Professional Documents
Culture Documents
DATASHEET EXP 2
DATASHEET EXP 2
BIO 270
LAB ACTIVITY DATA SHEET
We declare that the data worksheet submitted is not plagiarised and is entirely our own work, and
that no part of it has been copied from any work produced by other person(s)/ source(s) or provided
by any other student(s). We understand that issuing a false declaration can result in severe penalties
and we are willing to be penalized if any form of copying is found valid.
Endorsed by:
1
BIO270 / OCT 2023 - FEB 2024
Instructions:
1. This lab activity datasheet must be completed and submitted to the lecturer in charge
for endorsement for every lab activity.
2. Print out the data sheet according to the date and time of lab activity.
3. Every member of the group may use other reliable information to assist each other to
complete the data sheet.
4. Should the group need any further clarification about the lab activity, please consult
with the lecturer in charge.
5. Answer ALL questions in the datasheet. DO NOT alter this workbook, please use the
box given to write your answer accordingly.
6. Failure to submit this workbook for the lecturer’s endorsement may affect the
continuous assessment marks.
2
BIO270 / OCT 2023 - FEB 2024
LAB ACTIVITY 3
DISSECTION OF ANIMAL
TOPIC LINK
https://youtu.be/2Du0h270a-c
3. Dissection https://youtu.be/9Xk2KCYTujk
procedure
3
BIO270 / OCT 2023 - FEB 2024
Mice
Zebrafish
Dog
Monkey
4
BIO270 / OCT 2023 - FEB 2024
HUMANE KILLING
1. After some procedures, it may be necessary to kill the animal to prevent further
suffering. The person who conducted the procedure must ensure that the animal is
killed competently and humanely. List out the appropriate methods used in humane
killing for the animals in research. Refer to
https://www.understandinganimalresearch.org.uk/what-is-animal-research/understandi
ng-animal-procedures/reducing-suffering/
a) Anesthetic overdose
b) Exposure to carbon dioxide gas
c) Dislocation of the neck
d) Concussion followed by the destruction of the brain
e) Destruction of the brain by a free bullet (for hoofed animals only)
5
BIO270 / OCT 2023 - FEB 2024
DISSECTION PROCEDURE
1. Before dissection, identify the parts of the external anatomy of the animal. Attach the
images that you have taken during the experiment in the box provided below showing
appropriate labels and fill in the blanks.
Nares
Incisors Vibrissae
Cranial region
Chin
Forearm
Cervical region
Forelimbs
Thorax Elbow
Nipples
Abdomen
Thigh
Pelvic region
Knee
Hindlimb
Vulva
Tail
Anus
6
BIO270 / OCT 2023 - FEB 2024
Trachea
Lung
Heart
Ribs Liver
Stomach
Spleen
Small Cecum
intestine
Pancreas
Urinary Anus
bladder
Figure 1: Internal anatomy showing thoracic organs, abdominal organs and, excretory organs
7
BIO270 / OCT 2023 - FEB 2024
3. Discuss with your members of the group to fill in the columns with the appropriate
answers.
4. From the observation on the dissected animal, attach the image showing the
differences between the parts of the organ for both male and female reproductive
systems with appropriate labels. Understand the differences between both male and
female reproductive systems.
Male Female
8
BIO270 / OCT 2023 - FEB 2024
6. The carcass must be disposed of properly. By referring to the video, describe how you
would dispose of the carcass in the lab. https://youtu.be/VDiGd4620ac
By, put the carcass in biohazard bag and sealed as soon as possible. There are three common
effective methods of carcass disposal which are incineration, burying, and rendering.
Incineration is the method that always used when dealing with the carcass especially when the
carcass had diseased; however, it can also be the most expensive. An acceptable alternative is to
bury the carcass. The carcass would be buried at least 4 feet deep and covered with lime to
discourage scavengers from uncovering and consuming it.
9
BIO270 / OCT 2023 - FEB 2024
REFERENCES
1. NexGen Pharmaceuticals (18 November 2019). What is the role of a veterinarian?. NexGen
Animal Health.
https://nexgenvetrx.com/blog/what-role-veterinarian/
2. Tianjuang Wang, Lidia Oltra-Rodríguez, Nuria García-Carrillo, Anibal Nieto, Yunxia Cao, and
María L. Sánchez-Ferrer (2 December 2017). Ultrasonography in Experimental Reproductive
Investigations on Rats. National Library of Medicine.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755523/
4. Péter Lőw, Kinga Molnár & György Kriska (4 May 2016). Dissection of the Rat (Rattus
norvegicus). Springer Link.
https://link.springer.com/chapter/10.1007/978-3-319-25172-1_12
10
BIO270 / OCT 2023 - FEB 2024
ACKNOWLEDGEMENT
We would like to thank all the teaching team and lecturers in charge of BIO270 Animals and
Plants Physiology UiTM for their expertise and suggestions in ensuring these assessment
activities are a success. Here, we also acknowledge, with special thanks, to Dr Nursakinah
Latifi and Mr Muhammad Fadhli Mohd Azahar from Laboratory Animal Facility and
Management (LAFAM) as well as Madam Nina Keterina Hashim from Faculty of Health
Sciences, UiTM Puncak Alam for their involvement and production of the video demo.
REFERENCES
https://www.youtube.com/watch?v=U9OiEAWbU-M
https://www.understandinganimalresearch.org.uk/animals/three-rs/
Thank You
11