Reg, (EU) 1169/2011) and
the shelf-life assessment of the product.

Tasks:
Colab4Food will perform the following analysis (adaptable to the client’s
needs):
1. Nutritional analysis:
➢ Sodium (Atomic absorption spectroscopy - AAS)
➢ Total ash (Thermogravimetric method)
➢ Moisture (Thermogravimetric method)
➢ Sugar (NP 1420 - Luff-Schoorl method)
➢ Total fat (NP 4168)
➢ Lipid Profile (saturated, monounsaturated, polyunsaturated (GC-FID)
➢ Protein (Combustion method)
➢ Salt (calculation)
➢ Carbohydrates (calculation)
➢ Energy value (calculation)

2. Heavy metals: cadmium and lead (Graphite furnace atomic absorption

spectroscopy - GFAAS)

3. Pesticides: determination of pesticide traces (2,4-DDD; 4,4-DDD; 2,4-DDE;

2,4-DDT; 4,4-DDT; Tetradifão; Beta-BHC; Acrinatrina; Antraquinona;
Bifentrina; Lambda-Cialotrina e Pirimifos-Metilo) (GC-MS/MS + LC-
MS/MS)

4. Micotoxins: aflatoxins (B1+B2+G1+G2), Ochratoxin A, Zearalenone,

Vomitoxin / Deoxynivalenol (DON) (LC-MS/MS)

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 5. Shelf-life assessment:
Samples will be stored at 15ºC and analysed initial time (0 months), and
every 3 months to detect and enumerate the following
microorganisms (time 0, 3, 6, 9, and 12 months):
➢ microorganisms 30ºC
➢ molds and yeasts
➢ Enterobacteriaceae
➢ Bacillus cereus
These trials will be interrupted in case of chemical and/or sensory
deterioration of the products (5. Chemical and sensory stability).

6. Chemical and sensory stability:

During the expected storage time and conditions (12 months at 15ºC),
the following determinations will be conducted:
- Sensory evaluation (colour – visual and instrumental; aroma, taste and
overall appreciation)
- Fat acidity (ISO 3960) and peroxide value (NP 2967) of the flour
These trials will be conducted at initial time (0 months) and every 2
months (time 0, 2, 4, 6, 8, 10 and t12).
In case of chemical and/or sensory deterioration of the samples these
trials will be interrupted.

These analyses will be performed on acorn flour samples provided by the

client.

Outputs delivered by Colab4Food:

• Product labelling requirements and nutritional declaration (Reg. (EU)

1169/2011)
• Report with:
➢ Heavy metals (Reg. (EC) 1881/2006)
➢ Pesticides
➢ Micotoxins
➢ Microbiological analysis
➢ Chemical analysis
• Report with:
➢ Shelf-life assessment (12 months)
➢ Chemical and sensory stability (12 months)

• As many meetings/interactions needed between the client and

Colab4Food.

Success criteria:

Report delivery on time (15 days after completion of the service).

Customer’s positive evaluation (customer satisfaction survey).

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 GENERAL BUDGET / FINANCIAL INVESTIMENT
The following table contains the price list for the provision of the services
described in this proposal.

Services Price un (€)/sample Price un (€)/ anual /sample

Service 1

1. Nutritional analysis 159.50

2. Heavy metals analysis 23.00

3. Pesticide analysis 91.00

4. Micotoxin analysis 185.00

5. Microbiology analysis 17.00

6. Chemical and sensory analysis 99.60

Subtotal 1 575.10

Service 2 Price un (€)/sample

7. Shelf-life assessment 17.00 68.00

8. Chemical and sensory stability 99.60 597.60

Subtotal 2 665.60

Total (Subtotal 1 + Subtotal 2) 1240.70

CONDITIONS OF SERVICE PROVISION

100% of the total value of the Service 1 should be paid out

upon adjudication of the service, and the remaining value
(service 2) upon delivery of the Final Report, through bank
Payment conditions
transfer.
Prices are excluding taxes (IVA) according to the
Portuguese Law.
We ensure complete confidentiality of the data produced
Confidentiality and discussed with the client. A non-disclosure agreement
can be signed between the parties.

Thank you for choosing Colab4Food!

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 In case you may have any questions, please let us know.

Best regards,

MIGUEL TEIXEIRA

Scientific & Executive Director

T + 351 936268137
colab4food.com
INIAV – Polo de Vairão, Rua dos Lagidos
4485-655, Vairão, Vila do Conde, Portugal
LinkedIn | Facebook

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