AVSEEL ( Avocado Seed Kernel Spray as Substance for Synthetic

Mosquito Repellent )
Ellianne Danette (Leader), Khairunnisa Keisha Anjani, Nadia Herlia Octaviany,
Rafi Syahputra Lamikan, Salsabila Nauraswari Johan, Zaftio Apreda Akmal
Supervisor : Budi Santoso, M.Pd., C.Ht., Laily Eka Pradina, S.Pd., Drs. Suyono

Email : danetteelianne@gmail.com

Pemerintah Provinsi Jawa Timur

Dinas Pendidikan Provinsi Jawa Timur
SMA Negeri 10 Surabaya
Jalan Jemursari I No. 28 SURABAYA 60237
 ABSTRACT
AVSEEL ( Avocado Seed Kernel Spray as Substance for Synthetic
Mosquito Repellent )
Ellianne Danette (Leader), Khairunnisa Keisha Anjani, Nadia Herlia Octaviany,
Rafi Syahputra Lamikan, Salsabila Nauraswari Johan, Zaftio Apreda Akmal
Supervisor : Budi Santoso, M.Pd., C.Ht., Laily Eka Pradina, S.Pd., Drs. Suyono
danetteelianne@gmail.com
Based on the results of one study, it is known that the use of mosquito
coils has a greater effect on tackling disease than the use of larvicides and
fogging. Most of the insect repellent sprays available on the market today contain
the active ingredient diethyltoluamide (DEET) which is harmful to the skin. That's
why we developed an alternative fruit-based mosquito repellent. In order to
eliminate the use of active chemicals that are harmful to the body. The fruit source
we use is avocado (Persea americana). We use the seeds of the fruit as an active
ingredient to make mosquito repellents. This is because avocado seeds contain
flavonoids, saponins, and tannins. These compounds will be the active mosquito
killer compounds in our study. Our study aimed to determine the effectiveness of
mosquito repellents based on avocado seed extract on Culex sp. mosquitoes. The
research design is a qualitative posttest. The research object was Culex sp.
mosquitoes aged 2-3 days, totaling 25 in each treatment. The treatment presented
was spray with a concentration of 10%,15%,20%,25%, and 30% avocado seed
extract and eventually showed that the spray with a concentration of 25% was the
most effective. The data came to a proof that avocado seed extract can effectively
repel mosquitoes.

Keywords : Culex sp., Persea Americana, Flavonoids, Saponins, Tannins

1. Introduction
Anti-mosquito drugs are a
medium by which people control the
spread of mosquitoes which are
produced in several forms, such as
coils, spray, vaporization, or other
preparations (Soderlund and
Bloomquist, 1989). However, most
of these mediums contain chemical
compounds that can cause significant
health problems, such as respiratory
problems, nervous disorders, brain
damage, and even death. In addition,
the majority of mosquito repellents
around us contain the compound
diethyltoluamide (DEET). The
negative effects of this compound
include inviting skin irritation, and
can even cause symptoms of seizures
for people with encephalopathy
(Daniel R. S., 2019).
Therefore, a synthetic mosquito
repellent substance is needed which
is usually obtained from organic
extracts, so that it makes mosquito
repellents natural and herbal.
Unfortunately, after people
consume fruit, they tend to throw
2. Method and Experimental
Details
Experiment method and details
The research design we use is a
quantitative research method based on
data.
The methods we use are Soxhlet
and evaporation. We chose this
method because it has advantages
compared to other methods.
The tools we use in this research
process are Analytical scales, a
mincer (mitochiba), an oven, a beaker
glass, a thermometer (Celsius), a
measuring cup, and there is a
condenser/cooler, an extraction
away fruit seeds and lead to an
increase in fruit seed waste in the
surrounding environment.
Unbeknownst to them, fruit seeds
also contain various compounds that
can have a positive impact, both in
terms of the environment and health.
Referring to the above issues, the
formulation of the problem was
obtained regarding the effectiveness
of mosquito repellent with avocado
seed extract on the mortality of Culex
sp. mosquitoes. Some natural
compounds as mosquito repellants
that are often contained in fruits are
flavonoids, saponins, and tannins.
These three compounds can be found
in the flesh of the avocado seed
(Persea Americana). Flavonoids are
effective as inhibitors of insect
appetite because they are toxic.
This study aims to provide a
solution to synthetic mosquito
repellents by utilizing organic waste
which is useful as a basic ingredient
for mosquito repellents, such as
avocado seeds.
chamber, a round bottom flask, a
heater, clamps, states, where water
enters the condenser, where the water
exits the condenser, water hose, and
siphon for the Soxhlet process and
rotary evaporator for the evaporation
process.
The materials we used in this
research process were: dusty filter
paper, avocado seeds (Persea
americana) obtained from fruit seed
sellers in Surabaya City (the criteria
for avocado seeds that we chose were
fresh seeds and hard shells), then
chemicals used in this study were
hexanal and aquadest solutions as
solvents in spray preparations.
The sample in this study was
Culex sp. Obtained at the ornamental
fish shop on Jl. Patua in Surabaya are
still in the form of larvae which will
be bred in an experimental laboratory.
Research procedure
1. Preparation of avocado seed extract
As much as 500 grams of
avocado seed powder was extracted
using the distilation method. The
sample to be extracted is usually a
finely ground solid. This solid is then
wrapped in filter paper and then put in
a distinct distilation apparatus. The
distilation tool was installed, then a
sample of 100 grams of mashed dry
avocado seed extract was wrapped in
filter paper then tied with thread, and
put into the distilation tool. Enter 500
ml of 96% ethanol solvent into the
distilation flask. Perform l until the
cycle drops are colorless again, the
liquid extract obtained is then
concentrated using a rotary
evaporator. This process takes place
continuously. The next stage is
evaporation using a rotary evaporator.
Evaporation is carried out by
evaporating some of the solvents at
their boiling point so that a
concentrated liquid solution with a
higher concentration is obtained.
2. Phytochemical Screening
The next stage is the test for
Flavonoids, Saponins, and Tannins in
avocado seed extract in the lab by
taking samples from avocado seed
extract.
3. Manufacture of spray products as
mosquito repellents
The next stage is mixing
avocado seed extract with Aquadest
with a concentration of 5%; 10%;
15%; 17% and 20%
4. Product Testing Spray as an
anti-mosquito
Testing of anti-mosquito spray
aims to determine the effectiveness of
spray from avocado seed extract as an
anti-mosquito. This test was carried
out in an experimental medium which
was divided into 5 groups based on
the concentration of the ingredients to
determine its effectiveness. A total of
25 mosquitoes were put into the
experimental medium and then given
avocado seed extract in the form of a
spray. The number of mosquitoes that
died and the time it took would be
recorded and a comparison was made
to determine which spray with the
most effective extract concentration
was used as an anti-mosquito in this
study.
3. Result and Discussion
Our research has shown that
avocado seed extract in the form of an
anti-mosquito spray can cause the
death of Culex sp. This can be seen
from the percentage of Culex sp. in
each test group presented in the figure
below.
The figure shows a sharp increase
in mosquito deaths in the group
exposed to distilled water and the
group exposed to avocado seed Soderlund and Bloomquist, 1989
extract. Meanwhile, in the treatment D.M. Soderlund, J.R.
group exposed to avocado seed Bloomquist Neurotoxic
extract, it can be concluded that as the actions of pyrethroid
concentration of avocado seed extract insecticides Annu. Rev.
increased, the percentage of Culex sp. Entomol., 34(1), 77-96
mosquito deaths also increased.
Therefore, the highest percentage Stuyft, Prof PVd 1999, Urbanisasi
of deaths in the treatment group was Demam Kuning di Santa Cr uz
in the avocado seed extract at a Bolivia,The lancet, Vol.
concentration of 25 %. 353(9624), 1558-1562
4. Conclusion
Based on the results of this study, it Swale, Daniel R., Jeffrey R.
can be concluded that the seed extract Bloomquist, 2019, Is DEET a
of avocado (Persea americana) in the Dangerous Neurotoxitant
form of an anti-mosquito spray has the
power to kill Culex sp. Utomo.PP,NanaSupriyatna,2014,Per
bandingan Daya Proteksi
5. Acknowledgments Losion Anti Nyamuk Dari
Acknowledgments are extended to Beberapa Jenis Minyak Atsiri
the Pharmacy Academy, SMAN 10 Tanaman Pengusir
Surabaya, our parents and Nyamuk,Biopropal Industr
supervisors, and the journal authors
,Vol. 5(2),79-84
who have helped this research
morally and materially. Wahyonoa,TYM, Oktarinda
MWB,2016, Penggunaan Obat
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