COURSE CURRICULUM

Course Title: BIOPROCESS TECHNOLOGY L T P/S SW/FW TOTAL

CREDIT
UNITS
Course Code: BCE402 2 - 2 - 3
Credit Units: 3
Level: UG (B.Tech BT)

Course Objectives:

Theory: The objective of the course is to apply the principles of biochemical engineering in production of industrially important products economically.

Practical: Students will trained for isolation and culture of industrial important microorganism, determination of Thermal Death Point and Thermal Death Time,
microbial growth kinetics, estimation of reducing sugar and ethanol, operation bioreactor, and produce ethanol by fermentation

Pre-requisites: Knowledge of Chemical Engineering Principles and Biochemical Engineering

Course Learning Outcomes:

• Understand the media formulation and design for the fermentation processes
• Apply the techniques of upstream and downstream processes for the production of primary metabolites
• Integrate the knowledge of purification processes for the large scale production of secondary metabolites
• Demonstrate a clear understanding of the production process for industrially important enzymes
Course Contents:

Weightage (%)
Module I 30

Introduction to Bioprocess Technology and Media Design for Fermentation

Bioprocess vs chemical processing, advantages, disadvantages, Substrates for bioconversion process, Isolation,
Preservation Techniques and Maintenance methods of Industrial Microorganisms, Cell culture technique, Media
composition and design, Media type, Commercial media, Inoculum development and aseptic transfers, Criteria for
inoculums transfer.
Module II 30
Process Technology for Production of Primary Metabolites
Ethanol: production by batch and continuous process by various technologies. Determination of fermentation
efficiency, distillation efficiency and overall efficiency of ethanol production, recovery, uses, glucose effect etc. Power
alcohol – definition, uses, merits and demerits of various technologies for its production.
Amino Acid:
Lysine: Indirect and direct fermentation
Biomass: Bakers and distillers yeast production using various raw materials, “bios” factors for growth, Crabtree effect,
harvesting, different forms and uses.
Different forms of common mushroom production from agro based raw materials and uses. Biofertilizers,
Module III 20
Process Technology for Production of Secondary Metabolites
Production of secondary metabolites – penicillin, streptomycin,
Penicillin: Classification, various penicillin as precursor and ‘R’ – side chain, penicillinase, 6-APA, penicillin
production, harvest and recovery, uses of various forms etc.
Streptomycin: chemical structure, production, harvest and recovery, use by-product of streptomycin fermentation etc.

Module IV 20
Microbial production of industrial enzymes
Glucose isomerase, , Amylase, Lipase, Protease
Pedagogy for Course Delivery:

Lectures: 20
Presentation/Seminar/Quiz: 2
Class Test:4
Guest Lecture/Industry/Academia:4
Total: 30

Lab/ Practical details, if applicable:

Practical: 26
Class Test: 2
Viva/Quiz: 2
Total: 30

List of Experiments:

• Isolation of industrially important bacteria for microbial processes.

• Estimation of Reducing sugar by DNS method of unknown sample and establish calibration curve.
• Estimation of ethanol by potassium dichromate methods and establish calibration curve.
• Establish growth profile of bacteria with respect of optical density and maximum specific growth rate of bacteria
• Determination of Thermal Death Point and Thermal death time of bacteria for design of a sterilizer.
• Cultivation of microorganism in batch process
• Ethanol production using grape juice
• Demonstration of bioreactor

Assessment/ Examination Scheme:

Theory L/T (%) Lab/Practical/Studio (%) Total

67 33 100%
Theory Assessment (L&T):
Continuous Assessment/Internal Assessment

Component
End Term
Mapping Class Test I Home Presentation Class Attendance
Examination
Continuous and II assignment Participation
Evaluation
components
with CLOs:
(Drop down)

Weightage
(%) 20 10 10 5 5 50

Lab/ Practical/ Studio Assessment:

Continuous Assessment/Internal End Term Examination
Assessment
Components Performance Lab viva Class Attendance Lab Performance Viva Total
(Drop down record Participation record
Weightage 20 10 10 5 5 10 20 20 100
(%)
Mapping Continuous Evaluation components with CLOs:
Course Learning CLO1 CLO2 CLO3 CLO4
Outcomes

Assessment type
/PSDA
Module Assessment1 √ √ √

Module assessment 2 √ √ √

Module assessment 3 √ √ √

Module assessment 4 √ √

Home Assignment-1 √ √ √

Home assignment-2 √ √ √

Viva /Quiz-2 √ √ √

Class √ √ √ √
Presentation/Seminar
Text & References:
Text:
• Bioprocess Engineering Basic Concepts, M.L. Shuler and Fikret. Kargi, 2013, 2nd edition, Pearson Education Limited. ISBN : 9780136060659
• Biotechnology: A Textbook of Industrial Microbiology, W. Crueger and A. Crueger,1990, Sinauer Associates. ISBN : 0878931317

References:

• Text Book of Biotechnology H. K. Das, 2007, Third edition, John Wiley & Sons. ISBN:9788126510146
• Industrial Microbiology Casida JR., 2000, New Age International (P) Ltd. New Delhi. ISBN: 9788173718892